This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.69 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 665,115 (19.2%) Reads that were too short: 21,487 (0.6%) Reads written (passing filters): 643,628 (18.5%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 109,019,575 bp (10.4%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 665115 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2476 847.3 0 2476 7 13378 847.3 0 13378 8 10519 847.3 0 10519 9 193 847.3 0 193 10 288 847.3 0 288 11 122 847.3 0 122 12 118 847.3 0 118 13 94 847.3 0 94 14 89 847.3 0 89 15 116 847.3 0 116 16 79 847.3 0 79 17 72 847.3 0 72 18 87 847.3 0 87 19 94 847.3 0 94 20 88 847.3 0 88 21 84 847.3 0 84 22 84 847.3 0 84 23 106 847.3 0 106 24 132 847.3 0 132 25 229 847.3 0 229 26 161 847.3 0 161 27 147 847.3 0 147 28 109 847.3 0 109 29 92 847.3 0 92 30 107 847.3 0 107 31 66 847.3 0 66 32 63 847.3 0 63 33 81 847.3 0 81 34 87 847.3 0 87 35 111 847.3 0 111 36 131 847.3 0 131 37 125 847.3 0 125 38 80 847.3 0 80 39 84 847.3 0 84 40 162 847.3 0 162 41 72 847.3 0 72 42 116 847.3 0 116 43 128 847.3 0 128 44 89 847.3 0 89 45 90 847.3 0 90 46 84 847.3 0 84 47 75 847.3 0 75 48 81 847.3 0 81 49 90 847.3 0 90 50 81 847.3 0 81 51 93 847.3 0 93 52 78 847.3 0 78 53 154 847.3 0 154 54 87 847.3 0 87 55 109 847.3 0 109 56 119 847.3 0 119 57 92 847.3 0 92 58 95 847.3 0 95 59 70 847.3 0 70 60 100 847.3 0 100 61 155 847.3 0 155 62 137 847.3 0 137 63 145 847.3 0 145 64 111 847.3 0 111 65 123 847.3 0 123 66 117 847.3 0 117 67 124 847.3 0 124 68 147 847.3 0 147 69 151 847.3 0 151 70 169 847.3 0 169 71 131 847.3 0 131 72 583 847.3 0 583 73 348 847.3 0 348 74 1913 847.3 0 1913 75 686 847.3 0 686 76 265 847.3 0 265 77 702 847.3 0 702 78 385 847.3 0 385 79 450 847.3 0 450 80 441 847.3 0 441 81 1824 847.3 0 1824 82 711 847.3 0 711 83 860 847.3 0 860 84 574 847.3 0 574 85 374 847.3 0 374 86 377 847.3 0 377 87 221 847.3 0 221 88 149 847.3 0 149 89 150 847.3 0 150 90 201 847.3 0 201 91 149 847.3 0 149 92 152 847.3 0 152 93 223 847.3 0 223 94 297 847.3 0 297 95 201 847.3 0 201 96 219 847.3 0 219 97 231 847.3 0 231 98 190 847.3 0 190 99 233 847.3 0 233 100 280 847.3 0 280 101 378 847.3 0 378 102 499 847.3 0 499 103 561 847.3 0 561 104 803 847.3 0 803 105 1196 847.3 0 1196 106 471 847.3 0 471 107 556 847.3 0 556 108 1005 847.3 0 1005 109 626 847.3 0 626 110 511 847.3 0 511 111 1086 847.3 0 1086 112 840 847.3 0 840 113 1052 847.3 0 1052 114 969 847.3 0 969 115 1395 847.3 0 1395 116 1952 847.3 0 1952 117 3104 847.3 0 3104 118 51448 847.3 0 51448 119 45974 847.3 0 45974 120 25734 847.3 0 25734 121 5989 847.3 0 5989 122 5625 847.3 0 5625 123 4073 847.3 0 4073 124 602 847.3 0 602 125 606 847.3 0 606 126 1081 847.3 0 1081 127 1030 847.3 0 1030 128 724 847.3 0 724 129 910 847.3 0 910 130 1075 847.3 0 1075 131 1639 847.3 0 1639 132 2615 847.3 0 2615 133 1522 847.3 0 1522 134 1734 847.3 0 1734 135 2053 847.3 0 2053 136 1759 847.3 0 1759 137 2128 847.3 0 2128 138 2988 847.3 0 2988 139 16170 847.3 0 16170 140 27028 847.3 0 27028 141 19626 847.3 0 19626 142 8129 847.3 0 8129 143 4610 847.3 0 4610 144 6726 847.3 0 6726 145 77151 847.3 0 77151 146 70797 847.3 0 70797 147 92827 847.3 0 92827 148 31193 847.3 0 31193 149 16058 847.3 0 16058 150 2116 847.3 0 2116 151 2030 847.3 0 2030 152 1443 847.3 0 1443 153 1263 847.3 0 1263 154 1047 847.3 0 1047 155 753 847.3 0 753 156 371 847.3 0 371 157 550 