This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 164.51 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 71,940 (2.1%) Reads that were too short: 13,073 (0.4%) Reads written (passing filters): 58,867 (1.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 15,513,368 bp (1.5%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 71940 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 358 847.3 0 358 7 768 847.3 0 768 8 24420 847.3 0 24420 9 16644 847.3 0 16644 10 64 847.3 0 64 11 91 847.3 0 91 12 16 847.3 0 16 13 32 847.3 0 32 14 46 847.3 0 46 15 72 847.3 0 72 16 39 847.3 0 39 17 42 847.3 0 42 18 58 847.3 0 58 19 76 847.3 0 76 20 107 847.3 0 107 21 22 847.3 0 22 22 16 847.3 0 16 23 33 847.3 0 33 24 38 847.3 0 38 25 25 847.3 0 25 26 16 847.3 0 16 27 39 847.3 0 39 28 109 847.3 0 109 29 183 847.3 0 183 30 65 847.3 0 65 31 27 847.3 0 27 32 29 847.3 0 29 33 19 847.3 0 19 34 31 847.3 0 31 35 38 847.3 0 38 36 45 847.3 0 45 37 37 847.3 0 37 38 25 847.3 0 25 39 18 847.3 0 18 40 33 847.3 0 33 41 28 847.3 0 28 42 24 847.3 0 24 43 60 847.3 0 60 44 118 847.3 0 118 45 595 847.3 0 595 46 496 847.3 0 496 47 189 847.3 0 189 48 17 847.3 0 17 49 19 847.3 0 19 50 18 847.3 0 18 51 18 847.3 0 18 52 19 847.3 0 19 53 26 847.3 0 26 54 16 847.3 0 16 55 16 847.3 0 16 56 19 847.3 0 19 57 15 847.3 0 15 58 23 847.3 0 23 59 15 847.3 0 15 60 30 847.3 0 30 61 15 847.3 0 15 62 17 847.3 0 17 63 17 847.3 0 17 64 22 847.3 0 22 65 19 847.3 0 19 66 16 847.3 0 16 67 15 847.3 0 15 68 25 847.3 0 25 69 101 847.3 0 101 70 44 847.3 0 44 71 102 847.3 0 102 72 133 847.3 0 133 73 129 847.3 0 129 74 116 847.3 0 116 75 58 847.3 0 58 76 39 847.3 0 39 77 33 847.3 0 33 78 75 847.3 0 75 79 103 847.3 0 103 80 504 847.3 0 504 81 172 847.3 0 172 82 99 847.3 0 99 83 153 847.3 0 153 84 195 847.3 0 195 85 77 847.3 0 77 86 44 847.3 0 44 87 21 847.3 0 21 88 36 847.3 0 36 89 29 847.3 0 29 90 41 847.3 0 41 91 39 847.3 0 39 92 39 847.3 0 39 93 41 847.3 0 41 94 178 847.3 0 178 95 259 847.3 0 259 96 786 847.3 0 786 97 478 847.3 0 478 98 28 847.3 0 28 99 34 847.3 0 34 100 22 847.3 0 22 101 22 847.3 0 22 102 23 847.3 0 23 103 35 847.3 0 35 104 25 847.3 0 25 105 15 847.3 0 15 106 26 847.3 0 26 107 31 847.3 0 31 108 37 847.3 0 37 109 32 847.3 0 32 110 28 847.3 0 28 111 43 847.3 0 43 112 65 847.3 0 65 113 591 847.3 0 591 114 439 847.3 0 439 115 213 847.3 0 213 116 125 847.3 0 125 117 54 847.3 0 54 118 31 847.3 0 31 119 27 847.3 0 27 120 52 847.3 0 52 121 46 847.3 0 46 122 45 847.3 0 45 123 69 847.3 0 69 124 109 847.3 0 109 125 41 847.3 0 41 126 52 847.3 0 52 127 45 847.3 0 45 128 59 847.3 0 59 129 60 847.3 0 60 130 62 847.3 0 62 131 72 847.3 0 72 132 67 847.3 0 67 133 94 847.3 0 94 134 55 847.3 0 55 135 156 847.3 0 156 136 257 847.3 0 257 137 487 847.3 0 487 138 251 847.3 0 251 139 138 847.3 0 138 140 225 847.3 0 225 141 73 847.3 0 73 142 124 847.3 0 124 143 138 847.3 0 138 144 89 847.3 0 89 145 70 847.3 0 70 146 36 847.3 0 36 147 49 847.3 0 49 148 37 847.3 0 37 149 66 847.3 0 66 150 62 847.3 0 62 151 32 847.3 0 32 152 36 847.3 0 36 153 33 847.3 0 33 154 42 847.3 0 42 155 52 847.3 0 52 156 55 847.3 0 55 157 45 847.3 0 45 158 39 847.3 0 39 159 30 847.3 0 30 160 29 847.3 0 29 161 31 847.3 0 31 162 70 847.3 0 70 163 84 847.3 0 84 164 100 847.3 0 100 165 60 847.3 0 60 166 53 847.3 0 53 167 104 847.3 0 104 168 206 847.3 0 206 169 123 847.3 0 123 170 78 847.3 0 78 171 49 847.3 0 49 172 34 847.3 0 34 173 52 847.3 0 52 174 50 847.3 0 50 175 35 847.3 0 35 176 30 847.3 0 30 177 30 847.3 0 30 178 53 847.3 0 53 179 138 847.3 0 138 180 85 847.3 0 85 181 56 847.3 0 56 182 53 847.3 0 53 183 98 847.3 0 98 184 251 847.3 0 251 185 426 847.3 0 426 186 344 847.3 0 344 187 178 847.3 0 178 188 72 847.3 0 72 189 67 847.3 0 67 190 59 847.3 0 59 191 66 847.3 0 66 192 35 847.3 0 35 193 58 847.3 0 58 194 40 847.3 0 40 195 47 