This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.90 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 648,457 (18.7%) Reads that were too short: 45,448 (1.3%) Reads written (passing filters): 603,009 (17.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 148,436,461 bp (14.2%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 648457 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 521 847.3 0 521 7 5271 847.3 0 5271 8 4069 847.3 0 4069 9 388 847.3 0 388 10 444 847.3 0 444 11 301 847.3 0 301 12 301 847.3 0 301 13 796 847.3 0 796 14 719 847.3 0 719 15 710 847.3 0 710 16 599 847.3 0 599 17 930 847.3 0 930 18 1267 847.3 0 1267 19 2067 847.3 0 2067 20 43713 847.3 0 43713 21 37487 847.3 0 37487 22 27681 847.3 0 27681 23 375 847.3 0 375 24 247 847.3 0 247 25 417 847.3 0 417 26 208 847.3 0 208 27 186 847.3 0 186 28 157 847.3 0 157 29 347 847.3 0 347 30 184 847.3 0 184 31 340 847.3 0 340 32 578 847.3 0 578 33 961 847.3 0 961 34 956 847.3 0 956 35 1757 847.3 0 1757 36 3076 847.3 0 3076 37 880 847.3 0 880 38 1103 847.3 0 1103 39 1876 847.3 0 1876 40 1215 847.3 0 1215 41 1050 847.3 0 1050 42 1901 847.3 0 1901 43 1547 847.3 0 1547 44 2089 847.3 0 2089 45 1910 847.3 0 1910 46 2890 847.3 0 2890 47 4400 847.3 0 4400 48 8728 847.3 0 8728 49 163878 847.3 0 163878 50 141551 847.3 0 141551 51 72291 847.3 0 72291 52 347 847.3 0 347 53 73 847.3 0 73 54 110 847.3 0 110 55 34 847.3 0 34 56 103 847.3 0 103 57 90 847.3 0 90 58 101 847.3 0 101 59 57 847.3 0 57 60 124 847.3 0 124 61 89 847.3 0 89 62 149 847.3 0 149 63 1237 847.3 0 1237 64 1119 847.3 0 1119 65 933 847.3 0 933 66 42 847.3 0 42 67 35 847.3 0 35 68 52 847.3 0 52 69 48 847.3 0 48 70 52 847.3 0 52 71 53 847.3 0 53 72 58 847.3 0 58 73 46 847.3 0 46 74 45 847.3 0 45 75 55 847.3 0 55 76 69 847.3 0 69 77 43 847.3 0 43 78 38 847.3 0 38 79 51 847.3 0 51 80 38 847.3 0 38 81 60 847.3 0 60 82 46 847.3 0 46 83 55 847.3 0 55 84 54 847.3 0 54 85 50 847.3 0 50 86 59 847.3 0 59 87 44 847.3 0 44 88 235 847.3 0 235 89 75 847.3 0 75 90 79 847.3 0 79 91 127 847.3 0 127 92 68 847.3 0 68 93 52 847.3 0 52 94 61 847.3 0 61 95 57 847.3 0 57 96 37 847.3 0 37 97 33 847.3 0 33 98 60 847.3 0 60 99 42 847.3 0 42 100 29 847.3 0 29 101 31 847.3 0 31 102 47 847.3 0 47 103 24 847.3 0 24 104 34 847.3 0 34 105 35 847.3 0 35 106 73 847.3 0 73 107 42 847.3 0 42 108 45 847.3 0 45 109 33 847.3 0 33 110 60 847.3 0 60 111 60 847.3 0 60 112 64 847.3 0 64 113 55 847.3 0 55 114 51 847.3 0 51 115 50 847.3 0 50 116 55 847.3 0 55 117 177 847.3 0 177 118 326 847.3 0 326 119 814 847.3 0 814 120 71 847.3 0 71 121 52 847.3 0 52 122 51 847.3 0 51 123 49 847.3 0 49 124 85 847.3 0 85 125 118 847.3 0 118 126 67 847.3 0 67 127 63 847.3 0 63 128 49 847.3 0 49 129 75 847.3 0 75 130 47 847.3 0 47 131 37 847.3 0 37 132 44 847.3 0 44 133 55 847.3 0 55 134 73 847.3 0 73 135 72 847.3 0 72 136 50 847.3 0 50 137 54 847.3 0 54 138 