847.3 0 550 158 486 847.3 0 486 159 1004 847.3 0 1004 160 654 847.3 0 654 161 355 847.3 0 355 162 465 847.3 0 465 163 626 847.3 0 626 164 1049 847.3 0 1049 165 11773 847.3 0 11773 166 9094 847.3 0 9094 167 6010 847.3 0 6010 168 331 847.3 0 331 169 409 847.3 0 409 170 179 847.3 0 179 171 179 847.3 0 179 172 176 847.3 0 176 173 238 847.3 0 238 174 250 847.3 0 250 175 221 847.3 0 221 176 175 847.3 0 175 177 150 847.3 0 150 178 143 847.3 0 143 179 138 847.3 0 138 180 144 847.3 0 144 181 174 847.3 0 174 182 169 847.3 0 169 183 201 847.3 0 201 184 172 847.3 0 172 185 143 847.3 0 143 186 152 847.3 0 152 187 189 847.3 0 189 188 152 847.3 0 152 189 160 847.3 0 160 190 182 847.3 0 182 191 205 847.3 0 205 192 312 847.3 0 312 193 315 847.3 0 315 194 267 847.3 0 267 195 187 847.3 0 187 196 160 847.3 0 160 197 172 847.3 0 172 198 148 847.3 0 148 199 265 847.3 0 265 200 288 847.3 0 288 201 309 847.3 0 309 202 159 847.3 0 159 203 136 847.3 0 136 204 143 847.3 0 143 205 180 847.3 0 180 206 170 847.3 0 170 207 269 847.3 0 269 208 157 847.3 0 157 209 205 847.3 0 205 210 177 847.3 0 177 211 159 847.3 0 159 212 152 847.3 0 152 213 158 847.3 0 158 214 139 847.3 0 139 215 169 847.3 0 169 216 495 847.3 0 495 217 760 847.3 0 760 218 1319 847.3 0 1319 219 1360 847.3 0 1360 220 181 847.3 0 181 221 182 847.3 0 182 222 139 847.3 0 139 223 166 847.3 0 166 224 135 847.3 0 135 225 142 847.3 0 142 226 159 847.3 0 159 227 148 847.3 0 148 228 190 847.3 0 190 229 133 847.3 0 133 230 131 847.3 0 131 231 128 847.3 0 128 232 123 847.3 0 123 233 123 847.3 0 123 234 115 847.3 0 115 235 123 847.3 0 123 236 113 847.3 0 113 237 132 847.3 0 132 238 145 847.3 0 145 239 119 847.3 0 119 240 148 847.3 0 148 241 128 847.3 0 128 242 139 847.3 0 139 243 167 847.3 0 167 244 126 847.3 0 126 245 126 847.3 0 126 246 197 847.3 0 197 247 169 847.3 0 169 248 141 847.3 0 141 249 133 847.3 0 133 250 111 847.3 0 111 251 99 847.3 0 99 252 143 847.3 0 143 253 120 847.3 0 120 254 91 847.3 0 91 255 137 847.3 0 137 256 160 847.3 0 160 257 130 847.3 0 130 258 118 847.3 0 118 259 134 847.3 0 134 260 151 847.3 0 151 261 156 847.3 0 156 262 286 847.3 0 286 263 222 847.3 0 222 264 161 847.3 0 161 265 195 847.3 0 195 266 178 847.3 0 178 267 165 847.3 0 165 268 123 847.3 0 123 269 102 847.3 0 102 270 100 847.3 0 100 271 140 847.3 0 140 272 130 847.3 0 130 273 121 847.3 0 121 274 132 847.3 0 132 275 153 847.3 0 153 276 136 847.3 0 136 277 151 847.3 0 151 278 174 847.3 0 174 279 145 847.3 0 145 280 124 847.3 0 124 281 156 847.3 0 156 282 132 847.3 0 132 283 121 847.3 0 121 284 128 847.3 0 128 285 138 847.3 0 138 286 124 847.3 0 124 287 135 847.3 0 135 288 186 847.3 0 186 289 160 847.3 0 160 290 145 847.3 0 145 291 169 847.3 0 169 292 144 847.3 0 144 293 145 847.3 0 145 294 157 847.3 0 157 295 158 847.3 0 158 296 166 847.3 0 166 297 242 847.3 0 242 298 221 847.3 0 221 299 244 847.3 0 244 300 394 847.3 0 394 301 2926 847.3 0 2926 Finished in 33.74 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 643,628 Reads with adapters: 24,433 (3.8%) Reads that were too short: 1,809 (0.3%) Reads written (passing filters): 22,624 (3.5%) Total basepairs processed: 109,019,575 bp Total written (filtered): 4,804,397 bp (4.