This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 170.55 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 552,215 (15.9%) Reads that were too short: 16,661 (0.5%) Reads written (passing filters): 535,554 (15.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 128,937,937 bp (12.3%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 552215 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2573 847.3 0 2573 7 91662 847.3 0 91662 8 66935 847.3 0 66935 9 109 847.3 0 109 10 183 847.3 0 183 11 86 847.3 0 86 12 21 847.3 0 21 13 64 847.3 0 64 14 17 847.3 0 17 15 15 847.3 0 15 16 10 847.3 0 10 17 9 847.3 0 9 18 9 847.3 0 9 19 12 847.3 0 12 20 19 847.3 0 19 21 23 847.3 0 23 22 16 847.3 0 16 23 19 847.3 0 19 24 5 847.3 0 5 25 15 847.3 0 15 26 7 847.3 0 7 27 42 847.3 0 42 28 29 847.3 0 29 29 37 847.3 0 37 30 23 847.3 0 23 31 20 847.3 0 20 32 13 847.3 0 13 33 16 847.3 0 16 34 21 847.3 0 21 35 27 847.3 0 27 36 29 847.3 0 29 37 13 847.3 0 13 38 14 847.3 0 14 39 52 847.3 0 52 40 31 847.3 0 31 41 27 847.3 0 27 42 79 847.3 0 79 43 104 847.3 0 104 44 66 847.3 0 66 45 148 847.3 0 148 46 199 847.3 0 199 47 95 847.3 0 95 48 67 847.3 0 67 49 226 847.3 0 226 50 57 847.3 0 57 51 104 847.3 0 104 52 936 847.3 0 936 53 244 847.3 0 244 54 88 847.3 0 88 55 88 847.3 0 88 56 40 847.3 0 40 57 45 847.3 0 45 58 27 847.3 0 27 59 287 847.3 0 287 60 225 847.3 0 225 61 516 847.3 0 516 62 1261 847.3 0 1261 63 265 847.3 0 265 64 549 847.3 0 549 65 1020 847.3 0 1020 66 1335 847.3 0 1335 67 2139 847.3 0 2139 68 906 847.3 0 906 69 719 847.3 0 719 70 211 847.3 0 211 71 341 847.3 0 341 72 1033 847.3 0 1033 73 1114 847.3 0 1114 74 1660 847.3 0 1660 75 2386 847.3 0 2386 76 2600 847.3 0 2600 77 5207 847.3 0 5207 78 7759 847.3 0 7759 79 86476 847.3 0 86476 80 60085 847.3 0 60085 81 169643 847.3 0 169643 82 995 847.3 0 995 83 564 847.3 0 564 84 153 847.3 0 153 85 123 847.3 0 123 86 74 847.3 0 74 87 98 847.3 0 98 88 142 847.3 0 142 89 41 847.3 0 41 90 26 847.3 0 26 91 23 847.3 0 23 92 32 847.3 0 32 93 75 847.3 0 75 94 62 847.3 0 62 95 32 847.3 0 32 96 26 847.3 0 26 97 20 847.3 0 20 98 11 847.3 0 11 99 28 847.3 0 28 100 35 847.3 0 35 101 37 847.3 0 37 102 37 847.3 0 37 103 68 847.3 0 68 104 38 847.3 0 38 105 37 847.3 0 37 106 32 847.3 0 32 107 21 847.3 0 21 108 30 847.3 0 30 109 46 847.3 0 46 110 62 847.3 0 62 111 64 847.3 0 64 112 60 847.3 0 60 113 73 847.3 0 73 114 90 847.3 0 90 115 117 847.3 0 117 116 689 847.3 0 689 117 642 847.3 0 642 118 522 847.3 0 522 119 178 847.3 0 178 120 115 847.3 0 115 121 139 847.3 0 139 122 125 847.3 0 125 123 207 847.3 0 207 124 322 847.3 0 322 125 127 847.3 0 127 126 183 847.3 0 183 127 99 847.3 0 99 128 112 847.3 0 112 129 108 847.3 0 108 130 296 847.3 0 296 131 394 847.3 0 394 132 326 847.3 0 326 133 109 847.3 0 109 134 91 847.3 0 91 135 117 847.3 0 117 136 160 847.3 0 160 137 139 847.3 0 139 138 168 847.3 0 168 139 249 847.3 0 249 140 179 847.3 0 179 141 274 847.3 0 274 142 297 847.3 0 297 143 142 847.3 0 142 144 162 847.3 0 162 145 233 847.3 0 233 146 356 847.3 0 356 147 282 847.3 0 282 148 204 847.3 0 204 149 305 847.3 0 305 150 3164 847.3 0 3164 151 2075 