This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 162.30 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 49,800 (1.4%) Reads that were too short: 25,364 (0.7%) Reads written (passing filters): 24,436 (0.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 4,298,283 bp (0.4%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 49800 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 648 847.3 0 648 7 64 847.3 0 64 8 212 847.3 0 212 9 44 847.3 0 44 10 38 847.3 0 38 11 32 847.3 0 32 12 18 847.3 0 18 13 17 847.3 0 17 14 14 847.3 0 14 15 15 847.3 0 15 16 20 847.3 0 20 17 14 847.3 0 14 18 20 847.3 0 20 19 3 847.3 0 3 20 14 847.3 0 14 21 38 847.3 0 38 22 20 847.3 0 20 23 29 847.3 0 29 24 11 847.3 0 11 25 7 847.3 0 7 26 18 847.3 0 18 27 9 847.3 0 9 28 9 847.3 0 9 29 15 847.3 0 15 30 11 847.3 0 11 31 15 847.3 0 15 32 13 847.3 0 13 33 19 847.3 0 19 34 15 847.3 0 15 35 23 847.3 0 23 36 36 847.3 0 36 37 14 847.3 0 14 38 19 847.3 0 19 39 17 847.3 0 17 40 47 847.3 0 47 41 122 847.3 0 122 42 63 847.3 0 63 43 36 847.3 0 36 44 13 847.3 0 13 45 20 847.3 0 20 46 17 847.3 0 17 47 25 847.3 0 25 48 18 847.3 0 18 49 15 847.3 0 15 50 21 847.3 0 21 51 29 847.3 0 29 52 31 847.3 0 31 53 33 847.3 0 33 54 23 847.3 0 23 55 19 847.3 0 19 56 27 847.3 0 27 57 29 847.3 0 29 58 23 847.3 0 23 59 12 847.3 0 12 60 17 847.3 0 17 61 25 847.3 0 25 62 24 847.3 0 24 63 25 847.3 0 25 64 16 847.3 0 16 65 28 847.3 0 28 66 28 847.3 0 28 67 22 847.3 0 22 68 30 847.3 0 30 69 85 847.3 0 85 70 101 847.3 0 101 71 372 847.3 0 372 72 24 847.3 0 24 73 18 847.3 0 18 74 28 847.3 0 28 75 52 847.3 0 52 76 55 847.3 0 55 77 25 847.3 0 25 78 44 847.3 0 44 79 25 847.3 0 25 80 38 847.3 0 38 81 48 847.3 0 48 82 59 847.3 0 59 83 33 847.3 0 33 84 43 847.3 0 43 85 59 847.3 0 59 86 92 847.3 0 92 87 215 847.3 0 215 88 158 847.3 0 158 89 150 847.3 0 150 90 136 847.3 0 136 91 142 847.3 0 142 92 91 847.3 0 91 93 83 847.3 0 83 94 112 847.3 0 112 95 84 847.3 0 84 96 109 847.3 0 109 97 133 847.3 0 133 98 123 847.3 0 123 99 104 847.3 0 104 100 1095 847.3 0 1095 101 1594 847.3 0 1594 102 2146 847.3 0 2146 103 262 847.3 0 262 104 73 847.3 0 73 105 45 847.3 0 45 106 47 847.3 0 47 107 34 847.3 0 34 108 39 847.3 0 39 109 36 847.3 0 36 110 49 847.3 0 49 111 44 847.3 0 44 112 41 847.3 0 41 113 58 847.3 0 58 114 59 847.3 0 59 115 40 847.3 0 40 116 64 847.3 0 64 117 66 847.3 0 66 118 131 847.3 0 131 119 69 847.3 0 69 120 70 847.3 0 70 121 46 847.3 0 46 122 119 847.3 0 119 123 132 847.3 0 132 124 130 847.3 0 130 125 103 847.3 0 103 126 72 847.3 0 72 127 32 847.3 0 32 128 50 847.3 0 50 129 72 847.3 0 72 130 75 847.3 0 75 131 165 847.3 0 165 132 455 847.3 0 455 133 377 847.3 0 377 134 115 847.3 0 115 135 87 847.3 0 87 136 97 847.3 0 97 137 147 847.3 0 147 138 211 847.3 0 211 139 242 847.3 0 242 140 116 847.3 0 116 141 280 847.3 0 280 142 189 847.3 0 189 143 303 847.3 0 303 144 303 847.3 0 303 145 298 847.3 0 298 146 74 847.3 0 74 147 87 847.3 0 87 148 108 847.3 0 108 149 115 847.3 0 115 150 111 847.3 0 111 151 109 847.3 0 109 152 117 847.3 0 117 153 100 847.3 0 100 154 89 847.3 0 89 155 104 847.3 0 104 156 97 847.3 0 97 157 261 847.3 0 261 158 352 847.3 0 352 159 186 847.3 0 186 160 425 847.3 0 425 161 235 847.3 0 235 162 493 847.3 0 493 163 108 847.3 0 108 164 108 847.3 0 108 165 61 847.3 0 61 166 63 847.3 0 63 167 105 847.3 0 105 168 122 847.3 0 122 169 153 847.3 0 153 170 151 847.3 0 151 171 118 847.3 0 118 172 116 847.3 0 116 173 112 847.3 0 112 174 86 847.3 0 86 175 78 847.3 0 78 176 336 847.3 0 336 177 356 847.3 0 356 178 623 847.3 0 623 179 161 847.3 0 161 180 132 847.3 0 132 181 99 847.3 0 99 182 110 847.3 0 110 183 157 847.3 0 157 184 191 847.3 0 191 185 153 847.3 0 153 186 105 847.3 0 105 187 197 847.3 0 197 188 193 847.3 0 193 189 254 847.3 0 254 190 153 847.3 0 153 191 122 847.3 0 122 192 79 847.3 0 79 193 68 847.3 0 68 194 93 847.3 0 93 195 94 847.3 0 94 196 130 847.3 0 130 197 143 847.3 0 143 198 227 847.3 0 227 199 133 847.3 0 133 200 112 847.3 0 112 201 103 