This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 164.75 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 96,203 (2.8%) Reads that were too short: 11,059 (0.3%) Reads written (passing filters): 85,144 (2.5%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 21,179,088 bp (2.0%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 96203 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 17810 847.3 0 17810 7 928 847.3 0 928 8 28774 847.3 0 28774 9 3262 847.3 0 3262 10 17 847.3 0 17 11 11 847.3 0 11 12 15 847.3 0 15 13 18 847.3 0 18 14 16 847.3 0 16 15 15 847.3 0 15 16 14 847.3 0 14 17 33 847.3 0 33 18 22 847.3 0 22 19 17 847.3 0 17 20 15 847.3 0 15 21 18 847.3 0 18 22 33 847.3 0 33 23 15 847.3 0 15 24 21 847.3 0 21 25 18 847.3 0 18 26 19 847.3 0 19 27 21 847.3 0 21 28 22 847.3 0 22 29 13 847.3 0 13 30 27 847.3 0 27 31 21 847.3 0 21 32 14 847.3 0 14 33 28 847.3 0 28 34 35 847.3 0 35 35 16 847.3 0 16 36 23 847.3 0 23 37 19 847.3 0 19 38 16 847.3 0 16 39 15 847.3 0 15 40 31 847.3 0 31 41 17 847.3 0 17 42 23 847.3 0 23 43 23 847.3 0 23 44 26 847.3 0 26 45 30 847.3 0 30 46 40 847.3 0 40 47 85 847.3 0 85 48 35 847.3 0 35 49 215 847.3 0 215 50 256 847.3 0 256 51 22 847.3 0 22 52 22 847.3 0 22 53 35 847.3 0 35 54 13 847.3 0 13 55 29 847.3 0 29 56 20 847.3 0 20 57 24 847.3 0 24 58 44 847.3 0 44 59 60 847.3 0 60 60 59 847.3 0 59 61 44 847.3 0 44 62 49 847.3 0 49 63 19 847.3 0 19 64 24 847.3 0 24 65 16 847.3 0 16 66 301 847.3 0 301 67 277 847.3 0 277 68 160 847.3 0 160 69 13 847.3 0 13 70 21 847.3 0 21 71 14 847.3 0 14 72 12 847.3 0 12 73 30 847.3 0 30 74 20 847.3 0 20 75 19 847.3 0 19 76 27 847.3 0 27 77 18 847.3 0 18 78 18 847.3 0 18 79 20 847.3 0 20 80 28 847.3 0 28 81 18 847.3 0 18 82 65 847.3 0 65 83 63 847.3 0 63 84 53 847.3 0 53 85 38 847.3 0 38 86 55 847.3 0 55 87 24 847.3 0 24 88 22 847.3 0 22 89 211 847.3 0 211 90 70 847.3 0 70 91 57 847.3 0 57 92 65 847.3 0 65 93 118 847.3 0 118 94 66 847.3 0 66 95 45 847.3 0 45 96 27 847.3 0 27 97 54 847.3 0 54 98 87 847.3 0 87 99 76 847.3 0 76 100 60 847.3 0 60 101 60 847.3 0 60 102 59 847.3 0 59 103 122 847.3 0 122 104 167 847.3 0 167 105 62 847.3 0 62 106 78 847.3 0 78 107 111 847.3 0 111 108 94 847.3 0 94 109 84 847.3 0 84 110 145 847.3 0 145 111 305 847.3 0 305 112 356 847.3 0 356 113 326 847.3 0 326 114 196 847.3 0 196 115 398 847.3 0 398 116 724 847.3 0 724 117 9076 847.3 0 9076 118 6607 847.3 0 6607 119 4403 847.3 0 4403 120 56 847.3 0 56 121 47 847.3 0 47 122 32 847.3 0 32 123 21 847.3 0 21 124 26 847.3 0 26 125 37 847.3 0 37 126 126 847.3 0 126 127 90 847.3 0 90 128 34 847.3 0 34 129 44 847.3 0 44 130 40 847.3 0 40 131 77 847.3 0 77 132 86 847.3 0 86 133 70 847.3 0 70 134 67 847.3 0 67 135 37 847.3 0 37 136 53 847.3 0 53 137 51 847.3 0 51 138 82 847.3 0 82 139 45 847.3 0 45 140 56 847.3 0 56 141 122 847.3 0 122 142 66 847.3 0 66 143 32 847.3 0 32 144 63 847.3 0 63 145 79 847.3 0 79 146 109 847.3 0 109 147 109 847.3 0 109 148 69 847.3 0 69 149 62 847.3 0 62 150 82 847.3 0 82 151 951 847.3 0 951 152 841 847.3 0 841 153 501 847.3 0 501 154 141 847.3 0 141 155 43 847.3 0 43 156 40 847.3 0 40 157 46 847.3 0 46 158 52 847.3 0 52 159 34 847.3 0 34 160 39 847.3 0 39 161 47 847.3 0 47 162 156 847.3 0 156 163 86 847.3 0 86 164 54 847.3 0 54 165 52 847.3 0 52 166 44 847.3 0 44 167 36 847.3 0 36 168 33 847.3 0 33 169 61 847.3 0 61 170 40 847.3 0 40 171 47 847.3 0 47 172 54 847.3 0 54 173 51 847.3 0 51 174 26 847.3 0 26 175 23 847.3 0 23 176 34 847.3 0 34 177 29 847.3 0 29 178 36 847.3 0 36 179 92 847.3 0 92 180 68 847.3 0 68 181 124 847.3 0 124 182 150 847.3 0 150 183 55 847.3 0 55 184 48 847.3 0 48 185 118 847.3 0 118 186 33 847.3 0 33 187 35 847.3 0 35 188 35 847.3 0 35 189 39 847.3 0 39 190 51 847.3 0 51 191 47 847.3 0 47 192 109 847.3 0 109 193 33 847.3 0 33 194 26 847.3 0 26 195 33 847.3 0 33 196 31 847.3 0 31 197 34 847.3 0 34 198 45 847.3 0 45 199 44 847.3 0 44 200 41 847.3 0 41 