847.3 0 2075 152 1273 847.3 0 1273 153 134 847.3 0 134 154 81 847.3 0 81 155 82 847.3 0 82 156 86 847.3 0 86 157 101 847.3 0 101 158 91 847.3 0 91 159 81 847.3 0 81 160 76 847.3 0 76 161 79 847.3 0 79 162 81 847.3 0 81 163 74 847.3 0 74 164 78 847.3 0 78 165 87 847.3 0 87 166 93 847.3 0 93 167 136 847.3 0 136 168 114 847.3 0 114 169 137 847.3 0 137 170 128 847.3 0 128 171 290 847.3 0 290 172 282 847.3 0 282 173 179 847.3 0 179 174 159 847.3 0 159 175 69 847.3 0 69 176 70 847.3 0 70 177 74 847.3 0 74 178 65 847.3 0 65 179 85 847.3 0 85 180 78 847.3 0 78 181 72 847.3 0 72 182 65 847.3 0 65 183 78 847.3 0 78 184 103 847.3 0 103 185 57 847.3 0 57 186 183 847.3 0 183 187 118 847.3 0 118 188 92 847.3 0 92 189 98 847.3 0 98 190 70 847.3 0 70 191 87 847.3 0 87 192 93 847.3 0 93 193 85 847.3 0 85 194 89 847.3 0 89 195 100 847.3 0 100 196 76 847.3 0 76 197 102 847.3 0 102 198 86 847.3 0 86 199 107 847.3 0 107 200 127 847.3 0 127 201 88 847.3 0 88 202 83 847.3 0 83 203 69 847.3 0 69 204 99 847.3 0 99 205 102 847.3 0 102 206 74 847.3 0 74 207 72 847.3 0 72 208 76 847.3 0 76 209 70 847.3 0 70 210 84 847.3 0 84 211 102 847.3 0 102 212 94 847.3 0 94 213 115 847.3 0 115 214 87 847.3 0 87 215 100 847.3 0 100 216 104 847.3 0 104 217 137 847.3 0 137 218 288 847.3 0 288 219 305 847.3 0 305 220 179 847.3 0 179 221 137 847.3 0 137 222 151 847.3 0 151 223 198 847.3 0 198 224 223 847.3 0 223 225 246 847.3 0 246 226 1011 847.3 0 1011 227 1008 847.3 0 1008 228 1272 847.3 0 1272 229 358 847.3 0 358 230 173 847.3 0 173 231 84 847.3 0 84 232 94 847.3 0 94 233 86 847.3 0 86 234 85 847.3 0 85 235 72 847.3 0 72 236 83 847.3 0 83 237 101 847.3 0 101 238 89 847.3 0 89 239 114 847.3 0 114 240 112 847.3 0 112 241 167 847.3 0 167 242 86 847.3 0 86 243 96 847.3 0 96 244 95 847.3 0 95 245 97 847.3 0 97 246 153 847.3 0 153 247 178 847.3 0 178 248 137 847.3 0 137 249 101 847.3 0 101 250 116 847.3 0 116 251 146 847.3 0 146 252 144 847.3 0 144 253 104 847.3 0 104 254 107 847.3 0 107 255 90 847.3 0 90 256 111 847.3 0 111 257 105 847.3 0 105 258 81 847.3 0 81 259 100 847.3 0 100 260 114 847.3 0 114 261 154 847.3 0 154 262 108 847.3 0 108 263 106 847.3 0 106 264 135 847.3 0 135 265 142 847.3 0 142 266 134 847.3 0 134 267 86 847.3 0 86 268 126 847.3 0 126 269 86 847.3 0 86 270 96 847.3 0 96 271 83 847.3 0 83 272 120 847.3 0 120 273 99 847.3 0 99 274 195 847.3 0 195 275 231 847.3 0 231 276 246 847.3 0 246 277 138 847.3 0 138 278 129 847.3 0 129 279 257 847.3 0 257 280 254 847.3 0 254 281 205 847.3 0 205 282 109 847.3 0 109 283 75 847.3 0 75 284 79 847.3 0 79 285 179 847.3 0 179 286 228 847.3 0 228 287 177 847.3 0 177 288 146 847.3 0 146 289 136 847.3 0 136 290 200 847.3 0 200 291 257 847.3 0 257 292 269 847.3 0 269 293 189 847.3 0 189 294 146 847.3 0 146 295 86 847.3 0 86 296 123 847.3 0 123 297 138 847.3 0 138 298 153 847.3 0 153 299 174 847.3 0 174 300 242 847.3 0 242 301 160 847.3 0 160 Finished in 34.56 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 535,554 Reads with adapters: 81,645 (15.2%) Reads that were too short: 378 (0.1%) Reads written (passing filters): 81,267 (15.2%) Total basepairs processed: 128,937,937 bp Total written (filtered): 22,451,924 bp (17.