63 847.3 0 63 139 57 847.3 0 57 140 77 847.3 0 77 141 66 847.3 0 66 142 120 847.3 0 120 143 104 847.3 0 104 144 128 847.3 0 128 145 132 847.3 0 132 146 99 847.3 0 99 147 63 847.3 0 63 148 37 847.3 0 37 149 87 847.3 0 87 150 95 847.3 0 95 151 96 847.3 0 96 152 94 847.3 0 94 153 93 847.3 0 93 154 59 847.3 0 59 155 78 847.3 0 78 156 69 847.3 0 69 157 93 847.3 0 93 158 111 847.3 0 111 159 124 847.3 0 124 160 74 847.3 0 74 161 66 847.3 0 66 162 45 847.3 0 45 163 39 847.3 0 39 164 51 847.3 0 51 165 60 847.3 0 60 166 51 847.3 0 51 167 87 847.3 0 87 168 79 847.3 0 79 169 103 847.3 0 103 170 84 847.3 0 84 171 100 847.3 0 100 172 87 847.3 0 87 173 113 847.3 0 113 174 82 847.3 0 82 175 116 847.3 0 116 176 184 847.3 0 184 177 248 847.3 0 248 178 281 847.3 0 281 179 379 847.3 0 379 180 694 847.3 0 694 181 241 847.3 0 241 182 370 847.3 0 370 183 951 847.3 0 951 184 517 847.3 0 517 185 159 847.3 0 159 186 257 847.3 0 257 187 218 847.3 0 218 188 779 847.3 0 779 189 379 847.3 0 379 190 503 847.3 0 503 191 418 847.3 0 418 192 506 847.3 0 506 193 3272 847.3 0 3272 194 5011 847.3 0 5011 195 28847 847.3 0 28847 196 820 847.3 0 820 197 324 847.3 0 324 198 67 847.3 0 67 199 53 847.3 0 53 200 105 847.3 0 105 201 80 847.3 0 80 202 71 847.3 0 71 203 68 847.3 0 68 204 64 847.3 0 64 205 83 847.3 0 83 206 82 847.3 0 82 207 73 847.3 0 73 208 130 847.3 0 130 209 97 847.3 0 97 210 72 847.3 0 72 211 72 847.3 0 72 212 86 847.3 0 86 213 419 847.3 0 419 214 1052 847.3 0 1052 215 703 847.3 0 703 216 366 847.3 0 366 217 186 847.3 0 186 218 263 847.3 0 263 219 912 847.3 0 912 220 750 847.3 0 750 221 550 847.3 0 550 222 215 847.3 0 215 223 121 847.3 0 121 224 78 847.3 0 78 225 146 847.3 0 146 226 156 847.3 0 156 227 111 847.3 0 111 228 100 847.3 0 100 229 66 847.3 0 66 230 92 847.3 0 92 231 171 847.3 0 171 232 154 847.3 0 154 233 117 847.3 0 117 234 165 847.3 0 165 235 163 847.3 0 163 236 179 847.3 0 179 237 267 847.3 0 267 238 272 847.3 0 272 239 1124 847.3 0 1124 240 1648 847.3 0 1648 241 679 847.3 0 679 242 224 847.3 0 224 243 55 847.3 0 55 244 88 847.3 0 88 245 82 847.3 0 82 246 140 847.3 0 140 247 117 847.3 0 117 248 126 847.3 0 126 249 199 847.3 0 199 250 1119 847.3 0 1119 251 1245 847.3 0 1245 252 973 847.3 0 973 253 563 847.3 0 563 254 89 847.3 0 89 255 92 847.3 0 92 256 160 847.3 0 160 257 210 847.3 0 210 258 213 847.3 0 213 259 799 847.3 0 799 260 652 847.3 0 652 261 258 847.3 0 258 262 177 847.3 0 177 263 1347 847.3 0 1347 264 1065 847.3 0 1065 265 806 847.3 0 806 266 537 847.3 0 537 267 295 847.3 0 295 268 363 847.3 0 363 269 253 847.3 0 253 270 169 847.3 0 169 271 93 847.3 0 93 272 93 847.3 0 93 273 91 847.3 0 91 274 122 847.3 0 122 275 208 847.3 0 208 276 130 847.3 0 130 277 128 847.3 0 128 278 146 847.3 0 146 279 118 847.3 0 118 280 119 847.3 0 119 281 178 847.3 0 178 282 224 847.3 0 224 283 284 847.3 0 284 284 261 847.3 0 261 285 229 847.3 0 