This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 164.77 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 29,854 (0.9%) Reads that were too short: 14,397 (0.4%) Reads written (passing filters): 15,457 (0.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 2,781,441 bp (0.3%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 29854 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 486 847.3 0 486 7 69 847.3 0 69 8 207 847.3 0 207 9 47 847.3 0 47 10 17 847.3 0 17 11 11 847.3 0 11 12 14 847.3 0 14 13 15 847.3 0 15 14 12 847.3 0 12 15 265 847.3 0 265 16 126 847.3 0 126 17 10 847.3 0 10 18 12 847.3 0 12 19 12 847.3 0 12 20 13 847.3 0 13 21 23 847.3 0 23 22 19 847.3 0 19 23 27 847.3 0 27 24 5 847.3 0 5 25 14 847.3 0 14 26 20 847.3 0 20 27 12 847.3 0 12 28 14 847.3 0 14 29 13 847.3 0 13 30 21 847.3 0 21 31 17 847.3 0 17 32 33 847.3 0 33 33 37 847.3 0 37 34 13 847.3 0 13 35 20 847.3 0 20 36 17 847.3 0 17 37 16 847.3 0 16 38 30 847.3 0 30 39 21 847.3 0 21 40 45 847.3 0 45 41 164 847.3 0 164 42 105 847.3 0 105 43 19 847.3 0 19 44 13 847.3 0 13 45 16 847.3 0 16 46 12 847.3 0 12 47 29 847.3 0 29 48 11 847.3 0 11 49 33 847.3 0 33 50 14 847.3 0 14 51 31 847.3 0 31 52 19 847.3 0 19 53 21 847.3 0 21 54 13 847.3 0 13 55 17 847.3 0 17 56 15 847.3 0 15 57 34 847.3 0 34 58 24 847.3 0 24 59 17 847.3 0 17 60 13 847.3 0 13 61 19 847.3 0 19 62 16 847.3 0 16 63 21 847.3 0 21 64 20 847.3 0 20 65 18 847.3 0 18 66 27 847.3 0 27 67 23 847.3 0 23 68 18 847.3 0 18 69 75 847.3 0 75 70 44 847.3 0 44 71 342 847.3 0 342 72 27 847.3 0 27 73 15 847.3 0 15 74 22 847.3 0 22 75 25 847.3 0 25 76 33 847.3 0 33 77 25 847.3 0 25 78 23 847.3 0 23 79 25 847.3 0 25 80 28 847.3 0 28 81 53 847.3 0 53 82 62 847.3 0 62 83 34 847.3 0 34 84 36 847.3 0 36 85 48 847.3 0 48 86 55 847.3 0 55 87 142 847.3 0 142 88 50 847.3 0 50 89 71 847.3 0 71 90 91 847.3 0 91 91 93 847.3 0 93 92 77 847.3 0 77 93 53 847.3 0 53 94 52 847.3 0 52 95 41 847.3 0 41 96 72 847.3 0 72 97 75 847.3 0 75 98 57 847.3 0 57 99 56 847.3 0 56 100 367 847.3 0 367 101 485 847.3 0 485 102 781 847.3 0 781 103 147 847.3 0 147 104 64 847.3 0 64 105 44 847.3 0 44 106 31 847.3 0 31 107 29 847.3 0 29 108 39 847.3 0 39 109 31 847.3 0 31 110 36 847.3 0 36 111 40 847.3 0 40 112 32 847.3 0 32 113 32 847.3 0 32 114 30 847.3 0 30 115 32 847.3 0 32 116 37 847.3 0 37 117 49 847.3 0 49 118 105 847.3 0 105 119 40 847.3 0 40 120 21 847.3 0 21 121 32 847.3 0 32 122 64 847.3 0 64 123 88 847.3 0 88 124 65 847.3 0 65 125 69 847.3 0 69 126 58 847.3 0 58 127 29 847.3 0 29 128 40 847.3 0 40 129 46 847.3 0 46 130 46 847.3 0 46 131 92 847.3 0 92 132 291 847.3 0 291 133 275 847.3 0 275 134 62 847.3 0 62 135 50 847.3 0 50 136 64 847.3 0 64 137 70 847.3 0 70 138 90 847.3 0 90 139 134 847.3 0 134 140 72 847.3 0 72 141 220 847.3 0 220 142 95 847.3 0 95 143 145 847.3 0 145 144 140 847.3 0 140 145 175 847.3 0 175 146 48 847.3 0 48 147 48 847.3 0 48 148 70 847.3 0 70 149 79 847.3 0 79 150 76 847.3 0 76 151 74 847.3 0 74 152 86 847.3 0 86 153 77 847.3 0 77 154 62 847.3 0 62 155 57 847.3 0 57 156 80 847.3 0 80 157 92 847.3 0 92 158 183 847.3 0 183 159 153 847.3 0 153 160 366 847.3 0 366 161 160 847.3 0 160 162 296 847.3 0 296 163 79 847.3 0 79 164 64 847.3 0 64 165 43 847.3 0 43 166 41 847.3 0 41 167 82 847.3 0 82 168 77 847.3 0 77 169 82 847.3 0 82 170 100 847.3 0 100 171 74 847.3 0 74 172 64 