This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.20 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 151,468 (4.4%) Reads that were too short: 33,940 (1.0%) Reads written (passing filters): 117,528 (3.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 30,454,531 bp (2.9%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 151468 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5390 847.3 0 5390 7 42178 847.3 0 42178 8 35740 847.3 0 35740 9 63 847.3 0 63 10 29 847.3 0 29 11 33 847.3 0 33 12 37 847.3 0 37 13 35 847.3 0 35 14 36 847.3 0 36 15 41 847.3 0 41 16 32 847.3 0 32 17 29 847.3 0 29 18 22 847.3 0 22 19 23 847.3 0 23 20 37 847.3 0 37 21 31 847.3 0 31 22 45 847.3 0 45 23 26 847.3 0 26 24 32 847.3 0 32 25 36 847.3 0 36 26 25 847.3 0 25 27 49 847.3 0 49 28 34 847.3 0 34 29 20 847.3 0 20 30 36 847.3 0 36 31 64 847.3 0 64 32 34 847.3 0 34 33 39 847.3 0 39 34 50 847.3 0 50 35 39 847.3 0 39 36 54 847.3 0 54 37 73 847.3 0 73 38 42 847.3 0 42 39 51 847.3 0 51 40 62 847.3 0 62 41 55 847.3 0 55 42 50 847.3 0 50 43 58 847.3 0 58 44 55 847.3 0 55 45 56 847.3 0 56 46 54 847.3 0 54 47 73 847.3 0 73 48 122 847.3 0 122 49 133 847.3 0 133 50 1980 847.3 0 1980 51 2995 847.3 0 2995 52 987 847.3 0 987 53 28 847.3 0 28 54 27 847.3 0 27 55 25 847.3 0 25 56 28 847.3 0 28 57 42 847.3 0 42 58 37 847.3 0 37 59 53 847.3 0 53 60 58 847.3 0 58 61 70 847.3 0 70 62 51 847.3 0 51 63 71 847.3 0 71 64 80 847.3 0 80 65 85 847.3 0 85 66 57 847.3 0 57 67 77 847.3 0 77 68 52 847.3 0 52 69 40 847.3 0 40 70 42 847.3 0 42 71 51 847.3 0 51 72 74 847.3 0 74 73 121 847.3 0 121 74 604 847.3 0 604 75 543 847.3 0 543 76 890 847.3 0 890 77 105 847.3 0 105 78 246 847.3 0 246 79 70 847.3 0 70 80 91 847.3 0 91 81 39 847.3 0 39 82 61 847.3 0 61 83 36 847.3 0 36 84 50 847.3 0 50 85 62 847.3 0 62 86 33 847.3 0 33 87 38 847.3 0 38 88 32 847.3 0 32 89 43 847.3 0 43 90 47 847.3 0 47 91 115 847.3 0 115 92 93 847.3 0 93 93 72 847.3 0 72 94 63 847.3 0 63 95 35 847.3 0 35 96 38 847.3 0 38 97 35 847.3 0 35 98 66 847.3 0 66 99 44 847.3 0 44 100 34 847.3 0 34 101 44 847.3 0 44 102 40 847.3 0 40 103 41 847.3 0 41 104 46 847.3 0 46 105 35 847.3 0 35 106 46 847.3 0 46 107 31 847.3 0 31 108 32 847.3 0 32 109 44 847.3 0 44 110 28 847.3 0 28 111 49 847.3 0 49 112 63 847.3 0 63 113 63 847.3 0 63 114 99 847.3 0 99 115 134 847.3 0 134 116 78 847.3 0 78 117 111 847.3 0 111 118 111 847.3 0 111 119 108 847.3 0 108 120 114 847.3 0 114 121 80 847.3 0 80 122 83 847.3 0 83 123 120 847.3 0 120 124 141 847.3 0 141 125 1617 847.3 0 1617 126 1976 847.3 0 1976 127 621 847.3 0 621 128 98 847.3 0 98 129 123 847.3 0 123 130 362 847.3 0 362 131 209 847.3 0 209 132 204 847.3 0 204 133 50 847.3 0 50 134 77 847.3 0 77 135 59 847.3 0 59 136 228 847.3 0 228 137 73 847.3 0 73 138 68 847.3 0 68 139 42 847.3 0 42 140 47 847.3 0 47 141 46 847.3 0 46 142 66 847.3 0 66 143 58 847.3 0 58 