This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 163.99 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 283,442 (8.2%) Reads that were too short: 85,866 (2.5%) Reads written (passing filters): 197,576 (5.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 55,347,051 bp (5.3%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 283442 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3575 847.3 0 3575 7 78742 847.3 0 78742 8 4192 847.3 0 4192 9 89896 847.3 0 89896 10 101 847.3 0 101 11 37 847.3 0 37 12 36 847.3 0 36 13 30 847.3 0 30 14 35 847.3 0 35 15 32 847.3 0 32 16 98 847.3 0 98 17 199 847.3 0 199 18 214 847.3 0 214 19 36 847.3 0 36 20 33 847.3 0 33 21 39 847.3 0 39 22 38 847.3 0 38 23 31 847.3 0 31 24 28 847.3 0 28 25 84 847.3 0 84 26 33 847.3 0 33 27 29 847.3 0 29 28 21 847.3 0 21 29 31 847.3 0 31 30 37 847.3 0 37 31 33 847.3 0 33 32 34 847.3 0 34 33 32 847.3 0 32 34 32 847.3 0 32 35 32 847.3 0 32 36 24 847.3 0 24 37 39 847.3 0 39 38 43 847.3 0 43 39 21 847.3 0 21 40 24 847.3 0 24 41 26 847.3 0 26 42 32 847.3 0 32 43 33 847.3 0 33 44 42 847.3 0 42 45 30 847.3 0 30 46 26 847.3 0 26 47 39 847.3 0 39 48 43 847.3 0 43 49 37 847.3 0 37 50 120 847.3 0 120 51 247 847.3 0 247 52 46 847.3 0 46 53 54 847.3 0 54 54 61 847.3 0 61 55 50 847.3 0 50 56 55 847.3 0 55 57 50 847.3 0 50 58 29 847.3 0 29 59 41 847.3 0 41 60 37 847.3 0 37 61 65 847.3 0 65 62 57 847.3 0 57 63 53 847.3 0 53 64 43 847.3 0 43 65 60 847.3 0 60 66 107 847.3 0 107 67 1500 847.3 0 1500 68 788 847.3 0 788 69 234 847.3 0 234 70 41 847.3 0 41 71 63 847.3 0 63 72 59 847.3 0 59 73 37 847.3 0 37 74 64 847.3 0 64 75 28 847.3 0 28 76 39 847.3 0 39 77 30 847.3 0 30 78 33 847.3 0 33 79 29 847.3 0 29 80 31 847.3 0 31 81 64 847.3 0 64 82 32 847.3 0 32 83 61 847.3 0 61 84 82 847.3 0 82 85 87 847.3 0 87 86 102 847.3 0 102 87 101 847.3 0 101 88 44 847.3 0 44 89 39 847.3 0 39 90 223 847.3 0 223 91 58 847.3 0 58 92 42 847.3 0 42 93 61 847.3 0 61 94 93 847.3 0 93 95 53 847.3 0 53 96 85 847.3 0 85 97 46 847.3 0 46 98 74 847.3 0 74 99 88 847.3 0 88 100 61 847.3 0 61 101 24 847.3 0 24 102 34 847.3 0 34 103 26 847.3 0 26 104 30 847.3 0 30 105 38 847.3 0 38 106 38 847.3 0 38 107 42 847.3 0 42 108 33 847.3 0 33 109 28 847.3 0 28 110 31 847.3 0 31 111 40 847.3 0 40 112 37 847.3 0 37 113 44 847.3 0 44 114 44 847.3 0 44 115 33 847.3 0 33 116 34 847.3 0 34 117 39 847.3 0 39 118 91 847.3 0 91 119 100 847.3 0 100 120 107 847.3 0 107 121 127 847.3 0 127 122 119 847.3 0 119 123 82 847.3 0 82 124 27 847.3 0 27 125 39 847.3 0 39 126 70 847.3 0 70 127 156 847.3 0 156 128 83 847.3 0 83 129 170 847.3 0 170 130 69 847.3 0 69 131 55 847.3 0 55 132 150 847.3 0 150 133 362 847.3 0 362 134 69 847.3 0 69 135 44 847.3 0 44 136 32 847.3 0 32 137 42 847.3 0 42 138 42 847.3 0 42 139 56 847.3 0 56 140 41 847.3 0 41 141 49 847.3 0 49 142 41 847.3 0 41 143 44 847.3 0 44 144 51 847.3 0 51 145 60 847.3 0 60 146 74 847.3 0 74 147 90 847.3 0 90 148 85 847.3 0 85 149 77 847.3 0 77 150 73 847.3 0 73 151 78 847.3 0 78 152 446 847.3 0 446 153 493 847.3 0 493 154 211 847.3 0 211 155 114 847.3 0 114 156 56 847.3 0 56 157 70 847.3 0 70 158 133 847.3 0 133 159 257 847.3 0 257 160 79 847.3 0 79 161 73 847.3 0 73 162 82 847.3 0 82 163 297 847.3 0 297 164 514 847.3 0 514 165 623 847.3 0 623 166 354 847.3 0 354 167 135 847.3 0 135 168 57 847.3 0 57 169 62 847.3 0 62 170 54 847.3 0 54 171 40 847.3 0 40 172 56 847.3 0 56 173 120 847.3 0 120 174 112 847.3 0 112 175 95 847.3 0 95 176 85 847.3 0 85 177 82 847.3 0 82 178 125 847.3 0 125 179 145 847.3 0 145 180 238 847.3 0 238 181 145 847.3 0 145 182 121 847.3 0 121 183 463 847.3 0 463 184 471 847.3 0 471 185 358 847.3 0 358 186 322 847.3 0 322 187 178 847.3 0 178 188 95 847.3 0 95 189 112 847.3 0 112 190 73 847.3 0 73 191 95 847.3 0 95 192 304 847.3 0 304 193 364 847.3 0 364 194 357 847.3 0 357 195 550 847.3 0 550 196 69 847.3 0 69 197 58 847.3 0 58 198 218 847.3 0 218 199 278 847.3 0 278 200 175 847.3 0 175 201 216 847.3 0 216 202 358 847.3 0 358 203 154 847.3 0 154 204 146 847.3 0 146 205 197 847.3 0 197 206 235 847.3 0 235 207 370 847.3 0 370 208 246 847.3 0 246 209 224 847.3 0 224 210 102 847.3 0 102 211 115 847.3 