847.3 0 64 173 69 847.3 0 69 174 56 847.3 0 56 175 66 847.3 0 66 176 341 847.3 0 341 177 301 847.3 0 301 178 628 847.3 0 628 179 86 847.3 0 86 180 79 847.3 0 79 181 70 847.3 0 70 182 57 847.3 0 57 183 83 847.3 0 83 184 94 847.3 0 94 185 103 847.3 0 103 186 74 847.3 0 74 187 99 847.3 0 99 188 130 847.3 0 130 189 142 847.3 0 142 190 100 847.3 0 100 191 101 847.3 0 101 192 35 847.3 0 35 193 50 847.3 0 50 194 45 847.3 0 45 195 73 847.3 0 73 196 60 847.3 0 60 197 92 847.3 0 92 198 102 847.3 0 102 199 76 847.3 0 76 200 70 847.3 0 70 201 61 847.3 0 61 202 83 847.3 0 83 203 93 847.3 0 93 204 82 847.3 0 82 205 72 847.3 0 72 206 65 847.3 0 65 207 69 847.3 0 69 208 66 847.3 0 66 209 70 847.3 0 70 210 128 847.3 0 128 211 196 847.3 0 196 212 174 847.3 0 174 213 307 847.3 0 307 214 89 847.3 0 89 215 91 847.3 0 91 216 73 847.3 0 73 217 88 847.3 0 88 218 73 847.3 0 73 219 80 847.3 0 80 220 71 847.3 0 71 221 74 847.3 0 74 222 102 847.3 0 102 223 159 847.3 0 159 224 212 847.3 0 212 225 189 847.3 0 189 226 122 847.3 0 122 227 87 847.3 0 87 228 84 847.3 0 84 229 72 847.3 0 72 230 90 847.3 0 90 231 74 847.3 0 74 232 64 847.3 0 64 233 78 847.3 0 78 234 82 847.3 0 82 235 104 847.3 0 104 236 101 847.3 0 101 237 117 847.3 0 117 238 228 847.3 0 228 239 245 847.3 0 245 240 411 847.3 0 411 241 111 847.3 0 111 242 125 847.3 0 125 243 191 847.3 0 191 244 136 847.3 0 136 245 139 847.3 0 139 246 135 847.3 0 135 247 153 847.3 0 153 248 134 847.3 0 134 249 136 847.3 0 136 250 112 847.3 0 112 251 135 847.3 0 135 252 147 847.3 0 147 253 130 847.3 0 130 254 115 847.3 0 115 255 100 847.3 0 100 256 130 847.3 0 130 257 159 847.3 0 159 258 148 847.3 0 148 259 101 847.3 0 101 260 124 847.3 0 124 261 123 847.3 0 123 262 220 847.3 0 220 263 284 847.3 0 284 264 421 847.3 0 421 265 309 847.3 0 309 266 352 847.3 0 352 267 130 847.3 0 130 268 78 847.3 0 78 269 87 847.3 0 87 270 140 847.3 0 140 271 150 847.3 0 150 272 153 847.3 0 153 273 183 847.3 0 183 274 195 847.3 0 195 275 170 847.3 0 170 276 212 847.3 0 212 277 157 847.3 0 157 278 179 847.3 0 179 279 123 847.3 0 123 280 91 847.3 0 91 281 118 847.3 0 118 282 147 847.3 0 147 283 143 847.3 0 143 284 165 847.3 0 165 285 139 847.3 0 139 286 157 847.3 0 157 287 171 847.3 0 171 288 167 847.3 0 167 289 126 847.3 0 126 290 119 847.3 0 119 291 158 847.3 0 158 292 196 847.3 0 196 293 142 847.3 0 142 294 120 847.3 0 120 295 105 847.3 0 105 296 90 847.3 0 90 297 110 847.3 0 110 298 163 847.3 0 163 299 202 847.3 0 202 300 222 847.3 0 222 301 354 847.3 0 354 Finished in 0.93 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 15,457 Reads with adapters: 76 (0.5%) Reads that were too short: 14 (0.1%) Reads written (passing filters): 62 (0.4%) Total basepairs processed: 2,781,441 bp Total written (filtered): 16,334 bp (0.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 76 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 1 0.0 3 0 1 20 41 0.0 4 41 21 12 0.0 4 11 1 22 3 0.0 4 3 23 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (161 us/read; 0.37 M reads/minute). === Summary === Total reads processed: 62 Reads with adapters: 4 (6.5%) Reads written (passing filters): 62 (100.0%) Total basepairs processed: 16,334 bp Total written (filtered): 16,078 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 75.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 1 11 2 0.0 1 0 2 225 1 0.0 1 1