This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.26 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 260,209 (7.5%) Reads that were too short: 9,932 (0.3%) Reads written (passing filters): 250,277 (7.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 70,751,622 bp (6.8%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 260209 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2115 847.3 0 2115 7 111163 847.3 0 111163 8 2892 847.3 0 2892 9 112075 847.3 0 112075 10 104 847.3 0 104 11 38 847.3 0 38 12 38 847.3 0 38 13 42 847.3 0 42 14 27 847.3 0 27 15 13 847.3 0 13 16 44 847.3 0 44 17 51 847.3 0 51 18 27 847.3 0 27 19 24 847.3 0 24 20 24 847.3 0 24 21 17 847.3 0 17 22 25 847.3 0 25 23 20 847.3 0 20 24 25 847.3 0 25 25 34 847.3 0 34 26 23 847.3 0 23 27 16 847.3 0 16 28 31 847.3 0 31 29 40 847.3 0 40 30 40 847.3 0 40 31 27 847.3 0 27 32 28 847.3 0 28 33 53 847.3 0 53 34 31 847.3 0 31 35 26 847.3 0 26 36 32 847.3 0 32 37 27 847.3 0 27 38 34 847.3 0 34 39 29 847.3 0 29 40 55 847.3 0 55 41 44 847.3 0 44 42 47 847.3 0 47 43 40 847.3 0 40 44 95 847.3 0 95 45 111 847.3 0 111 46 285 847.3 0 285 47 36 847.3 0 36 48 29 847.3 0 29 49 29 847.3 0 29 50 36 847.3 0 36 51 43 847.3 0 43 52 35 847.3 0 35 53 34 847.3 0 34 54 30 847.3 0 30 55 27 847.3 0 27 56 26 847.3 0 26 57 33 847.3 0 33 58 196 847.3 0 196 59 89 847.3 0 89 60 52 847.3 0 52 61 31 847.3 0 31 62 34 847.3 0 34 63 20 847.3 0 20 64 30 847.3 0 30 65 34 847.3 0 34 66 31 847.3 0 31 67 39 847.3 0 39 68 34 847.3 0 34 69 30 847.3 0 30 70 20 847.3 0 20 71 16 847.3 0 16 72 22 847.3 0 22 73 30 847.3 0 30 74 29 847.3 0 29 75 47 847.3 0 47 76 43 847.3 0 43 77 46 847.3 0 46 78 29 847.3 0 29 79 42 847.3 0 42 80 42 847.3 0 42 81 49 847.3 0 49 82 36 847.3 0 36 83 55 847.3 0 55 84 51 847.3 0 51 85 40 847.3 0 40 86 36 847.3 0 36 87 49 847.3 0 49 88 53 847.3 0 53 89 72 847.3 0 72 90 64 847.3 0 64 91 73 847.3 0 73 92 81 847.3 0 81 93 59 847.3 0 59 94 70 847.3 0 70 95 54 847.3 0 54 96 72 847.3 0 72 97 69 847.3 0 69 98 53 847.3 0 53 99 66 847.3 0 66 100 55 847.3 0 55 101 73 847.3 0 73 102 58 847.3 0 58 103 71 847.3 0 71 104 79 847.3 0 79 105 75 847.3 0 75 106 638 847.3 0 638 107 1036 847.3 0 1036 108 859 847.3 0 859 109 72 847.3 0 72 110 64 847.3 0 64 111 59 847.3 0 59 112 62 847.3 0 62 113 62 847.3 0 62 114 638 847.3 0 638 115 466 847.3 0 466 116 352 847.3 0 352 117 41 847.3 0 41 118 39 847.3 0 39 119 57 847.3 0 57 120 53 847.3 0 53 121 49 847.3 0 49 122 41 847.3 0 41 123 71 847.3 0 71 124 67 847.3 0 67 125 58 847.3 0 58 126 63 847.3 0 63 127 81 847.3 0 81 128 193 847.3 0 193 129 189 847.3 0 189 130 81 847.3 0 81 131 51 847.3 0 51 132 71 847.3 0 71 133 114 847.3 0 114 134 186 847.3 0 186 135 206 847.3 0 206 136 400 847.3 0 400 137 204 847.3 0 204 138 195 847.3 0 195 139 201 847.3 0 201 140 407 847.3 0 407 141 464 847.3 0 464 142 115 847.3 0 115 143 87 847.3 0 87 144 93 847.3 0 93 145 85 847.3 0 85 146 91 847.3 0 91 147 112 847.3 0 112 148 2274 847.3 0 2274 149 1134 847.3 0 1134 150 1344 847.3 0 1344 151 49 847.3 0 49 152 50 847.3 0 50 153 49 847.3 0 49 154 51 847.3 0 51 155 126 847.3 0 126 156 302 847.3 0 302 157 185 847.3 0 185 158 74 847.3 0 74 159 52 847.3 0 52 160 44 847.3 0 44 161 68 847.3 0 68 162 160 847.3 0 160 163 235 847.3 0 235 164 417 847.3 0 417 165 373 847.3 0 373 166 50 847.3 0 50 167 49 847.3 0 49 168 58 847.3 0 58 169 45 847.3 0 45 170 32 847.3 0 32 171 25 847.3 0 25 172 32 847.3 0 32 173 34 847.3 0 34 174 34 847.3 0 34 175 35 847.3 0 35 176 53 847.3 0 53 177 38 847.3 0 38 178 59 847.3 0 59 179 72 847.3 0 72 180 134 847.3 0 134 181 121 847.3 0 121 182 104 847.3 0 104 183 108 847.3 0 108 184 45 847.3 0 45 185 61 847.3 0 61 186 60 847.3 0 60 187 45 847.3 0 45 188 40 847.3 0 40 189 58 847.3 0 58 190 61 847.3 0 61 191 57 847.3 0 57 192 73 847.3 0 73 193 58 847.3 0 58 194 46 847.3 0 46 195 45 847.3 0 45 196 45 847.3 