This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 164.98 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 160,373 (4.6%) Reads that were too short: 18,259 (0.5%) Reads written (passing filters): 142,114 (4.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 34,556,903 bp (3.3%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 160373 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1938 847.3 0 1938 7 39120 847.3 0 39120 8 2745 847.3 0 2745 9 46189 847.3 0 46189 10 74 847.3 0 74 11 57 847.3 0 57 12 59 847.3 0 59 13 37 847.3 0 37 14 41 847.3 0 41 15 37 847.3 0 37 16 35 847.3 0 35 17 34 847.3 0 34 18 33 847.3 0 33 19 40 847.3 0 40 20 23 847.3 0 23 21 49 847.3 0 49 22 44 847.3 0 44 23 42 847.3 0 42 24 25 847.3 0 25 25 36 847.3 0 36 26 31 847.3 0 31 27 39 847.3 0 39 28 63 847.3 0 63 29 66 847.3 0 66 30 55 847.3 0 55 31 44 847.3 0 44 32 50 847.3 0 50 33 235 847.3 0 235 34 557 847.3 0 557 35 1337 847.3 0 1337 36 25 847.3 0 25 37 26 847.3 0 26 38 48 847.3 0 48 39 45 847.3 0 45 40 28 847.3 0 28 41 45 847.3 0 45 42 29 847.3 0 29 43 43 847.3 0 43 44 54 847.3 0 54 45 59 847.3 0 59 46 48 847.3 0 48 47 69 847.3 0 69 48 36 847.3 0 36 49 40 847.3 0 40 50 56 847.3 0 56 51 44 847.3 0 44 52 23 847.3 0 23 53 42 847.3 0 42 54 51 847.3 0 51 55 45 847.3 0 45 56 83 847.3 0 83 57 466 847.3 0 466 58 405 847.3 0 405 59 84 847.3 0 84 60 54 847.3 0 54 61 33 847.3 0 33 62 45 847.3 0 45 63 34 847.3 0 34 64 56 847.3 0 56 65 48 847.3 0 48 66 45 847.3 0 45 67 49 847.3 0 49 68 68 847.3 0 68 69 49 847.3 0 49 70 45 847.3 0 45 71 36 847.3 0 36 72 84 847.3 0 84 73 520 847.3 0 520 74 244 847.3 0 244 75 52 847.3 0 52 76 53 847.3 0 53 77 36 847.3 0 36 78 58 847.3 0 58 79 83 847.3 0 83 80 37 847.3 0 37 81 40 847.3 0 40 82 45 847.3 0 45 83 42 847.3 0 42 84 109 847.3 0 109 85 327 847.3 0 327 86 477 847.3 0 477 87 266 847.3 0 266 88 42 847.3 0 42 89 56 847.3 0 56 90 105 847.3 0 105 91 82 847.3 0 82 92 54 847.3 0 54 93 49 847.3 0 49 94 93 847.3 0 93 95 61 847.3 0 61 96 60 847.3 0 60 97 70 847.3 0 70 98 67 847.3 0 67 99 70 847.3 0 70 100 42 847.3 0 42 101 52 847.3 0 52 102 49 847.3 0 49 103 54 847.3 0 54 104 72 847.3 0 72 105 79 847.3 0 79 106 115 847.3 0 115 107 2024 847.3 0 2024 108 1153 847.3 0 1153 109 829 847.3 0 829 110 80 847.3 0 80 111 37 847.3 0 37 112 53 847.3 0 53 113 54 847.3 0 54 114 60 847.3 0 60 115 42 847.3 0 42 116 64 847.3 0 64 117 53 847.3 0 53 118 34 847.3 0 34 119 47 847.3 0 47 120 54 847.3 0 54 121 78 847.3 0 78 122 61 847.3 0 61 123 106 847.3 0 106 124 60 847.3 0 60 125 73 847.3 0 73 126 118 847.3 0 118 127 1159 847.3 0 1159 128 868 847.3 0 868 129 343 847.3 0 343 130 154 847.3 0 154 131 68 847.3 0 68 132 59 847.3 0 59 133 64 847.3 0 64 134 69 847.3 0 69 135 92 847.3 0 92 136 178 847.3 0 178 137 159 