This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.14 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 264,820 (7.6%) Reads that were too short: 16,255 (0.5%) Reads written (passing filters): 248,565 (7.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 69,669,380 bp (6.7%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 264820 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3392 847.3 0 3392 7 111874 847.3 0 111874 8 2976 847.3 0 2976 9 102204 847.3 0 102204 10 137 847.3 0 137 11 43 847.3 0 43 12 70 847.3 0 70 13 61 847.3 0 61 14 31 847.3 0 31 15 47 847.3 0 47 16 48 847.3 0 48 17 70 847.3 0 70 18 63 847.3 0 63 19 31 847.3 0 31 20 43 847.3 0 43 21 40 847.3 0 40 22 38 847.3 0 38 23 38 847.3 0 38 24 32 847.3 0 32 25 44 847.3 0 44 26 38 847.3 0 38 27 56 847.3 0 56 28 69 847.3 0 69 29 61 847.3 0 61 30 57 847.3 0 57 31 50 847.3 0 50 32 57 847.3 0 57 33 78 847.3 0 78 34 56 847.3 0 56 35 47 847.3 0 47 36 53 847.3 0 53 37 70 847.3 0 70 38 57 847.3 0 57 39 68 847.3 0 68 40 94 847.3 0 94 41 70 847.3 0 70 42 68 847.3 0 68 43 77 847.3 0 77 44 141 847.3 0 141 45 202 847.3 0 202 46 379 847.3 0 379 47 48 847.3 0 48 48 50 847.3 0 50 49 38 847.3 0 38 50 72 847.3 0 72 51 90 847.3 0 90 52 59 847.3 0 59 53 81 847.3 0 81 54 54 847.3 0 54 55 35 847.3 0 35 56 69 847.3 0 69 57 109 847.3 0 109 58 494 847.3 0 494 59 286 847.3 0 286 60 198 847.3 0 198 61 50 847.3 0 50 62 39 847.3 0 39 63 40 847.3 0 40 64 34 847.3 0 34 65 43 847.3 0 43 66 55 847.3 0 55 67 60 847.3 0 60 68 42 847.3 0 42 69 50 847.3 0 50 70 33 847.3 0 33 71 38 847.3 0 38 72 44 847.3 0 44 73 64 847.3 0 64 74 68 847.3 0 68 75 83 847.3 0 83 76 47 847.3 0 47 77 63 847.3 0 63 78 49 847.3 0 49 79 58 847.3 0 58 80 61 847.3 0 61 81 78 847.3 0 78 82 63 847.3 0 63 83 83 847.3 0 83 84 63 847.3 0 63 85 58 847.3 0 58 86 58 847.3 0 58 87 48 847.3 0 48 88 61 847.3 0 61 89 87 847.3 0 87 90 91 847.3 0 91 91 129 847.3 0 129 92 100 847.3 0 100 93 130 847.3 0 130 94 102 847.3 0 102 95 71 847.3 0 71 96 101 847.3 0 101 97 103 847.3 0 103 98 68 847.3 0 68 99 86 847.3 0 86 100 60 847.3 0 60 101 88 847.3 0 88 102 65 847.3 0 65 103 73 847.3 0 73 104 68 847.3 0 68 105 126 847.3 0 126 106 562 847.3 0 562 107 1071 847.3 0 1071 108 873 847.3 0 873 109 86 847.3 0 86 110 72 847.3 0 72 111 74 847.3 0 74 112 66 847.3 0 66 113 84 847.3 0 84 114 666 847.3 0 666 115 430 847.3 0 430 116 352 847.3 0 352 117 52 847.3 0 52 118 69 847.3 0 69 119 64 847.3 0 64 120 52 847.3 0 52 121 78 847.3 0 78 122 65 847.3 0 65 123 83 847.3 0 83 124 92 847.3 0 92 125 80 847.3 0 80 126 107 847.3 0 107 127 138 847.3 0 138 128 393 847.3 0 393 129 453 847.3 0 453 130 130 847.3 0 130 131 75 847.3 0 75 132 88 847.3 0 88 133 77 847.3 0 77 134 230 847.3 0 230 135 219 847.3 0 219 136 410 847.3 0 410 137 247 847.3 0 247 138 208 847.3 0 208 139 263 847.3 0 263 140 520 847.3 0 520 141 557 847.3 0 557 142 142 847.3 0 142 143 152 847.3 0 152 144 138 847.3 0 138 145 105 847.3 0 105 146 110 847.3 0 110 147 186 847.3 0 186 148 2456 847.3 0 2456 149 1328 847.3 0 1328 150 1392 847.3 0 1392 151 64 847.3 0 64 152 73 847.3 0 73 153 65 847.3 0 65 154 86 847.3 0 86 155 202 847.3 0 202 156 367 847.3 0 367 157 274 847.3 0 274 158 92 847.3 0 92 159 56 847.3 0 56 160 69 847.3 0 69 161 96 847.3 0 96 162 196 847.3 0 196 163 301 847.3 0 301 164 346 847.3 0 346 165 586 847.3 0 586 166 72 847.3 0 72 167 63 847.3 0 63 168 59 847.3 0 59 169 62 847.3 0 62 170 83 847.3 0 83 171 41 847.3 0 41 172 40 847.3 0 40 173 61 847.3 0 61 174 57 847.3 0 57 175 58 847.3 0 58 176 51 847.3 0 51 177 61 847.3 0 61 178 55 847.3 0 55 179 75 847.3 0 75 180 136 847.3 0 136 181 148 847.3 0 148 182 172 847.3 0 172 183 110 847.3 0 110 184 92 847.3 0 92 185 98 847.3 0 98 186 76 847.3 0 76 187 51 847.3 0 51 188 60 847.3 0 60 189 64 847.3 0 64 190 81 847.3 0 81 191 83 847.3 0 83 192 76 847.3 0 76 193 87 847.3 0 87 194 79 847.3 0 79 195 57 847.3 0 57 196 65 847.3 0 65 197 80 847.3 0 80 198 69 847.3 0 69 199 66 847.3 0 66 200 62 847.3 0 62 201 53 847.3 0 53 202 109 847.3 0 109 203 198 847.3 0 198 204 227 847.3 0 227 205 74 847.3 0 74 206 66 847.3 0 66 207 59 847.3 0 59 208 68 847.3 0 68 209 94 847.3 