This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 183.45 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 1,465,948 (42.2%) Reads that were too short: 69,357 (2.0%) Reads written (passing filters): 1,396,591 (40.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 376,611,588 bp (36.1%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1465948 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1198 847.3 0 1198 7 14245 847.3 0 14245 8 757 847.3 0 757 9 14531 847.3 0 14531 10 1025 847.3 0 1025 11 883 847.3 0 883 12 1668 847.3 0 1668 13 4369 847.3 0 4369 14 761 847.3 0 761 15 1594 847.3 0 1594 16 1073 847.3 0 1073 17 1856 847.3 0 1856 18 5641 847.3 0 5641 19 3292 847.3 0 3292 20 2824 847.3 0 2824 21 670 847.3 0 670 22 1202 847.3 0 1202 23 3258 847.3 0 3258 24 3315 847.3 0 3315 25 6318 847.3 0 6318 26 9149 847.3 0 9149 27 9875 847.3 0 9875 28 18561 847.3 0 18561 29 26055 847.3 0 26055 30 260304 847.3 0 260304 31 342884 847.3 0 342884 32 637647 847.3 0 637647 33 2562 847.3 0 2562 34 228 847.3 0 228 35 543 847.3 0 543 36 479 847.3 0 479 37 369 847.3 0 369 38 55 847.3 0 55 39 40 847.3 0 40 40 30 847.3 0 30 41 59 847.3 0 59 42 30 847.3 0 30 43 31 847.3 0 31 44 52 847.3 0 52 45 36 847.3 0 36 46 35 847.3 0 35 47 51 847.3 0 51 48 35 847.3 0 35 49 37 847.3 0 37 50 39 847.3 0 39 51 37 847.3 0 37 52 35 847.3 0 35 53 63 847.3 0 63 54 416 847.3 0 416 55 836 847.3 0 836 56 455 847.3 0 455 57 29 847.3 0 29 58 29 847.3 0 29 59 17 847.3 0 17 60 32 847.3 0 32 61 25 847.3 0 25 62 25 847.3 0 25 63 13 847.3 0 13 64 19 847.3 0 19 65 19 847.3 0 19 66 28 847.3 0 28 67 40 847.3 0 40 68 33 847.3 0 33 69 54 847.3 0 54 70 55 847.3 0 55 71 88 847.3 0 88 72 58 847.3 0 58 73 45 847.3 0 45 74 37 847.3 0 37 75 23 847.3 0 23 76 30 847.3 0 30 77 29 847.3 0 29 78 48 847.3 0 48 79 42 847.3 0 42 80 67 847.3 0 67 81 41 847.3 0 41 82 44 847.3 0 44 83 43 847.3 0 43 84 51 847.3 0 51 85 46 847.3 0 46 86 38 847.3 0 38 87 98 847.3 0 98 88 95 847.3 0 95 89 84 847.3 0 84 90 43 847.3 0 43 91 62 847.3 0 62 92 39 847.3 0 39 93 22 847.3 0 22 94 33 847.3 0 33 95 51 847.3 0 51 96 73 847.3 0 73 97 49 847.3 0 49 98 41 847.3 0 41 99 44 847.3 0 44 100 49 847.3 0 49 101 51 847.3 0 51 102 232 847.3 0 232 103 535 847.3 0 535 104 2581 847.3 0 2581 105 1624 847.3 0 1624 106 585 847.3 0 585 107 244 847.3 0 244 108 33 847.3 0 33 109 46 847.3 0 46 110 45 847.3 0 45 111 57 847.3 0 57 112 62 847.3 0 62 113 50 847.3 0 50 114 54 847.3 0 54 115 32 847.3 0 32 116 66 847.3 0 66 117 39 847.3 0 39 118 64 847.3 0 64 119 41 847.3 0 41 120 57 847.3 0 57 121 50 847.3 0 50 122 64 847.3 0 64 123 117 847.3 0 117 124 375 847.3 0 375 125 252 847.3 0 252 126 160 847.3 0 160 127 69 847.3 0 69 128 44 847.3 0 44 129 42 847.3 0 42 130 45 847.3 0 45 131 40 847.3 0 40 132 37 847.3 0 37 133 48 847.3 0 48 134 49 847.3 0 49 135 58 847.3 0 58 136 62 847.3 0 62 137 398 847.3 0 398 138 201 847.3 0 201 139 187 847.3 0 187 140 66 847.3 0 66 141 71 847.3 0 71 142 123 847.3 0 123 143 106 847.3 0 106 144 63 847.3 0 63 145 40 847.3 0 40 146 57 847.3 0 57 147 68 847.3 0 68 148 67 847.3 0 67 149 88 847.3 0 88 150 73 847.3 0 73 151 84 847.3 0 84 152 