144 72 847.3 0 72 145 51 847.3 0 51 146 68 847.3 0 68 147 34 847.3 0 34 148 58 847.3 0 58 149 55 847.3 0 55 150 35 847.3 0 35 151 49 847.3 0 49 152 51 847.3 0 51 153 44 847.3 0 44 154 55 847.3 0 55 155 52 847.3 0 52 156 38 847.3 0 38 157 56 847.3 0 56 158 48 847.3 0 48 159 54 847.3 0 54 160 62 847.3 0 62 161 36 847.3 0 36 162 54 847.3 0 54 163 59 847.3 0 59 164 380 847.3 0 380 165 334 847.3 0 334 166 342 847.3 0 342 167 75 847.3 0 75 168 89 847.3 0 89 169 57 847.3 0 57 170 66 847.3 0 66 171 81 847.3 0 81 172 62 847.3 0 62 173 39 847.3 0 39 174 56 847.3 0 56 175 50 847.3 0 50 176 48 847.3 0 48 177 66 847.3 0 66 178 50 847.3 0 50 179 88 847.3 0 88 180 91 847.3 0 91 181 138 847.3 0 138 182 96 847.3 0 96 183 122 847.3 0 122 184 111 847.3 0 111 185 66 847.3 0 66 186 101 847.3 0 101 187 94 847.3 0 94 188 80 847.3 0 80 189 145 847.3 0 145 190 184 847.3 0 184 191 516 847.3 0 516 192 378 847.3 0 378 193 205 847.3 0 205 194 230 847.3 0 230 195 276 847.3 0 276 196 1877 847.3 0 1877 197 2474 847.3 0 2474 198 2002 847.3 0 2002 199 1104 847.3 0 1104 200 477 847.3 0 477 201 143 847.3 0 143 202 187 847.3 0 187 203 212 847.3 0 212 204 1440 847.3 0 1440 205 824 847.3 0 824 206 2517 847.3 0 2517 207 113 847.3 0 113 208 129 847.3 0 129 209 121 847.3 0 121 210 106 847.3 0 106 211 146 847.3 0 146 212 99 847.3 0 99 213 111 847.3 0 111 214 108 847.3 0 108 215 172 847.3 0 172 216 1176 847.3 0 1176 217 1562 847.3 0 1562 218 759 847.3 0 759 219 251 847.3 0 251 220 149 847.3 0 149 221 151 847.3 0 151 222 120 847.3 0 120 223 117 847.3 0 117 224 92 847.3 0 92 225 80 847.3 0 80 226 130 847.3 0 130 227 97 847.3 0 97 228 82 847.3 0 82 229 78 847.3 0 78 230 73 847.3 0 73 231 78 847.3 0 78 232 77 847.3 0 77 233 62 847.3 0 62 234 105 847.3 0 105 235 230 847.3 0 230 236 91 847.3 0 91 237 73 847.3 0 73 238 139 847.3 0 139 239 143 847.3 0 143 240 199 847.3 0 199 241 257 847.3 0 257 242 107 847.3 0 107 243 106 847.3 0 106 244 100 847.3 0 100 245 144 847.3 0 144 246 169 847.3 0 169 247 1262 847.3 0 1262 248 1644 847.3 0 1644 249 1410 847.3 0 1410 250 852 847.3 0 852 251 242 847.3 0 242 252 120 847.3 0 120 253 97 847.3 0 97 254 90 847.3 0 90 255 82 847.3 0 82 256 88 847.3 0 88 257 111 847.3 0 111 258 120 847.3 0 120 259 94 847.3 0 94 260 220 847.3 0 220 261 127 847.3 0 127 262 201 847.3 0 201 263 141 847.3 0 141 264 129 847.3 0 129 265 110 847.3 0 110 266 83 847.3 0 83 267 73 847.3 0 73 268 98 847.3 0 98 269 421 847.3 0 421 270 369 847.3 0 369 271 551 847.3 0 551 272 99 847.3 0 99 273 90 847.3 0 90 274 69 847.3 0 69 275 75 847.3 0 75 276 107 847.3 0 107 277 160 847.3 0 160 278 152 847.3 0 152 279 269 847.3 0 269 280 186 847.3 0 186 281 96 847.3 0 96 282 86 847.3 0 86 283 155 847.3 0 155 284 125 847.3 0 125 285 123 847.3 0 123 286 185 847.3 0 185 287 157 847.3 0 157 288 166 847.3 0 166 289 962 847.3 0 962 290 1995 847.3 0 1995 291 2636 847.3 0 2636 292 