This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.97 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 222,278 (6.4%) Reads that were too short: 12,351 (0.4%) Reads written (passing filters): 209,927 (6.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 59,846,558 bp (5.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 222278 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2036 847.3 0 2036 7 93561 847.3 0 93561 8 99624 847.3 0 99624 9 81 847.3 0 81 10 39 847.3 0 39 11 28 847.3 0 28 12 24 847.3 0 24 13 31 847.3 0 31 14 22 847.3 0 22 15 22 847.3 0 22 16 29 847.3 0 29 17 37 847.3 0 37 18 39 847.3 0 39 19 25 847.3 0 25 20 19 847.3 0 19 21 16 847.3 0 16 22 20 847.3 0 20 23 22 847.3 0 22 24 20 847.3 0 20 25 18 847.3 0 18 26 17 847.3 0 17 27 16 847.3 0 16 28 46 847.3 0 46 29 17 847.3 0 17 30 21 847.3 0 21 31 20 847.3 0 20 32 19 847.3 0 19 33 19 847.3 0 19 34 23 847.3 0 23 35 25 847.3 0 25 36 24 847.3 0 24 37 27 847.3 0 27 38 35 847.3 0 35 39 29 847.3 0 29 40 16 847.3 0 16 41 33 847.3 0 33 42 21 847.3 0 21 43 24 847.3 0 24 44 22 847.3 0 22 45 15 847.3 0 15 46 25 847.3 0 25 47 25 847.3 0 25 48 25 847.3 0 25 49 24 847.3 0 24 50 25 847.3 0 25 51 27 847.3 0 27 52 19 847.3 0 19 53 17 847.3 0 17 54 19 847.3 0 19 55 16 847.3 0 16 56 21 847.3 0 21 57 13 847.3 0 13 58 23 847.3 0 23 59 24 847.3 0 24 60 20 847.3 0 20 61 24 847.3 0 24 62 25 847.3 0 25 63 26 847.3 0 26 64 32 847.3 0 32 65 30 847.3 0 30 66 16 847.3 0 16 67 15 847.3 0 15 68 22 847.3 0 22 69 28 847.3 0 28 70 13 847.3 0 13 71 26 847.3 0 26 72 18 847.3 0 18 73 13 847.3 0 13 74 19 847.3 0 19 75 38 847.3 0 38 76 25 847.3 0 25 77 30 847.3 0 30 78 48 847.3 0 48 79 51 847.3 0 51 80 68 847.3 0 68 81 66 847.3 0 66 82 53 847.3 0 53 83 78 847.3 0 78 84 70 847.3 0 70 85 65 847.3 0 65 86 68 847.3 0 68 87 44 847.3 0 44 88 60 847.3 0 60 89 48 847.3 0 48 90 62 847.3 0 62 91 78 847.3 0 78 92 104 847.3 0 104 93 85 847.3 0 85 94 84 847.3 0 84 95 89 847.3 0 89 96 72 847.3 0 72 97 81 847.3 0 81 98 144 847.3 0 144 99 142 847.3 0 142 100 164 847.3 0 164 101 93 847.3 0 93 102 79 847.3 0 79 103 71 847.3 0 71 104 73 847.3 0 73 105 94 847.3 0 94 106 112 847.3 0 112 107 146 847.3 0 146 108 351 847.3 0 351 109 280 847.3 0 280 110 268 847.3 0 268 111 109 847.3 0 109 112 157 847.3 0 157 113 117 847.3 0 117 114 235 847.3 0 235 115 179 847.3 0 179 116 257 847.3 0 257 117 95 847.3 0 95 118 78 847.3 0 78 119 47 847.3 0 47 120 68 847.3 0 68 121 76 847.3 0 76 122 120 847.3 0 120 123 142 847.3 0 142 124 115 847.3 0 115 125 87 847.3 0 87 126 72 847.3 0 72 127 77 847.3 0 77 128 69 847.3 0 69 129 123 847.3 0 123 130 130 847.3 0 130 131 183 847.3 0 183 132 115 847.3 0 115 133 118 847.3 0 118 134 121 847.3 0 121 135 124 847.3 0 124 136 134 847.3 0 134 137 172 847.3 0 172 138 114 847.3 0 114 139 121 847.3 0 121 140 123 847.3 0 123 141 131 847.3 0 131 142 82 847.3 0 82 143 93 847.3 0 93 144 96 847.3 0 96 145 103 847.3 0 103 146 86 847.3 0 86 147 73 847.3 0 73 148 82 847.3 0 82 149 93 847.3 0 93 150 100 847.3 0 100 151 91 847.3 0 91 152 98 847.3 0 98 153 107 847.3 0 107 154 97 847.3 0 97 155 175 847.3 0 175 156 129 847.3 0 129 157 138 847.3 0 138 158 89 847.3 0 89 159 83 847.3 0 83 160 100 847.3 0 100 161 87 847.3 0 87 162 84 847.3 0 84 163 119 847.3 0 119 164 128 847.3 0 128 165 140 847.3 0 140 166 91 847.3 0 91 167 121 847.3 0 121 168 91 847.3 0 91 169 102 847.3 0 102 170 90 847.3 0 90 171 86 847.3 0 86 172 86 847.3 0 86 173 66 847.3 0 66 174 54 847.3 0 54 175 78 847.3 0 78 176 94 847.3 0 94 177 82 847.3 0 82 178 124 847.3 0 124 179 122 847.3 0 122 180 89 847.3 0 89 181 94 847.3 0 94 182 112 847.3 0 112 183 106 847.3 0 106 184 99 847.3 0 99 185 81 847.3 0 81 186 79 847.3 0 79 187 76 847.3 0 76 188 101 847.3 0 101 189 94 847.3 0 94 190 100 847.3 0 100 191 117 847.3 0 117 192 101 847.3 0 101 193 75 847.3 0 75 194 90 847.3 0 90 195 88 847.3 0 88 196 88 847.3 0 88 