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 81645 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 32 0.0 3 0 0 24 8 16 747 0.0 3 0 676 55 16 17 79389 0.0 3 75484 3308 492 105 18 220 0.0 3 8 167 18 27 19 139 0.0 3 41 15 48 35 20 343 0.0 3 0 1 0 342 22 1 0.0 3 1 23 4 0.0 3 0 0 1 3 24 2 0.0 3 0 0 1 1 25 5 0.0 3 1 0 1 3 26 14 0.0 3 0 0 0 14 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 31 5 0.0 3 0 0 0 5 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 41 116 0.0 3 0 0 1 115 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 18 0.0 3 0 0 0 18 46 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 48 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 7 0.0 3 0 0 1 6 56 6 0.0 3 0 0 0 6 57 19 0.0 3 0 0 0 19 58 4 0.0 3 0 0 0 4 64 3 0.0 3 0 0 1 2 66 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 75 8 0.0 3 0 0 0 8 76 21 0.0 3 0 1 0 20 78 4 0.0 3 0 0 1 3 79 4 0.0 3 0 0 0 4 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 104 3 0.0 3 1 0 0 2 106 28 0.0 3 0 0 0 28 107 8 0.0 3 0 0 1 7 108 11 0.0 3 0 0 1 10 109 14 0.0 3 0 0 1 13 110 4 0.0 3 0 0 0 4 111 6 0.0 3 0 0 0 6 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 2 0.0 3 1 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 0 3 119 2 0.0 3 1 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 4 0.0 3 0 0 0 4 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 35 0.0 3 0 0 0 35 134 4 0.0 3 0 0 0 4 135 4 0.0 3 0 0 0 4 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 1 4 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 149 14 0.0 3 0 0 0 14 150 3 0.0 3 0 0 0 3 151 3 0.0 3 0 0 0 3 152 2 0.0 3 0 0 0 2 153 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 1 1 157 2 0.0 3 0 0 0 2 158 3 0.0 3 0 0 0 3 159 2 0.0 3 0 0 1 1 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 6 0.0 3 0 0 0 6 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 172 6 0.0 3 0 0 0 6 177 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 197 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 18 0.0 3 0 0 0 18 208 33 0.0 3 0 0 0 33 209 40 0.0 3 0 0 0 40 210 45 0.0 3 0 0 0 45 211 12 0.0 3 0 0 0 12 212 2 0.0 3 0 0 0 2 213 3 0.0 3 0 0 0 3 214 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 5 0.0 3 0 0 0 5 219 5 0.0 3 0 0 0 5 220 4 0.0 3 0 0 0 4 221 5 0.0 3 0 0 0 5 222 4 0.0 3 0 0 0 4 223 4 0.0 3 0 0 0 4 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 277 3 0.0 3 0 0 0 3 279 4 0.0 3 0 0 1 3 280 3 0.0 3 0 0 0 3 281 4 0.0 3 0 0 0 4 282 6 0.0 3 0 0 0 6 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.67 s (57 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 81,267 Reads with adapters: 381 (0.5%) Reads written (passing filters): 81,267 (100.0%) Total basepairs processed: 22,451,924 bp Total written (filtered): 22,450,693 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 381 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.0% C: 24.4% G: 21.5% T: 33.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 345 1269.8 0 345 4 23 317.4 0 23 5 7 79.4 0 7 6 3 19.8 0 3 11 2 0.0 1 0 2 29 1 0.0 2 0 0 1