This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 170.67 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 704,979 (20.3%) Reads that were too short: 37,589 (1.1%) Reads written (passing filters): 667,390 (19.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 154,425,545 bp (14.8%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 704979 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1969 847.3 0 1969 7 4389 847.3 0 4389 8 111405 847.3 0 111405 9 90086 847.3 0 90086 10 202 847.3 0 202 11 36 847.3 0 36 12 35 847.3 0 35 13 24 847.3 0 24 14 24 847.3 0 24 15 24 847.3 0 24 16 37 847.3 0 37 17 43 847.3 0 43 18 25 847.3 0 25 19 36 847.3 0 36 20 33 847.3 0 33 21 37 847.3 0 37 22 53 847.3 0 53 23 77 847.3 0 77 24 71 847.3 0 71 25 39 847.3 0 39 26 75 847.3 0 75 27 66 847.3 0 66 28 29 847.3 0 29 29 32 847.3 0 32 30 35 847.3 0 35 31 50 847.3 0 50 32 70 847.3 0 70 33 28 847.3 0 28 34 28 847.3 0 28 35 38 847.3 0 38 36 24 847.3 0 24 37 39 847.3 0 39 38 118 847.3 0 118 39 136 847.3 0 136 40 145 847.3 0 145 41 246 847.3 0 246 42 81 847.3 0 81 43 33 847.3 0 33 44 22 847.3 0 22 45 21 847.3 0 21 46 35 847.3 0 35 47 38 847.3 0 38 48 58 847.3 0 58 49 73 847.3 0 73 50 79 847.3 0 79 51 60 847.3 0 60 52 87 847.3 0 87 53 118 847.3 0 118 54 139 847.3 0 139 55 35 847.3 0 35 56 43 847.3 0 43 57 105 847.3 0 105 58 87 847.3 0 87 59 59 847.3 0 59 60 113 847.3 0 113 61 57 847.3 0 57 62 116 847.3 0 116 63 113 847.3 0 113 64 41 847.3 0 41 65 80 847.3 0 80 66 151 847.3 0 151 67 111 847.3 0 111 68 334 847.3 0 334 69 127 847.3 0 127 70 109 847.3 0 109 71 83 847.3 0 83 72 281 847.3 0 281 73 190 847.3 0 190 74 280 847.3 0 280 75 456 847.3 0 456 76 676 847.3 0 676 77 858 847.3 0 858 78 1768 847.3 0 1768 79 3382 847.3 0 3382 80 844 847.3 0 844 81 1223 847.3 0 1223 82 2154 847.3 0 2154 83 1220 847.3 0 1220 84 1030 847.3 0 1030 85 2513 847.3 0 2513 86 2069 847.3 0 2069 87 2101 847.3 0 2101 88 2216 847.3 0 2216 89 3281 847.3 0 3281 90 5414 847.3 0 5414 91 9195 847.3 0 9195 92 192608 847.3 0 192608 93 111411 847.3 0 111411 94 82109 847.3 0 82109 95 790 847.3 0 790 96 36 847.3 0 36 97 63 847.3 0 63 98 107 847.3 0 107 99 46 847.3 0 46 100 37 847.3 0 37 101 18 847.3 0 18 102 21 847.3 0 21 103 21 847.3 0 21 104 20 847.3 0 20 105 16 847.3 0 16 106 20 847.3 0 20 107 34 847.3 0 34 108 32 847.3 0 32 109 61 847.3 0 61 110 150 847.3 0 150 111 86 847.3 0 86 112 57 847.3 0 57 113 24 847.3 0 24 114 56 847.3 0 56 115 38 847.3 0 38 116 47 847.3 0 47 117 35 847.3 0 35 118 50 847.3 0 50 119 222 847.3 0 222 120 21 847.3 0 21 121 17 847.3 0 17 122 19 847.3 0 19 123 10 847.3 0 10 124 12 847.3 0 12 125 34 847.3 0 34 126 21 847.3 0 21 127 17 847.3 0 17 128 50 847.3 0 50 129 132 847.3 0 132 130 40 847.3 0 40 131 54 847.3 0 54 132 24 847.3 0 24 133 54 847.3 0 54 134 312 847.3 0 312 135 26 847.3 0 26 136 33 847.3 0 33 137 44 847.3 0 44 138 53 847.3 0 53 139 30 847.3 0 30 140 25 847.3 0 25 141 18 847.3 0 18 142 25 847.3 0 25 143 44 847.3 0 44 144 45 847.3 0 45 145 33 847.3 0 33 146 19 847.3 0 19 147 12 847.3 0 12 148 24 847.3 0 24 149 22 847.3 0 22 150 22 847.3 0 22 151 28 847.3 0 28 152 26 847.3 0 26 153 