This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 162.30 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 65,164 (1.9%) Reads that were too short: 10,221 (0.3%) Reads written (passing filters): 54,943 (1.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 14,775,603 bp (1.4%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 65164 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 220 847.3 0 220 7 431 847.3 0 431 8 22643 847.3 0 22643 9 19521 847.3 0 19521 10 39 847.3 0 39 11 44 847.3 0 44 12 8 847.3 0 8 13 22 847.3 0 22 14 9 847.3 0 9 15 12 847.3 0 12 16 13 847.3 0 13 17 21 847.3 0 21 18 24 847.3 0 24 19 30 847.3 0 30 20 50 847.3 0 50 21 15 847.3 0 15 22 14 847.3 0 14 23 15 847.3 0 15 24 14 847.3 0 14 25 14 847.3 0 14 26 15 847.3 0 15 27 7 847.3 0 7 28 28 847.3 0 28 29 34 847.3 0 34 30 32 847.3 0 32 31 16 847.3 0 16 32 17 847.3 0 17 33 14 847.3 0 14 34 28 847.3 0 28 35 46 847.3 0 46 36 25 847.3 0 25 37 27 847.3 0 27 38 11 847.3 0 11 39 22 847.3 0 22 40 13 847.3 0 13 41 16 847.3 0 16 42 19 847.3 0 19 43 39 847.3 0 39 44 57 847.3 0 57 45 457 847.3 0 457 46 303 847.3 0 303 47 128 847.3 0 128 48 19 847.3 0 19 49 11 847.3 0 11 50 17 847.3 0 17 51 17 847.3 0 17 52 19 847.3 0 19 53 12 847.3 0 12 54 18 847.3 0 18 55 16 847.3 0 16 56 17 847.3 0 17 57 21 847.3 0 21 58 18 847.3 0 18 59 20 847.3 0 20 60 16 847.3 0 16 61 12 847.3 0 12 62 14 847.3 0 14 63 12 847.3 0 12 64 17 847.3 0 17 65 16 847.3 0 16 66 15 847.3 0 15 67 17 847.3 0 17 68 21 847.3 0 21 69 80 847.3 0 80 70 14 847.3 0 14 71 36 847.3 0 36 72 42 847.3 0 42 73 68 847.3 0 68 74 11 847.3 0 11 75 13 847.3 0 13 76 57 847.3 0 57 77 84 847.3 0 84 78 58 847.3 0 58 79 47 847.3 0 47 80 50 847.3 0 50 81 50 847.3 0 50 82 40 847.3 0 40 83 25 847.3 0 25 84 43 847.3 0 43 85 66 847.3 0 66 86 44 847.3 0 44 87 41 847.3 0 41 88 64 847.3 0 64 89 43 847.3 0 43 90 49 847.3 0 49 91 71 847.3 0 71 92 69 847.3 0 69 93 66 847.3 0 66 94 205 847.3 0 205 95 267 847.3 0 267 96 813 847.3 0 813 97 626 847.3 0 626 98 39 847.3 0 39 99 32 847.3 0 32 100 35 847.3 0 35 101 30 847.3 0 30 102 22 847.3 0 22 103 14 847.3 0 14 104 23 847.3 0 23 105 15 847.3 0 15 106 29 847.3 0 29 107 36 847.3 0 36 108 46 847.3 0 46 109 28 847.3 0 28 110 26 847.3 0 26 111 31 847.3 0 31 112 51 847.3 0 51 113 314 847.3 0 314 114 253 847.3 0 253 115 141 847.3 0 141 116 105 847.3 0 105 117 35 847.3 0 35 118 19 847.3 0 19 119 36 847.3 0 36 120 42 847.3 0 42 121 33 847.3 0 33 122 39 847.3 0 39 123 54 847.3 0 54 124 79 847.3 0 79 125 35 847.3 0 35 126 32 847.3 0 32 127 39 847.3 0 39 128 41 847.3 0 41 129 54 847.3 0 54 130 44 847.3 0 44 131 54 847.3 0 54 132 43 847.3 0 43 133 81 847.3 0 81 134 48 847.3 0 48 135 173 847.3 0 173 136 249 847.3 0 249 137 402 847.3 0 402 138 215 847.3 0 215 139 104 847.3 0 104 140 191 847.3 0 191 141 47 847.3 0 47 142 111 847.3 0 111 143 114 847.3 0 114 144 56 847.3 0 56 145 53 847.3 0 53 146 40 847.3 0 40 147 30 847.3 0 30 148 29 847.3 0 29 149 47 847.3 0 47 150 38 847.3 0 38 151 29 847.3 0 29 152 41 847.3 0 41 153 30 847.3 0 30 154 44 847.3 0 44 155 42 847.3 0 42 156 39 847.3 0 39 157 40 847.3 0 40 158 35 847.3 0 35 159 25 847.3 0 25 160 24 847.3 0 24 161 29 847.3 0 29 162 55 847.3 0 55 163 81 847.3 0 81 164 73 847.3 0 73 165 42 847.3 0 42 166 30 847.3 0 30 167 105 847.3 0 105 168 189 847.3 0 189 169 81 847.3 0 81 170 41 847.3 0 41 171 35 847.3 0 35 172 39 847.3 0 39 173 41 847.3 0 41 174 39 847.3 0 39 175 26 847.3 0 26 176 30 847.3 0 30 177 28 847.3 0 28 178 46 847.3 0 46 179 92 847.3 0 92 180 61 847.3 0 61 181 44 847.3 0 44 182 45 847.3 0 45 183 57 847.3 0 57 184 165 847.3 0 165 185 243 847.3 0 243 186 207 847.3 0 207 187 103 847.3 0 103 188 