This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 163.59 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 72,286 (2.1%) Reads that were too short: 16,565 (0.5%) Reads written (passing filters): 55,721 (1.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 13,936,629 bp (1.3%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 72286 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1486 847.3 0 1486 7 13147 847.3 0 13147 8 537 847.3 0 537 9 15147 847.3 0 15147 10 115 847.3 0 115 11 96 847.3 0 96 12 71 847.3 0 71 13 76 847.3 0 76 14 86 847.3 0 86 15 91 847.3 0 91 16 96 847.3 0 96 17 82 847.3 0 82 18 79 847.3 0 79 19 80 847.3 0 80 20 170 847.3 0 170 21 93 847.3 0 93 22 119 847.3 0 119 23 389 847.3 0 389 24 534 847.3 0 534 25 1052 847.3 0 1052 26 51 847.3 0 51 27 41 847.3 0 41 28 69 847.3 0 69 29 68 847.3 0 68 30 66 847.3 0 66 31 54 847.3 0 54 32 66 847.3 0 66 33 64 847.3 0 64 34 82 847.3 0 82 35 83 847.3 0 83 36 62 847.3 0 62 37 92 847.3 0 92 38 63 847.3 0 63 39 72 847.3 0 72 40 58 847.3 0 58 41 65 847.3 0 65 42 56 847.3 0 56 43 63 847.3 0 63 44 62 847.3 0 62 45 65 847.3 0 65 46 71 847.3 0 71 47 63 847.3 0 63 48 59 847.3 0 59 49 66 847.3 0 66 50 59 847.3 0 59 51 57 847.3 0 57 52 58 847.3 0 58 53 46 847.3 0 46 54 67 847.3 0 67 55 60 847.3 0 60 56 70 847.3 0 70 57 58 847.3 0 58 58 63 847.3 0 63 59 58 847.3 0 58 60 64 847.3 0 64 61 62 847.3 0 62 62 50 847.3 0 50 63 44 847.3 0 44 64 67 847.3 0 67 65 69 847.3 0 69 66 69 847.3 0 69 67 55 847.3 0 55 68 45 847.3 0 45 69 62 847.3 0 62 70 79 847.3 0 79 71 74 847.3 0 74 72 66 847.3 0 66 73 66 847.3 0 66 74 108 847.3 0 108 75 153 847.3 0 153 76 51 847.3 0 51 77 53 847.3 0 53 78 55 847.3 0 55 79 33 847.3 0 33 80 45 847.3 0 45 81 65 847.3 0 65 82 75 847.3 0 75 83 70 847.3 0 70 84 104 847.3 0 104 85 122 847.3 0 122 86 102 847.3 0 102 87 150 847.3 0 150 88 664 847.3 0 664 89 547 847.3 0 547 90 491 847.3 0 491 91 40 847.3 0 40 92 53 847.3 0 53 93 67 847.3 0 67 94 88 847.3 0 88 95 123 847.3 0 123 96 404 847.3 0 404 97 234 847.3 0 234 98 113 847.3 0 113 99 71 847.3 0 71 100 120 847.3 0 120 101 515 847.3 0 515 102 1026 847.3 0 1026 103 1515 847.3 0 1515 104 109 847.3 0 109 105 69 847.3 0 69 106 56 847.3 0 56 107 34 847.3 0 34 108 38 847.3 0 38 109 52 847.3 0 52 110 87 847.3 0 87 111 90 847.3 0 90 112 75 847.3 0 75 113 144 847.3 0 144 114 155 847.3 0 155 115 88 847.3 0 88 116 75 847.3 0 75 117 102 847.3 0 102 118 72 847.3 0 72 119 66 847.3 0 66 120 88 847.3 0 88 121 66 847.3 0 66 122 93 847.3 0 93 123 92 847.3 0 92 124 131 847.3 0 131 125 116 847.3 0 116 126 256 847.3 0 256 127 2280 847.3 0 2280 128 908 847.3 0 908 129 1363 847.3 0 1363 130 112 847.3 0 112 131 89 847.3 0 89 132 85 847.3 0 85 133 85 847.3 0 85 134 88 847.3 0 88 135 75 847.3 0 75 136 54 847.3 0 54 137 86 847.3 0 86 138 68 847.3 0 68 139 67 847.3 0 67 140 66 847.3 0 66 141 101 847.3 0 101 142 68 847.3 0 68 143 70 847.3 0 70 144 176 847.3 0 176 145 77 847.3 0 77 146 126 847.3 0 126 147 80 847.3 0 80 148 61 847.3 0 61 149 97 847.3 0 97 150 53 847.3 0 53 151 55 847.3 0 55 152 71 847.3 0 71 153 50 847.3 0 50 154 49 847.3 0 49 155 54 847.3 0 54 156 71 847.3 0 71 157 66 847.3 0 66 158 57 847.3 0 57 159 56 847.3 0 56 160 52 847.3 0 52 161 78 847.3 0 78 162 57 847.3 0 57 163 58 847.3 0 58 164 43 847.3 0 43 165 36 847.3 0 36 166 47 847.3 0 47 167 56 847.3 0 56 168 59 847.3 0 59 169 85 847.3 0 85 170 52 847.3 0 52 171 51 847.3 0 51 172 65 847.3 0 65 173 33 847.3 0 33 174 39 847.3 0 39 175 50 847.3 0 50 176 113 847.3 0 113 177 92 847.3 0 92 178 101 847.3 0 101 179 43 847.3 0 43 180 48 847.3 0 48 181 60 847.3 0 60 182 84 847.3 0 84 183 74 847.3 0 74 184 52 847.3 0 52 185 55 847.3 0 55 186 47 847.3 0 47 187 59 847.3 0 59 188 46 847.3 0 46 189 50 847.3 0 50 190 50 847.3 0 50 191 40 847.3 0 40 192 56 847.3 0 56 193 98 847.3 0 98 194 103 