This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.07 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 348,506 (10.0%) Reads that were too short: 33,463 (1.0%) Reads written (passing filters): 315,043 (9.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 90,597,927 bp (8.7%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 348506 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4666 847.3 0 4666 7 160962 847.3 0 160962 8 3070 847.3 0 3070 9 131930 847.3 0 131930 10 381 847.3 0 381 11 31 847.3 0 31 12 43 847.3 0 43 13 60 847.3 0 60 14 14 847.3 0 14 15 17 847.3 0 17 16 38 847.3 0 38 17 13 847.3 0 13 18 25 847.3 0 25 19 15 847.3 0 15 20 7 847.3 0 7 21 27 847.3 0 27 22 21 847.3 0 21 23 30 847.3 0 30 24 15 847.3 0 15 25 22 847.3 0 22 26 19 847.3 0 19 27 14 847.3 0 14 28 12 847.3 0 12 29 33 847.3 0 33 30 29 847.3 0 29 31 21 847.3 0 21 32 30 847.3 0 30 33 14 847.3 0 14 34 16 847.3 0 16 35 13 847.3 0 13 36 19 847.3 0 19 37 26 847.3 0 26 38 19 847.3 0 19 39 16 847.3 0 16 40 21 847.3 0 21 41 23 847.3 0 23 42 19 847.3 0 19 43 20 847.3 0 20 44 19 847.3 0 19 45 23 847.3 0 23 46 14 847.3 0 14 47 25 847.3 0 25 48 26 847.3 0 26 49 23 847.3 0 23 50 18 847.3 0 18 51 15 847.3 0 15 52 24 847.3 0 24 53 27 847.3 0 27 54 33 847.3 0 33 55 83 847.3 0 83 56 81 847.3 0 81 57 34 847.3 0 34 58 15 847.3 0 15 59 46 847.3 0 46 60 30 847.3 0 30 61 46 847.3 0 46 62 28 847.3 0 28 63 25 847.3 0 25 64 30 847.3 0 30 65 33 847.3 0 33 66 28 847.3 0 28 67 37 847.3 0 37 68 17 847.3 0 17 69 45 847.3 0 45 70 47 847.3 0 47 71 54 847.3 0 54 72 46 847.3 0 46 73 64 847.3 0 64 74 138 847.3 0 138 75 139 847.3 0 139 76 207 847.3 0 207 77 57 847.3 0 57 78 28 847.3 0 28 79 69 847.3 0 69 80 93 847.3 0 93 81 165 847.3 0 165 82 304 847.3 0 304 83 368 847.3 0 368 84 398 847.3 0 398 85 70 847.3 0 70 86 85 847.3 0 85 87 59 847.3 0 59 88 42 847.3 0 42 89 90 847.3 0 90 90 49 847.3 0 49 91 24 847.3 0 24 92 30 847.3 0 30 93 33 847.3 0 33 94 24 847.3 0 24 95 24 847.3 0 24 96 42 847.3 0 42 97 25 847.3 0 25 98 27 847.3 0 27 99 18 847.3 0 18 100 28 847.3 0 28 101 21 847.3 0 21 102 40 847.3 0 40 103 39 847.3 0 39 104 30 847.3 0 30 105 37 847.3 0 37 106 25 847.3 0 25 107 29 847.3 0 29 108 21 847.3 0 21 109 27 847.3 0 27 110 24 847.3 0 24 111 37 847.3 0 37 112 40 847.3 0 40 113 49 847.3 0 49 114 36 847.3 0 36 115 45 847.3 0 45 116 22 847.3 0 22 117 30 847.3 0 30 118 37 847.3 0 37 119 47 847.3 0 47 120 51 847.3 0 51 121 141 847.3 0 141 122 155 847.3 0 155 123 196 847.3 0 196 124 61 847.3 0 61 125 78 847.3 0 78 126 123 847.3 0 123 127 81 847.3 0 81 128 57 847.3 0 57 129 44 847.3 0 44 130 35 847.3 0 35 131 42 847.3 0 42 132 38 847.3 0 38 133 53 847.3 0 53 134 45 847.3 0 45 135 54 847.3 0 54 136 27 847.3 0 27 137 36 847.3 0 36 138 109 847.3 0 109 139 59 847.3 0 59 140 202 847.3 0 202 141 248 847.3 0 248 142 40 847.3 0 40 143 44 847.3 0 44 144 57 847.3 0 57 145 44 847.3 0 44 146 31 847.3 0 31 147 45 847.3 0 45 148 43 847.3 0 43 149 35 847.3 0 35 150 44 847.3 0 44 151 51 847.3 0 51 152 87 847.3 0 87 153 56 847.3 0 56 154 50 847.3 0 50 155 57 847.3 0 57 156 46 847.3 0 46 157 44 847.3 0 44 158 33 847.3 0 33 159 42 847.3 0 42 160 45 847.3 0 45 161 30 847.3 0 30 162 57 847.3 0 57 163 78 847.3 0 78 164 65 847.3 0 65 165 79 847.3 0 79 166 72 847.3 0 72 167 182 847.3 0 182 168 495 847.3 0 495 169 1358 847.3 0 1358 170 94 847.3 0 94 171 256 847.3 0 256 172 243 847.3 0 243 173 390 847.3 0 390 174 66 847.3 0 66 175 45 847.3 0 45 176 37 847.3 0 37 177 40 847.3 0 40 178 27 847.3 0 27 179 47 847.3 0 47 180 80 847.3 0 80 181 109 847.3 0 109 182 137 847.3 0 137 183 122 847.3 0 122 184 81 847.3 0 81 185 75 847.3 0 75 186 78 847.3 0 78 187 71 847.3 0 71 188 75 847.3 0 75 189 108 847.3 0 108 190 92 847.3 0 92 191 90 847.3 0 90 192 52 847.3 0 52 193 72 847.3 0 72 194 71 847.3 0 71 195 62 847.3 0 62 196 51 847.3 0 51 197 75 847.3 0 75 198 87 847.3 0 87 199 113 847.3 0 113 200 318 847.3 0 318 201 475 847.3 0 475 202 489 847.3 0 489 203 225 847.3 0 225 204 55 847.3 0 55 205 105 847.3 0 105 206 127 847.3 0 127 207 49 847.3 0 