This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 162.45 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 198,613 (5.7%) Reads that were too short: 39,686 (1.1%) Reads written (passing filters): 158,927 (4.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 38,351,048 bp (3.7%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 198613 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4704 847.3 0 4704 7 3772 847.3 0 3772 8 41906 847.3 0 41906 9 33894 847.3 0 33894 10 213 847.3 0 213 11 96 847.3 0 96 12 109 847.3 0 109 13 96 847.3 0 96 14 102 847.3 0 102 15 164 847.3 0 164 16 127 847.3 0 127 17 123 847.3 0 123 18 111 847.3 0 111 19 144 847.3 0 144 20 89 847.3 0 89 21 125 847.3 0 125 22 110 847.3 0 110 23 128 847.3 0 128 24 128 847.3 0 128 25 126 847.3 0 126 26 129 847.3 0 129 27 157 847.3 0 157 28 123 847.3 0 123 29 121 847.3 0 121 30 146 847.3 0 146 31 129 847.3 0 129 32 172 847.3 0 172 33 203 847.3 0 203 34 120 847.3 0 120 35 206 847.3 0 206 36 190 847.3 0 190 37 2220 847.3 0 2220 38 1688 847.3 0 1688 39 1714 847.3 0 1714 40 394 847.3 0 394 41 163 847.3 0 163 42 124 847.3 0 124 43 149 847.3 0 149 44 150 847.3 0 150 45 156 847.3 0 156 46 133 847.3 0 133 47 137 847.3 0 137 48 131 847.3 0 131 49 124 847.3 0 124 50 93 847.3 0 93 51 145 847.3 0 145 52 130 847.3 0 130 53 101 847.3 0 101 54 116 847.3 0 116 55 107 847.3 0 107 56 125 847.3 0 125 57 135 847.3 0 135 58 114 847.3 0 114 59 114 847.3 0 114 60 121 847.3 0 121 61 98 847.3 0 98 62 108 847.3 0 108 63 124 847.3 0 124 64 98 847.3 0 98 65 108 847.3 0 108 66 119 847.3 0 119 67 115 847.3 0 115 68 124 847.3 0 124 69 128 847.3 0 128 70 133 847.3 0 133 71 133 847.3 0 133 72 144 847.3 0 144 73 114 847.3 0 114 74 146 847.3 0 146 75 175 847.3 0 175 76 188 847.3 0 188 77 310 847.3 0 310 78 303 847.3 0 303 79 190 847.3 0 190 80 237 847.3 0 237 81 131 847.3 0 131 82 148 847.3 0 148 83 146 847.3 0 146 84 113 847.3 0 113 85 120 847.3 0 120 86 119 847.3 0 119 87 138 847.3 0 138 88 246 847.3 0 246 89 189 847.3 0 189 90 221 847.3 0 221 91 457 847.3 0 457 92 1079 847.3 0 1079 93 1516 847.3 0 1516 94 278 847.3 0 278 95 365 847.3 0 365 96 136 847.3 0 136 97 125 847.3 0 125 98 122 847.3 0 122 99 118 847.3 0 118 100 124 847.3 0 124 101 90 847.3 0 90 102 102 847.3 0 102 103 113 847.3 0 113 104 200 847.3 0 200 105 277 847.3 0 277 106 263 847.3 0 263 107 148 847.3 0 148 108 141 847.3 0 141 109 149 847.3 0 149 110 161 847.3 0 161 111 122 847.3 0 122 112 321 847.3 0 321 113 330 847.3 0 330 114 261 847.3 0 261 115 265 847.3 0 265 116 251 847.3 0 251 117 159 847.3 0 159 118 189 847.3 0 189 119 156 847.3 0 156 120 140 847.3 0 140 121 149 847.3 0 149 122 193 847.3 0 193 123 151 847.3 0 151 124 201 847.3 0 201 125 156 847.3 0 156 126 181 847.3 0 181 127 211 847.3 0 211 128 440 847.3 0 440 129 2667 847.3 0 2667 130 956 847.3 0 956 131 920 847.3 0 920 132 222 847.3 0 222 133 235 847.3 0 235 134 223 847.3 0 223 135 247 847.3 0 247 136 285 847.3 0 285 137 330 847.3 0 330 138 312 847.3 0 312 139 457 847.3 0 457 140 537 847.3 0 537 141 1294 847.3 0 1294 142 21487 847.3 0 21487 143 6646 847.3 0 6646 144 3913 847.3 0 3913 145 101 847.3 0 101 146 139 847.3 0 139 147 120 847.3 0 120 148 143 847.3 0 143 149 334 847.3 0 334 150 302 847.3 0 302 151 199 847.3 0 199 152 112 847.3 0 112 153 142 847.3 0 142 154 142 847.3 0 142 155 117 847.3 0 117 156 132 847.3 0 132 157 135 847.3 0 135 158 117 847.3 0 117 159 120 847.3 0 120 160 103 847.3 0 103 161 91 847.3 0 91 162 125 847.3 0 125 163 144 847.3 0 144 164 114 847.3 0 114 165 103 847.3 0 103 166 138 847.3 0 138 167 114 847.3 0 114 168 95 847.3 0 95 169 137 847.3 0 137 170 169 847.3 0 169 171 98 847.3 0 98 172 85 847.3 0 85 173 134 847.3 0 134 174 109 847.3 0 109 175 103 847.3 0 103 176 95 847.3 0 95 177 119 847.3 0 119 178 140 847.3 0 140 179 130 847.3 0 130 180 110 847.3 0 110 181 80 847.3 0 80 182 132 847.3 0 132 183 114 847.3 0 114 184 75 847.3 0 75 185 101 847.3 0 101 186 135 847.3 0 135 187 95 847.3 0 95 188 125 847.3 0 125 189 110 847.3 0 110 190 109 847.3 0 109 191 124 847.3 0 124 192 114 847.3 0 114 193 91 847.3 0 91 194 120 847.3 