847.3 0 47 196 41 847.3 0 41 197 37 847.3 0 37 198 42 847.3 0 42 199 85 847.3 0 85 200 56 847.3 0 56 201 54 847.3 0 54 202 48 847.3 0 48 203 93 847.3 0 93 204 401 847.3 0 401 205 481 847.3 0 481 206 319 847.3 0 319 207 126 847.3 0 126 208 84 847.3 0 84 209 110 847.3 0 110 210 76 847.3 0 76 211 66 847.3 0 66 212 94 847.3 0 94 213 60 847.3 0 60 214 51 847.3 0 51 215 101 847.3 0 101 216 80 847.3 0 80 217 78 847.3 0 78 218 68 847.3 0 68 219 70 847.3 0 70 220 62 847.3 0 62 221 64 847.3 0 64 222 82 847.3 0 82 223 121 847.3 0 121 224 314 847.3 0 314 225 1835 847.3 0 1835 226 2521 847.3 0 2521 227 1176 847.3 0 1176 228 267 847.3 0 267 229 41 847.3 0 41 230 42 847.3 0 42 231 38 847.3 0 38 232 50 847.3 0 50 233 44 847.3 0 44 234 82 847.3 0 82 235 130 847.3 0 130 236 134 847.3 0 134 237 54 847.3 0 54 238 40 847.3 0 40 239 103 847.3 0 103 240 89 847.3 0 89 241 48 847.3 0 48 242 42 847.3 0 42 243 44 847.3 0 44 244 62 847.3 0 62 245 40 847.3 0 40 246 53 847.3 0 53 247 46 847.3 0 46 248 57 847.3 0 57 249 45 847.3 0 45 250 31 847.3 0 31 251 23 847.3 0 23 252 30 847.3 0 30 253 47 847.3 0 47 254 57 847.3 0 57 255 49 847.3 0 49 256 26 847.3 0 26 257 50 847.3 0 50 258 44 847.3 0 44 259 53 847.3 0 53 260 53 847.3 0 53 261 45 847.3 0 45 262 43 847.3 0 43 263 51 847.3 0 51 264 202 847.3 0 202 265 144 847.3 0 144 266 80 847.3 0 80 267 15 847.3 0 15 268 31 847.3 0 31 269 57 847.3 0 57 270 34 847.3 0 34 271 28 847.3 0 28 272 32 847.3 0 32 273 47 847.3 0 47 274 46 847.3 0 46 275 56 847.3 0 56 276 38 847.3 0 38 277 44 847.3 0 44 278 25 847.3 0 25 279 59 847.3 0 59 280 89 847.3 0 89 281 49 847.3 0 49 282 41 847.3 0 41 283 32 847.3 0 32 284 29 847.3 0 29 285 31 847.3 0 31 286 44 847.3 0 44 287 32 847.3 0 32 288 36 847.3 0 36 289 37 847.3 0 37 290 53 847.3 0 53 291 48 847.3 0 48 292 52 847.3 0 52 293 79 847.3 0 79 294 86 847.3 0 86 295 130 847.3 0 130 296 62 847.3 0 62 297 103 847.3 0 103 298 77 847.3 0 77 299 65 847.3 0 65 300 41 847.3 0 41 301 185 847.3 0 185 Finished in 4.31 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 58,867 Reads with adapters: 24,299 (41.3%) Reads that were too short: 63 (0.1%) Reads written (passing filters): 24,236 (41.2%) Total basepairs processed: 15,513,368 bp Total written (filtered): 6,667,595 bp (43.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24299 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 23 0.0 3 0 0 12 11 16 268 0.0 3 0 228 20 20 17 23594 0.0 3 22355 1046 158 35 18 98 0.0 3 9 57 6 26 19 41 0.0 3 0 1 29 11 20 58 0.0 3 1 0 0 57 24 6 0.0 3 0 0 0 6 27 4 0.0 3 1 1 0 2 28 5 0.0 3 0 0 0 5 30 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 1 1 35 2 0.0 3 0 0 0 2 38 4 0.0 3 0 0 0 4 39 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 52 5 0.0 3 0 0 0 5 54 4 0.0 3 0 0 0 4 55 2 0.0 3 0 0 0 2 56 7 0.0 3 0 0 1 6 57 3 0.0 3 0 0 0 3 58 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 5 0.0 3 0 0 0 5 78 27 0.0 3 0 0 0 27 81 7 0.0 3 0 0 1 6 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 3 0.0 3 0 0 0 3 130 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 10 0.0 3 0 0 0 10 147 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 11 0.0 3 0 0 1 10 164 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 238 4 0.0 3 0 0 0 4 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 2 0.0 3 0 0 1 1 266 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.37 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 24,236 Reads with adapters: 227 (0.9%) Reads written (passing filters): 24,236 (100.0%) Total basepairs processed: 6,667,595 bp Total written (filtered): 6,666,884 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 227 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.1% C: 15.4% G: 12.3% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 204 378.7 0 204 4 18 94.7 0 18 5 4 23.7 0 4 7 1 1.5 0 1