This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 156.68 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 93,722 (2.7%) Reads that were too short: 15,085 (0.4%) Reads written (passing filters): 78,637 (2.3%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 19,620,646 bp (1.9%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 93722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 762 847.3 0 762 7 27063 847.3 0 27063 8 22786 847.3 0 22786 9 67 847.3 0 67 10 51 847.3 0 51 11 28 847.3 0 28 12 48 847.3 0 48 13 40 847.3 0 40 14 36 847.3 0 36 15 25 847.3 0 25 16 44 847.3 0 44 17 45 847.3 0 45 18 48 847.3 0 48 19 46 847.3 0 46 20 52 847.3 0 52 21 50 847.3 0 50 22 77 847.3 0 77 23 44 847.3 0 44 24 40 847.3 0 40 25 44 847.3 0 44 26 46 847.3 0 46 27 116 847.3 0 116 28 56 847.3 0 56 29 173 847.3 0 173 30 131 847.3 0 131 31 204 847.3 0 204 32 69 847.3 0 69 33 41 847.3 0 41 34 50 847.3 0 50 35 23 847.3 0 23 36 43 847.3 0 43 37 59 847.3 0 59 38 54 847.3 0 54 39 40 847.3 0 40 40 53 847.3 0 53 41 42 847.3 0 42 42 33 847.3 0 33 43 30 847.3 0 30 44 54 847.3 0 54 45 49 847.3 0 49 46 60 847.3 0 60 47 46 847.3 0 46 48 25 847.3 0 25 49 43 847.3 0 43 50 33 847.3 0 33 51 33 847.3 0 33 52 69 847.3 0 69 53 48 847.3 0 48 54 234 847.3 0 234 55 412 847.3 0 412 56 225 847.3 0 225 57 36 847.3 0 36 58 38 847.3 0 38 59 35 847.3 0 35 60 44 847.3 0 44 61 102 847.3 0 102 62 267 847.3 0 267 63 320 847.3 0 320 64 46 847.3 0 46 65 49 847.3 0 49 66 31 847.3 0 31 67 87 847.3 0 87 68 91 847.3 0 91 69 241 847.3 0 241 70 48 847.3 0 48 71 228 847.3 0 228 72 177 847.3 0 177 73 103 847.3 0 103 74 78 847.3 0 78 75 47 847.3 0 47 76 50 847.3 0 50 77 64 847.3 0 64 78 53 847.3 0 53 79 86 847.3 0 86 80 60 847.3 0 60 81 69 847.3 0 69 82 68 847.3 0 68 83 139 847.3 0 139 84 99 847.3 0 99 85 67 847.3 0 67 86 138 847.3 0 138 87 129 847.3 0 129 88 66 847.3 0 66 89 38 847.3 0 38 90 61 847.3 0 61 91 43 847.3 0 43 92 73 847.3 0 73 93 43 847.3 0 43 94 54 847.3 0 54 95 69 847.3 0 69 96 80 847.3 0 80 97 68 847.3 0 68 98 67 847.3 0 67 99 39 847.3 0 39 100 47 847.3 0 47 101 41 847.3 0 41 102 36 847.3 0 36 103 31 847.3 0 31 104 61 847.3 0 61 105 77 847.3 0 77 106 42 847.3 0 42 107 35 847.3 0 35 108 40 847.3 0 40 109 46 847.3 0 46 110 58 847.3 0 58 111 56 847.3 0 56 112 100 847.3 0 100 113 101 847.3 0 101 114 84 847.3 0 84 115 83 847.3 0 83 116 77 847.3 0 77 117 52 847.3 0 52 118 48 847.3 0 48 119 86 847.3 0 86 120 71 847.3 0 71 121 91 847.3 0 91 122 124 847.3 0 124 123 281 847.3 0 281 124 390 847.3 0 390 125 504 847.3 0 504 126 1203 847.3 0 1203 127 1280 847.3 0 1280 128 2295 847.3 0 2295 129 848 847.3 0 848 130 449 847.3 0 449 131 168 847.3 0 168 132 112 847.3 0 112 133 78 847.3 0 78 134 117 847.3 0 117 135 109 847.3 0 109 136 90 847.3 0 90 137 57 847.3 0 57 138 85 847.3 0 85 139 80 847.3 0 80 140 66 847.3 0 66 141 69 847.3 0 69 142 93 847.3 0 93 143 57 847.3 0 57 144 223 847.3 0 223 145 261 847.3 0 261 146 90 847.3 0 90 147 70 847.3 0 70 148 89 847.3 0 89 149 69 847.3 0 69 150 52 847.3 0 52 151 78 847.3 0 78 152 49 847.3 0 49 153 32 847.3 0 32 154 66 847.3 0 66 155 51 847.3 0 51 156 67 847.3 0 67 157 100 847.3 0 100 158 113 847.3 0 113 159 71 847.3 0 71 160 60 847.3 0 60 161 59 847.3 0 59 162 57 847.3 0 57 163 69 847.3 0 69 164 62 847.3 0 62 165 132 847.3 0 132 166 321 847.3 0 321 167 209 847.3 0 209 168 413 847.3 0 413 169 62 847.3 0 62 170 45 847.3 0 45 171 64 847.3 0 64 172 59 847.3 0 59 173 61 847.3 0 61 174 79 847.3 0 79 175 77 847.3 0 77 176 93 847.3 0 93 177 51 847.3 0 51 178 68 847.3 0 68 179 67 847.3 0 67 180 62 847.3 0 62 181 78 847.3 0 78 182 79 847.3 0 79 183 99 847.3 0 99 184 93 847.3 0 93 185 149 847.3 0 149 186 113 847.3 0 113 187 92 847.3 0 92 188 63 847.3 0 63 189 75 847.3 0 75 190 95 847.3 0 95 191 92 847.3 0 92 192 113 847.3 0 113 193 142 847.3 0 142 194 190 847.3 0 190 195 1400 847.3 