This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 163.94 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 89,674 (2.6%) Reads that were too short: 12,510 (0.4%) Reads written (passing filters): 77,164 (2.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 19,465,124 bp (1.9%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 89674 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 550 847.3 0 550 7 24610 847.3 0 24610 8 27278 847.3 0 27278 9 64 847.3 0 64 10 37 847.3 0 37 11 29 847.3 0 29 12 40 847.3 0 40 13 37 847.3 0 37 14 30 847.3 0 30 15 40 847.3 0 40 16 49 847.3 0 49 17 33 847.3 0 33 18 60 847.3 0 60 19 39 847.3 0 39 20 37 847.3 0 37 21 46 847.3 0 46 22 43 847.3 0 43 23 26 847.3 0 26 24 21 847.3 0 21 25 31 847.3 0 31 26 24 847.3 0 24 27 47 847.3 0 47 28 29 847.3 0 29 29 40 847.3 0 40 30 33 847.3 0 33 31 36 847.3 0 36 32 31 847.3 0 31 33 43 847.3 0 43 34 30 847.3 0 30 35 27 847.3 0 27 36 32 847.3 0 32 37 67 847.3 0 67 38 40 847.3 0 40 39 50 847.3 0 50 40 52 847.3 0 52 41 40 847.3 0 40 42 49 847.3 0 49 43 24 847.3 0 24 44 64 847.3 0 64 45 45 847.3 0 45 46 70 847.3 0 70 47 51 847.3 0 51 48 35 847.3 0 35 49 42 847.3 0 42 50 33 847.3 0 33 51 37 847.3 0 37 52 88 847.3 0 88 53 43 847.3 0 43 54 167 847.3 0 167 55 319 847.3 0 319 56 190 847.3 0 190 57 37 847.3 0 37 58 33 847.3 0 33 59 51 847.3 0 51 60 40 847.3 0 40 61 115 847.3 0 115 62 227 847.3 0 227 63 271 847.3 0 271 64 34 847.3 0 34 65 48 847.3 0 48 66 42 847.3 0 42 67 94 847.3 0 94 68 95 847.3 0 95 69 196 847.3 0 196 70 35 847.3 0 35 71 49 847.3 0 49 72 48 847.3 0 48 73 32 847.3 0 32 74 41 847.3 0 41 75 39 847.3 0 39 76 60 847.3 0 60 77 79 847.3 0 79 78 92 847.3 0 92 79 60 847.3 0 60 80 82 847.3 0 82 81 44 847.3 0 44 82 29 847.3 0 29 83 75 847.3 0 75 84 37 847.3 0 37 85 49 847.3 0 49 86 68 847.3 0 68 87 78 847.3 0 78 88 80 847.3 0 80 89 70 847.3 0 70 90 79 847.3 0 79 91 74 847.3 0 74 92 51 847.3 0 51 93 53 847.3 0 53 94 51 847.3 0 51 95 56 847.3 0 56 96 96 847.3 0 96 97 96 847.3 0 96 98 85 847.3 0 85 99 65 847.3 0 65 100 69 847.3 0 69 101 55 847.3 0 55 102 46 847.3 0 46 103 49 847.3 0 49 104 48 847.3 0 48 105 82 847.3 0 82 106 49 847.3 0 49 107 48 847.3 0 48 108 49 847.3 0 49 109 47 847.3 0 47 110 40 847.3 0 40 111 56 847.3 0 56 112 79 847.3 0 79 113 73 847.3 0 73 114 54 847.3 0 54 115 90 847.3 0 90 116 79 847.3 0 79 117 47 847.3 0 47 118 59 847.3 0 59 119 56 847.3 0 56 120 69 847.3 0 69 121 70 847.3 0 70 122 89 847.3 0 89 123 227 847.3 0 227 124 281 847.3 0 281 125 452 847.3 0 452 126 1123 847.3 0 1123 127 1118 847.3 0 1118 128 1976 847.3 0 1976 129 571 847.3 0 571 130 288 847.3 0 288 131 153 847.3 0 153 132 104 847.3 0 104 133 72 847.3 0 72 134 93 847.3 0 93 135 104 847.3 0 104 136 71 847.3 0 71 137 34 847.3 0 34 138 68 847.3 0 68 139 55 847.3 0 55 140 61 847.3 0 61 141 50 847.3 0 50 142 84 847.3 0 84 143 57 847.3 0 57 144 238 847.3 0 238 145 238 847.3 0 238 146 73 847.3 0 73 147 58 847.3 0 58 148 66 847.3 0 66 149 70 847.3 0 70 150 51 847.3 0 51 151 49 847.3 0 49 152 58 847.3 0 58 153 36 847.3 0 36 154 52 847.3 0 52 155 69 847.3 0 69 156 46 847.3 0 46 157 101 847.3 0 101 158 102 847.3 0 102 159 69 847.3 0 69 160 50 847.3 0 50 161 44 847.3 0 44 162 60 847.3 0 60 163 55 847.3 0 55 164 42 847.3 0 42 165 108 847.3 0 108 166 169 847.3 0 169 167 105 847.3 0 105 168 178 847.3 0 178 169 55 847.3 0 55 170 38 847.3 0 38 171 62 847.3 0 62 172 47 847.3 0 47 173 59 847.3 0 59 174 81 847.3 0 81 175 54 847.3 0 54 176 113 847.3 0 113 177 67 847.3 0 67 178 47 847.3 0 47 179 51 847.3 0 51 180 78 847.3 0 78 181 61 847.3 0 61 182 91 847.3 0 91 183 106 847.3 0 106 184 100 847.3 0 100 185 112 847.3 0 112 186 101 847.3 0 