This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 165.70 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 168,801 (4.9%) Reads that were too short: 127,479 (3.7%) Reads written (passing filters): 41,322 (1.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 7,019,933 bp (0.7%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 168801 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 344 847.3 0 344 7 167 847.3 0 167 8 36 847.3 0 36 9 128 847.3 0 128 10 62 847.3 0 62 11 48 847.3 0 48 12 49 847.3 0 49 13 33 847.3 0 33 14 48 847.3 0 48 15 106 847.3 0 106 16 74 847.3 0 74 17 60 847.3 0 60 18 69 847.3 0 69 19 61 847.3 0 61 20 76 847.3 0 76 21 29 847.3 0 29 22 51 847.3 0 51 23 63 847.3 0 63 24 43 847.3 0 43 25 50 847.3 0 50 26 53 847.3 0 53 27 50 847.3 0 50 28 60 847.3 0 60 29 43 847.3 0 43 30 62 847.3 0 62 31 35 847.3 0 35 32 53 847.3 0 53 33 42 847.3 0 42 34 43 847.3 0 43 35 66 847.3 0 66 36 56 847.3 0 56 37 54 847.3 0 54 38 78 847.3 0 78 39 60 847.3 0 60 40 93 847.3 0 93 41 90 847.3 0 90 42 47 847.3 0 47 43 48 847.3 0 48 44 58 847.3 0 58 45 47 847.3 0 47 46 43 847.3 0 43 47 60 847.3 0 60 48 66 847.3 0 66 49 56 847.3 0 56 50 64 847.3 0 64 51 91 847.3 0 91 52 56 847.3 0 56 53 73 847.3 0 73 54 56 847.3 0 56 55 65 847.3 0 65 56 94 847.3 0 94 57 392 847.3 0 392 58 524 847.3 0 524 59 1598 847.3 0 1598 60 49 847.3 0 49 61 54 847.3 0 54 62 40 847.3 0 40 63 40 847.3 0 40 64 60 847.3 0 60 65 87 847.3 0 87 66 37 847.3 0 37 67 30 847.3 0 30 68 49 847.3 0 49 69 64 847.3 0 64 70 46 847.3 0 46 71 53 847.3 0 53 72 65 847.3 0 65 73 61 847.3 0 61 74 120 847.3 0 120 75 67 847.3 0 67 76 64 847.3 0 64 77 47 847.3 0 47 78 53 847.3 0 53 79 91 847.3 0 91 80 94 847.3 0 94 81 62 847.3 0 62 82 63 847.3 0 63 83 96 847.3 0 96 84 73 847.3 0 73 85 47 847.3 0 47 86 59 847.3 0 59 87 73 847.3 0 73 88 107 847.3 0 107 89 114 847.3 0 114 90 123 847.3 0 123 91 111 847.3 0 111 92 128 847.3 0 128 93 67 847.3 0 67 94 52 847.3 0 52 95 77 847.3 0 77 96 66 847.3 0 66 97 56 847.3 0 56 98 117 847.3 0 117 99 113 847.3 0 113 100 63 847.3 0 63 101 55 847.3 0 55 102 73 847.3 0 73 103 80 847.3 0 80 104 134 847.3 0 134 105 187 847.3 0 187 106 154 847.3 0 154 107 289 847.3 0 289 108 167 847.3 0 167 109 143 847.3 0 143 110 117 847.3 0 117 111 78 847.3 0 78 112 69 847.3 0 69 113 66 847.3 0 66 114 87 847.3 0 87 115 98 847.3 0 98 116 109 847.3 0 109 117 140 847.3 0 140 118 92 847.3 0 92 119 117 847.3 0 117 120 184 847.3 0 184 121 251 847.3 0 251 122 1325 847.3 0 1325 123 759 847.3 0 759 124 1499 847.3 0 1499 125 88 847.3 0 88 126 75 847.3 0 75 127 102 847.3 0 102 128 84 847.3 0 84 129 108 847.3 0 108 130 136 847.3 0 136 131 127 847.3 0 127 132 120 847.3 0 120 133 136 847.3 0 136 134 126 847.3 0 126 135 168 847.3 0 168 136 116 847.3 0 116 137 177 847.3 0 177 138 96 847.3 0 96 139 152 847.3 0 152 140 145 847.3 0 145 141 359 847.3 0 359 142 471 847.3 0 471 143 570 847.3 0 570 144 170 847.3 0 170 145 128 847.3 0 128 146 149 847.3 0 149 147 264 847.3 0 264 148 411 847.3 0 411 149 581 847.3 0 581 150 367 847.3 0 367 151 2379 847.3 0 2379 152 2448 847.3 0 2448 153 5317 847.3 0 5317 154 135 847.3 0 135 155 191 847.3 0 191 156 180 847.3 0 180 157 429 847.3 0 429 158 394 847.3 0 394 159 540 847.3 0 540 160 169 847.3 0 169 161 241 847.3 0 241 162 352 847.3 0 352 163 417 847.3 0 417 164 209 847.3 0 209 165 101 847.3 0 101 166 101 847.3 0 101 167 141 847.3 0 141 168 151 847.3 0 151 169 162 847.3 0 162 170 145 847.3 0 145 171 128 847.3 0 128 172 73 847.3 0 73 173 71 847.3 0 71 174 104 847.3 0 104 175 103 847.3 0 103 176 229 847.3 0 229 177 253 847.3 0 253 178 150 847.3 0 150 179 144 847.3 0 