This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 166.19 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 102,070 (2.9%) Reads that were too short: 51,825 (1.5%) Reads written (passing filters): 50,245 (1.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 12,363,901 bp (1.2%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 102070 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1155 847.3 0 1155 7 10742 847.3 0 10742 8 432 847.3 0 432 9 13627 847.3 0 13627 10 97 847.3 0 97 11 84 847.3 0 84 12 76 847.3 0 76 13 81 847.3 0 81 14 82 847.3 0 82 15 62 847.3 0 62 16 49 847.3 0 49 17 145 847.3 0 145 18 167 847.3 0 167 19 89 847.3 0 89 20 54 847.3 0 54 21 91 847.3 0 91 22 82 847.3 0 82 23 65 847.3 0 65 24 67 847.3 0 67 25 52 847.3 0 52 26 65 847.3 0 65 27 50 847.3 0 50 28 65 847.3 0 65 29 56 847.3 0 56 30 53 847.3 0 53 31 65 847.3 0 65 32 59 847.3 0 59 33 73 847.3 0 73 34 63 847.3 0 63 35 61 847.3 0 61 36 49 847.3 0 49 37 81 847.3 0 81 38 88 847.3 0 88 39 96 847.3 0 96 40 68 847.3 0 68 41 71 847.3 0 71 42 89 847.3 0 89 43 92 847.3 0 92 44 90 847.3 0 90 45 98 847.3 0 98 46 85 847.3 0 85 47 713 847.3 0 713 48 652 847.3 0 652 49 519 847.3 0 519 50 223 847.3 0 223 51 57 847.3 0 57 52 76 847.3 0 76 53 53 847.3 0 53 54 81 847.3 0 81 55 73 847.3 0 73 56 83 847.3 0 83 57 76 847.3 0 76 58 98 847.3 0 98 59 296 847.3 0 296 60 260 847.3 0 260 61 213 847.3 0 213 62 131 847.3 0 131 63 70 847.3 0 70 64 68 847.3 0 68 65 63 847.3 0 63 66 70 847.3 0 70 67 54 847.3 0 54 68 59 847.3 0 59 69 85 847.3 0 85 70 90 847.3 0 90 71 89 847.3 0 89 72 93 847.3 0 93 73 68 847.3 0 68 74 72 847.3 0 72 75 73 847.3 0 73 76 95 847.3 0 95 77 399 847.3 0 399 78 218 847.3 0 218 79 131 847.3 0 131 80 136 847.3 0 136 81 84 847.3 0 84 82 92 847.3 0 92 83 156 847.3 0 156 84 596 847.3 0 596 85 431 847.3 0 431 86 409 847.3 0 409 87 95 847.3 0 95 88 66 847.3 0 66 89 160 847.3 0 160 90 100 847.3 0 100 91 81 847.3 0 81 92 60 847.3 0 60 93 59 847.3 0 59 94 64 847.3 0 64 95 53 847.3 0 53 96 63 847.3 0 63 97 44 847.3 0 44 98 48 847.3 0 48 99 63 847.3 0 63 100 71 847.3 0 71 101 56 847.3 0 56 102 59 847.3 0 59 103 47 847.3 0 47 104 43 847.3 0 43 105 52 847.3 0 52 106 81 847.3 0 81 107 73 847.3 0 73 108 54 847.3 0 54 109 65 847.3 0 65 110 68 847.3 0 68 111 86 847.3 0 86 112 372 847.3 0 372 113 594 847.3 0 594 114 178 847.3 0 178 115 54 847.3 0 54 116 67 847.3 0 67 117 127 847.3 0 127 118 74 847.3 0 74 119 74 847.3 0 74 120 80 847.3 0 80 121 61 847.3 0 61 122 56 847.3 0 56 123 64 847.3 0 64 124 77 847.3 0 77 125 74 847.3 0 74 126 92 847.3 0 92 127 153 847.3 0 153 128 106 847.3 0 106 129 163 847.3 0 163 130 138 847.3 0 138 131 131 847.3 0 131 132 412 847.3 0 412 133 620 847.3 0 620 134 835 847.3 0 835 135 339 847.3 0 339 136 218 847.3 0 218 137 94 847.3 0 94 138 97 847.3 0 97 139 119 847.3 0 119 140 126 847.3 0 126 141 703 847.3 0 703 142 401 847.3 0 401 143 