0 45 197 41 847.3 0 41 198 57 847.3 0 57 199 58 847.3 0 58 200 57 847.3 0 57 201 58 847.3 0 58 202 68 847.3 0 68 203 143 847.3 0 143 204 137 847.3 0 137 205 48 847.3 0 48 206 47 847.3 0 47 207 35 847.3 0 35 208 42 847.3 0 42 209 87 847.3 0 87 210 47 847.3 0 47 211 38 847.3 0 38 212 42 847.3 0 42 213 54 847.3 0 54 214 82 847.3 0 82 215 77 847.3 0 77 216 61 847.3 0 61 217 67 847.3 0 67 218 42 847.3 0 42 219 38 847.3 0 38 220 50 847.3 0 50 221 46 847.3 0 46 222 36 847.3 0 36 223 63 847.3 0 63 224 61 847.3 0 61 225 68 847.3 0 68 226 59 847.3 0 59 227 45 847.3 0 45 228 65 847.3 0 65 229 56 847.3 0 56 230 48 847.3 0 48 231 63 847.3 0 63 232 83 847.3 0 83 233 119 847.3 0 119 234 105 847.3 0 105 235 64 847.3 0 64 236 87 847.3 0 87 237 57 847.3 0 57 238 75 847.3 0 75 239 50 847.3 0 50 240 54 847.3 0 54 241 50 847.3 0 50 242 80 847.3 0 80 243 68 847.3 0 68 244 85 847.3 0 85 245 58 847.3 0 58 246 60 847.3 0 60 247 63 847.3 0 63 248 74 847.3 0 74 249 41 847.3 0 41 250 76 847.3 0 76 251 69 847.3 0 69 252 80 847.3 0 80 253 126 847.3 0 126 254 88 847.3 0 88 255 80 847.3 0 80 256 67 847.3 0 67 257 67 847.3 0 67 258 64 847.3 0 64 259 69 847.3 0 69 260 89 847.3 0 89 261 140 847.3 0 140 262 126 847.3 0 126 263 77 847.3 0 77 264 76 847.3 0 76 265 73 847.3 0 73 266 69 847.3 0 69 267 73 847.3 0 73 268 79 847.3 0 79 269 87 847.3 0 87 270 137 847.3 0 137 271 590 847.3 0 590 272 402 847.3 0 402 273 179 847.3 0 179 274 227 847.3 0 227 275 370 847.3 0 370 276 68 847.3 0 68 277 91 847.3 0 91 278 107 847.3 0 107 279 104 847.3 0 104 280 91 847.3 0 91 281 81 847.3 0 81 282 69 847.3 0 69 283 95 847.3 0 95 284 91 847.3 0 91 285 102 847.3 0 102 286 71 847.3 0 71 287 75 847.3 0 75 288 70 847.3 0 70 289 66 847.3 0 66 290 85 847.3 0 85 291 124 847.3 0 124 292 124 847.3 0 124 293 94 847.3 0 94 294 159 847.3 0 159 295 302 847.3 0 302 296 381 847.3 0 381 297 395 847.3 0 395 298 227 847.3 0 227 299 58 847.3 0 58 300 79 847.3 0 79 301 55 847.3 0 55 Finished in 19.84 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 250,277 Reads with adapters: 118,638 (47.4%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 118,612 (47.4%) Total basepairs processed: 70,751,622 bp Total written (filtered): 32,051,412 bp (45.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 118638 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 1 3 18 52 0.0 3 0 0 50 1 1 19 1192 0.0 3 0 1168 19 1 4 20 114885 0.0 4 112919 1893 68 5 21 298 0.0 4 71 221 6 22 36 0.0 4 13 2 21 23 67 0.0 4 1 0 0 56 10 24 3 0.0 4 2 0 0 0 1 25 1 0.0 4 1 27 2 0.0 4 2 43 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 7 0.0 4 0 0 0 0 7 126 14 0.0 4 0 0 0 0 14 127 2027 0.0 4 0 0 0 0 2027 128 5 0.0 4 0 0 0 0 5 130 3 0.0 4 0 0 0 0 3 138 1 0.0 4 1 139 4 0.0 4 0 0 0 0 4 153 2 0.0 4 0 0 0 0 2 188 1 0.0 4 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 1 237 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 269 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 1 292 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.33 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 118,612 Reads with adapters: 23,556 (19.9%) Reads written (passing filters): 118,612 (100.0%) Total basepairs processed: 32,051,412 bp Total written (filtered): 31,824,482 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 23556 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 1.6% G: 96.8% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 1853.3 0 27 4 20 463.3 0 20 5 10 115.8 0 10 6 4 29.0 0 4 7 1 7.2 0 1 8 2 1.8 0 2 9 17586 0.5 0 375 17211 10 4929 0.1 1 150 4779 11 905 0.0 1 6 899 12 22 0.0 1 0 22 13 4 0.0 1 0 4 18 1 0.0 1 0 1 108 1 0.0 1 0 1 150 4 0.0 1 0 4 151 1 0.0 1 0 1 152 1 0.0 1 0 1 153 1 0.0 1 0 1 169 3 0.0 1 3 189 1 0.0 1 0 1 191 1 0.0 1 0 1 208 21 0.0 1 6 15 210 1 0.0 1 0 1 215 8 0.0 1 8 235 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.