This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 164.09 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 161,132 (4.6%) Reads that were too short: 122,353 (3.5%) Reads written (passing filters): 38,779 (1.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 6,734,796 bp (0.6%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 161132 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 424 847.3 0 424 7 175 847.3 0 175 8 27 847.3 0 27 9 125 847.3 0 125 10 39 847.3 0 39 11 30 847.3 0 30 12 33 847.3 0 33 13 30 847.3 0 30 14 25 847.3 0 25 15 47 847.3 0 47 16 53 847.3 0 53 17 71 847.3 0 71 18 51 847.3 0 51 19 35 847.3 0 35 20 39 847.3 0 39 21 26 847.3 0 26 22 48 847.3 0 48 23 56 847.3 0 56 24 38 847.3 0 38 25 41 847.3 0 41 26 20 847.3 0 20 27 476 847.3 0 476 28 318 847.3 0 318 29 39 847.3 0 39 30 43 847.3 0 43 31 31 847.3 0 31 32 38 847.3 0 38 33 63 847.3 0 63 34 42 847.3 0 42 35 38 847.3 0 38 36 36 847.3 0 36 37 35 847.3 0 35 38 46 847.3 0 46 39 34 847.3 0 34 40 81 847.3 0 81 41 80 847.3 0 80 42 49 847.3 0 49 43 43 847.3 0 43 44 34 847.3 0 34 45 39 847.3 0 39 46 49 847.3 0 49 47 39 847.3 0 39 48 49 847.3 0 49 49 49 847.3 0 49 50 57 847.3 0 57 51 63 847.3 0 63 52 55 847.3 0 55 53 53 847.3 0 53 54 61 847.3 0 61 55 74 847.3 0 74 56 99 847.3 0 99 57 679 847.3 0 679 58 740 847.3 0 740 59 2070 847.3 0 2070 60 47 847.3 0 47 61 38 847.3 0 38 62 40 847.3 0 40 63 45 847.3 0 45 64 42 847.3 0 42 65 43 847.3 0 43 66 30 847.3 0 30 67 46 847.3 0 46 68 57 847.3 0 57 69 44 847.3 0 44 70 27 847.3 0 27 71 36 847.3 0 36 72 50 847.3 0 50 73 38 847.3 0 38 74 49 847.3 0 49 75 32 847.3 0 32 76 44 847.3 0 44 77 55 847.3 0 55 78 58 847.3 0 58 79 65 847.3 0 65 80 95 847.3 0 95 81 56 847.3 0 56 82 65 847.3 0 65 83 94 847.3 0 94 84 114 847.3 0 114 85 72 847.3 0 72 86 101 847.3 0 101 87 91 847.3 0 91 88 204 847.3 0 204 89 164 847.3 0 164 90 284 847.3 0 284 91 148 847.3 0 148 92 134 847.3 0 134 93 83 847.3 0 83 94 57 847.3 0 57 95 60 847.3 0 60 96 59 847.3 0 59 97 60 847.3 0 60 98 96 847.3 0 96 99 81 847.3 0 81 100 76 847.3 0 76 101 67 847.3 0 67 102 71 847.3 0 71 103 73 847.3 0 73 104 110 847.3 0 110 105 151 847.3 0 151 106 134 847.3 0 134 107 169 847.3 0 169 108 122 847.3 0 122 109 95 847.3 0 95 110 92 847.3 0 92 111 68 847.3 0 68 112 59 847.3 0 59 113 71 847.3 0 71 114 77 847.3 0 77 115 93 847.3 0 93 116 77 847.3 0 77 117 130 847.3 0 130 118 67 847.3 0 67 119 79 847.3 0 79 120 108 847.3 0 108 121 150 847.3 0 150 122 710 847.3 0 710 123 548 847.3 0 548 124 969 847.3 0 969 125 65 847.3 0 65 126 75 847.3 0 75 127 84 847.3 0 84 128 88 847.3 0 88 129 75 847.3 0 75 130 106 847.3 0 106 131 90 847.3 0 90 132 99 847.3 0 99 133 98 847.3 0 98 134 124 847.3 0 124 135 178 847.3 0 178 136 118 847.3 0 118 137 160 847.3 0 160 138 84 847.3 0 84 139 130 847.3 0 130 140 118 847.3 0 118 141 256 847.3 0 256 142 317 847.3 0 317 143 423 847.3 0 423 144 108 847.3 0 108 145 108 847.3 0 108 146 127 847.3 0 127 147 203 847.3 0 203 148 331 847.3 0 331 149 435 847.3 0 435 150 264 847.3 0 264 151 2831 847.3 0 2831 152 2732 847.3 0 2732 153 5705 847.3 0 5705 154 126 847.3 0 126 155 173 847.3 0 173 156 137 847.3 0 137 157 436 847.3 0 436 158 390 847.3 0 390 159 650 847.3 0 650 160 120 847.3 0 120 161 158 847.3 0 158 162 238 847.3 0 238 163 229 847.3 0 229 164 106 847.3 0 106 165 70 847.3 0 70 166 80 847.3 0 80 167 73 847.3 0 73 168 123 847.3 0 123 169 105 847.3 0 105 170 141 847.3 0 141 171 119 847.3 0 119 172 63 847.3 0 63 173 59 847.3 0 59 174 80 847.3 0 80 175 92 847.3 0 92 176 182 847.3 0 182 177 191 847.3 0 191 178 