376 847.3 0 376 144 107 847.3 0 107 145 69 847.3 0 69 146 86 847.3 0 86 147 77 847.3 0 77 148 121 847.3 0 121 149 108 847.3 0 108 150 65 847.3 0 65 151 107 847.3 0 107 152 474 847.3 0 474 153 441 847.3 0 441 154 296 847.3 0 296 155 115 847.3 0 115 156 56 847.3 0 56 157 68 847.3 0 68 158 51 847.3 0 51 159 49 847.3 0 49 160 48 847.3 0 48 161 54 847.3 0 54 162 58 847.3 0 58 163 66 847.3 0 66 164 53 847.3 0 53 165 63 847.3 0 63 166 66 847.3 0 66 167 49 847.3 0 49 168 52 847.3 0 52 169 61 847.3 0 61 170 68 847.3 0 68 171 66 847.3 0 66 172 76 847.3 0 76 173 63 847.3 0 63 174 67 847.3 0 67 175 65 847.3 0 65 176 65 847.3 0 65 177 54 847.3 0 54 178 54 847.3 0 54 179 72 847.3 0 72 180 62 847.3 0 62 181 57 847.3 0 57 182 58 847.3 0 58 183 55 847.3 0 55 184 73 847.3 0 73 185 67 847.3 0 67 186 79 847.3 0 79 187 160 847.3 0 160 188 81 847.3 0 81 189 104 847.3 0 104 190 93 847.3 0 93 191 75 847.3 0 75 192 99 847.3 0 99 193 90 847.3 0 90 194 67 847.3 0 67 195 75 847.3 0 75 196 91 847.3 0 91 197 69 847.3 0 69 198 92 847.3 0 92 199 116 847.3 0 116 200 80 847.3 0 80 201 85 847.3 0 85 202 104 847.3 0 104 203 178 847.3 0 178 204 81 847.3 0 81 205 118 847.3 0 118 206 106 847.3 0 106 207 95 847.3 0 95 208 69 847.3 0 69 209 66 847.3 0 66 210 67 847.3 0 67 211 105 847.3 0 105 212 202 847.3 0 202 213 194 847.3 0 194 214 179 847.3 0 179 215 135 847.3 0 135 216 172 847.3 0 172 217 174 847.3 0 174 218 277 847.3 0 277 219 388 847.3 0 388 220 223 847.3 0 223 221 112 847.3 0 112 222 73 847.3 0 73 223 50 847.3 0 50 224 83 847.3 0 83 225 103 847.3 0 103 226 106 847.3 0 106 227 92 847.3 0 92 228 96 847.3 0 96 229 182 847.3 0 182 230 373 847.3 0 373 231 182 847.3 0 182 232 125 847.3 0 125 233 153 847.3 0 153 234 181 847.3 0 181 235 927 847.3 0 927 236 912 847.3 0 912 237 1031 847.3 0 1031 238 559 847.3 0 559 239 401 847.3 0 401 240 142 847.3 0 142 241 98 847.3 0 98 242 105 847.3 0 105 243 124 847.3 0 124 244 192 847.3 0 192 245 366 847.3 0 366 246 576 847.3 0 576 247 554 847.3 0 554 248 378 847.3 0 378 249 211 847.3 0 211 250 216 847.3 0 216 251 170 847.3 0 170 252 367 847.3 0 367 253 242 847.3 0 242 254 218 847.3 0 218 255 910 847.3 0 910 256 1257 847.3 0 1257 257 978 847.3 0 978 258 316 847.3 0 316 259 91 847.3 0 91 260 142 847.3 0 142 261 179 847.3 0 179 262 111 847.3 0 111 263 86 847.3 0 86 264 130 847.3 0 130 265 85 847.3 0 85 266 91 847.3 0 91 267 79 847.3 0 79 268 108 847.3 0 108 269 87 847.3 0 87 270 78 847.3 0 78 271 92 847.3 0 92 272 146 847.3 0 146 273 769 847.3 0 769 274 478 847.3 0 478 275 205 847.3 0 205 276 307 847.3 0 307 277 138 847.3 0 138 278 216 847.3 0 216 279 202 847.3 0 202 280 234 847.3 0 234 281 191 847.3 0 191 282 297 847.3 0 297 283 452 847.3 0 452 284 747 847.3 0 747 285 889 847.3 0 889 286 706 847.3 0 706 287 620 847.3 0 620 288 485 847.3 0 485 289 407 847.3 0 407 290 262 847.3 0 262 291 283 847.3 0 283 292 273 847.3 0 273 293 