This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 165.81 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 87,335 (2.5%) Reads that were too short: 46,769 (1.3%) Reads written (passing filters): 40,566 (1.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 10,078,238 bp (1.0%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 87335 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 356 847.3 0 356 7 500 847.3 0 500 8 11899 847.3 0 11899 9 10672 847.3 0 10672 10 99 847.3 0 99 11 55 847.3 0 55 12 72 847.3 0 72 13 241 847.3 0 241 14 69 847.3 0 69 15 54 847.3 0 54 16 57 847.3 0 57 17 103 847.3 0 103 18 52 847.3 0 52 19 81 847.3 0 81 20 49 847.3 0 49 21 62 847.3 0 62 22 69 847.3 0 69 23 79 847.3 0 79 24 83 847.3 0 83 25 59 847.3 0 59 26 40 847.3 0 40 27 36 847.3 0 36 28 61 847.3 0 61 29 68 847.3 0 68 30 36 847.3 0 36 31 65 847.3 0 65 32 62 847.3 0 62 33 51 847.3 0 51 34 58 847.3 0 58 35 64 847.3 0 64 36 75 847.3 0 75 37 84 847.3 0 84 38 65 847.3 0 65 39 81 847.3 0 81 40 112 847.3 0 112 41 70 847.3 0 70 42 56 847.3 0 56 43 54 847.3 0 54 44 38 847.3 0 38 45 54 847.3 0 54 46 66 847.3 0 66 47 67 847.3 0 67 48 49 847.3 0 49 49 50 847.3 0 50 50 58 847.3 0 58 51 94 847.3 0 94 52 87 847.3 0 87 53 111 847.3 0 111 54 71 847.3 0 71 55 59 847.3 0 59 56 40 847.3 0 40 57 46 847.3 0 46 58 75 847.3 0 75 59 54 847.3 0 54 60 64 847.3 0 64 61 79 847.3 0 79 62 97 847.3 0 97 63 80 847.3 0 80 64 69 847.3 0 69 65 53 847.3 0 53 66 62 847.3 0 62 67 53 847.3 0 53 68 53 847.3 0 53 69 74 847.3 0 74 70 52 847.3 0 52 71 61 847.3 0 61 72 58 847.3 0 58 73 50 847.3 0 50 74 65 847.3 0 65 75 91 847.3 0 91 76 97 847.3 0 97 77 94 847.3 0 94 78 76 847.3 0 76 79 55 847.3 0 55 80 74 847.3 0 74 81 91 847.3 0 91 82 90 847.3 0 90 83 72 847.3 0 72 84 63 847.3 0 63 85 75 847.3 0 75 86 65 847.3 0 65 87 72 847.3 0 72 88 58 847.3 0 58 89 85 847.3 0 85 90 74 847.3 0 74 91 69 847.3 0 69 92 100 847.3 0 100 93 161 847.3 0 161 94 117 847.3 0 117 95 79 847.3 0 79 96 67 847.3 0 67 97 61 847.3 0 61 98 67 847.3 0 67 99 109 847.3 0 109 100 175 847.3 0 175 101 100 847.3 0 100 102 74 847.3 0 74 103 61 847.3 0 61 104 70 847.3 0 70 105 72 847.3 0 72 106 58 847.3 0 58 107 57 847.3 0 57 108 53 847.3 0 53 109 75 847.3 0 75 110 76 847.3 0 76 111 79 847.3 0 79 112 54 847.3 0 54 113 58 847.3 0 58 114 86 847.3 0 86 115 80 847.3 0 80 116 82 847.3 0 82 117 201 847.3 0 201 118 88 847.3 0 88 119 67 847.3 0 67 120 71 847.3 0 71 121 81 847.3 0 81 122 91 847.3 0 91 123 65 847.3 0 65 124 60 847.3 0 60 125 85 847.3 0 85 126 67 847.3 0 67 127 79 847.3 0 79 128 77 847.3 0 77 129 55 847.3 0 55 130 65 847.3 0 65 131 109 847.3 0 109 132 94 847.3 0 94 133 104 847.3 0 104 134 80 847.3 0 80 135 213 847.3 0 213 136 138 847.3 0 138 137 486 847.3 0 486 138 648 847.3 0 648 139 633 847.3 0 633 140 763 847.3 0 763 141 82 847.3 0 82 142 89 847.3 0 89 143 100 847.3 0 100 144 107 847.3 0 107 145 104 847.3 0 104 146 79 847.3 0 79 147 62 847.3 0 62 148 66 847.3 0 66 149 71 847.3 0 71 150 69 847.3 0 69 151 81 847.3 0 81 152 59 847.3 0 59 153 78 847.3 0 78 154 61 847.3 0 61 155 58 847.3 0 58 156 72 847.3 0 72 157 78 847.3 0 78 158 65 847.3 0 65 159 89 847.3 0 89 160 86 847.3 0 86 161 77 847.3 0 77 162 67 847.3 0 67 163 54 847.3 0 54 164 67 847.3 0 67 165 93 847.3 0 93 166 91 847.3 0 91 167 81 847.3 0 81 168 80 847.3 0 80 169 97 847.3 0 97 170 82 847.3 0 82 171 89 847.3 0 89 172 96 847.3 0 96 173 96 847.3 0 96 174 74 847.3 0 74 175 70 847.3 0 70 176 70 847.3 0 70 177 65 847.3 0 65 178 83 847.3 0 83 179 140 847.3 0 140 180 73 847.3 0 73 181 66 847.3 0 66 182 74 847.3 0 74 183 74 847.3 0 74 184 74 847.3 0 74 185 90 847.3 0 90 186 144 847.3 0 144 187 152 847.3 0 152 188 111 847.3 0 111 189 90 847.3 0 