This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 163.81 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 78,655 (2.3%) Reads that were too short: 14,009 (0.4%) Reads written (passing filters): 64,646 (1.9%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 13,581,909 bp (1.3%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 78655 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 767 847.3 0 767 7 11580 847.3 0 11580 8 9499 847.3 0 9499 9 135 847.3 0 135 10 106 847.3 0 106 11 55 847.3 0 55 12 78 847.3 0 78 13 65 847.3 0 65 14 78 847.3 0 78 15 73 847.3 0 73 16 85 847.3 0 85 17 106 847.3 0 106 18 101 847.3 0 101 19 138 847.3 0 138 20 161 847.3 0 161 21 294 847.3 0 294 22 125 847.3 0 125 23 91 847.3 0 91 24 57 847.3 0 57 25 59 847.3 0 59 26 122 847.3 0 122 27 84 847.3 0 84 28 66 847.3 0 66 29 87 847.3 0 87 30 103 847.3 0 103 31 66 847.3 0 66 32 67 847.3 0 67 33 76 847.3 0 76 34 115 847.3 0 115 35 90 847.3 0 90 36 75 847.3 0 75 37 90 847.3 0 90 38 112 847.3 0 112 39 168 847.3 0 168 40 151 847.3 0 151 41 305 847.3 0 305 42 86 847.3 0 86 43 63 847.3 0 63 44 86 847.3 0 86 45 94 847.3 0 94 46 75 847.3 0 75 47 91 847.3 0 91 48 109 847.3 0 109 49 103 847.3 0 103 50 93 847.3 0 93 51 83 847.3 0 83 52 75 847.3 0 75 53 100 847.3 0 100 54 67 847.3 0 67 55 78 847.3 0 78 56 98 847.3 0 98 57 104 847.3 0 104 58 105 847.3 0 105 59 59 847.3 0 59 60 79 847.3 0 79 61 73 847.3 0 73 62 70 847.3 0 70 63 62 847.3 0 62 64 94 847.3 0 94 65 90 847.3 0 90 66 69 847.3 0 69 67 86 847.3 0 86 68 76 847.3 0 76 69 79 847.3 0 79 70 94 847.3 0 94 71 85 847.3 0 85 72 81 847.3 0 81 73 97 847.3 0 97 74 80 847.3 0 80 75 91 847.3 0 91 76 153 847.3 0 153 77 139 847.3 0 139 78 291 847.3 0 291 79 229 847.3 0 229 80 126 847.3 0 126 81 202 847.3 0 202 82 86 847.3 0 86 83 73 847.3 0 73 84 104 847.3 0 104 85 103 847.3 0 103 86 76 847.3 0 76 87 80 847.3 0 80 88 96 847.3 0 96 89 170 847.3 0 170 90 125 847.3 0 125 91 148 847.3 0 148 92 165 847.3 0 165 93 680 847.3 0 680 94 503 847.3 0 503 95 247 847.3 0 247 96 269 847.3 0 269 97 237 847.3 0 237 98 276 847.3 0 276 99 88 847.3 0 88 100 81 847.3 0 81 101 79 847.3 0 79 102 72 847.3 0 72 103 49 847.3 0 49 104 76 847.3 0 76 105 86 847.3 0 86 106 114 847.3 0 114 107 117 847.3 0 117 108 107 847.3 0 107 109 71 847.3 0 71 110 100 847.3 0 100 111 76 847.3 0 76 112 72 847.3 0 72 113 221 847.3 0 221 114 123 847.3 0 123 115 134 847.3 0 134 116 157 847.3 0 157 117 183 847.3 0 183 118 71 847.3 0 71 119 59 847.3 0 59 120 86 847.3 0 86 121 74 847.3 0 74 122 92 847.3 0 92 123 118 847.3 0 118 124 58 847.3 0 58 125 118 847.3 0 118 126 106 847.3 0 106 127 138 847.3 0 138 128 181 847.3 0 181 129 232 847.3 0 232 130 1970 847.3 0 1970 131 824 847.3 0 824 132 810 847.3 0 810 133 69 847.3 0 69 134 66 847.3 0 66 135 89 847.3 0 89 136 75 847.3 0 75 137 95 847.3 0 95 138 115 847.3 0 115 139 108 847.3 0 108 140 93 847.3 0 93 141 71 847.3 0 71 142 114 847.3 0 114 143 173 847.3 0 173 144 123 847.3 0 123 145 136 847.3 0 136 146 95 847.3 0 95 147 107 847.3 0 107 148 68 847.3 0 68 149 79 847.3 0 79 150 75 847.3 0 75 151 89 847.3 0 89 152 125 847.3 0 125 153 75 847.3 0 75 154 103 847.3 0 103 155 85 847.3 0 85 156 118 847.3 0 118 157 187 847.3 0 187 158 136 847.3 0 136 159 158 847.3 0 158 160 89 847.3 0 89 161 97 847.3 0 97 162 152 847.3 0 152 163 126 847.3 0 126 164 132 847.3 0 132 165 199 847.3 0 199 166 199 847.3 0 199 167 330 847.3 0 330 168 425 847.3 0 425 169 4052 847.3 0 4052 170 3792 847.3 0 3792 171 9971 847.3 0 9971 172 128 847.3 0 128 173 70 847.3 0 70 174 161 847.3 0 161 175 80 847.3 0 80 176 115 847.3 0 115 177 61 847.3 0 61 178 103 847.3 0 103 179 86 847.3 0 86 180 96 847.3 0 96 181 83 847.3 0 83 182 