This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.84 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 345,538 (10.0%) Reads that were too short: 14,640 (0.4%) Reads written (passing filters): 330,898 (9.5%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 92,140,667 bp (8.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 345538 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2470 847.3 0 2470 7 4133 847.3 0 4133 8 150802 847.3 0 150802 9 127673 847.3 0 127673 10 205 847.3 0 205 11 77 847.3 0 77 12 45 847.3 0 45 13 43 847.3 0 43 14 45 847.3 0 45 15 66 847.3 0 66 16 52 847.3 0 52 17 42 847.3 0 42 18 41 847.3 0 41 19 37 847.3 0 37 20 41 847.3 0 41 21 55 847.3 0 55 22 40 847.3 0 40 23 53 847.3 0 53 24 44 847.3 0 44 25 40 847.3 0 40 26 49 847.3 0 49 27 62 847.3 0 62 28 52 847.3 0 52 29 47 847.3 0 47 30 38 847.3 0 38 31 53 847.3 0 53 32 73 847.3 0 73 33 32 847.3 0 32 34 36 847.3 0 36 35 56 847.3 0 56 36 50 847.3 0 50 37 57 847.3 0 57 38 59 847.3 0 59 39 62 847.3 0 62 40 34 847.3 0 34 41 55 847.3 0 55 42 91 847.3 0 91 43 59 847.3 0 59 44 51 847.3 0 51 45 138 847.3 0 138 46 105 847.3 0 105 47 76 847.3 0 76 48 41 847.3 0 41 49 46 847.3 0 46 50 53 847.3 0 53 51 51 847.3 0 51 52 25 847.3 0 25 53 45 847.3 0 45 54 50 847.3 0 50 55 55 847.3 0 55 56 44 847.3 0 44 57 82 847.3 0 82 58 59 847.3 0 59 59 92 847.3 0 92 60 54 847.3 0 54 61 56 847.3 0 56 62 85 847.3 0 85 63 55 847.3 0 55 64 77 847.3 0 77 65 138 847.3 0 138 66 197 847.3 0 197 67 81 847.3 0 81 68 118 847.3 0 118 69 137 847.3 0 137 70 99 847.3 0 99 71 154 847.3 0 154 72 924 847.3 0 924 73 536 847.3 0 536 74 310 847.3 0 310 75 409 847.3 0 409 76 270 847.3 0 270 77 299 847.3 0 299 78 526 847.3 0 526 79 7282 847.3 0 7282 80 3611 847.3 0 3611 81 3071 847.3 0 3071 82 291 847.3 0 291 83 287 847.3 0 287 84 247 847.3 0 247 85 98 847.3 0 98 86 74 847.3 0 74 87 61 847.3 0 61 88 97 847.3 0 97 89 64 847.3 0 64 90 59 847.3 0 59 91 58 847.3 0 58 92 84 847.3 0 84 93 76 847.3 0 76 94 60 847.3 0 60 95 114 847.3 0 114 96 98 847.3 0 98 97 88 847.3 0 88 98 405 847.3 0 405 99 310 847.3 0 310 100 137 847.3 0 137 101 115 847.3 0 115 102 149 847.3 0 149 103 141 847.3 0 141 104 123 847.3 0 123 105 122 847.3 0 122 106 127 847.3 0 127 107 158 847.3 0 158 108 166 847.3 0 166 109 155 847.3 0 155 110 197 847.3 0 197 111 198 847.3 0 198 112 179 847.3 0 179 113 197 847.3 0 197 114 183 847.3 0 183 115 213 847.3 0 213 116 230 847.3 0 230 117 239 847.3 0 239 118 207 847.3 0 207 119 225 847.3 0 225 120 195 847.3 0 195 121 226 847.3 0 226 122 170 847.3 0 170 123 205 847.3 0 205 124 329 847.3 0 329 125 649 847.3 0 649 126 388 847.3 0 388 127 325 847.3 0 325 128 297 847.3 0 297 129 247 847.3 0 247 130 241 847.3 0 241 131 181 847.3 0 181 132 151 847.3 0 151 133 125 847.3 0 125 134 162 847.3 0 162 135 170 847.3 0 170 136 197 847.3 0 197 137 190 847.3 0 190 138 592 847.3 0 592 139 407 847.3 0 407 140 317 847.3 0 317 141 606 847.3 0 606 142 475 847.3 0 475 143 175 847.3 0 