101 187 107 847.3 0 107 188 60 847.3 0 60 189 63 847.3 0 63 190 84 847.3 0 84 191 77 847.3 0 77 192 97 847.3 0 97 193 123 847.3 0 123 194 171 847.3 0 171 195 1441 847.3 0 1441 196 1228 847.3 0 1228 197 1694 847.3 0 1694 198 663 847.3 0 663 199 1057 847.3 0 1057 200 156 847.3 0 156 201 74 847.3 0 74 202 63 847.3 0 63 203 60 847.3 0 60 204 76 847.3 0 76 205 81 847.3 0 81 206 66 847.3 0 66 207 79 847.3 0 79 208 67 847.3 0 67 209 51 847.3 0 51 210 80 847.3 0 80 211 94 847.3 0 94 212 113 847.3 0 113 213 80 847.3 0 80 214 106 847.3 0 106 215 74 847.3 0 74 216 66 847.3 0 66 217 53 847.3 0 53 218 209 847.3 0 209 219 208 847.3 0 208 220 311 847.3 0 311 221 214 847.3 0 214 222 140 847.3 0 140 223 105 847.3 0 105 224 77 847.3 0 77 225 93 847.3 0 93 226 88 847.3 0 88 227 87 847.3 0 87 228 76 847.3 0 76 229 69 847.3 0 69 230 76 847.3 0 76 231 75 847.3 0 75 232 79 847.3 0 79 233 87 847.3 0 87 234 66 847.3 0 66 235 91 847.3 0 91 236 78 847.3 0 78 237 84 847.3 0 84 238 244 847.3 0 244 239 278 847.3 0 278 240 285 847.3 0 285 241 143 847.3 0 143 242 113 847.3 0 113 243 76 847.3 0 76 244 72 847.3 0 72 245 345 847.3 0 345 246 163 847.3 0 163 247 201 847.3 0 201 248 90 847.3 0 90 249 126 847.3 0 126 250 113 847.3 0 113 251 75 847.3 0 75 252 81 847.3 0 81 253 76 847.3 0 76 254 476 847.3 0 476 255 384 847.3 0 384 256 461 847.3 0 461 257 81 847.3 0 81 258 86 847.3 0 86 259 63 847.3 0 63 260 57 847.3 0 57 261 97 847.3 0 97 262 398 847.3 0 398 263 348 847.3 0 348 264 237 847.3 0 237 265 72 847.3 0 72 266 108 847.3 0 108 267 422 847.3 0 422 268 411 847.3 0 411 269 273 847.3 0 273 270 119 847.3 0 119 271 45 847.3 0 45 272 93 847.3 0 93 273 192 847.3 0 192 274 195 847.3 0 195 275 108 847.3 0 108 276 72 847.3 0 72 277 79 847.3 0 79 278 78 847.3 0 78 279 54 847.3 0 54 280 63 847.3 0 63 281 89 847.3 0 89 282 76 847.3 0 76 283 78 847.3 0 78 284 61 847.3 0 61 285 41 847.3 0 41 286 54 847.3 0 54 287 74 847.3 0 74 288 89 847.3 0 89 289 75 847.3 0 75 290 56 847.3 0 56 291 59 847.3 0 59 292 57 847.3 0 57 293 53 847.3 0 53 294 74 847.3 0 74 295 85 847.3 0 85 296 79 847.3 0 79 297 60 847.3 0 60 298 61 847.3 0 61 299 59 847.3 0 59 300 72 847.3 0 72 301 83 847.3 0 83 Finished in 5.84 s (76 us/read; 0.79 M reads/minute). === Summary === Total reads processed: 77,164 Reads with adapters: 28,080 (36.4%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 28,063 (36.4%) Total basepairs processed: 19,465,124 bp Total written (filtered): 7,657,332 bp (39.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28080 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 16 0.0 3 0 0 15 1 19 382 0.0 3 0 373 8 1 20 27519 0.0 4 27020 475 22 1 1 21 66 0.0 4 7 58 0 0 1 22 13 0.0 4 4 1 5 1 2 23 20 0.0 4 0 0 0 15 5 24 2 0.0 4 0 0 0 0 2 38 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 1 76 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 27 0.0 4 0 0 0 0 27 130 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 154 6 0.0 4 0 0 0 0 6 171 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.63 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 28,063 Reads with adapters: 2,562 (9.1%) Reads written (passing filters): 28,063 (100.0%) Total basepairs processed: 7,657,332 bp Total written (filtered): 7,628,324 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2562 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 1.6% G: 96.4% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 438.5 0 7 4 2 109.6 0 2 8 2 0.4 0 2 9 50 0.1 0 3 47 10 461 0.0 1 13 448 11 2026 0.0 1 8 2018 12 3 0.0 1 0 3 14 1 0.0 1 0 1 15 1 0.0 1 0 1 151 1 0.0 1 0 1 152 1 0.0 1 0 1 170 6 0.0 1 6 229 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.