35 847.3 0 35 153 44 847.3 0 44 154 44 847.3 0 44 155 73 847.3 0 73 156 29 847.3 0 29 157 60 847.3 0 60 158 76 847.3 0 76 159 175 847.3 0 175 160 85 847.3 0 85 161 354 847.3 0 354 162 57 847.3 0 57 163 73 847.3 0 73 164 86 847.3 0 86 165 58 847.3 0 58 166 87 847.3 0 87 167 66 847.3 0 66 168 76 847.3 0 76 169 51 847.3 0 51 170 61 847.3 0 61 171 66 847.3 0 66 172 43 847.3 0 43 173 53 847.3 0 53 174 47 847.3 0 47 175 49 847.3 0 49 176 47 847.3 0 47 177 55 847.3 0 55 178 42 847.3 0 42 179 52 847.3 0 52 180 42 847.3 0 42 181 49 847.3 0 49 182 70 847.3 0 70 183 88 847.3 0 88 184 99 847.3 0 99 185 85 847.3 0 85 186 100 847.3 0 100 187 72 847.3 0 72 188 64 847.3 0 64 189 123 847.3 0 123 190 57 847.3 0 57 191 38 847.3 0 38 192 39 847.3 0 39 193 38 847.3 0 38 194 48 847.3 0 48 195 40 847.3 0 40 196 52 847.3 0 52 197 34 847.3 0 34 198 56 847.3 0 56 199 68 847.3 0 68 200 66 847.3 0 66 201 78 847.3 0 78 202 60 847.3 0 60 203 43 847.3 0 43 204 113 847.3 0 113 205 423 847.3 0 423 206 381 847.3 0 381 207 144 847.3 0 144 208 47 847.3 0 47 209 48 847.3 0 48 210 57 847.3 0 57 211 50 847.3 0 50 212 83 847.3 0 83 213 75 847.3 0 75 214 69 847.3 0 69 215 72 847.3 0 72 216 66 847.3 0 66 217 94 847.3 0 94 218 79 847.3 0 79 219 74 847.3 0 74 220 79 847.3 0 79 221 55 847.3 0 55 222 47 847.3 0 47 223 60 847.3 0 60 224 57 847.3 0 57 225 76 847.3 0 76 226 114 847.3 0 114 227 117 847.3 0 117 228 114 847.3 0 114 229 74 847.3 0 74 230 82 847.3 0 82 231 145 847.3 0 145 232 144 847.3 0 144 233 98 847.3 0 98 234 67 847.3 0 67 235 39 847.3 0 39 236 68 847.3 0 68 237 77 847.3 0 77 238 54 847.3 0 54 239 84 847.3 0 84 240 122 847.3 0 122 241 104 847.3 0 104 242 101 847.3 0 101 243 110 847.3 0 110 244 139 847.3 0 139 245 161 847.3 0 161 246 229 847.3 0 229 247 383 847.3 0 383 248 136 847.3 0 136 249 203 847.3 0 203 250 291 847.3 0 291 251 254 847.3 0 254 252 252 847.3 0 252 253 474 847.3 0 474 254 3959 847.3 0 3959 255 3106 847.3 0 3106 256 2185 847.3 0 2185 257 712 847.3 0 712 258 777 847.3 0 777 259 1296 847.3 0 1296 260 21247 847.3 0 21247 261 13929 847.3 0 13929 262 7820 847.3 0 7820 263 126 847.3 0 126 264 72 847.3 0 72 265 58 847.3 0 58 266 67 847.3 0 67 267 105 847.3 0 105 268 152 847.3 0 152 269 169 847.3 0 169 270 120 847.3 0 120 271 154 847.3 0 154 272 187 847.3 0 187 273 465 847.3 0 465 274 322 847.3 0 322 275 590 847.3 0 590 276 686 847.3 0 686 277 1171 847.3 0 1171 278 330 847.3 0 330 279 253 847.3 0 253 280 569 847.3 0 569 281 619 847.3 0 619 282 361 847.3 0 361 283 97 847.3 0 97 284 42 847.3 0 42 285 37 847.3 0 37 286 67 847.3 0 67 287 74 847.3 0 74 288 75 847.3 0 75 289 65 847.3 0 65 290 60 847.3 0 60 291 54 847.3 0 54 292 53 847.3 0 53 293 78 847.3 0 78 294 85 847.3 0 85 295 95 847.3 0 95 296 70 847.3 0 70 297 31 847.3 0 31 298 32 847.3 0 32 299 40 847.3 0 40 300 42 847.3 0 42 301 65 847.3 0 65 Finished in 88.79 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 1,396,591 Reads with adapters: 16,928 (1.2%) Reads that were too short: 706 (0.1%) Reads written (passing filters): 16,222 (1.2%) Total basepairs processed: 376,611,588 bp Total written (filtered): 4,397,379 bp (1.