175 144 193 847.3 0 193 145 146 847.3 0 146 146 146 847.3 0 146 147 147 847.3 0 147 148 116 847.3 0 116 149 120 847.3 0 120 150 229 847.3 0 229 151 219 847.3 0 219 152 130 847.3 0 130 153 106 847.3 0 106 154 116 847.3 0 116 155 127 847.3 0 127 156 137 847.3 0 137 157 123 847.3 0 123 158 282 847.3 0 282 159 249 847.3 0 249 160 456 847.3 0 456 161 112 847.3 0 112 162 133 847.3 0 133 163 133 847.3 0 133 164 231 847.3 0 231 165 136 847.3 0 136 166 174 847.3 0 174 167 114 847.3 0 114 168 124 847.3 0 124 169 132 847.3 0 132 170 142 847.3 0 142 171 116 847.3 0 116 172 128 847.3 0 128 173 95 847.3 0 95 174 100 847.3 0 100 175 95 847.3 0 95 176 92 847.3 0 92 177 127 847.3 0 127 178 134 847.3 0 134 179 153 847.3 0 153 180 153 847.3 0 153 181 153 847.3 0 153 182 837 847.3 0 837 183 1214 847.3 0 1214 184 1622 847.3 0 1622 185 150 847.3 0 150 186 94 847.3 0 94 187 99 847.3 0 99 188 125 847.3 0 125 189 122 847.3 0 122 190 106 847.3 0 106 191 93 847.3 0 93 192 90 847.3 0 90 193 103 847.3 0 103 194 122 847.3 0 122 195 87 847.3 0 87 196 99 847.3 0 99 197 116 847.3 0 116 198 108 847.3 0 108 199 109 847.3 0 109 200 117 847.3 0 117 201 143 847.3 0 143 202 107 847.3 0 107 203 111 847.3 0 111 204 138 847.3 0 138 205 129 847.3 0 129 206 166 847.3 0 166 207 167 847.3 0 167 208 215 847.3 0 215 209 181 847.3 0 181 210 167 847.3 0 167 211 177 847.3 0 177 212 188 847.3 0 188 213 171 847.3 0 171 214 129 847.3 0 129 215 112 847.3 0 112 216 119 847.3 0 119 217 97 847.3 0 97 218 111 847.3 0 111 219 110 847.3 0 110 220 104 847.3 0 104 221 107 847.3 0 107 222 150 847.3 0 150 223 115 847.3 0 115 224 107 847.3 0 107 225 137 847.3 0 137 226 186 847.3 0 186 227 207 847.3 0 207 228 174 847.3 0 174 229 103 847.3 0 103 230 100 847.3 0 100 231 174 847.3 0 174 232 128 847.3 0 128 233 124 847.3 0 124 234 105 847.3 0 105 235 131 847.3 0 131 236 97 847.3 0 97 237 204 847.3 0 204 238 157 847.3 0 157 239 118 847.3 0 118 240 105 847.3 0 105 241 124 847.3 0 124 242 107 847.3 0 107 243 111 847.3 0 111 244 87 847.3 0 87 245 108 847.3 0 108 246 108 847.3 0 108 247 147 847.3 0 147 248 113 847.3 0 113 249 123 847.3 0 123 250 135 847.3 0 135 251 118 847.3 0 118 252 116 847.3 0 116 253 267 847.3 0 267 254 361 847.3 0 361 255 763 847.3 0 763 256 596 847.3 0 596 257 126 847.3 0 126 258 111 847.3 0 111 259 111 847.3 0 111 260 158 847.3 0 158 261 138 847.3 0 138 262 145 847.3 0 145 263 155 847.3 0 155 264 431 847.3 0 431 265 178 847.3 0 178 266 152 847.3 0 152 267 50 847.3 0 50 268 107 847.3 0 107 269 117 847.3 0 117 270 107 847.3 0 107 271 81 847.3 0 81 272 117 847.3 0 117 273 103 847.3 0 103 274 109 847.3 0 109 275 137 847.3 0 137 276 170 847.3 0 170 277 127 847.3 0 127 278 102 847.3 0 102 279 104 847.3 0 104 280 101 847.3 0 101 281 110 847.3 0 110 282 104 847.3 0 104 283 90 847.3 0 90 284 136 847.3 0 136 285 141 847.3 0 141 286 117 847.3 0 117 287 106 847.3 0 106 288 105 847.3 0 105 289 151 847.3 0 151 290 136 847.3 0 136 291 114 847.3 0 114 292 124 