847.3 0 159 138 134 847.3 0 134 139 79 847.3 0 79 140 177 847.3 0 177 141 328 847.3 0 328 142 421 847.3 0 421 143 2909 847.3 0 2909 144 1572 847.3 0 1572 145 520 847.3 0 520 146 127 847.3 0 127 147 460 847.3 0 460 148 92 847.3 0 92 149 171 847.3 0 171 150 109 847.3 0 109 151 114 847.3 0 114 152 152 847.3 0 152 153 317 847.3 0 317 154 592 847.3 0 592 155 1729 847.3 0 1729 156 1109 847.3 0 1109 157 82 847.3 0 82 158 115 847.3 0 115 159 114 847.3 0 114 160 90 847.3 0 90 161 90 847.3 0 90 162 112 847.3 0 112 163 43 847.3 0 43 164 56 847.3 0 56 165 60 847.3 0 60 166 110 847.3 0 110 167 122 847.3 0 122 168 87 847.3 0 87 169 296 847.3 0 296 170 195 847.3 0 195 171 153 847.3 0 153 172 63 847.3 0 63 173 101 847.3 0 101 174 187 847.3 0 187 175 211 847.3 0 211 176 208 847.3 0 208 177 84 847.3 0 84 178 90 847.3 0 90 179 107 847.3 0 107 180 130 847.3 0 130 181 156 847.3 0 156 182 327 847.3 0 327 183 265 847.3 0 265 184 390 847.3 0 390 185 415 847.3 0 415 186 3314 847.3 0 3314 187 4131 847.3 0 4131 188 10571 847.3 0 10571 189 125 847.3 0 125 190 55 847.3 0 55 191 65 847.3 0 65 192 60 847.3 0 60 193 46 847.3 0 46 194 76 847.3 0 76 195 118 847.3 0 118 196 60 847.3 0 60 197 50 847.3 0 50 198 48 847.3 0 48 199 61 847.3 0 61 200 77 847.3 0 77 201 57 847.3 0 57 202 56 847.3 0 56 203 88 847.3 0 88 204 186 847.3 0 186 205 113 847.3 0 113 206 80 847.3 0 80 207 67 847.3 0 67 208 90 847.3 0 90 209 121 847.3 0 121 210 114 847.3 0 114 211 79 847.3 0 79 212 66 847.3 0 66 213 89 847.3 0 89 214 70 847.3 0 70 215 96 847.3 0 96 216 84 847.3 0 84 217 334 847.3 0 334 218 894 847.3 0 894 219 618 847.3 0 618 220 79 847.3 0 79 221 66 847.3 0 66 222 93 847.3 0 93 223 82 847.3 0 82 224 52 847.3 0 52 225 70 847.3 0 70 226 69 847.3 0 69 227 101 847.3 0 101 228 122 847.3 0 122 229 50 847.3 0 50 230 83 847.3 0 83 231 101 847.3 0 101 232 98 847.3 0 98 233 85 847.3 0 85 234 77 847.3 0 77 235 101 847.3 0 101 236 91 847.3 0 91 237 99 847.3 0 99 238 66 847.3 0 66 239 83 847.3 0 83 240 95 847.3 0 95 241 133 847.3 0 133 242 156 847.3 0 156 243 211 847.3 0 211 244 137 847.3 0 137 245 176 847.3 0 176 246 211 847.3 0 211 247 128 847.3 0 128 248 111 847.3 0 111 249 79 847.3 0 79 250 445 847.3 0 445 251 400 847.3 0 400 252 198 847.3 0 198 253 169 847.3 0 169 254 239 847.3 0 239 255 271 847.3 0 271 256 88 847.3 0 88 257 99 847.3 0 99 258 106 847.3 0 106 259 102 847.3 0 102 260 92 847.3 0 92 261 170 847.3 0 170 262 276 847.3 0 276 263 213 847.3 0 213 264 154 847.3 0 154 265 65 847.3 0 65 266 92 847.3 0 92 267 92 847.3 0 92 268 82 847.3 0 82 269 119 847.3 0 119 270 154 847.3 0 154 271 219 847.3 0 219 272 191 847.3 0 191 273 196 847.3 0 196 274 81 847.3 0 81 275 90 847.3 0 90 276 120 847.3 0 120 277 115 847.3 0 115 278 277 847.3 0 277 279 296 847.3 0 296 280 