1580 847.3 0 1580 293 417 847.3 0 417 294 135 847.3 0 135 295 121 847.3 0 121 296 122 847.3 0 122 297 284 847.3 0 284 298 363 847.3 0 363 299 478 847.3 0 478 300 335 847.3 0 335 301 195 847.3 0 195 Finished in 8.54 s (73 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 117,528 Reads with adapters: 43,244 (36.8%) Reads that were too short: 185 (0.2%) Reads written (passing filters): 43,059 (36.6%) Total basepairs processed: 30,454,531 bp Total written (filtered): 11,884,887 bp (39.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43244 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 20 0.0 3 0 0 11 9 16 363 0.0 3 0 312 34 17 17 42008 0.0 3 39614 2003 327 64 18 184 0.0 3 14 52 8 110 19 91 0.0 3 0 0 55 36 20 106 0.0 3 8 4 0 94 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 23 0.0 3 0 0 0 23 28 2 0.0 3 0 0 1 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 1 35 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 93 0.0 3 0 0 1 92 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 17 0.0 3 0 0 1 16 53 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 12 0.0 3 0 0 0 12 64 1 0.0 3 0 0 0 1 65 5 0.0 3 0 0 0 5 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 9 0.0 3 0 0 0 9 76 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 24 0.0 3 0 0 0 24 88 4 0.0 3 1 0 0 3 89 4 0.0 3 0 0 1 3 90 15 0.0 3 0 0 2 13 91 3 0.0 3 0 0 0 3 92 4 0.0 3 0 0 0 4 94 2 0.0 3 0 0 0 2 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 9 0.0 3 0 0 0 9 99 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 1 1 104 5 0.0 3 0 0 0 5 105 7 0.0 3 0 0 1 6 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 5 0.0 3 0 0 0 5 114 2 0.0 3 0 0 0 2 115 4 0.0 3 0 0 0 4 116 3 0.0 3 0 0 0 3 117 29 0.0 3 0 0 3 26 118 3 0.0 3 0 0 0 3 120 6 0.0 3 0 0 0 6 121 7 0.0 3 0 0 0 7 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 141 9 0.0 3 0 0 0 9 142 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 148 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 5 0.0 3 0 0 0 5 160 3 0.0 3 0 0 1 2 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 176 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 6 0.0 3 0 0 1 5 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 242 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 1 253 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 5 0.0 3 0 0 0 5 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.27 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 43,059 Reads with adapters: 235 (0.5%) Reads written (passing filters): 43,059 (100.0%) Total basepairs processed: 11,884,887 bp Total written (filtered): 11,884,146 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 235 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.5% C: 46.8% G: 22.6% T: 5.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 208 672.8 0 208 4 20 168.2 0 20 5 5 42.0 0 5 6 2 10.5 0 2