197 93 847.3 0 93 198 63 847.3 0 63 199 73 847.3 0 73 200 112 847.3 0 112 201 131 847.3 0 131 202 153 847.3 0 153 203 152 847.3 0 152 204 106 847.3 0 106 205 123 847.3 0 123 206 102 847.3 0 102 207 112 847.3 0 112 208 124 847.3 0 124 209 187 847.3 0 187 210 209 847.3 0 209 211 157 847.3 0 157 212 81 847.3 0 81 213 76 847.3 0 76 214 74 847.3 0 74 215 71 847.3 0 71 216 114 847.3 0 114 217 88 847.3 0 88 218 85 847.3 0 85 219 104 847.3 0 104 220 82 847.3 0 82 221 98 847.3 0 98 222 123 847.3 0 123 223 128 847.3 0 128 224 149 847.3 0 149 225 105 847.3 0 105 226 93 847.3 0 93 227 89 847.3 0 89 228 79 847.3 0 79 229 80 847.3 0 80 230 86 847.3 0 86 231 80 847.3 0 80 232 89 847.3 0 89 233 69 847.3 0 69 234 60 847.3 0 60 235 77 847.3 0 77 236 97 847.3 0 97 237 121 847.3 0 121 238 90 847.3 0 90 239 130 847.3 0 130 240 103 847.3 0 103 241 120 847.3 0 120 242 157 847.3 0 157 243 158 847.3 0 158 244 173 847.3 0 173 245 163 847.3 0 163 246 224 847.3 0 224 247 110 847.3 0 110 248 90 847.3 0 90 249 77 847.3 0 77 250 87 847.3 0 87 251 85 847.3 0 85 252 132 847.3 0 132 253 268 847.3 0 268 254 301 847.3 0 301 255 559 847.3 0 559 256 140 847.3 0 140 257 80 847.3 0 80 258 108 847.3 0 108 259 150 847.3 0 150 260 140 847.3 0 140 261 84 847.3 0 84 262 91 847.3 0 91 263 191 847.3 0 191 264 198 847.3 0 198 265 223 847.3 0 223 266 75 847.3 0 75 267 63 847.3 0 63 268 61 847.3 0 61 269 127 847.3 0 127 270 133 847.3 0 133 271 207 847.3 0 207 272 235 847.3 0 235 273 155 847.3 0 155 274 120 847.3 0 120 275 89 847.3 0 89 276 94 847.3 0 94 277 93 847.3 0 93 278 65 847.3 0 65 279 59 847.3 0 59 280 73 847.3 0 73 281 119 847.3 0 119 282 110 847.3 0 110 283 116 847.3 0 116 284 155 847.3 0 155 285 159 847.3 0 159 286 101 847.3 0 101 287 78 847.3 0 78 288 73 847.3 0 73 289 91 847.3 0 91 290 213 847.3 0 213 291 194 847.3 0 194 292 125 847.3 0 125 293 109 847.3 0 109 294 71 847.3 0 71 295 67 847.3 0 67 296 102 847.3 0 102 297 98 847.3 0 98 298 130 847.3 0 130 299 129 847.3 0 129 300 112 847.3 0 112 301 95 847.3 0 95 Finished in 16.71 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 209,927 Reads with adapters: 102,056 (48.6%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 102,025 (48.6%) Total basepairs processed: 59,846,558 bp Total written (filtered): 27,831,135 bp (46.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 102056 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 36 0.0 3 0 0 33 3 19 783 0.0 3 0 761 13 5 4 20 100628 0.0 4 99030 1544 49 4 1 21 243 0.0 4 41 198 3 1 22 34 0.0 4 11 0 21 2 23 68 0.0 4 0 0 0 61 7 24 5 0.0 4 1 0 0 0 4 25 2 0.0 4 2 26 5 0.0 4 1 0 0 0 4 39 1 0.0 4 1 41 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 1 77 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 1 0.0 4 1 123 1 0.0 4 1 126 2 0.0 4 0 0 0 0 2 127 198 0.0 4 0 0 0 0 198 128 5 0.0 4 0 0 0 0 5 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 184 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 218 2 0.0 4 1 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 277 1 0.0 4 1 279 1 0.0 4 0 0 1 281 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.03 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 102,025 Reads with adapters: 6,548 (6.4%) Reads written (passing filters): 102,025 (100.0%) Total basepairs processed: 27,831,135 bp Total written (filtered): 27,726,181 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6548 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 3.2% G: 91.2% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 68 1594.1 0 68 4 23 398.5 0 23 5 5 99.6 0 5 8 1 1.6 0 1 9 128 0.4 0 2 126 10 2105 0.1 1 61 2044 11 4021 0.0 1 26 3995 12 13 0.0 1 2 11 13 1 0.0 1 0 1 14 7 0.0 1 5 2 19 2 0.0 1 2 123 1 0.0 1 0 1 151 9 0.0 1 0 9 190 2 0.0 1 0 2 209 16 0.0 1 7 9 210 2 0.0 1 1 1 223 2 0.0 1 1 1 224 136 0.0 1 124 12 225 5 0.0 1 5 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.