324 847.3 0 324 294 330 847.3 0 330 295 408 847.3 0 408 296 567 847.3 0 567 297 859 847.3 0 859 298 10157 847.3 0 10157 299 8116 847.3 0 8116 300 4590 847.3 0 4590 301 714 847.3 0 714 Finished in 3.51 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 50,245 Reads with adapters: 14,696 (29.2%) Reads that were too short: 124 (0.2%) Reads written (passing filters): 14,572 (29.0%) Total basepairs processed: 12,363,901 bp Total written (filtered): 3,951,317 bp (32.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14696 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 7 2 16 109 0.0 3 0 93 13 3 17 13967 0.0 3 13158 668 111 30 18 32 0.0 3 3 22 4 3 19 24 0.0 3 1 0 17 6 20 46 0.0 3 1 0 0 45 21 3 0.0 3 1 0 0 2 22 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 36 2 0.0 3 1 0 0 1 37 1 0.0 3 0 0 0 1 38 5 0.0 3 0 0 0 5 39 65 0.0 3 0 0 0 65 41 1 0.0 3 0 0 0 1 42 18 0.0 3 0 0 0 18 44 1 0.0 3 0 0 0 1 45 9 0.0 3 0 0 0 9 46 2 0.0 3 0 0 0 2 47 5 0.0 3 0 0 1 4 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 34 0.0 3 0 0 0 34 51 1 0.0 3 0 0 1 52 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 6 0.0 3 0 0 0 6 66 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 1 76 6 0.0 3 0 0 0 6 79 18 0.0 3 0 0 0 18 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 1 1 87 20 0.0 3 0 0 1 19 88 4 0.0 3 0 0 0 4 89 4 0.0 3 0 0 0 4 90 2 0.0 3 0 0 0 2 91 5 0.0 3 0 0 0 5 92 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 0 4 95 5 0.0 3 0 0 0 5 96 5 0.0 3 0 0 0 5 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 49 0.0 3 0 0 0 49 102 5 0.0 3 0 0 0 5 103 2 0.0 3 0 0 1 1 104 3 0.0 3 0 0 0 3 105 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 107 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 15 0.0 3 0 0 0 15 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 11 0.0 3 0 0 1 10 125 1 0.0 3 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 15 0.0 3 0 0 0 15 135 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 143 34 0.0 3 0 0 0 34 144 2 0.0 3 0 0 0 2 145 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 6 0.0 3 0 0 0 6 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 3 0.0 3 0 0 0 3 170 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 196 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 201 2 0.0 3 0 0 0 2 202 2 0.0 3 0 0 1 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 3 0.0 3 0 0 0 3 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 1 248 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 14,572 Reads with adapters: 810 (5.6%) Reads written (passing filters): 14,572 (100.0%) Total basepairs processed: 3,951,317 bp Total written (filtered): 3,948,881 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 810 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.6% C: 11.7% G: 12.2% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 804 227.7 0 804 4 6 56.9 0 6