847.3 0 103 195 114 847.3 0 114 196 128 847.3 0 128 197 90 847.3 0 90 198 73 847.3 0 73 199 42 847.3 0 42 200 138 847.3 0 138 201 154 847.3 0 154 202 159 847.3 0 159 203 98 847.3 0 98 204 65 847.3 0 65 205 64 847.3 0 64 206 56 847.3 0 56 207 72 847.3 0 72 208 117 847.3 0 117 209 133 847.3 0 133 210 91 847.3 0 91 211 79 847.3 0 79 212 61 847.3 0 61 213 80 847.3 0 80 214 63 847.3 0 63 215 79 847.3 0 79 216 67 847.3 0 67 217 83 847.3 0 83 218 104 847.3 0 104 219 84 847.3 0 84 220 62 847.3 0 62 221 68 847.3 0 68 222 61 847.3 0 61 223 100 847.3 0 100 224 66 847.3 0 66 225 54 847.3 0 54 226 64 847.3 0 64 227 58 847.3 0 58 228 38 847.3 0 38 229 80 847.3 0 80 230 79 847.3 0 79 231 74 847.3 0 74 232 83 847.3 0 83 233 60 847.3 0 60 234 184 847.3 0 184 235 216 847.3 0 216 236 88 847.3 0 88 237 79 847.3 0 79 238 122 847.3 0 122 239 145 847.3 0 145 240 250 847.3 0 250 241 205 847.3 0 205 242 184 847.3 0 184 243 98 847.3 0 98 244 64 847.3 0 64 245 91 847.3 0 91 246 90 847.3 0 90 247 86 847.3 0 86 248 57 847.3 0 57 249 89 847.3 0 89 250 80 847.3 0 80 251 65 847.3 0 65 252 91 847.3 0 91 253 61 847.3 0 61 254 101 847.3 0 101 255 303 847.3 0 303 256 107 847.3 0 107 257 120 847.3 0 120 258 151 847.3 0 151 259 1643 847.3 0 1643 260 2185 847.3 0 2185 261 1549 847.3 0 1549 262 304 847.3 0 304 263 65 847.3 0 65 264 59 847.3 0 59 265 43 847.3 0 43 266 57 847.3 0 57 267 63 847.3 0 63 268 81 847.3 0 81 269 70 847.3 0 70 270 55 847.3 0 55 271 49 847.3 0 49 272 52 847.3 0 52 273 79 847.3 0 79 274 59 847.3 0 59 275 69 847.3 0 69 276 75 847.3 0 75 277 72 847.3 0 72 278 94 847.3 0 94 279 743 847.3 0 743 280 1494 847.3 0 1494 281 208 847.3 0 208 282 79 847.3 0 79 283 62 847.3 0 62 284 74 847.3 0 74 285 53 847.3 0 53 286 71 847.3 0 71 287 54 847.3 0 54 288 59 847.3 0 59 289 70 847.3 0 70 290 54 847.3 0 54 291 96 847.3 0 96 292 124 847.3 0 124 293 140 847.3 0 140 294 125 847.3 0 125 295 125 847.3 0 125 296 110 847.3 0 110 297 121 847.3 0 121 298 74 847.3 0 74 299 101 847.3 0 101 300 87 847.3 0 87 301 89 847.3 0 89 Finished in 3.91 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 55,721 Reads with adapters: 16,076 (28.9%) Reads that were too short: 58 (0.1%) Reads written (passing filters): 16,018 (28.7%) Total basepairs processed: 13,936,629 bp Total written (filtered): 4,372,240 bp (31.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16076 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 18 0.0 3 0 0 11 7 16 215 0.0 3 0 191 17 7 17 15447 0.0 3 14615 708 105 19 18 41 0.0 3 0 30 5 6 19 29 0.0 3 7 0 14 8 20 49 0.0 3 3 0 0 46 26 10 0.0 3 0 0 0 10 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 4 0.0 3 0 0 0 4 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 2 41 1 0.0 3 0 0 0 1 44 9 0.0 3 0 0 0 9 47 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 1 56 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 62 4 0.0 3 0 0 0 4 64 103 0.0 3 0 0 0 103 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 12 0.0 3 0 0 1 11 68 1 0.0 3 0 0 1 70 1 0.0 3 0 0 0 1 75 6 0.0 3 0 0 0 6 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 4 0.0 3 0 0 0 4 102 1 0.0 3 0 0 0 1 103 11 0.0 3 0 0 1 10 104 17 0.0 3 0 0 0 17 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 1 2 161 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 1 205 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.94 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 16,018 Reads with adapters: 749 (4.7%) Reads written (passing filters): 16,018 (100.0%) Total basepairs processed: 4,372,240 bp Total written (filtered): 4,369,970 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 749 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.6% C: 10.9% G: 19.9% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 732 250.3 0 732 4 14 62.6 0 14 5 1 15.6 0 1 6 1 3.9 0 1 7 1 1.0 0 1