21 847.3 0 21 154 30 847.3 0 30 155 27 847.3 0 27 156 25 847.3 0 25 157 34 847.3 0 34 158 37 847.3 0 37 159 33 847.3 0 33 160 36 847.3 0 36 161 28 847.3 0 28 162 23 847.3 0 23 163 25 847.3 0 25 164 26 847.3 0 26 165 34 847.3 0 34 166 27 847.3 0 27 167 24 847.3 0 24 168 19 847.3 0 19 169 30 847.3 0 30 170 18 847.3 0 18 171 36 847.3 0 36 172 33 847.3 0 33 173 33 847.3 0 33 174 44 847.3 0 44 175 31 847.3 0 31 176 42 847.3 0 42 177 78 847.3 0 78 178 302 847.3 0 302 179 728 847.3 0 728 180 765 847.3 0 765 181 170 847.3 0 170 182 35 847.3 0 35 183 48 847.3 0 48 184 66 847.3 0 66 185 77 847.3 0 77 186 116 847.3 0 116 187 191 847.3 0 191 188 348 847.3 0 348 189 111 847.3 0 111 190 203 847.3 0 203 191 213 847.3 0 213 192 204 847.3 0 204 193 123 847.3 0 123 194 205 847.3 0 205 195 211 847.3 0 211 196 272 847.3 0 272 197 281 847.3 0 281 198 367 847.3 0 367 199 480 847.3 0 480 200 954 847.3 0 954 201 17002 847.3 0 17002 202 12412 847.3 0 12412 203 4945 847.3 0 4945 204 372 847.3 0 372 205 1287 847.3 0 1287 206 1381 847.3 0 1381 207 1269 847.3 0 1269 208 651 847.3 0 651 209 427 847.3 0 427 210 162 847.3 0 162 211 61 847.3 0 61 212 70 847.3 0 70 213 76 847.3 0 76 214 41 847.3 0 41 215 84 847.3 0 84 216 122 847.3 0 122 217 195 847.3 0 195 218 80 847.3 0 80 219 201 847.3 0 201 220 85 847.3 0 85 221 273 847.3 0 273 222 154 847.3 0 154 223 205 847.3 0 205 224 152 847.3 0 152 225 288 847.3 0 288 226 984 847.3 0 984 227 964 847.3 0 964 228 751 847.3 0 751 229 123 847.3 0 123 230 40 847.3 0 40 231 42 847.3 0 42 232 48 847.3 0 48 233 37 847.3 0 37 234 29 847.3 0 29 235 29 847.3 0 29 236 93 847.3 0 93 237 65 847.3 0 65 238 58 847.3 0 58 239 24 847.3 0 24 240 16 847.3 0 16 241 14 847.3 0 14 242 23 847.3 0 23 243 36 847.3 0 36 244 36 847.3 0 36 245 43 847.3 0 43 246 50 847.3 0 50 247 36 847.3 0 36 248 46 847.3 0 46 249 49 847.3 0 49 250 34 847.3 0 34 251 29 847.3 0 29 252 29 847.3 0 29 253 36 847.3 0 36 254 51 847.3 0 51 255 53 847.3 0 53 256 60 847.3 0 60 257 66 847.3 0 66 258 74 847.3 0 74 259 267 847.3 0 267 260 633 847.3 0 633 261 1043 847.3 0 1043 262 733 847.3 0 733 263 419 847.3 0 419 264 265 847.3 0 265 265 67 847.3 0 67 266 40 847.3 0 40 267 33 847.3 0 33 268 28 847.3 0 28 269 50 847.3 0 50 270 196 847.3 0 196 271 225 847.3 0 225 272 139 847.3 0 139 273 116 847.3 0 116 274 29 847.3 0 29 275 52 847.3 0 52 276 39 847.3 0 39 277 92 847.3 0 92 278 69 847.3 0 69 279 62 847.3 0 62 280 81 847.3 0 81 281 137 847.3 0 137 282 132 847.3 0 132 283 90 847.3 0 90 284 100 847.3 0 100 285 535 847.3 0 535 286 392 847.3 0 392 287 357 847.3 0 357 288 85 847.3 0 85 289 24 847.3 0 24 290 38 847.3 0 38 291 38 847.3 0 38 292 24 847.3 0 24 293 28 847.3 0 28 294 53 847.3 0 53 295 96 847.3 0 96 296 148 847.3 0 148 297 989 847.3 0 989 298 289 847.3 0 289 299 223 847.3 0 223 300 45 847.3 0 45 301 27 847.3 0 27 Finished in 41.07 s (62 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 667,390 Reads with adapters: 118,424 (17.7%) Reads that were too short: 606 (0.1%) Reads written (passing filters): 117,818 (17.7%) Total basepairs processed: 154,425,545 bp Total written (filtered): 31,453,431 bp (20.