113 847.3 0 113 179 125 847.3 0 125 180 81 847.3 0 81 181 120 847.3 0 120 182 287 847.3 0 287 183 200 847.3 0 200 184 207 847.3 0 207 185 144 847.3 0 144 186 320 847.3 0 320 187 179 847.3 0 179 188 165 847.3 0 165 189 180 847.3 0 180 190 83 847.3 0 83 191 63 847.3 0 63 192 72 847.3 0 72 193 141 847.3 0 141 194 140 847.3 0 140 195 243 847.3 0 243 196 456 847.3 0 456 197 159 847.3 0 159 198 200 847.3 0 200 199 108 847.3 0 108 200 226 847.3 0 226 201 589 847.3 0 589 202 448 847.3 0 448 203 329 847.3 0 329 204 203 847.3 0 203 205 208 847.3 0 208 206 372 847.3 0 372 207 319 847.3 0 319 208 477 847.3 0 477 209 1016 847.3 0 1016 210 1082 847.3 0 1082 211 1812 847.3 0 1812 212 1903 847.3 0 1903 213 20850 847.3 0 20850 214 20500 847.3 0 20500 215 52256 847.3 0 52256 216 2174 847.3 0 2174 217 125 847.3 0 125 218 119 847.3 0 119 219 145 847.3 0 145 220 211 847.3 0 211 221 887 847.3 0 887 222 1330 847.3 0 1330 223 2459 847.3 0 2459 224 354 847.3 0 354 225 90 847.3 0 90 226 93 847.3 0 93 227 121 847.3 0 121 228 84 847.3 0 84 229 76 847.3 0 76 230 95 847.3 0 95 231 166 847.3 0 166 232 164 847.3 0 164 233 109 847.3 0 109 234 109 847.3 0 109 235 178 847.3 0 178 236 127 847.3 0 127 237 95 847.3 0 95 238 71 847.3 0 71 239 84 847.3 0 84 240 100 847.3 0 100 241 106 847.3 0 106 242 118 847.3 0 118 243 149 847.3 0 149 244 170 847.3 0 170 245 209 847.3 0 209 246 177 847.3 0 177 247 117 847.3 0 117 248 85 847.3 0 85 249 114 847.3 0 114 250 111 847.3 0 111 251 107 847.3 0 107 252 113 847.3 0 113 253 86 847.3 0 86 254 100 847.3 0 100 255 124 847.3 0 124 256 156 847.3 0 156 257 290 847.3 0 290 258 348 847.3 0 348 259 499 847.3 0 499 260 446 847.3 0 446 261 222 847.3 0 222 262 207 847.3 0 207 263 400 847.3 0 400 264 340 847.3 0 340 265 272 847.3 0 272 266 100 847.3 0 100 267 77 847.3 0 77 268 132 847.3 0 132 269 206 847.3 0 206 270 223 847.3 0 223 271 191 847.3 0 191 272 180 847.3 0 180 273 108 847.3 0 108 274 146 847.3 0 146 275 118 847.3 0 118 276 183 847.3 0 183 277 194 847.3 0 194 278 132 847.3 0 132 279 115 847.3 0 115 280 130 847.3 0 130 281 315 847.3 0 315 282 329 847.3 0 329 283 302 847.3 0 302 284 281 847.3 0 281 285 211 847.3 0 211 286 216 847.3 0 216 287 128 847.3 0 128 288 109 847.3 0 109 289 147 847.3 0 147 290 174 847.3 0 174 291 139 847.3 0 139 292 108 847.3 0 108 293 99 847.3 0 99 294 137 847.3 0 137 295 184 847.3 0 184 296 180 847.3 0 180 297 156 847.3 0 156 298 138 847.3 0 138 299 155 847.3 0 155 300 113 847.3 0 113 301 90 847.3 0 90 Finished in 2.31 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 38,779 Reads with adapters: 526 (1.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 517 (1.3%) Total basepairs processed: 6,734,796 bp Total written (filtered): 138,615 bp (2.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 526 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 2 0.0 3 0 2 20 109 0.0 4 107 2 22 25 0.0 4 18 7 23 363 0.0 4 356 7 27 13 0.0 4 0 0 0 0 13 35 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 1 72 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 219 1 0.0 4 1 248 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 517 Reads with adapters: 63 (12.2%) Reads written (passing filters): 517 (100.0%) Total basepairs processed: 138,615 bp Total written (filtered): 137,802 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 63 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 3.2% G: 92.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 38 0.0 0 4 34 10 23 0.0 1 0 23 11 1 0.0 1 0 1 230 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.