This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.24 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 222,156 (6.4%) Reads that were too short: 6,610 (0.2%) Reads written (passing filters): 215,546 (6.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 59,645,653 bp (5.7%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 222156 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6329 847.3 0 6329 7 70926 847.3 0 70926 8 2496 847.3 0 2496 9 79649 847.3 0 79649 10 93 847.3 0 93 11 25 847.3 0 25 12 13 847.3 0 13 13 21 847.3 0 21 14 11 847.3 0 11 15 13 847.3 0 13 16 22 847.3 0 22 17 25 847.3 0 25 18 24 847.3 0 24 19 32 847.3 0 32 20 37 847.3 0 37 21 23 847.3 0 23 22 28 847.3 0 28 23 42 847.3 0 42 24 61 847.3 0 61 25 45 847.3 0 45 26 29 847.3 0 29 27 64 847.3 0 64 28 175 847.3 0 175 29 84 847.3 0 84 30 366 847.3 0 366 31 18 847.3 0 18 32 21 847.3 0 21 33 104 847.3 0 104 34 419 847.3 0 419 35 128 847.3 0 128 36 41 847.3 0 41 37 99 847.3 0 99 38 31 847.3 0 31 39 60 847.3 0 60 40 70 847.3 0 70 41 58 847.3 0 58 42 103 847.3 0 103 43 87 847.3 0 87 44 65 847.3 0 65 45 34 847.3 0 34 46 59 847.3 0 59 47 102 847.3 0 102 48 126 847.3 0 126 49 235 847.3 0 235 50 323 847.3 0 323 51 404 847.3 0 404 52 564 847.3 0 564 53 711 847.3 0 711 54 3722 847.3 0 3722 55 16696 847.3 0 16696 56 17675 847.3 0 17675 57 88 847.3 0 88 58 31 847.3 0 31 59 21 847.3 0 21 60 31 847.3 0 31 61 13 847.3 0 13 62 15 847.3 0 15 63 11 847.3 0 11 64 16 847.3 0 16 65 21 847.3 0 21 66 8 847.3 0 8 67 26 847.3 0 26 68 24 847.3 0 24 69 32 847.3 0 32 70 25 847.3 0 25 71 13 847.3 0 13 72 15 847.3 0 15 73 30 847.3 0 30 74 17 847.3 0 17 75 11 847.3 0 11 76 21 847.3 0 21 77 41 847.3 0 41 78 35 847.3 0 35 79 29 847.3 0 29 80 28 847.3 0 28 81 43 847.3 0 43 82 29 847.3 0 29 83 33 847.3 0 33 84 31 847.3 0 31 85 34 847.3 0 34 86 30 847.3 0 30 87 34 847.3 0 34 88 28 847.3 0 28 89 20 847.3 0 20 90 35 847.3 0 35 91 36 847.3 0 36 92 56 847.3 0 56 93 209 847.3 0 209 94 1792 847.3 0 1792 95 1156 847.3 0 1156 96 192 847.3 0 192 97 23 847.3 0 23 98 20 847.3 0 20 99 21 847.3 0 21 100 12 847.3 0 12 101 12 847.3 0 12 102 15 847.3 0 15 103 27 847.3 0 27 104 28 847.3 0 28 105 25 847.3 0 25 106 14 847.3 0 14 107 25 847.3 0 25 108 32 847.3 0 32 109 102 847.3 0 102 110 272 847.3 0 272 111 404 847.3 0 404 112 21 847.3 0 21 113 12 847.3 0 12 114 26 847.3 0 26 115 30 847.3 0 30 116 37 847.3 0 37 117 48 847.3 0 48 118 50 847.3 0 50 119 61 847.3 0 61 120 163 847.3 0 163 121 1100 847.3 0 1100 122 510 847.3 0 510 123 207 847.3 0 207 124 35 847.3 0 35 125 28 847.3 0 28 126 31 847.3 0 31 127 31 847.3 0 31 128 40 847.3 0 40 129 33 847.3 0 33 130 33 847.3 0 33 131 14 847.3 0 14 132 21 847.3 0 21 133 30 847.3 0 30 134 26 847.3 0 26 135 58 847.3 0 58 136 127 847.3 0 127 137 75 847.3 0 75 138 44 847.3 0 44 139 82 847.3 0 82 140 95 847.3 0 95 141 104 847.3 0 104 142 57 847.3 0 57 143 42 847.3 0 42 144 22 847.3 0 22 145 59 847.3 0 59 146 50 847.3 0 50 147 102 847.3 0 102 148 119 847.3 0 119 149 101 847.3 0 101 150 79 847.3 0 79 151 46 847.3 0 46 152 45 847.3 0 45 153 42 847.3 0 42 154 44 847.3 0 44 155 27 847.3 0 27 156 41 847.3 0 41 157 48 847.3 0 48 158 40 847.3 0 40 159 69 847.3 0 69 160 116 847.3 0 116 161 324 847.3 0 324 162 192 847.3 0 192 163 83 847.3 0 83 164 38 847.3 0 38 165 102 847.3 0 102 166 124 847.3 0 124 167 100 847.3 0 100 168 56 847.3 0 56 169 29 847.3 0 29 170 48 847.3 0 48 171 43 847.3 0 43 172 62 847.3 0 62 173 44 847.3 0 44 174 68 847.3 0 68 175 92 847.3 0 92 176 156 847.3 0 156 177 221 847.3 0 221 178 283 847.3 0 283 179 34 847.3 0 34 180 84 847.3 0 84 181 200 847.3 0 200 182 164 847.3 0 164 183 51 847.3 0 51 184 41 847.3 0 41 185 33 847.3 0 33 186 35 847.3 0 35 187 34 847.3 0 34 188 55 847.3 0 55 189 34 847.3 0 34 190 26 847.3 0 26 191 39 847.3 0 39 192 37 847.3 0 37 193 40 847.3 0 40 194 45 847.3 0 45 195 37 847.3 0 37 196 40 847.3 0 40 197 31 847.3 0 31 198 32 847.3 0 32 199 36 847.3 0 36 200 36 847.3 