0 94 210 63 847.3 0 63 211 51 847.3 0 51 212 58 847.3 0 58 213 87 847.3 0 87 214 106 847.3 0 106 215 141 847.3 0 141 216 130 847.3 0 130 217 84 847.3 0 84 218 63 847.3 0 63 219 58 847.3 0 58 220 65 847.3 0 65 221 54 847.3 0 54 222 71 847.3 0 71 223 73 847.3 0 73 224 79 847.3 0 79 225 78 847.3 0 78 226 67 847.3 0 67 227 69 847.3 0 69 228 84 847.3 0 84 229 69 847.3 0 69 230 43 847.3 0 43 231 82 847.3 0 82 232 142 847.3 0 142 233 184 847.3 0 184 234 198 847.3 0 198 235 110 847.3 0 110 236 95 847.3 0 95 237 86 847.3 0 86 238 73 847.3 0 73 239 70 847.3 0 70 240 79 847.3 0 79 241 58 847.3 0 58 242 107 847.3 0 107 243 122 847.3 0 122 244 104 847.3 0 104 245 89 847.3 0 89 246 84 847.3 0 84 247 92 847.3 0 92 248 122 847.3 0 122 249 66 847.3 0 66 250 61 847.3 0 61 251 98 847.3 0 98 252 142 847.3 0 142 253 220 847.3 0 220 254 131 847.3 0 131 255 113 847.3 0 113 256 112 847.3 0 112 257 118 847.3 0 118 258 104 847.3 0 104 259 101 847.3 0 101 260 113 847.3 0 113 261 298 847.3 0 298 262 284 847.3 0 284 263 145 847.3 0 145 264 85 847.3 0 85 265 110 847.3 0 110 266 103 847.3 0 103 267 106 847.3 0 106 268 87 847.3 0 87 269 141 847.3 0 141 270 201 847.3 0 201 271 878 847.3 0 878 272 608 847.3 0 608 273 324 847.3 0 324 274 176 847.3 0 176 275 260 847.3 0 260 276 93 847.3 0 93 277 119 847.3 0 119 278 148 847.3 0 148 279 140 847.3 0 140 280 156 847.3 0 156 281 140 847.3 0 140 282 95 847.3 0 95 283 151 847.3 0 151 284 168 847.3 0 168 285 137 847.3 0 137 286 100 847.3 0 100 287 91 847.3 0 91 288 101 847.3 0 101 289 78 847.3 0 78 290 147 847.3 0 147 291 197 847.3 0 197 292 211 847.3 0 211 293 204 847.3 0 204 294 279 847.3 0 279 295 779 847.3 0 779 296 1180 847.3 0 1180 297 1133 847.3 0 1133 298 611 847.3 0 611 299 91 847.3 0 91 300 76 847.3 0 76 301 60 847.3 0 60 Finished in 18.45 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 248,565 Reads with adapters: 113,314 (45.6%) Reads that were too short: 101 (0.0%) Reads written (passing filters): 113,213 (45.5%) Total basepairs processed: 69,669,380 bp Total written (filtered): 31,316,560 bp (45.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 113314 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 17 0.0 2 0 0 0 17 15 52 0.0 3 0 0 42 10 16 945 0.0 3 0 834 83 28 17 110809 0.0 3 105075 4793 741 200 18 393 0.0 3 57 274 31 31 19 281 0.0 3 75 14 108 84 20 427 0.0 3 0 1 0 426 21 3 0.0 3 0 0 0 3 22 3 0.0 3 1 0 0 2 23 1 0.0 3 0 0 0 1 27 3 0.0 3 1 0 0 2 28 2 0.0 3 0 0 0 2 30 3 0.0 3 1 0 1 1 36 1 0.0 3 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 43 121 0.0 3 0 0 0 121 46 15 0.0 3 0 0 1 14 48 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 63 3 0.0 3 0 0 0 3 64 15 0.0 3 0 0 0 15 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 13 0.0 3 0 0 1 12 70 34 0.0 3 0 0 0 34 71 1 0.0 3 0 0 0 1 73 7 0.0 3 0 0 0 7 78 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 9 0.0 3 0 0 0 9 114 6 0.0 3 0 0 0 6 116 5 0.0 3 0 0 0 5 117 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 1 122 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 2 0.0 3 1 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 9 0.0 3 0 0 1 8 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 1 1 164 5 0.0 3 0 0 0 5 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 1 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 3 0.0 3 1 0 0 2 192 3 0.0 3 0 0 0 3 194 6 0.0 3 0 0 0 6 205 6 0.0 3 0 0 0 6 206 1 0.0 3 0 0 1 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 1 1 238 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 5 0.0 3 0 0 0 5 281 6 0.0 3 0 0 0 6 282 6 0.0 3 0 0 0 6 283 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.51 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 113,213 Reads with adapters: 769 (0.7%) Reads written (passing filters): 113,213 (100.0%) Total basepairs processed: 31,316,560 bp Total written (filtered): 31,314,136 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 769 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.0% C: 24.7% G: 14.0% T: 5.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 689 1769.0 0 689 4 54 442.2 0 54 5 16 110.6 0 16 6 9 27.6 0 9 7 1 6.9 0 1