This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.51 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 508,099 (14.6%) Reads that were too short: 19,045 (0.5%) Reads written (passing filters): 489,054 (14.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 114,788,664 bp (11.0%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 508099 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1870 847.3 0 1870 7 3341 847.3 0 3341 8 66549 847.3 0 66549 9 54231 847.3 0 54231 10 138 847.3 0 138 11 77 847.3 0 77 12 108 847.3 0 108 13 87 847.3 0 87 14 71 847.3 0 71 15 96 847.3 0 96 16 70 847.3 0 70 17 96 847.3 0 96 18 114 847.3 0 114 19 118 847.3 0 118 20 130 847.3 0 130 21 110 847.3 0 110 22 162 847.3 0 162 23 294 847.3 0 294 24 172 847.3 0 172 25 106 847.3 0 106 26 108 847.3 0 108 27 110 847.3 0 110 28 88 847.3 0 88 29 133 847.3 0 133 30 131 847.3 0 131 31 117 847.3 0 117 32 117 847.3 0 117 33 118 847.3 0 118 34 139 847.3 0 139 35 111 847.3 0 111 36 194 847.3 0 194 37 190 847.3 0 190 38 345 847.3 0 345 39 197 847.3 0 197 40 193 847.3 0 193 41 133 847.3 0 133 42 321 847.3 0 321 43 174 847.3 0 174 44 232 847.3 0 232 45 347 847.3 0 347 46 480 847.3 0 480 47 647 847.3 0 647 48 1295 847.3 0 1295 49 2331 847.3 0 2331 50 623 847.3 0 623 51 718 847.3 0 718 52 1142 847.3 0 1142 53 853 847.3 0 853 54 754 847.3 0 754 55 1285 847.3 0 1285 56 1067 847.3 0 1067 57 1448 847.3 0 1448 58 1451 847.3 0 1451 59 2686 847.3 0 2686 60 3121 847.3 0 3121 61 6500 847.3 0 6500 62 124015 847.3 0 124015 63 67549 847.3 0 67549 64 37891 847.3 0 37891 65 745 847.3 0 745 66 110 847.3 0 110 67 99 847.3 0 99 68 88 847.3 0 88 69 110 847.3 0 110 70 127 847.3 0 127 71 218 847.3 0 218 72 356 847.3 0 356 73 555 847.3 0 555 74 233 847.3 0 233 75 183 847.3 0 183 76 938 847.3 0 938 77 2246 847.3 0 2246 78 2636 847.3 0 2636 79 241 847.3 0 241 80 161 847.3 0 161 81 84 847.3 0 84 82 120 847.3 0 120 83 96 847.3 0 96 84 76 847.3 0 76 85 105 847.3 0 105 86 77 847.3 0 77 87 77 847.3 0 77 88 85 847.3 0 85 89 73 847.3 0 73 90 108 847.3 0 108 91 127 847.3 0 127 92 89 847.3 0 89 93 134 847.3 0 134 94 128 847.3 0 128 95 130 847.3 0 130 96 56 847.3 0 56 97 72 847.3 0 72 98 279 847.3 0 279 99 191 847.3 0 191 100 304 847.3 0 304 101 91 847.3 0 91 102 88 847.3 0 88 103 134 847.3 0 134 104 136 847.3 0 136 105 110 847.3 0 110 106 117 847.3 0 117 107 336 847.3 0 336 108 718 847.3 0 718 109 873 847.3 0 873 110 264 847.3 0 264 111 199 847.3 0 199 112 206 847.3 0 206 113 75 847.3 0 75 114 75 847.3 0 75 115 105 847.3 0 105 116 92 847.3 0 92 117 115 847.3 0 115 118 88 847.3 0 88 119 71 847.3 0 71 120 93 847.3 0 93 121 90 847.3 0 90 122 61 847.3 0 61 123 79 847.3 0 79 124 92 847.3 0 92 125 104 847.3 0 104 126 154 847.3 0 154 127 188 847.3 0 188 128 144 847.3 0 144 129 164 847.3 0 164 130 131 847.3 0 131 131 129 847.3 0 129 132 159 847.3 0 159 133 152 847.3 0 152 134 153 847.3 0 153 135 115 847.3 0 115 136 109 847.3 0 109 137 204 847.3 0 204 138 140 847.3 0 140 139 208 847.3 0 208 140 224 847.3 0 224 141 296 847.3 0 296 142 468 847.3 0 468 143 597 847.3 0 597 144 4413 847.3 0 4413 145 4396 847.3 0 4396 146 9103 847.3 0 9103 147 363 847.3 0 363 148 300 847.3 0 300 149 207 847.3 0 207 150 178 847.3 0 178 151 192 847.3 0 192 152 340 847.3 0 340 153 264 847.3 0 264 154 286 847.3 0 286 155 258 847.3 0 258 156 1361 847.3 0 1361 157 1275 847.3 0 1275 158 2871 847.3 0 2871 159 356 