49 208 61 847.3 0 61 209 36 847.3 0 36 210 53 847.3 0 53 211 88 847.3 0 88 212 56 847.3 0 56 213 68 847.3 0 68 214 85 847.3 0 85 215 119 847.3 0 119 216 157 847.3 0 157 217 117 847.3 0 117 218 117 847.3 0 117 219 119 847.3 0 119 220 97 847.3 0 97 221 105 847.3 0 105 222 76 847.3 0 76 223 106 847.3 0 106 224 144 847.3 0 144 225 213 847.3 0 213 226 345 847.3 0 345 227 512 847.3 0 512 228 3484 847.3 0 3484 229 3406 847.3 0 3406 230 5441 847.3 0 5441 231 420 847.3 0 420 232 126 847.3 0 126 233 55 847.3 0 55 234 55 847.3 0 55 235 58 847.3 0 58 236 69 847.3 0 69 237 62 847.3 0 62 238 77 847.3 0 77 239 84 847.3 0 84 240 84 847.3 0 84 241 61 847.3 0 61 242 56 847.3 0 56 243 111 847.3 0 111 244 114 847.3 0 114 245 124 847.3 0 124 246 217 847.3 0 217 247 228 847.3 0 228 248 207 847.3 0 207 249 138 847.3 0 138 250 118 847.3 0 118 251 75 847.3 0 75 252 85 847.3 0 85 253 72 847.3 0 72 254 157 847.3 0 157 255 127 847.3 0 127 256 444 847.3 0 444 257 432 847.3 0 432 258 383 847.3 0 383 259 153 847.3 0 153 260 158 847.3 0 158 261 897 847.3 0 897 262 1948 847.3 0 1948 263 1574 847.3 0 1574 264 885 847.3 0 885 265 519 847.3 0 519 266 133 847.3 0 133 267 221 847.3 0 221 268 651 847.3 0 651 269 753 847.3 0 753 270 495 847.3 0 495 271 174 847.3 0 174 272 141 847.3 0 141 273 119 847.3 0 119 274 116 847.3 0 116 275 166 847.3 0 166 276 115 847.3 0 115 277 71 847.3 0 71 278 63 847.3 0 63 279 64 847.3 0 64 280 100 847.3 0 100 281 209 847.3 0 209 282 219 847.3 0 219 283 199 847.3 0 199 284 72 847.3 0 72 285 102 847.3 0 102 286 116 847.3 0 116 287 216 847.3 0 216 288 238 847.3 0 238 289 120 847.3 0 120 290 71 847.3 0 71 291 81 847.3 0 81 292 231 847.3 0 231 293 421 847.3 0 421 294 265 847.3 0 265 295 103 847.3 0 103 296 51 847.3 0 51 297 73 847.3 0 73 298 72 847.3 0 72 299 124 847.3 0 124 300 254 847.3 0 254 301 416 847.3 0 416 Finished in 23.31 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 315,043 Reads with adapters: 162,693 (51.6%) Reads that were too short: 122 (0.0%) Reads written (passing filters): 162,571 (51.6%) Total basepairs processed: 90,597,927 bp Total written (filtered): 45,018,296 bp (49.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 162693 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 65 0.0 3 0 0 38 27 16 1426 0.0 3 0 1281 105 40 17 159740 0.0 3 151386 6990 1135 229 18 370 0.0 3 40 284 23 23 19 213 0.0 3 2 1 107 103 20 633 0.0 3 1 0 0 632 21 2 0.0 3 2 22 1 0.0 3 0 0 0 1 23 2 0.0 3 1 0 1 24 3 0.0 3 0 0 0 3 26 1 0.0 3 0 0 0 1 27 2 0.0 3 1 0 0 1 29 2 0.0 3 2 31 15 0.0 3 1 0 0 14 32 2 0.0 3 1 0 0 1 34 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 39 2 0.0 3 0 0 0 2 40 2 0.0 3 1 1 42 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 11 0.0 3 0 0 0 11 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 113 2 0.0 3 1 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 5 0.0 3 0 0 0 5 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 3 0.0 3 0 0 1 2 163 3 0.0 3 0 0 0 3 164 3 0.0 3 0 0 0 3 165 2 0.0 3 0 0 1 1 169 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 187 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 195 1 0.0 3 0 0 0 1 205 9 0.0 3 0 0 0 9 206 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 3 0.0 3 0 0 0 3 235 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 261 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 274 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 6 0.0 3 0 0 0 6 280 17 0.0 3 0 0 0 17 281 14 0.0 3 0 0 0 14 282 15 0.0 3 0 0 0 15 283 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.32 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 162,571 Reads with adapters: 812 (0.5%) Reads written (passing filters): 162,571 (100.0%) Total basepairs processed: 45,018,296 bp Total written (filtered): 45,015,664 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 812 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.3% C: 26.6% G: 18.2% T: 8.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 675 2540.2 0 675 4 100 635.0 0 100 5 27 158.8 0 27 6 8 39.7 0 8 12 2 0.0 1 0 2