0 120 195 96 847.3 0 96 196 117 847.3 0 117 197 151 847.3 0 151 198 95 847.3 0 95 199 94 847.3 0 94 200 119 847.3 0 119 201 135 847.3 0 135 202 147 847.3 0 147 203 132 847.3 0 132 204 176 847.3 0 176 205 165 847.3 0 165 206 119 847.3 0 119 207 151 847.3 0 151 208 154 847.3 0 154 209 553 847.3 0 553 210 436 847.3 0 436 211 252 847.3 0 252 212 266 847.3 0 266 213 208 847.3 0 208 214 1631 847.3 0 1631 215 1376 847.3 0 1376 216 1981 847.3 0 1981 217 224 847.3 0 224 218 149 847.3 0 149 219 133 847.3 0 133 220 132 847.3 0 132 221 214 847.3 0 214 222 153 847.3 0 153 223 116 847.3 0 116 224 214 847.3 0 214 225 174 847.3 0 174 226 249 847.3 0 249 227 277 847.3 0 277 228 248 847.3 0 248 229 657 847.3 0 657 230 503 847.3 0 503 231 474 847.3 0 474 232 331 847.3 0 331 233 503 847.3 0 503 234 5293 847.3 0 5293 235 5916 847.3 0 5916 236 3983 847.3 0 3983 237 973 847.3 0 973 238 104 847.3 0 104 239 105 847.3 0 105 240 88 847.3 0 88 241 97 847.3 0 97 242 78 847.3 0 78 243 111 847.3 0 111 244 104 847.3 0 104 245 88 847.3 0 88 246 121 847.3 0 121 247 89 847.3 0 89 248 76 847.3 0 76 249 90 847.3 0 90 250 102 847.3 0 102 251 105 847.3 0 105 252 108 847.3 0 108 253 193 847.3 0 193 254 312 847.3 0 312 255 267 847.3 0 267 256 166 847.3 0 166 257 93 847.3 0 93 258 103 847.3 0 103 259 110 847.3 0 110 260 116 847.3 0 116 261 139 847.3 0 139 262 503 847.3 0 503 263 417 847.3 0 417 264 520 847.3 0 520 265 323 847.3 0 323 266 149 847.3 0 149 267 81 847.3 0 81 268 88 847.3 0 88 269 80 847.3 0 80 270 120 847.3 0 120 271 141 847.3 0 141 272 131 847.3 0 131 273 79 847.3 0 79 274 99 847.3 0 99 275 121 847.3 0 121 276 139 847.3 0 139 277 188 847.3 0 188 278 106 847.3 0 106 279 76 847.3 0 76 280 86 847.3 0 86 281 114 847.3 0 114 282 97 847.3 0 97 283 102 847.3 0 102 284 145 847.3 0 145 285 120 847.3 0 120 286 89 847.3 0 89 287 75 847.3 0 75 288 87 847.3 0 87 289 63 847.3 0 63 290 62 847.3 0 62 291 246 847.3 0 246 292 236 847.3 0 236 293 208 847.3 0 208 294 113 847.3 0 113 295 272 847.3 0 272 296 481 847.3 0 481 297 845 847.3 0 845 298 555 847.3 0 555 299 329 847.3 0 329 300 93 847.3 0 93 301 279 847.3 0 279 Finished in 10.58 s (67 us/read; 0.90 M reads/minute). === Summary === Total reads processed: 158,927 Reads with adapters: 45,982 (28.9%) Reads that were too short: 81 (0.1%) Reads written (passing filters): 45,901 (28.9%) Total basepairs processed: 38,351,048 bp Total written (filtered): 12,650,382 bp (33.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 45982 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 19 0.0 3 0 0 6 13 16 388 0.0 3 0 321 41 26 17 41500 0.0 3 39219 1872 329 80 18 3406 0.0 3 3105 227 39 35 19 113 0.0 3 2 9 69 33 20 309 0.0 3 173 5 6 125 21 7 0.0 3 1 0 0 6 23 1 0.0 3 0 0 0 1 25 1 0.0 3 1 26 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 42 7 0.0 3 0 0 0 7 43 3 0.0 3 0 0 0 3 45 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 47 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 50 42 0.0 3 0 0 1 41 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 6 0.0 3 0 0 0 6 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 63 13 0.0 3 0 0 0 13 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 2 0.0 3 0 0 1 1 78 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 3 0.0 3 0 0 0 3 101 11 0.0 3 0 0 0 11 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 1 1 151 5 0.0 3 0 0 0 5 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 6 0.0 3 0 0 0 6 155 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 182 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 283 3 0.0 3 0 0 0 3 286 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.68 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 45,901 Reads with adapters: 1,080 (2.4%) Reads written (passing filters): 45,901 (100.0%) Total basepairs processed: 12,650,382 bp Total written (filtered): 12,647,070 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1080 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.6% C: 13.0% G: 8.7% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1017 717.2 0 1017 4 55 179.3 0 55 5 7 44.8 0 7 6 1 11.2 0 1