847.3 0 356 160 363 847.3 0 363 161 373 847.3 0 373 162 631 847.3 0 631 163 907 847.3 0 907 164 7098 847.3 0 7098 165 8692 847.3 0 8692 166 15762 847.3 0 15762 167 261 847.3 0 261 168 260 847.3 0 260 169 953 847.3 0 953 170 2648 847.3 0 2648 171 3186 847.3 0 3186 172 1956 847.3 0 1956 173 915 847.3 0 915 174 1505 847.3 0 1505 175 279 847.3 0 279 176 87 847.3 0 87 177 118 847.3 0 118 178 134 847.3 0 134 179 230 847.3 0 230 180 489 847.3 0 489 181 261 847.3 0 261 182 123 847.3 0 123 183 182 847.3 0 182 184 274 847.3 0 274 185 259 847.3 0 259 186 224 847.3 0 224 187 230 847.3 0 230 188 239 847.3 0 239 189 1684 847.3 0 1684 190 2091 847.3 0 2091 191 1585 847.3 0 1585 192 928 847.3 0 928 193 88 847.3 0 88 194 94 847.3 0 94 195 70 847.3 0 70 196 104 847.3 0 104 197 156 847.3 0 156 198 186 847.3 0 186 199 162 847.3 0 162 200 112 847.3 0 112 201 94 847.3 0 94 202 104 847.3 0 104 203 112 847.3 0 112 204 112 847.3 0 112 205 102 847.3 0 102 206 141 847.3 0 141 207 112 847.3 0 112 208 148 847.3 0 148 209 98 847.3 0 98 210 85 847.3 0 85 211 87 847.3 0 87 212 103 847.3 0 103 213 110 847.3 0 110 214 139 847.3 0 139 215 213 847.3 0 213 216 266 847.3 0 266 217 381 847.3 0 381 218 282 847.3 0 282 219 157 847.3 0 157 220 76 847.3 0 76 221 67 847.3 0 67 222 77 847.3 0 77 223 82 847.3 0 82 224 127 847.3 0 127 225 146 847.3 0 146 226 354 847.3 0 354 227 378 847.3 0 378 228 375 847.3 0 375 229 286 847.3 0 286 230 183 847.3 0 183 231 434 847.3 0 434 232 111 847.3 0 111 233 87 847.3 0 87 234 89 847.3 0 89 235 110 847.3 0 110 236 113 847.3 0 113 237 138 847.3 0 138 238 187 847.3 0 187 239 139 847.3 0 139 240 133 847.3 0 133 241 94 847.3 0 94 242 70 847.3 0 70 243 156 847.3 0 156 244 163 847.3 0 163 245 150 847.3 0 150 246 146 847.3 0 146 247 201 847.3 0 201 248 192 847.3 0 192 249 150 847.3 0 150 250 103 847.3 0 103 251 112 847.3 0 112 252 124 847.3 0 124 253 118 847.3 0 118 254 111 847.3 0 111 255 82 847.3 0 82 256 96 847.3 0 96 257 107 847.3 0 107 258 76 847.3 0 76 259 148 847.3 0 148 260 235 847.3 0 235 261 319 847.3 0 319 262 378 847.3 0 378 263 351 847.3 0 351 264 164 847.3 0 164 265 192 847.3 0 192 266 199 847.3 0 199 267 194 847.3 0 194 268 167 847.3 0 167 269 83 847.3 0 83 270 114 847.3 0 114 271 145 847.3 0 145 272 179 847.3 0 179 273 171 847.3 0 171 274 134 847.3 0 134 275 297 847.3 0 297 276 136 847.3 0 136 277 363 847.3 0 363 278 290 847.3 0 290 279 274 847.3 0 274 280 147 847.3 0 147 281 201 847.3 0 201 282 211 847.3 0 211 283 112 847.3 0 112 284 181 847.3 0 181 285 286 847.3 0 286 286 331 847.3 0 331 287 654 847.3 0 654 288 671 847.3 0 671 289 784 847.3 0 784 290 453 847.3 0 453 291 163 847.3 0 163 292 198 847.3 0 198 293 181 847.3 0 181 294 125 847.3 0 125 295 170 847.3 0 170 296 123 847.3 0 123 297 170 847.3 0 170 298 185 847.3 0 185 299 183 847.3 0 183 300 139 847.3 0 139 301 149 847.3 0 149 Finished in 31.15 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 489,054 Reads with adapters: 68,848 (14.1%) Reads that were too short: 599 (0.1%) Reads written (passing filters): 68,249 (14.0%) Total basepairs processed: 114,788,664 bp Total written (filtered): 18,546,675 bp (16.