This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 166.95 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 321,245 (9.3%) Reads that were too short: 16,300 (0.5%) Reads written (passing filters): 304,945 (8.8%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 63,153,009 bp (6.0%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 321245 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 82 847.3 0 82 7 111 847.3 0 111 8 92 847.3 0 92 9 138 847.3 0 138 10 68 847.3 0 68 11 68 847.3 0 68 12 118 847.3 0 118 13 128 847.3 0 128 14 61 847.3 0 61 15 82 847.3 0 82 16 81 847.3 0 81 17 91 847.3 0 91 18 94 847.3 0 94 19 74 847.3 0 74 20 92 847.3 0 92 21 103 847.3 0 103 22 84 847.3 0 84 23 79 847.3 0 79 24 92 847.3 0 92 25 79 847.3 0 79 26 64 847.3 0 64 27 75 847.3 0 75 28 77 847.3 0 77 29 61 847.3 0 61 30 73 847.3 0 73 31 72 847.3 0 72 32 75 847.3 0 75 33 68 847.3 0 68 34 79 847.3 0 79 35 112 847.3 0 112 36 94 847.3 0 94 37 75 847.3 0 75 38 59 847.3 0 59 39 74 847.3 0 74 40 99 847.3 0 99 41 71 847.3 0 71 42 107 847.3 0 107 43 89 847.3 0 89 44 81 847.3 0 81 45 126 847.3 0 126 46 86 847.3 0 86 47 79 847.3 0 79 48 104 847.3 0 104 49 94 847.3 0 94 50 85 847.3 0 85 51 113 847.3 0 113 52 88 847.3 0 88 53 118 847.3 0 118 54 161 847.3 0 161 55 107 847.3 0 107 56 139 847.3 0 139 57 79 847.3 0 79 58 63 847.3 0 63 59 86 847.3 0 86 60 136 847.3 0 136 61 115 847.3 0 115 62 230 847.3 0 230 63 125 847.3 0 125 64 102 847.3 0 102 65 98 847.3 0 98 66 210 847.3 0 210 67 126 847.3 0 126 68 224 847.3 0 224 69 315 847.3 0 315 70 430 847.3 0 430 71 568 847.3 0 568 72 1165 847.3 0 1165 73 2059 847.3 0 2059 74 587 847.3 0 587 75 618 847.3 0 618 76 1210 847.3 0 1210 77 668 847.3 0 668 78 575 847.3 0 575 79 1480 847.3 0 1480 80 1241 847.3 0 1241 81 1159 847.3 0 1159 82 1249 847.3 0 1249 83 1883 847.3 0 1883 84 2564 847.3 0 2564 85 3849 847.3 0 3849 86 106661 847.3 0 106661 87 83748 847.3 0 83748 88 47302 847.3 0 47302 89 247 847.3 0 247 90 77 847.3 0 77 91 56 847.3 0 56 92 52 847.3 0 52 93 49 847.3 0 49 94 64 847.3 0 64 95 82 847.3 0 82 96 63 847.3 0 63 97 76 847.3 0 76 98 61 847.3 0 61 99 98 847.3 0 98 100 86 847.3 0 86 101 81 847.3 0 81 102 71 847.3 0 71 103 170 847.3 0 170 104 160 847.3 0 160 105 119 847.3 0 119 106 58 847.3 0 58 107 57 847.3 0 57 108 78 847.3 0 78 109 76 847.3 0 76 110 75 847.3 0 75 111 85 847.3 0 85 112 65 847.3 0 65 113 60 847.3 0 60 114 72 847.3 0 72 115 86 847.3 0 86 116 191 847.3 0 191 117 86 847.3 0 86 118 83 847.3 0 83 119 91 847.3 0 91 120 88 847.3 0 88 121 77 847.3 0 77 122 91 847.3 0 91 123 512 847.3 0 512 124 1820 847.3 0 1820 125 728 847.3 0 728 126 802 847.3 0 802 127 209 847.3 0 209 128 108 847.3 0 108 129 192 847.3 0 192 130 158 847.3 0 158 131 184 847.3 0 184 132 135 847.3 0 135 133 120 847.3 0 120 134 157 847.3 0 157 135 107 847.3 0 107 136 130 847.3 0 130 137 140 847.3 0 140 138 138 847.3 0 138 139 163 847.3 0 163 140 115 847.3 0 115 141 223 847.3 0 223 142 219 847.3 0 219 143 338 847.3 0 338 144 5722 847.3 0 5722 145 5502 847.3 0 5502 146 2557 847.3 0 2557 147 153 847.3 0 153 148 91 847.3 0 91 149 92 847.3 0 92 150 96 847.3 0 96 151 91 847.3 0 91 152 66 847.3 0 66 153 92 847.3 0 92 154 83 847.3 0 83 155 103 847.3 0 103 156 73 847.3 0 73 157 80 847.3 0 80 158 105 847.3 0 105 159 79 847.3 0 79 160 99 847.3 0 99 161 91 847.3 0 91 162 67 847.3 0 67 163 76 847.3 0 76 164 74 847.3 0 74 165 89 847.3 0 89 166 199 847.3 0 199 167 405 847.3 0 405 168 727 847.3 0 727 169 154 847.3 0 154 170 113 847.3 0 113 171 121 847.3 0 121 172 144 847.3 0 144 173 82 847.3 0 82 174 101 847.3 0 101 175 127 847.3 0 127 176 143 847.3 0 143 177 105 847.3 0 105 178 107 847.3 0 107 179 87 