90 190 67 847.3 0 67 191 82 847.3 0 82 192 87 847.3 0 87 193 60 847.3 0 60 194 59 847.3 0 59 195 67 847.3 0 67 196 69 847.3 0 69 197 66 847.3 0 66 198 105 847.3 0 105 199 55 847.3 0 55 200 68 847.3 0 68 201 75 847.3 0 75 202 49 847.3 0 49 203 97 847.3 0 97 204 102 847.3 0 102 205 90 847.3 0 90 206 81 847.3 0 81 207 74 847.3 0 74 208 102 847.3 0 102 209 76 847.3 0 76 210 112 847.3 0 112 211 85 847.3 0 85 212 94 847.3 0 94 213 91 847.3 0 91 214 222 847.3 0 222 215 179 847.3 0 179 216 162 847.3 0 162 217 85 847.3 0 85 218 79 847.3 0 79 219 81 847.3 0 81 220 79 847.3 0 79 221 71 847.3 0 71 222 68 847.3 0 68 223 93 847.3 0 93 224 95 847.3 0 95 225 86 847.3 0 86 226 60 847.3 0 60 227 87 847.3 0 87 228 109 847.3 0 109 229 74 847.3 0 74 230 71 847.3 0 71 231 77 847.3 0 77 232 58 847.3 0 58 233 91 847.3 0 91 234 87 847.3 0 87 235 89 847.3 0 89 236 75 847.3 0 75 237 87 847.3 0 87 238 395 847.3 0 395 239 236 847.3 0 236 240 120 847.3 0 120 241 57 847.3 0 57 242 87 847.3 0 87 243 127 847.3 0 127 244 99 847.3 0 99 245 93 847.3 0 93 246 84 847.3 0 84 247 66 847.3 0 66 248 78 847.3 0 78 249 98 847.3 0 98 250 80 847.3 0 80 251 101 847.3 0 101 252 112 847.3 0 112 253 95 847.3 0 95 254 94 847.3 0 94 255 72 847.3 0 72 256 130 847.3 0 130 257 295 847.3 0 295 258 355 847.3 0 355 259 232 847.3 0 232 260 209 847.3 0 209 261 133 847.3 0 133 262 135 847.3 0 135 263 147 847.3 0 147 264 149 847.3 0 149 265 138 847.3 0 138 266 168 847.3 0 168 267 154 847.3 0 154 268 154 847.3 0 154 269 175 847.3 0 175 270 189 847.3 0 189 271 248 847.3 0 248 272 506 847.3 0 506 273 5899 847.3 0 5899 274 4491 847.3 0 4491 275 2523 847.3 0 2523 276 365 847.3 0 365 277 344 847.3 0 344 278 266 847.3 0 266 279 185 847.3 0 185 280 192 847.3 0 192 281 132 847.3 0 132 282 136 847.3 0 136 283 583 847.3 0 583 284 648 847.3 0 648 285 638 847.3 0 638 286 1088 847.3 0 1088 287 944 847.3 0 944 288 593 847.3 0 593 289 183 847.3 0 183 290 221 847.3 0 221 291 213 847.3 0 213 292 370 847.3 0 370 293 1191 847.3 0 1191 294 1378 847.3 0 1378 295 1571 847.3 0 1571 296 362 847.3 0 362 297 647 847.3 0 647 298 2007 847.3 0 2007 299 3334 847.3 0 3334 300 3750 847.3 0 3750 301 3586 847.3 0 3586 Finished in 2.84 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 40,566 Reads with adapters: 12,044 (29.7%) Reads that were too short: 61 (0.2%) Reads written (passing filters): 11,983 (29.5%) Total basepairs processed: 10,078,238 bp Total written (filtered): 3,296,173 bp (32.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12044 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 3 0.0 3 0 0 3 16 110 0.0 3 0 91 12 7 17 11679 0.0 3 11039 532 88 20 18 50 0.0 3 11 29 6 4 19 22 0.0 3 0 0 12 10 20 37 0.0 3 3 3 0 31 23 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 30 2 0.0 3 0 0 0 2 32 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 3 0.0 3 0 0 0 3 41 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 16 0.0 3 0 0 0 16 58 5 0.0 3 0 0 1 4 59 1 0.0 3 0 0 1 60 1 0.0 3 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 6 0.0 3 0 0 0 6 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 1 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 1 141 2 0.0 3 0 0 1 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 18 0.0 3 0 0 2 16 230 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 11,983 Reads with adapters: 54 (0.5%) Reads written (passing filters): 11,983 (100.0%) Total basepairs processed: 3,296,173 bp Total written (filtered): 3,296,002 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 54 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.4% C: 5.6% G: 9.3% T: 14.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 187.2 0 47 4 5 46.8 0 5 5 2 11.7 0 2