847.3 0 124 293 87 847.3 0 87 294 94 847.3 0 94 295 106 847.3 0 106 296 117 847.3 0 117 297 128 847.3 0 128 298 113 847.3 0 113 299 128 847.3 0 128 300 144 847.3 0 144 301 240 847.3 0 240 Finished in 24.36 s (74 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 330,898 Reads with adapters: 150,926 (45.6%) Reads that were too short: 208 (0.1%) Reads written (passing filters): 150,718 (45.5%) Total basepairs processed: 92,140,667 bp Total written (filtered): 41,571,727 bp (45.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 150926 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 22 0.0 2 0 0 0 22 15 75 0.0 3 0 0 56 19 16 1559 0.0 3 0 1378 137 44 17 147803 0.0 3 140877 5735 995 196 18 382 0.0 3 25 268 39 50 19 263 0.0 3 8 0 163 92 20 408 0.0 3 1 0 0 407 21 8 0.0 3 1 0 0 7 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 7 0.0 3 0 0 0 7 26 1 0.0 3 1 27 1 0.0 3 1 28 10 0.0 3 1 0 0 9 31 3 0.0 3 0 0 0 3 32 3 0.0 3 1 0 0 2 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 12 0.0 3 0 0 0 12 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 1 44 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 7 0.0 3 0 0 0 7 52 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 1 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 65 10 0.0 3 0 0 0 10 66 1 0.0 3 0 0 0 1 67 5 0.0 3 0 0 0 5 68 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 111 1 0.0 3 0 0 0 1 112 50 0.0 3 0 0 1 49 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 8 0.0 3 0 0 1 7 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 134 8 0.0 3 0 0 0 8 135 3 0.0 3 1 0 0 2 136 4 0.0 3 0 0 0 4 137 2 0.0 3 0 0 0 2 138 5 0.0 3 0 0 0 5 140 1 0.0 3 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 7 0.0 3 0 0 0 7 148 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 6 0.0 3 0 0 0 6 161 3 0.0 3 0 0 0 3 163 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 165 3 0.0 3 0 0 0 3 166 1 0.0 3 0 0 0 1 172 22 0.0 3 0 0 0 22 173 5 0.0 3 0 0 0 5 175 4 0.0 3 0 0 0 4 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 188 1 0.0 3 0 0 1 191 2 0.0 3 0 0 0 2 192 5 0.0 3 0 0 0 5 194 5 0.0 3 0 0 0 5 195 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 1 213 5 0.0 3 0 0 0 5 214 21 0.0 3 0 0 4 17 216 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 247 1 0.0 3 0 1 254 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 5 0.0 3 0 0 0 5 281 13 0.0 3 0 0 0 13 282 7 0.0 3 0 0 0 7 283 5 0.0 3 0 0 0 5 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.02 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 150,718 Reads with adapters: 6,523 (4.3%) Reads written (passing filters): 150,718 (100.0%) Total basepairs processed: 41,571,727 bp Total written (filtered): 41,551,951 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6523 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.7% C: 11.3% G: 7.5% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6330 2355.0 0 6330 4 183 588.7 0 183 5 6 147.2 0 6 6 4 36.8 0 4