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16928 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 8 2 16 215 0.0 3 0 201 9 5 17 14873 0.0 3 14113 654 82 24 18 82 0.0 3 1 29 4 48 19 48 0.0 3 6 8 18 16 20 580 0.0 3 510 18 6 46 22 2 0.0 3 0 0 0 2 23 3 0.0 3 0 0 0 3 30 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 40 3 0.0 3 0 0 0 3 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 7 0.0 3 0 0 0 7 57 7 0.0 3 0 0 0 7 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 67 40 0.0 3 0 0 2 38 68 49 0.0 3 0 1 1 47 69 26 0.0 3 0 0 0 26 70 5 0.0 3 0 0 0 5 71 5 0.0 3 0 0 0 5 72 12 0.0 3 0 0 1 11 74 1 0.0 3 0 0 0 1 75 5 0.0 3 0 0 1 4 76 2 0.0 3 0 0 0 2 79 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 89 2 0.0 3 0 0 1 1 90 1 0.0 3 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 104 5 0.0 3 0 0 0 5 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 109 1 0.0 3 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 1 112 3 0.0 3 1 0 0 2 114 2 0.0 3 0 0 0 2 115 2 0.0 3 1 0 0 1 117 5 0.0 3 0 0 0 5 118 5 0.0 3 0 0 0 5 119 4 0.0 3 0 0 0 4 120 2 0.0 3 0 0 0 2 121 3 0.0 3 1 0 0 2 122 3 0.0 3 0 0 0 3 125 3 0.0 3 1 1 0 1 127 3 0.0 3 0 0 1 2 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 133 17 0.0 3 0 0 0 17 134 1 0.0 3 0 0 0 1 137 15 0.0 3 0 0 0 15 138 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 1 2 140 2 0.0 3 0 0 0 2 141 20 0.0 3 0 0 0 20 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 151 1 0.0 3 1 153 1 0.0 3 1 155 2 0.0 3 0 0 0 2 156 18 0.0 3 0 0 0 18 157 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 8 0.0 3 1 0 0 7 169 38 0.0 3 0 0 0 38 170 2 0.0 3 0 1 0 1 171 66 0.0 3 0 0 0 66 172 8 0.0 3 0 0 0 8 173 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 2 0.0 3 1 0 0 1 179 2 0.0 3 0 0 0 2 180 8 0.0 3 0 0 0 8 181 9 0.0 3 0 0 0 9 182 55 0.0 3 0 0 0 55 183 14 0.0 3 0 0 1 13 184 4 0.0 3 0 0 0 4 188 2 0.0 3 0 1 0 1 189 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 19 0.0 3 0 0 0 19 199 11 0.0 3 0 0 0 11 200 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 4 0.0 3 0 0 0 4 215 11 0.0 3 0 0 0 11 216 1 0.0 3 0 0 0 1 220 5 0.0 3 0 0 0 5 221 4 0.0 3 0 0 0 4 224 3 0.0 3 0 1 0 2 228 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 12 0.0 3 0 0 0 12 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 255 13 0.0 3 0 0 0 13 256 49 0.0 3 0 0 0 49 257 89 0.0 3 0 0 0 89 258 106 0.0 3 0 0 1 105 259 107 0.0 3 0 0 1 106 260 28 0.0 3 0 0 1 27 261 4 0.0 3 0 0 0 4 262 6 0.0 3 0 0 0 6 263 4 0.0 3 0 0 0 4 264 4 0.0 3 0 0 0 4 265 4 0.0 3 0 0 0 4 266 4 0.0 3 0 0 0 4 267 1 0.0 3 0 0 0 1 268 2 0.0 3 0 0 0 2 269 5 0.0 3 0 0 0 5 270 3 0.0 3 0 0 0 3 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 274 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 16,222 Reads with adapters: 758 (4.7%) Reads written (passing filters): 16,222 (100.0%) Total basepairs processed: 4,397,379 bp Total written (filtered): 4,395,098 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 758 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.0% C: 9.2% G: 12.4% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 751 253.5 0 751 4 7 63.4 0 7