0 1400 196 1247 847.3 0 1247 197 1481 847.3 0 1481 198 578 847.3 0 578 199 872 847.3 0 872 200 163 847.3 0 163 201 73 847.3 0 73 202 61 847.3 0 61 203 54 847.3 0 54 204 65 847.3 0 65 205 56 847.3 0 56 206 73 847.3 0 73 207 69 847.3 0 69 208 77 847.3 0 77 209 67 847.3 0 67 210 112 847.3 0 112 211 172 847.3 0 172 212 128 847.3 0 128 213 126 847.3 0 126 214 115 847.3 0 115 215 84 847.3 0 84 216 68 847.3 0 68 217 71 847.3 0 71 218 237 847.3 0 237 219 279 847.3 0 279 220 419 847.3 0 419 221 274 847.3 0 274 222 111 847.3 0 111 223 100 847.3 0 100 224 67 847.3 0 67 225 86 847.3 0 86 226 89 847.3 0 89 227 87 847.3 0 87 228 87 847.3 0 87 229 79 847.3 0 79 230 70 847.3 0 70 231 108 847.3 0 108 232 108 847.3 0 108 233 83 847.3 0 83 234 67 847.3 0 67 235 101 847.3 0 101 236 99 847.3 0 99 237 99 847.3 0 99 238 262 847.3 0 262 239 282 847.3 0 282 240 325 847.3 0 325 241 177 847.3 0 177 242 172 847.3 0 172 243 122 847.3 0 122 244 112 847.3 0 112 245 337 847.3 0 337 246 167 847.3 0 167 247 240 847.3 0 240 248 112 847.3 0 112 249 142 847.3 0 142 250 117 847.3 0 117 251 94 847.3 0 94 252 86 847.3 0 86 253 96 847.3 0 96 254 367 847.3 0 367 255 312 847.3 0 312 256 339 847.3 0 339 257 103 847.3 0 103 258 87 847.3 0 87 259 82 847.3 0 82 260 107 847.3 0 107 261 87 847.3 0 87 262 302 847.3 0 302 263 283 847.3 0 283 264 169 847.3 0 169 265 107 847.3 0 107 266 140 847.3 0 140 267 764 847.3 0 764 268 816 847.3 0 816 269 576 847.3 0 576 270 196 847.3 0 196 271 71 847.3 0 71 272 114 847.3 0 114 273 265 847.3 0 265 274 325 847.3 0 325 275 183 847.3 0 183 276 116 847.3 0 116 277 120 847.3 0 120 278 93 847.3 0 93 279 88 847.3 0 88 280 83 847.3 0 83 281 149 847.3 0 149 282 108 847.3 0 108 283 78 847.3 0 78 284 64 847.3 0 64 285 49 847.3 0 49 286 75 847.3 0 75 287 112 847.3 0 112 288 146 847.3 0 146 289 89 847.3 0 89 290 90 847.3 0 90 291 70 847.3 0 70 292 66 847.3 0 66 293 84 847.3 0 84 294 106 847.3 0 106 295 110 847.3 0 110 296 70 847.3 0 70 297 91 847.3 0 91 298 80 847.3 0 80 299 73 847.3 0 73 300 96 847.3 0 96 301 93 847.3 0 93 Finished in 5.30 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 78,637 Reads with adapters: 26,938 (34.3%) Reads that were too short: 140 (0.2%) Reads written (passing filters): 26,798 (34.1%) Total basepairs processed: 19,620,646 bp Total written (filtered): 7,388,495 bp (37.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26938 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 5 5 16 273 0.0 3 0 246 25 2 17 25961 0.0 3 24448 1293 181 39 18 60 0.0 3 5 49 5 1 19 51 0.0 3 0 8 23 20 20 227 0.0 3 185 13 2 27 22 2 0.0 3 0 0 1 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 16 0.0 3 0 0 0 16 44 2 0.0 3 0 0 0 2 45 5 0.0 3 0 0 1 4 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 50 4 0.0 3 0 0 1 3 53 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 61 6 0.0 3 0 0 0 6 63 62 0.0 3 0 0 0 62 64 2 0.0 3 0 0 1 1 65 10 0.0 3 0 0 0 10 66 50 0.0 3 0 0 0 50 68 9 0.0 3 0 0 0 9 69 6 0.0 3 0 0 1 5 71 1 0.0 3 0 0 0 1 75 10 0.0 3 0 0 0 10 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 3 0.0 3 0 0 1 2 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 137 6 0.0 3 0 0 0 6 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 1 1 160 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 3 0.0 3 0 0 0 3 164 5 0.0 3 0 0 0 5 165 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 66 0.0 3 0 0 3 63 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 26,798 Reads with adapters: 114 (0.4%) Reads written (passing filters): 26,798 (100.0%) Total basepairs processed: 7,388,495 bp Total written (filtered): 7,388,128 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 114 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.6% C: 38.6% G: 19.3% T: 10.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 98 418.7 0 98 4 11 104.7 0 11 5 1 26.2 0 1 6 4 6.5 0 4