201 39 847.3 0 39 202 33 847.3 0 33 203 29 847.3 0 29 204 48 847.3 0 48 205 40 847.3 0 40 206 50 847.3 0 50 207 51 847.3 0 51 208 40 847.3 0 40 209 38 847.3 0 38 210 40 847.3 0 40 211 51 847.3 0 51 212 41 847.3 0 41 213 76 847.3 0 76 214 67 847.3 0 67 215 52 847.3 0 52 216 77 847.3 0 77 217 112 847.3 0 112 218 312 847.3 0 312 219 345 847.3 0 345 220 411 847.3 0 411 221 203 847.3 0 203 222 35 847.3 0 35 223 44 847.3 0 44 224 60 847.3 0 60 225 72 847.3 0 72 226 108 847.3 0 108 227 82 847.3 0 82 228 77 847.3 0 77 229 127 847.3 0 127 230 74 847.3 0 74 231 256 847.3 0 256 232 310 847.3 0 310 233 609 847.3 0 609 234 322 847.3 0 322 235 137 847.3 0 137 236 111 847.3 0 111 237 115 847.3 0 115 238 99 847.3 0 99 239 81 847.3 0 81 240 49 847.3 0 49 241 36 847.3 0 36 242 57 847.3 0 57 243 71 847.3 0 71 244 81 847.3 0 81 245 148 847.3 0 148 246 197 847.3 0 197 247 272 847.3 0 272 248 181 847.3 0 181 249 97 847.3 0 97 250 88 847.3 0 88 251 121 847.3 0 121 252 185 847.3 0 185 253 109 847.3 0 109 254 225 847.3 0 225 255 195 847.3 0 195 256 129 847.3 0 129 257 52 847.3 0 52 258 58 847.3 0 58 259 75 847.3 0 75 260 43 847.3 0 43 261 50 847.3 0 50 262 48 847.3 0 48 263 60 847.3 0 60 264 64 847.3 0 64 265 80 847.3 0 80 266 90 847.3 0 90 267 84 847.3 0 84 268 78 847.3 0 78 269 56 847.3 0 56 270 44 847.3 0 44 271 94 847.3 0 94 272 110 847.3 0 110 273 67 847.3 0 67 274 70 847.3 0 70 275 49 847.3 0 49 276 50 847.3 0 50 277 59 847.3 0 59 278 203 847.3 0 203 279 172 847.3 0 172 280 136 847.3 0 136 281 70 847.3 0 70 282 93 847.3 0 93 283 148 847.3 0 148 284 130 847.3 0 130 285 83 847.3 0 83 286 112 847.3 0 112 287 90 847.3 0 90 288 91 847.3 0 91 289 58 847.3 0 58 290 73 847.3 0 73 291 121 847.3 0 121 292 126 847.3 0 126 293 179 847.3 0 179 294 90 847.3 0 90 295 90 847.3 0 90 296 73 847.3 0 73 297 76 847.3 0 76 298 91 847.3 0 91 299 115 847.3 0 115 300 115 847.3 0 115 301 67 847.3 0 67 Finished in 5.95 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 85,144 Reads with adapters: 30,431 (35.7%) Reads that were too short: 197 (0.2%) Reads written (passing filters): 30,234 (35.5%) Total basepairs processed: 21,179,088 bp Total written (filtered): 8,248,165 bp (38.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30431 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 6 3 16 71 0.0 3 0 51 13 7 17 4062 0.0 3 3575 419 59 9 18 25407 0.0 3 23985 1170 191 61 19 100 0.0 3 10 57 16 17 20 37 0.0 3 0 0 20 17 21 62 0.0 3 0 0 0 62 23 1 0.0 3 0 1 24 1 0.0 3 0 0 0 1 27 4 0.0 3 0 0 0 4 30 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 40 47 0.0 3 1 0 0 46 43 9 0.0 3 0 0 0 9 50 5 0.0 3 0 0 0 5 53 3 0.0 3 0 0 0 3 54 278 0.0 3 0 0 4 274 56 14 0.0 3 0 0 0 14 57 38 0.0 3 0 0 0 38 58 15 0.0 3 0 0 0 15 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 0 4 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 5 0.0 3 0 0 0 5 70 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 1 78 2 0.0 3 0 0 0 2 80 10 0.0 3 0 0 0 10 82 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 91 4 0.0 3 0 0 0 4 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 8 0.0 3 0 0 0 8 106 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 1 165 1 0.0 3 0 0 0 1 166 40 0.0 3 0 0 0 40 167 23 0.0 3 0 0 0 23 168 4 0.0 3 0 0 0 4 169 58 0.0 3 0 0 5 53 170 6 0.0 3 0 0 1 5 171 3 0.0 3 0 0 0 3 172 1 0.0 3 0 0 0 1 173 5 0.0 3 0 0 0 5 174 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 1 2 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.76 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 30,234 Reads with adapters: 2,671 (8.8%) Reads written (passing filters): 30,234 (100.0%) Total basepairs processed: 8,248,165 bp Total written (filtered): 8,240,131 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2671 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.5% C: 9.5% G: 10.9% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2652 472.4 0 2652 4 17 118.1 0 17 5 2 29.5 0 2