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 118424 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 22 0.0 2 0 0 0 22 15 58 0.0 3 0 0 37 21 16 874 0.0 3 0 705 105 64 17 109531 0.0 3 103819 4754 779 179 18 290 0.0 3 25 184 35 46 19 202 0.0 3 2 2 115 83 20 372 0.0 3 2 3 0 367 22 1 0.0 3 0 0 0 1 24 2 0.0 3 2 25 5 0.0 3 0 0 0 5 27 3 0.0 3 2 0 0 1 29 1 0.0 3 0 0 0 1 30 9 0.0 3 0 0 0 9 31 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 2 0.0 3 1 0 0 1 42 3 0.0 3 1 0 0 2 45 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 19 0.0 3 0 0 0 19 55 6 0.0 3 0 0 0 6 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 84 0.0 3 0 0 0 84 75 12 0.0 3 0 0 0 12 76 5 0.0 3 0 0 0 5 77 19 0.0 3 0 0 0 19 78 1 0.0 3 0 0 0 1 79 34 0.0 3 0 0 1 33 80 12 0.0 3 0 0 0 12 81 3 0.0 3 0 0 0 3 82 4 0.0 3 0 0 0 4 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 1 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 5 0.0 3 0 0 0 5 95 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 32 0.0 3 0 0 0 32 99 4628 0.0 3 0 0 31 4597 100 1059 0.0 3 0 0 9 1050 101 10 0.0 3 0 0 0 10 102 336 0.0 3 0 0 9 327 103 132 0.0 3 0 0 5 127 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 4 0.0 3 0 0 0 4 124 11 0.0 3 0 0 0 11 125 15 0.0 3 0 0 2 13 126 1 0.0 3 0 0 0 1 127 2 0.0 3 1 0 0 1 131 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 138 2 0.0 3 1 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 2 0.0 3 1 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 1 147 3 0.0 3 0 1 0 2 149 4 0.0 3 0 0 0 4 150 54 0.0 3 0 0 3 51 151 15 0.0 3 0 0 0 15 152 5 0.0 3 0 0 0 5 153 11 0.0 3 0 0 1 10 154 4 0.0 3 0 0 0 4 155 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 3 0.0 3 0 0 0 3 159 15 0.0 3 0 0 0 15 160 17 0.0 3 0 0 0 17 161 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 1 3 164 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 172 3 0.0 3 0 0 0 3 173 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 175 3 0.0 3 0 0 0 3 176 3 0.0 3 0 0 0 3 177 6 0.0 3 0 0 0 6 178 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 4 0.0 3 0 0 1 3 188 2 0.0 3 0 0 0 2 190 3 0.0 3 0 0 0 3 191 23 0.0 3 0 0 0 23 192 9 0.0 3 0 0 0 9 194 6 0.0 3 0 0 0 6 195 1 0.0 3 0 0 1 199 11 0.0 3 0 0 0 11 200 199 0.0 3 0 0 2 197 201 31 0.0 3 0 0 0 31 202 17 0.0 3 0 0 0 17 203 4 0.0 3 0 0 0 4 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 4 0.0 3 0 0 0 4 208 3 0.0 3 0 0 0 3 209 4 0.0 3 0 0 0 4 211 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 1 278 3 0.0 3 0 0 0 3 279 5 0.0 3 0 0 0 5 280 11 0.0 3 0 0 0 11 281 6 0.0 3 0 0 1 5 282 6 0.0 3 0 0 0 6 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.25 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 117,818 Reads with adapters: 3,667 (3.1%) Reads written (passing filters): 117,818 (100.0%) Total basepairs processed: 31,453,431 bp Total written (filtered): 31,442,156 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3667 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.6% C: 11.9% G: 9.8% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3413 1840.9 0 3413 4 236 460.2 0 236 5 16 115.1 0 16 6 2 28.8 0 2