0 36 201 16 847.3 0 16 202 33 847.3 0 33 203 58 847.3 0 58 204 59 847.3 0 59 205 61 847.3 0 61 206 45 847.3 0 45 207 43 847.3 0 43 208 57 847.3 0 57 209 63 847.3 0 63 210 47 847.3 0 47 211 50 847.3 0 50 212 39 847.3 0 39 213 34 847.3 0 34 214 41 847.3 0 41 215 50 847.3 0 50 216 24 847.3 0 24 217 50 847.3 0 50 218 39 847.3 0 39 219 68 847.3 0 68 220 60 847.3 0 60 221 64 847.3 0 64 222 75 847.3 0 75 223 61 847.3 0 61 224 63 847.3 0 63 225 42 847.3 0 42 226 42 847.3 0 42 227 42 847.3 0 42 228 65 847.3 0 65 229 108 847.3 0 108 230 85 847.3 0 85 231 51 847.3 0 51 232 40 847.3 0 40 233 24 847.3 0 24 234 37 847.3 0 37 235 29 847.3 0 29 236 34 847.3 0 34 237 43 847.3 0 43 238 47 847.3 0 47 239 58 847.3 0 58 240 63 847.3 0 63 241 45 847.3 0 45 242 40 847.3 0 40 243 33 847.3 0 33 244 38 847.3 0 38 245 43 847.3 0 43 246 46 847.3 0 46 247 62 847.3 0 62 248 51 847.3 0 51 249 38 847.3 0 38 250 37 847.3 0 37 251 38 847.3 0 38 252 42 847.3 0 42 253 67 847.3 0 67 254 75 847.3 0 75 255 111 847.3 0 111 256 137 847.3 0 137 257 103 847.3 0 103 258 76 847.3 0 76 259 68 847.3 0 68 260 54 847.3 0 54 261 52 847.3 0 52 262 138 847.3 0 138 263 164 847.3 0 164 264 186 847.3 0 186 265 39 847.3 0 39 266 54 847.3 0 54 267 52 847.3 0 52 268 61 847.3 0 61 269 51 847.3 0 51 270 34 847.3 0 34 271 44 847.3 0 44 272 45 847.3 0 45 273 61 847.3 0 61 274 51 847.3 0 51 275 63 847.3 0 63 276 40 847.3 0 40 277 35 847.3 0 35 278 110 847.3 0 110 279 125 847.3 0 125 280 86 847.3 0 86 281 45 847.3 0 45 282 74 847.3 0 74 283 97 847.3 0 97 284 144 847.3 0 144 285 149 847.3 0 149 286 41 847.3 0 41 287 57 847.3 0 57 288 59 847.3 0 59 289 55 847.3 0 55 290 72 847.3 0 72 291 138 847.3 0 138 292 141 847.3 0 141 293 108 847.3 0 108 294 107 847.3 0 107 295 55 847.3 0 55 296 54 847.3 0 54 297 45 847.3 0 45 298 48 847.3 0 48 299 90 847.3 0 90 300 183 847.3 0 183 301 159 847.3 0 159 Finished in 15.80 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 215,546 Reads with adapters: 84,158 (39.0%) Reads that were too short: 76 (0.0%) Reads written (passing filters): 84,082 (39.0%) Total basepairs processed: 59,645,653 bp Total written (filtered): 23,071,951 bp (38.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84158 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 50 0.0 3 0 0 40 10 16 880 0.0 3 0 789 59 32 17 81397 0.0 3 77029 3614 605 149 18 251 0.0 3 12 198 18 23 19 920 0.0 3 771 37 73 39 20 212 0.0 3 0 1 0 211 21 2 0.0 3 0 0 0 2 22 2 0.0 3 0 0 0 2 23 8 0.0 3 0 0 0 8 26 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 28 2 0.0 3 1 0 0 1 29 6 0.0 3 1 0 1 4 30 14 0.0 3 0 0 0 14 31 2 0.0 3 0 0 1 1 33 4 0.0 3 0 0 0 4 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 10 0.0 3 0 0 0 10 50 12 0.0 3 0 0 1 11 51 11 0.0 3 0 0 1 10 52 9 0.0 3 0 0 0 9 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 6 0.0 3 0 0 0 6 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 1 72 44 0.0 3 0 0 0 44 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 97 4 0.0 3 0 0 0 4 98 159 0.0 3 0 0 0 159 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 158 7 0.0 3 0 0 0 7 159 3 0.0 3 0 0 0 3 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 5 0.0 3 0 0 0 5 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 201 1 0.0 3 1 203 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 0 1 259 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 7 0.0 3 0 0 0 7 281 7 0.0 3 0 0 0 7 282 6 0.0 3 0 0 0 6 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.96 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 84,082 Reads with adapters: 5,693 (6.8%) Reads written (passing filters): 84,082 (100.0%) Total basepairs processed: 23,071,951 bp Total written (filtered): 23,054,829 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5693 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.9% C: 11.3% G: 9.7% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5657 1313.8 0 5657 4 30 328.4 0 30 5 5 82.1 0 5 6 1 20.5 0 1