144 180 106 847.3 0 106 181 133 847.3 0 133 182 413 847.3 0 413 183 326 847.3 0 326 184 276 847.3 0 276 185 153 847.3 0 153 186 306 847.3 0 306 187 228 847.3 0 228 188 225 847.3 0 225 189 253 847.3 0 253 190 90 847.3 0 90 191 86 847.3 0 86 192 88 847.3 0 88 193 134 847.3 0 134 194 170 847.3 0 170 195 232 847.3 0 232 196 501 847.3 0 501 197 263 847.3 0 263 198 303 847.3 0 303 199 127 847.3 0 127 200 241 847.3 0 241 201 601 847.3 0 601 202 408 847.3 0 408 203 349 847.3 0 349 204 191 847.3 0 191 205 238 847.3 0 238 206 354 847.3 0 354 207 373 847.3 0 373 208 704 847.3 0 704 209 1462 847.3 0 1462 210 1765 847.3 0 1765 211 2529 847.3 0 2529 212 2523 847.3 0 2523 213 18690 847.3 0 18690 214 19459 847.3 0 19459 215 46974 847.3 0 46974 216 1635 847.3 0 1635 217 203 847.3 0 203 218 196 847.3 0 196 219 210 847.3 0 210 220 338 847.3 0 338 221 1475 847.3 0 1475 222 2571 847.3 0 2571 223 4737 847.3 0 4737 224 710 847.3 0 710 225 116 847.3 0 116 226 128 847.3 0 128 227 151 847.3 0 151 228 109 847.3 0 109 229 96 847.3 0 96 230 101 847.3 0 101 231 211 847.3 0 211 232 228 847.3 0 228 233 163 847.3 0 163 234 112 847.3 0 112 235 153 847.3 0 153 236 164 847.3 0 164 237 123 847.3 0 123 238 125 847.3 0 125 239 121 847.3 0 121 240 113 847.3 0 113 241 141 847.3 0 141 242 159 847.3 0 159 243 209 847.3 0 209 244 277 847.3 0 277 245 371 847.3 0 371 246 341 847.3 0 341 247 112 847.3 0 112 248 100 847.3 0 100 249 108 847.3 0 108 250 114 847.3 0 114 251 145 847.3 0 145 252 130 847.3 0 130 253 122 847.3 0 122 254 144 847.3 0 144 255 214 847.3 0 214 256 208 847.3 0 208 257 516 847.3 0 516 258 560 847.3 0 560 259 912 847.3 0 912 260 606 847.3 0 606 261 459 847.3 0 459 262 397 847.3 0 397 263 729 847.3 0 729 264 624 847.3 0 624 265 376 847.3 0 376 266 135 847.3 0 135 267 125 847.3 0 125 268 168 847.3 0 168 269 290 847.3 0 290 270 435 847.3 0 435 271 360 847.3 0 360 272 327 847.3 0 327 273 152 847.3 0 152 274 175 847.3 0 175 275 185 847.3 0 185 276 244 847.3 0 244 277 233 847.3 0 233 278 191 847.3 0 191 279 155 847.3 0 155 280 170 847.3 0 170 281 374 847.3 0 374 282 487 847.3 0 487 283 489 847.3 0 489 284 588 847.3 0 588 285 531 847.3 0 531 286 427 847.3 0 427 287 203 847.3 0 203 288 151 847.3 0 151 289 232 847.3 0 232 290 310 847.3 0 310 291 185 847.3 0 185 292 143 847.3 0 143 293 116 847.3 0 116 294 183 847.3 0 183 295 211 847.3 0 211 296 258 847.3 0 258 297 259 847.3 0 259 298 171 847.3 0 171 299 163 847.3 0 163 300 159 847.3 0 159 301 112 847.3 0 112 Finished in 2.27 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 41,322 Reads with adapters: 253 (0.6%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 203 (0.5%) Total basepairs processed: 7,019,933 bp Total written (filtered): 37,611 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 253 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 7 0.0 3 0 4 0 3 17 66 0.0 3 55 6 4 1 18 12 0.0 3 0 0 0 12 19 8 0.0 3 4 1 3 20 1 0.0 3 0 0 0 1 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 1 32 7 0.0 3 0 0 0 7 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 1 50 8 0.0 3 0 0 0 8 51 3 0.0 3 0 0 1 2 53 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 1 124 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 62 0.0 3 0 0 0 62 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 1 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 203 Reads with adapters: 6 (3.0%) Reads written (passing filters): 203 (100.0%) Total basepairs processed: 37,611 bp Total written (filtered): 37,593 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.7% C: 33.3% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 3.2 0 6