0 115 212 227 847.3 0 227 213 225 847.3 0 225 214 435 847.3 0 435 215 529 847.3 0 529 216 617 847.3 0 617 217 1107 847.3 0 1107 218 1317 847.3 0 1317 219 8812 847.3 0 8812 220 13891 847.3 0 13891 221 40497 847.3 0 40497 222 643 847.3 0 643 223 71 847.3 0 71 224 80 847.3 0 80 225 64 847.3 0 64 226 55 847.3 0 55 227 64 847.3 0 64 228 80 847.3 0 80 229 67 847.3 0 67 230 106 847.3 0 106 231 76 847.3 0 76 232 71 847.3 0 71 233 90 847.3 0 90 234 121 847.3 0 121 235 123 847.3 0 123 236 123 847.3 0 123 237 176 847.3 0 176 238 147 847.3 0 147 239 135 847.3 0 135 240 142 847.3 0 142 241 95 847.3 0 95 242 72 847.3 0 72 243 94 847.3 0 94 244 86 847.3 0 86 245 72 847.3 0 72 246 114 847.3 0 114 247 193 847.3 0 193 248 696 847.3 0 696 249 997 847.3 0 997 250 797 847.3 0 797 251 370 847.3 0 370 252 229 847.3 0 229 253 204 847.3 0 204 254 146 847.3 0 146 255 107 847.3 0 107 256 105 847.3 0 105 257 101 847.3 0 101 258 99 847.3 0 99 259 81 847.3 0 81 260 89 847.3 0 89 261 51 847.3 0 51 262 91 847.3 0 91 263 62 847.3 0 62 264 58 847.3 0 58 265 82 847.3 0 82 266 117 847.3 0 117 267 153 847.3 0 153 268 123 847.3 0 123 269 118 847.3 0 118 270 108 847.3 0 108 271 109 847.3 0 109 272 203 847.3 0 203 273 242 847.3 0 242 274 126 847.3 0 126 275 108 847.3 0 108 276 90 847.3 0 90 277 108 847.3 0 108 278 178 847.3 0 178 279 632 847.3 0 632 280 1053 847.3 0 1053 281 1298 847.3 0 1298 282 625 847.3 0 625 283 130 847.3 0 130 284 186 847.3 0 186 285 129 847.3 0 129 286 120 847.3 0 120 287 157 847.3 0 157 288 151 847.3 0 151 289 112 847.3 0 112 290 103 847.3 0 103 291 104 847.3 0 104 292 242 847.3 0 242 293 251 847.3 0 251 294 265 847.3 0 265 295 156 847.3 0 156 296 95 847.3 0 95 297 144 847.3 0 144 298 123 847.3 0 123 299 175 847.3 0 175 300 263 847.3 0 263 301 240 847.3 0 240 Finished in 14.35 s (73 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 197,576 Reads with adapters: 95,300 (48.2%) Reads that were too short: 166 (0.1%) Reads written (passing filters): 95,134 (48.2%) Total basepairs processed: 55,347,051 bp Total written (filtered): 26,139,656 bp (47.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 95300 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 41 0.0 3 0 0 26 15 16 773 0.0 3 0 685 62 26 17 93421 0.0 3 88339 4226 689 167 18 304 0.0 3 15 219 23 47 19 129 0.0 3 0 0 82 47 20 268 0.0 3 1 0 0 267 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 1 0 1 26 6 0.0 3 0 0 1 5 27 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 6 0.0 3 0 0 0 6 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 4 0.0 3 0 0 0 4 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 5 0.0 3 0 0 0 5 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 5 0.0 3 0 0 0 5 55 8 0.0 3 0 0 0 8 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 1 61 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 1 90 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 98 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 4 0.0 3 0 0 0 4 106 4 0.0 3 0 0 0 4 107 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 109 53 0.0 3 0 0 0 53 110 2 0.0 3 0 0 2 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 63 0.0 3 0 0 0 63 117 1 0.0 3 0 0 0 1 119 8 0.0 3 0 0 0 8 120 13 0.0 3 0 0 0 13 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 3 0.0 3 0 0 0 3 156 3 0.0 3 0 0 1 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 1 164 2 0.0 3 0 0 1 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 7 0.0 3 0 0 1 6 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 234 3 0.0 3 0 0 0 3 235 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 5 0.0 3 0 0 0 5 281 10 0.0 3 0 0 1 9 282 9 0.0 3 0 0 0 9 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.85 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 95,134 Reads with adapters: 9,640 (10.1%) Reads written (passing filters): 95,134 (100.0%) Total basepairs processed: 26,139,656 bp Total written (filtered): 26,110,661 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9640 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.2% C: 10.4% G: 10.2% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9572 1486.5 0 9572 4 63 371.6 0 63 5 3 92.9 0 3 6 2 23.2 0 2