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 68848 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 32 0.0 3 0 0 21 11 16 636 0.0 3 0 517 79 40 17 65337 0.0 3 61653 3063 505 116 18 160 0.0 3 6 130 11 13 19 180 0.0 3 0 0 108 72 20 69 0.0 3 0 0 0 69 21 9 0.0 3 2 0 0 7 22 1 0.0 3 0 0 1 24 1 0.0 3 0 0 0 1 25 3 0.0 3 1 0 0 2 26 4 0.0 3 0 0 0 4 27 1 0.0 3 0 0 0 1 28 22 0.0 3 0 0 0 22 29 11 0.0 3 0 0 0 11 30 3 0.0 3 0 0 0 3 32 2 0.0 3 0 0 2 33 4 0.0 3 0 0 0 4 35 1 0.0 3 0 0 0 1 38 32 0.0 3 1 0 1 30 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 53 6 0.0 3 0 0 0 6 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 1 57 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 60 6 0.0 3 0 0 0 6 61 17 0.0 3 0 0 0 17 62 6 0.0 3 0 0 0 6 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 95 2 0.0 3 0 0 1 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 103 7 0.0 3 0 0 0 7 104 27 0.0 3 0 1 0 26 105 2 0.0 3 0 0 0 2 106 6 0.0 3 0 0 0 6 107 6 0.0 3 0 0 1 5 108 6 0.0 3 0 0 0 6 109 64 0.0 3 0 0 2 62 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 112 4 0.0 3 0 0 0 4 114 23 0.0 3 0 0 0 23 115 8 0.0 3 0 0 0 8 116 14 0.0 3 0 0 0 14 117 20 0.0 3 0 0 0 20 118 3 0.0 3 0 0 1 2 119 3 0.0 3 0 0 1 2 120 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 7 0.0 3 0 0 0 7 124 12 0.0 3 0 0 0 12 125 4 0.0 3 0 0 0 4 126 3 0.0 3 0 0 0 3 127 4 0.0 3 0 0 0 4 128 21 0.0 3 0 0 0 21 129 1189 0.0 3 0 0 8 1181 130 100 0.0 3 0 0 3 97 131 5 0.0 3 0 0 0 5 132 171 0.0 3 0 0 5 166 133 20 0.0 3 0 1 0 19 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 3 0.0 3 1 0 0 2 143 4 0.0 3 0 0 0 4 144 4 0.0 3 0 0 0 4 145 4 0.0 3 0 0 0 4 146 2 0.0 3 0 0 0 2 148 4 0.0 3 0 1 0 3 149 4 0.0 3 0 0 0 4 150 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 1 3 155 19 0.0 3 0 0 0 19 156 10 0.0 3 0 0 1 9 157 4 0.0 3 0 0 1 3 158 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 1 2 164 12 0.0 3 0 0 1 11 166 1 0.0 3 0 0 0 1 169 4 0.0 3 0 0 0 4 170 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 1 0 1 180 43 0.0 3 0 0 3 40 181 13 0.0 3 0 0 0 13 182 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 3 0.0 3 0 0 0 3 189 21 0.0 3 0 0 0 21 190 6 0.0 3 0 0 0 6 192 11 0.0 3 0 0 0 11 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 196 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 200 3 0.0 3 0 0 0 3 204 3 0.0 3 0 0 0 3 205 4 0.0 3 0 0 0 4 206 6 0.0 3 0 0 0 6 207 3 0.0 3 0 0 0 3 208 7 0.0 3 0 0 1 6 209 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 211 3 0.0 3 0 0 0 3 214 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 15 0.0 3 0 0 0 15 218 2 0.0 3 0 0 0 2 220 7 0.0 3 0 0 0 7 221 8 0.0 3 0 0 0 8 222 4 0.0 3 0 0 0 4 223 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 229 4 0.0 3 0 0 0 4 230 48 0.0 3 0 0 3 45 231 109 0.0 3 0 0 7 102 232 6 0.0 3 0 0 1 5 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 10 0.0 3 0 0 0 10 241 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 1 276 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 1 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 8 0.0 3 0 0 0 8 282 6 0.0 3 0 0 0 6 286 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.86 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 68,249 Reads with adapters: 1,573 (2.3%) Reads written (passing filters): 68,249 (100.0%) Total basepairs processed: 18,546,675 bp Total written (filtered): 18,541,833 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1573 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.6% C: 12.1% G: 7.3% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1461 1066.4 0 1461 4 102 266.6 0 102 5 9 66.6 0 9 6 1 16.7 0 1