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24433 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 6 0.0 3 0 0 5 1 16 131 0.0 3 0 119 12 17 12866 0.0 3 12162 583 97 24 18 130 0.0 3 17 41 7 65 19 15 0.0 3 2 0 10 3 20 62 0.0 3 6 2 0 54 22 1 0.0 3 0 0 0 1 23 71 0.0 3 0 0 1 70 24 1 0.0 3 0 0 0 1 25 5 0.0 3 0 0 0 5 26 4967 0.0 3 0 0 41 4926 27 22 0.0 3 0 0 0 22 28 7 0.0 3 0 0 0 7 29 574 0.0 3 0 0 22 552 30 5 0.0 3 0 0 0 5 31 1 0.0 3 1 32 5 0.0 3 0 0 1 4 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 9 0.0 3 0 0 0 9 43 1 0.0 3 0 0 0 1 45 8 0.0 3 0 0 0 8 46 5 0.0 3 1 0 0 4 47 16 0.0 3 0 0 0 16 48 25 0.0 3 0 0 1 24 49 9 0.0 3 0 0 1 8 50 199 0.0 3 0 0 2 197 51 6 0.0 3 0 0 1 5 52 65 0.0 3 0 0 1 64 53 161 0.0 3 0 0 2 159 54 6 0.0 3 0 0 1 5 55 2 0.0 3 0 0 0 2 56 4 0.0 3 0 0 1 3 57 2 0.0 3 0 0 0 2 59 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 13 0.0 3 0 0 0 13 71 14 0.0 3 0 0 2 12 72 1561 0.0 3 0 0 14 1547 73 282 0.0 3 0 0 1 281 74 1156 0.0 3 0 0 5 1151 75 3 0.0 3 0 0 0 3 76 29 0.0 3 0 0 1 28 77 177 0.0 3 0 0 5 172 78 8 0.0 3 0 0 0 8 79 10 0.0 3 0 0 0 10 80 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 86 32 0.0 3 0 0 0 32 87 12 0.0 3 0 0 0 12 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 14 0.0 3 0 0 0 14 91 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 11 0.0 3 0 0 1 10 98 9 0.0 3 0 0 0 9 99 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 101 5 0.0 3 0 0 0 5 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 6 0.0 3 0 0 0 6 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 23 0.0 3 0 0 0 23 111 15 0.0 3 0 0 0 15 112 1 0.0 3 0 0 0 1 113 8 0.0 3 0 0 0 8 114 10 0.0 3 0 0 1 9 115 1 0.0 3 0 0 0 1 116 4 0.0 3 0 0 0 4 117 4 0.0 3 0 0 1 3 119 5 0.0 3 0 0 0 5 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 7 0.0 3 0 0 1 6 125 13 0.0 3 0 0 0 13 126 9 0.0 3 0 0 0 9 127 38 0.0 3 0 0 2 36 128 62 0.0 3 0 0 10 52 129 32 0.0 3 0 0 0 32 131 3 0.0 3 0 0 0 3 132 6 0.0 3 0 0 0 6 133 27 0.0 3 0 0 1 26 134 8 0.0 3 0 0 0 8 135 10 0.0 3 0 0 1 9 136 2 0.0 3 0 0 0 2 137 10 0.0 3 0 0 1 9 138 490 0.0 3 0 0 4 486 139 58 0.0 3 0 0 0 58 140 83 0.0 3 0 0 6 77 141 371 0.0 3 0 0 14 357 142 20 0.0 3 0 0 1 19 143 3 0.0 3 0 0 0 3 144 2 0.0 3 0 0 0 2 145 3 0.0 3 0 0 0 3 146 29 0.0 3 0 0 1 28 147 11 0.0 3 0 0 0 11 148 4 0.0 3 0 0 1 3 149 5 0.0 3 0 0 0 5 150 5 0.0 3 0 0 0 5 151 8 0.0 3 0 0 0 8 152 3 0.0 3 0 0 0 3 153 4 0.0 3 0 0 0 4 154 4 0.0 3 0 0 0 4 155 6 0.0 3 0 0 0 6 156 85 0.0 3 0 0 2 83 157 5 0.0 3 0 0 0 5 158 6 0.0 3 0 0 0 6 159 3 0.0 3 1 0 0 2 160 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 162 4 0.0 3 0 0 0 4 163 6 0.0 3 0 0 0 6 164 5 0.0 3 0 0 0 5 165 10 0.0 3 0 0 0 10 166 30 0.0 3 0 0 1 29 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 4 0.0 3 0 0 0 4 171 5 0.0 3 0 0 0 5 172 5 0.0 3 0 0 0 5 173 2 0.0 3 0 0 0 2 174 11 0.0 3 0 0 0 11 175 6 0.0 3 0 0 0 6 176 5 0.0 3 0 0 0 5 177 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 180 3 0.0 3 0 0 0 3 181 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 211 3 0.0 3 0 0 0 3 212 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 259 2 0.0 3 0 0 0 2 263 1 0.0 3 0 0 1 270 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 22,624 Reads with adapters: 491 (2.2%) Reads written (passing filters): 22,624 (100.0%) Total basepairs processed: 4,804,397 bp Total written (filtered): 4,802,892 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 491 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.3% C: 17.5% G: 25.9% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 462 353.5 0 462 4 27 88.4 0 27 5 1 22.1 0 1 6 1 5.5 0 1