847.3 0 103 202 162 847.3 0 162 203 207 847.3 0 207 204 160 847.3 0 160 205 131 847.3 0 131 206 120 847.3 0 120 207 115 847.3 0 115 208 99 847.3 0 99 209 96 847.3 0 96 210 161 847.3 0 161 211 253 847.3 0 253 212 246 847.3 0 246 213 465 847.3 0 465 214 149 847.3 0 149 215 160 847.3 0 160 216 131 847.3 0 131 217 199 847.3 0 199 218 170 847.3 0 170 219 126 847.3 0 126 220 132 847.3 0 132 221 134 847.3 0 134 222 273 847.3 0 273 223 462 847.3 0 462 224 581 847.3 0 581 225 352 847.3 0 352 226 192 847.3 0 192 227 158 847.3 0 158 228 178 847.3 0 178 229 147 847.3 0 147 230 150 847.3 0 150 231 105 847.3 0 105 232 98 847.3 0 98 233 136 847.3 0 136 234 157 847.3 0 157 235 171 847.3 0 171 236 146 847.3 0 146 237 164 847.3 0 164 238 263 847.3 0 263 239 321 847.3 0 321 240 495 847.3 0 495 241 196 847.3 0 196 242 278 847.3 0 278 243 443 847.3 0 443 244 344 847.3 0 344 245 253 847.3 0 253 246 235 847.3 0 235 247 230 847.3 0 230 248 221 847.3 0 221 249 205 847.3 0 205 250 164 847.3 0 164 251 208 847.3 0 208 252 168 847.3 0 168 253 195 847.3 0 195 254 224 847.3 0 224 255 185 847.3 0 185 256 278 847.3 0 278 257 306 847.3 0 306 258 284 847.3 0 284 259 214 847.3 0 214 260 236 847.3 0 236 261 210 847.3 0 210 262 322 847.3 0 322 263 480 847.3 0 480 264 729 847.3 0 729 265 631 847.3 0 631 266 652 847.3 0 652 267 258 847.3 0 258 268 121 847.3 0 121 269 150 847.3 0 150 270 245 847.3 0 245 271 309 847.3 0 309 272 317 847.3 0 317 273 341 847.3 0 341 274 326 847.3 0 326 275 257 847.3 0 257 276 298 847.3 0 298 277 287 847.3 0 287 278 271 847.3 0 271 279 244 847.3 0 244 280 186 847.3 0 186 281 202 847.3 0 202 282 268 847.3 0 268 283 281 847.3 0 281 284 273 847.3 0 273 285 296 847.3 0 296 286 305 847.3 0 305 287 281 847.3 0 281 288 277 847.3 0 277 289 207 847.3 0 207 290 228 847.3 0 228 291 357 847.3 0 357 292 412 847.3 0 412 293 287 847.3 0 287 294 232 847.3 0 232 295 149 847.3 0 149 296 155 847.3 0 155 297 201 847.3 0 201 298 237 847.3 0 237 299 301 847.3 0 301 300 359 847.3 0 359 301 590 847.3 0 590 Finished in 1.34 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 24,436 Reads with adapters: 1,018 (4.2%) Reads that were too short: 91 (0.4%) Reads written (passing filters): 927 (3.8%) Total basepairs processed: 4,298,283 bp Total written (filtered): 212,815 bp (5.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1018 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 1 0.0 3 0 0 0 1 17 30 0.0 3 25 1 1 3 18 3 0.0 3 0 1 1 1 19 10 0.0 3 0 6 2 2 20 579 0.0 3 550 21 7 1 21 3 0.0 3 0 0 0 3 22 55 0.0 3 0 0 1 54 23 5 0.0 3 0 0 0 5 24 1 0.0 3 0 0 1 27 1 0.0 3 1 28 2 0.0 3 1 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 34 15 0.0 3 0 0 1 14 36 1 0.0 3 0 0 0 1 37 4 0.0 3 0 0 0 4 40 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 49 11 0.0 3 0 0 0 11 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 69 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 1 71 76 0.0 3 0 0 1 75 72 23 0.0 3 0 0 0 23 73 20 0.0 3 0 0 0 20 74 13 0.0 3 0 0 0 13 75 5 0.0 3 0 0 0 5 76 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 10 0.0 3 0 0 0 10 92 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 97 5 0.0 3 0 0 0 5 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 150 4 0.0 3 0 0 0 4 151 3 0.0 3 0 0 0 3 155 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 183 6 0.0 3 0 0 0 6 184 4 0.0 3 0 0 0 4 185 6 0.0 3 0 0 0 6 186 8 0.0 3 0 0 1 7 187 4 0.0 3 0 0 0 4 188 4 0.0 3 0 0 0 4 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 1 196 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (76 us/read; 0.79 M reads/minute). === Summary === Total reads processed: 927 Reads with adapters: 22 (2.4%) Reads written (passing filters): 927 (100.0%) Total basepairs processed: 212,815 bp Total written (filtered): 212,746 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 22 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.3% C: 4.5% G: 13.6% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 14.5 0 20 4 1 3.6 0 1 5 1 0.9 0 1