45 847.3 0 45 189 45 847.3 0 45 190 50 847.3 0 50 191 48 847.3 0 48 192 31 847.3 0 31 193 37 847.3 0 37 194 38 847.3 0 38 195 43 847.3 0 43 196 32 847.3 0 32 197 40 847.3 0 40 198 50 847.3 0 50 199 54 847.3 0 54 200 41 847.3 0 41 201 36 847.3 0 36 202 34 847.3 0 34 203 64 847.3 0 64 204 196 847.3 0 196 205 207 847.3 0 207 206 130 847.3 0 130 207 69 847.3 0 69 208 56 847.3 0 56 209 72 847.3 0 72 210 64 847.3 0 64 211 50 847.3 0 50 212 51 847.3 0 51 213 61 847.3 0 61 214 34 847.3 0 34 215 70 847.3 0 70 216 89 847.3 0 89 217 67 847.3 0 67 218 62 847.3 0 62 219 43 847.3 0 43 220 50 847.3 0 50 221 61 847.3 0 61 222 59 847.3 0 59 223 113 847.3 0 113 224 282 847.3 0 282 225 1486 847.3 0 1486 226 2260 847.3 0 2260 227 999 847.3 0 999 228 280 847.3 0 280 229 30 847.3 0 30 230 29 847.3 0 29 231 40 847.3 0 40 232 38 847.3 0 38 233 42 847.3 0 42 234 46 847.3 0 46 235 75 847.3 0 75 236 74 847.3 0 74 237 39 847.3 0 39 238 20 847.3 0 20 239 48 847.3 0 48 240 50 847.3 0 50 241 20 847.3 0 20 242 57 847.3 0 57 243 42 847.3 0 42 244 37 847.3 0 37 245 38 847.3 0 38 246 38 847.3 0 38 247 41 847.3 0 41 248 49 847.3 0 49 249 43 847.3 0 43 250 35 847.3 0 35 251 26 847.3 0 26 252 26 847.3 0 26 253 55 847.3 0 55 254 49 847.3 0 49 255 39 847.3 0 39 256 37 847.3 0 37 257 40 847.3 0 40 258 36 847.3 0 36 259 50 847.3 0 50 260 48 847.3 0 48 261 43 847.3 0 43 262 26 847.3 0 26 263 40 847.3 0 40 264 89 847.3 0 89 265 73 847.3 0 73 266 45 847.3 0 45 267 18 847.3 0 18 268 35 847.3 0 35 269 50 847.3 0 50 270 29 847.3 0 29 271 23 847.3 0 23 272 29 847.3 0 29 273 39 847.3 0 39 274 41 847.3 0 41 275 30 847.3 0 30 276 23 847.3 0 23 277 29 847.3 0 29 278 28 847.3 0 28 279 41 847.3 0 41 280 70 847.3 0 70 281 51 847.3 0 51 282 22 847.3 0 22 283 38 847.3 0 38 284 43 847.3 0 43 285 29 847.3 0 29 286 43 847.3 0 43 287 31 847.3 0 31 288 37 847.3 0 37 289 39 847.3 0 39 290 33 847.3 0 33 291 40 847.3 0 40 292 36 847.3 0 36 293 62 847.3 0 62 294 64 847.3 0 64 295 158 847.3 0 158 296 45 847.3 0 45 297 53 847.3 0 53 298 56 847.3 0 56 299 45 847.3 0 45 300 24 847.3 0 24 301 125 847.3 0 125 Finished in 4.27 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 54,943 Reads with adapters: 20,455 (37.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 20,437 (37.2%) Total basepairs processed: 14,775,603 bp Total written (filtered): 5,550,181 bp (37.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20455 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 9 1 19 259 0.0 3 0 256 3 20 19990 0.0 4 19644 331 12 2 1 21 64 0.0 4 19 43 1 1 22 4 0.0 4 1 0 3 23 19 0.0 4 0 0 0 16 3 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 89 6 0.0 4 0 0 0 0 6 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 62 0.0 4 0 0 0 0 62 139 1 0.0 4 1 140 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 172 3 0.0 4 0 0 0 0 3 177 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 1 211 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 252 1 0.0 4 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 20,437 Reads with adapters: 2,915 (14.3%) Reads written (passing filters): 20,437 (100.0%) Total basepairs processed: 5,550,181 bp Total written (filtered): 5,512,931 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2915 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.0% G: 94.6% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 319.3 0 7 4 14 79.8 0 14 5 2 20.0 0 2 6 38 5.0 0 38 9 1627 0.1 0 38 1589 10 1064 0.0 1 43 1021 11 119 0.0 1 3 116 12 1 0.0 1 0 1 13 1 0.0 1 1 24 1 0.0 1 1 150 1 0.0 1 0 1 189 1 0.0 1 0 1 208 1 0.0 1 0 1 223 9 0.0 1 6 3 224 2 0.0 1 1 1 270 25 0.0 1 25 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.