229 286 325 847.3 0 325 287 511 847.3 0 511 288 6725 847.3 0 6725 289 5357 847.3 0 5357 290 2701 847.3 0 2701 291 349 847.3 0 349 292 139 847.3 0 139 293 195 847.3 0 195 294 260 847.3 0 260 295 289 847.3 0 289 296 236 847.3 0 236 297 320 847.3 0 320 298 314 847.3 0 314 299 300 847.3 0 300 300 245 847.3 0 245 301 219 847.3 0 219 Finished in 38.42 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 603,009 Reads with adapters: 19,106 (3.2%) Reads that were too short: 2,965 (0.5%) Reads written (passing filters): 16,141 (2.7%) Total basepairs processed: 148,436,461 bp Total written (filtered): 2,836,233 bp (1.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19106 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 2 1 16 36 0.0 3 0 34 1 1 17 4460 0.0 3 4181 240 30 9 18 19 0.0 3 2 13 0 4 19 15 0.0 3 5 3 5 2 20 6 0.0 3 0 0 0 6 21 1 0.0 3 1 24 2 0.0 3 1 0 0 1 25 2 0.0 3 1 0 0 1 27 1 0.0 3 0 0 0 1 28 14 0.0 3 0 0 0 14 29 12 0.0 3 1 0 0 11 30 1 0.0 3 0 0 0 1 31 6 0.0 3 0 0 0 6 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 4 0.0 3 0 0 0 4 35 15 0.0 3 0 0 1 14 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 46 4 0.0 3 0 0 0 4 47 3 0.0 3 0 0 0 3 49 3 0.0 3 0 0 0 3 51 17 0.0 3 0 0 0 17 52 6 0.0 3 0 0 0 6 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 1 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 0 3 72 12 0.0 3 0 0 0 12 73 2039 0.0 3 0 0 8 2031 74 119 0.0 3 0 0 1 118 75 13 0.0 3 0 0 0 13 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 10 0.0 3 0 0 0 10 81 2 0.0 3 0 0 0 2 87 41 0.0 3 0 0 0 41 88 3 0.0 3 0 0 0 3 89 4 0.0 3 0 0 0 4 90 59 0.0 3 0 0 0 59 91 5 0.0 3 0 0 0 5 92 58 0.0 3 0 0 0 58 93 18 0.0 3 0 0 5 13 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 1 96 1 0.0 3 0 0 0 1 97 48 0.0 3 0 0 0 48 98 6 0.0 3 0 0 0 6 100 6 0.0 3 0 0 0 6 101 7 0.0 3 0 0 0 7 102 192 0.0 3 0 0 3 189 103 21 0.0 3 0 0 2 19 104 11 0.0 3 0 0 0 11 105 4 0.0 3 0 0 1 3 107 1 0.0 3 0 0 1 108 5 0.0 3 0 0 0 5 110 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 5 0.0 3 0 0 0 5 117 38 0.0 3 0 0 0 38 118 30 0.0 3 0 0 0 30 119 9 0.0 3 0 0 0 9 120 11 0.0 3 0 0 0 11 121 3 0.0 3 0 0 0 3 122 1472 0.0 3 0 0 15 1457 123 1768 0.0 3 0 0 10 1758 124 1565 0.0 3 0 0 11 1554 125 318 0.0 3 0 0 7 311 126 309 0.0 3 0 0 11 298 127 187 0.0 3 0 0 6 181 128 250 0.0 3 0 0 2 248 129 10 0.0 3 0 0 1 9 130 45 0.0 3 0 0 2 43 131 47 0.0 3 0 0 2 45 132 9 0.0 3 0 0 0 9 133 7 0.0 3 0 0 0 7 134 16 0.0 3 0 0 0 16 135 3 0.0 3 0 0 0 3 136 6 0.0 3 0 0 1 5 137 66 0.0 3 0 0 0 66 138 45 0.0 3 0 0 0 45 139 44 0.0 3 0 0 0 44 140 27 0.0 3 0 0 2 25 141 15 0.0 3 0 0 1 14 142 729 0.0 3 0 0 7 722 143 29 0.0 3 0 0 0 29 144 5 0.0 3 0 0 0 5 145 87 0.0 3 0 0 1 86 146 52 0.0 3 0 0 1 51 147 13 0.0 3 0 0 2 11 148 48 0.0 3 0 0 1 47 149 56 0.0 3 0 0 1 55 150 35 0.0 3 0 0 0 35 151 360 0.0 3 0 0 3 357 152 150 0.0 3 0 0 0 150 153 107 0.0 3 0 0 1 106 154 51 0.0 3 0 0 2 