63 847.3 0 63 183 62 847.3 0 62 184 71 847.3 0 71 185 76 847.3 0 76 186 58 847.3 0 58 187 60 847.3 0 60 188 63 847.3 0 63 189 70 847.3 0 70 190 64 847.3 0 64 191 67 847.3 0 67 192 98 847.3 0 98 193 100 847.3 0 100 194 88 847.3 0 88 195 52 847.3 0 52 196 81 847.3 0 81 197 63 847.3 0 63 198 99 847.3 0 99 199 69 847.3 0 69 200 65 847.3 0 65 201 77 847.3 0 77 202 101 847.3 0 101 203 136 847.3 0 136 204 200 847.3 0 200 205 188 847.3 0 188 206 129 847.3 0 129 207 48 847.3 0 48 208 76 847.3 0 76 209 113 847.3 0 113 210 134 847.3 0 134 211 136 847.3 0 136 212 76 847.3 0 76 213 80 847.3 0 80 214 74 847.3 0 74 215 119 847.3 0 119 216 101 847.3 0 101 217 108 847.3 0 108 218 101 847.3 0 101 219 96 847.3 0 96 220 92 847.3 0 92 221 97 847.3 0 97 222 440 847.3 0 440 223 353 847.3 0 353 224 203 847.3 0 203 225 63 847.3 0 63 226 76 847.3 0 76 227 62 847.3 0 62 228 73 847.3 0 73 229 70 847.3 0 70 230 123 847.3 0 123 231 124 847.3 0 124 232 169 847.3 0 169 233 112 847.3 0 112 234 146 847.3 0 146 235 195 847.3 0 195 236 366 847.3 0 366 237 355 847.3 0 355 238 143 847.3 0 143 239 79 847.3 0 79 240 92 847.3 0 92 241 108 847.3 0 108 242 125 847.3 0 125 243 111 847.3 0 111 244 71 847.3 0 71 245 88 847.3 0 88 246 88 847.3 0 88 247 82 847.3 0 82 248 66 847.3 0 66 249 72 847.3 0 72 250 90 847.3 0 90 251 95 847.3 0 95 252 91 847.3 0 91 253 124 847.3 0 124 254 141 847.3 0 141 255 157 847.3 0 157 256 147 847.3 0 147 257 91 847.3 0 91 258 115 847.3 0 115 259 75 847.3 0 75 260 70 847.3 0 70 261 183 847.3 0 183 262 215 847.3 0 215 263 136 847.3 0 136 264 91 847.3 0 91 265 78 847.3 0 78 266 90 847.3 0 90 267 82 847.3 0 82 268 74 847.3 0 74 269 78 847.3 0 78 270 106 847.3 0 106 271 282 847.3 0 282 272 288 847.3 0 288 273 303 847.3 0 303 274 121 847.3 0 121 275 73 847.3 0 73 276 112 847.3 0 112 277 140 847.3 0 140 278 178 847.3 0 178 279 123 847.3 0 123 280 91 847.3 0 91 281 116 847.3 0 116 282 108 847.3 0 108 283 118 847.3 0 118 284 81 847.3 0 81 285 93 847.3 0 93 286 86 847.3 0 86 287 72 847.3 0 72 288 92 847.3 0 92 289 105 847.3 0 105 290 132 847.3 0 132 291 90 847.3 0 90 292 275 847.3 0 275 293 283 847.3 0 283 294 239 847.3 0 239 295 167 847.3 0 167 296 642 847.3 0 642 297 403 847.3 0 403 298 251 847.3 0 251 299 169 847.3 0 169 300 113 847.3 0 113 301 74 847.3 0 74 Finished in 4.03 s (62 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 64,646 Reads with adapters: 11,725 (18.1%) Reads that were too short: 45 (0.1%) Reads written (passing filters): 11,680 (18.1%) Total basepairs processed: 13,581,909 bp Total written (filtered): 3,225,315 bp (23.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11725 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 110 0.0 3 0 98 10 2 17 11141 0.0 3 10530 528 70 13 18 32 0.0 3 1 27 1 3 19 17 0.0 3 1 2 10 4 20 306 0.0 3 268 11 2 25 23 2 0.0 3 1 0 0 1 24 1 0.0 3 1 28 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 42 5 0.0 3 0 0 0 5 44 5 0.0 3 0 0 0 5 46 11 0.0 3 0 0 0 11 47 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 62 8 0.0 3 0 0 0 8 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 7 0.0 3 0 0 0 7 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 1 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 217 9 0.0 3 0 1 0 8 254 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 11,680 Reads with adapters: 63 (0.5%) Reads written (passing filters): 11,680 (100.0%) Total basepairs processed: 3,225,315 bp Total written (filtered): 3,225,120 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 63 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.6% C: 44.4% G: 20.6% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 60 182.5 0 60 4 2 45.6 0 2 7 1 0.7 0 1