847.3 0 87 180 139 847.3 0 139 181 146 847.3 0 146 182 147 847.3 0 147 183 186 847.3 0 186 184 287 847.3 0 287 185 4794 847.3 0 4794 186 2910 847.3 0 2910 187 1367 847.3 0 1367 188 129 847.3 0 129 189 83 847.3 0 83 190 72 847.3 0 72 191 80 847.3 0 80 192 112 847.3 0 112 193 154 847.3 0 154 194 107 847.3 0 107 195 127 847.3 0 127 196 127 847.3 0 127 197 103 847.3 0 103 198 100 847.3 0 100 199 122 847.3 0 122 200 145 847.3 0 145 201 136 847.3 0 136 202 117 847.3 0 117 203 123 847.3 0 123 204 185 847.3 0 185 205 184 847.3 0 184 206 3177 847.3 0 3177 207 2486 847.3 0 2486 208 1084 847.3 0 1084 209 85 847.3 0 85 210 339 847.3 0 339 211 386 847.3 0 386 212 392 847.3 0 392 213 254 847.3 0 254 214 103 847.3 0 103 215 67 847.3 0 67 216 92 847.3 0 92 217 79 847.3 0 79 218 71 847.3 0 71 219 71 847.3 0 71 220 87 847.3 0 87 221 73 847.3 0 73 222 93 847.3 0 93 223 71 847.3 0 71 224 87 847.3 0 87 225 91 847.3 0 91 226 109 847.3 0 109 227 129 847.3 0 129 228 163 847.3 0 163 229 70 847.3 0 70 230 56 847.3 0 56 231 102 847.3 0 102 232 56 847.3 0 56 233 66 847.3 0 66 234 44 847.3 0 44 235 79 847.3 0 79 236 61 847.3 0 61 237 65 847.3 0 65 238 53 847.3 0 53 239 52 847.3 0 52 240 58 847.3 0 58 241 89 847.3 0 89 242 80 847.3 0 80 243 68 847.3 0 68 244 54 847.3 0 54 245 69 847.3 0 69 246 85 847.3 0 85 247 87 847.3 0 87 248 82 847.3 0 82 249 62 847.3 0 62 250 74 847.3 0 74 251 51 847.3 0 51 252 74 847.3 0 74 253 60 847.3 0 60 254 52 847.3 0 52 255 169 847.3 0 169 256 74 847.3 0 74 257 120 847.3 0 120 258 61 847.3 0 61 259 204 847.3 0 204 260 135 847.3 0 135 261 144 847.3 0 144 262 82 847.3 0 82 263 71 847.3 0 71 264 66 847.3 0 66 265 50 847.3 0 50 266 81 847.3 0 81 267 110 847.3 0 110 268 109 847.3 0 109 269 100 847.3 0 100 270 86 847.3 0 86 271 64 847.3 0 64 272 73 847.3 0 73 273 76 847.3 0 76 274 76 847.3 0 76 275 94 847.3 0 94 276 84 847.3 0 84 277 71 847.3 0 71 278 53 847.3 0 53 279 88 847.3 0 88 280 71 847.3 0 71 281 105 847.3 0 105 282 100 847.3 0 100 283 62 847.3 0 62 284 58 847.3 0 58 285 68 847.3 0 68 286 58 847.3 0 58 287 69 847.3 0 69 288 91 847.3 0 91 289 68 847.3 0 68 290 59 847.3 0 59 291 68 847.3 0 68 292 66 847.3 0 66 293 74 847.3 0 74 294 115 847.3 0 115 295 127 847.3 0 127 296 163 847.3 0 163 297 113 847.3 0 113 298 66 847.3 0 66 299 65 847.3 0 65 300 152 847.3 0 152 301 194 847.3 0 194 Finished in 19.35 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 304,945 Reads with adapters: 122 (0.0%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 73 (0.0%) Total basepairs processed: 63,153,009 bp Total written (filtered): 16,789 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 122 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 2 0.0 3 0 2 20 44 0.0 4 42 2 22 1 0.0 4 1 24 1 0.0 4 1 48 4 0.0 4 0 0 0 0 4 49 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 17 0.0 4 0 0 0 0 17 76 1 0.0 4 1 79 1 0.0 4 1 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 137 1 0.0 4 1 145 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 149 16 0.0 4 0 0 0 0 16 150 4 0.0 4 0 0 0 0 4 154 2 0.0 4 0 0 0 0 2 155 3 0.0 4 0 0 0 0 3 156 3 0.0 4 0 0 0 0 3 166 1 0.0 4 1 168 1 0.0 4 0 1 169 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 198 1 0.0 4 1 200 1 0.0 4 0 0 1 209 2 0.0 4 0 0 0 0 2 248 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (137 us/read; 0.44 M reads/minute). === Summary === Total reads processed: 73 Reads with adapters: 1 (1.4%) Reads written (passing filters): 73 (100.0%) Total basepairs processed: 16,789 bp Total written (filtered): 16,778 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 0.0% T: 100.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 0 1