49 155 16 0.0 3 0 0 2 14 156 17 0.0 3 0 0 2 15 157 11 0.0 3 0 0 0 11 158 1 0.0 3 0 0 0 1 159 4 0.0 3 0 0 0 4 160 6 0.0 3 1 0 0 5 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 8 0.0 3 0 0 0 8 164 5 0.0 3 0 0 0 5 165 7 0.0 3 0 0 0 7 166 15 0.0 3 0 0 0 15 167 10 0.0 3 0 0 1 9 168 25 0.0 3 0 0 0 25 169 10 0.0 3 0 0 1 9 170 119 0.0 3 0 0 1 118 171 529 0.0 3 0 0 5 524 172 137 0.0 3 0 0 0 137 173 21 0.0 3 0 0 0 21 174 111 0.0 3 0 0 6 105 175 66 0.0 3 1 0 3 62 176 10 0.0 3 0 0 1 9 177 21 0.0 3 0 0 0 21 178 8 0.0 3 0 0 0 8 179 7 0.0 3 0 0 0 7 180 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 21 0.0 3 0 0 1 20 184 9 0.0 3 0 0 0 9 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 3 0.0 3 0 0 0 3 190 5 0.0 3 0 0 1 4 192 2 0.0 3 0 0 0 2 193 23 0.0 3 0 0 0 23 194 3 0.0 3 0 0 0 3 195 3 0.0 3 0 0 0 3 196 3 0.0 3 0 0 1 2 197 7 0.0 3 0 0 0 7 198 3 0.0 3 0 0 0 3 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 20 0.0 3 0 0 1 19 203 4 0.0 3 0 0 0 4 204 6 0.0 3 0 0 0 6 205 11 0.0 3 0 0 0 11 206 8 0.0 3 0 0 0 8 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 3 0.0 3 0 0 0 3 213 2 0.0 3 0 0 0 2 214 3 0.0 3 0 0 0 3 215 8 0.0 3 0 0 0 8 216 3 0.0 3 0 0 0 3 217 10 0.0 3 0 0 0 10 218 6 0.0 3 0 0 0 6 219 4 0.0 3 0 0 0 4 220 5 0.0 3 0 0 0 5 221 4 0.0 3 0 0 0 4 222 8 0.0 3 0 0 1 7 223 12 0.0 3 0 0 0 12 224 4 0.0 3 0 0 0 4 225 5 0.0 3 0 0 2 3 226 4 0.0 3 0 0 0 4 229 8 0.0 3 0 0 0 8 230 2 0.0 3 0 0 0 2 231 10 0.0 3 0 0 0 10 232 1 0.0 3 0 0 0 1 233 10 0.0 3 0 0 0 10 234 187 0.0 3 0 0 2 185 235 341 0.0 3 0 0 8 333 236 338 0.0 3 0 0 5 333 237 208 0.0 3 0 0 5 203 238 383 0.0 3 0 0 13 370 239 211 0.0 3 0 0 6 205 240 70 0.0 3 0 0 2 68 241 20 0.0 3 0 0 0 20 242 24 0.0 3 0 0 0 24 243 42 0.0 3 0 0 1 41 244 82 0.0 3 0 0 5 77 245 15 0.0 3 0 0 4 11 246 5 0.0 3 0 0 0 5 247 5 0.0 3 0 0 0 5 248 7 0.0 3 0 0 1 6 249 6 0.0 3 0 0 1 5 250 28 0.0 3 0 0 1 27 251 6 0.0 3 0 0 0 6 252 38 0.0 3 0 0 0 38 253 9 0.0 3 0 0 0 9 254 4 0.0 3 0 0 1 3 255 3 0.0 3 0 0 0 3 258 1 0.0 3 0 0 0 1 260 2 0.0 3 0 0 0 2 262 4 0.0 3 0 0 0 4 263 31 0.0 3 0 0 0 31 264 38 0.0 3 0 0 2 36 265 36 0.0 3 0 0 3 33 266 20 0.0 3 0 0 1 19 267 37 0.0 3 0 0 3 34 268 14 0.0 3 0 0 3 11 269 5 0.0 3 0 0 0 5 270 4 0.0 3 0 0 0 4 271 5 0.0 3 0 0 0 5 272 30 0.0 3 0 0 1 29 273 11 0.0 3 0 0 2 9 274 1 0.0 3 0 0 0 1 275 2 0.0 3 0 0 1 1 276 2 0.0 3 0 0 0 2 277 3 0.0 3 0 0 0 3 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 6 0.0 3 0 0 0 6 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 16,141 Reads with adapters: 809 (5.0%) Reads written (passing filters): 16,141 (100.0%) Total basepairs processed: 2,836,233 bp Total written (filtered): 2,833,766 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 809 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.7% C: 17.3% G: 10.5% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 778 252.2 0 778 4 23 63.1 0 23 5 7 15.8 0 7 6 1 3.9 0 1