701 847.3 0 701 281 499 847.3 0 499 282 552 847.3 0 552 283 150 847.3 0 150 284 118 847.3 0 118 285 194 847.3 0 194 286 199 847.3 0 199 287 97 847.3 0 97 288 108 847.3 0 108 289 172 847.3 0 172 290 190 847.3 0 190 291 184 847.3 0 184 292 118 847.3 0 118 293 128 847.3 0 128 294 161 847.3 0 161 295 240 847.3 0 240 296 374 847.3 0 374 297 649 847.3 0 649 298 709 847.3 0 709 299 668 847.3 0 668 300 373 847.3 0 373 301 114 847.3 0 114 Finished in 9.85 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 142,114 Reads with adapters: 50,193 (35.3%) Reads that were too short: 140 (0.1%) Reads written (passing filters): 50,053 (35.2%) Total basepairs processed: 34,556,903 bp Total written (filtered): 13,705,961 bp (39.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50193 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 36 0.0 3 0 0 16 20 16 466 0.0 3 0 411 41 14 17 48793 0.0 3 45977 2342 385 89 18 111 0.0 3 7 89 6 9 19 65 0.0 3 2 0 40 23 20 168 0.0 3 0 0 0 168 23 2 0.0 3 1 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 31 17 0.0 3 0 0 1 16 32 2 0.0 3 1 0 0 1 33 1 0.0 3 0 0 0 1 34 4 0.0 3 0 0 0 4 37 21 0.0 3 0 0 0 21 40 3 0.0 3 0 0 0 3 41 2 0.0 3 0 0 0 2 43 10 0.0 3 0 0 0 10 47 1 0.0 3 0 0 0 1 48 102 0.0 3 0 0 0 102 50 1 0.0 3 0 0 0 1 51 8 0.0 3 0 0 0 8 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 56 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 59 47 0.0 3 0 0 0 47 60 9 0.0 3 0 0 0 9 61 31 0.0 3 0 0 0 31 62 8 0.0 3 0 0 1 7 63 4 0.0 3 0 0 1 3 64 7 0.0 3 0 0 0 7 65 9 0.0 3 0 0 0 9 66 5 0.0 3 0 0 0 5 67 3 0.0 3 0 0 0 3 68 4 0.0 3 0 0 0 4 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 84 19 0.0 3 0 0 0 19 85 7 0.0 3 0 0 0 7 87 2 0.0 3 0 0 1 1 89 2 0.0 3 0 0 1 1 90 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 3 0.0 3 0 0 1 2 110 2 0.0 3 0 0 1 1 111 6 0.0 3 0 0 0 6 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 132 58 0.0 3 0 0 0 58 134 19 0.0 3 0 0 0 19 135 4 0.0 3 0 0 0 4 136 2 0.0 3 0 0 1 1 137 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 1 0 2 149 1 0.0 3 0 0 0 1 150 11 0.0 3 0 0 0 11 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 177 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 1 1 192 2 0.0 3 0 0 0 2 194 5 0.0 3 0 0 0 5 196 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.97 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 50,053 Reads with adapters: 2,170 (4.3%) Reads written (passing filters): 50,053 (100.0%) Total basepairs processed: 13,705,961 bp Total written (filtered): 13,699,396 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2170 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.0% C: 11.8% G: 16.6% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2117 782.1 0 2117 4 51 195.5 0 51 5 2 48.9 0 2