This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.95 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 35,285 (1.4%) Reads that were too short: 10,271 (0.4%) Reads written (passing filters): 25,014 (1.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 5,895,363 bp (0.8%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 35285 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3027 601.0 0 3027 7 188 601.0 0 188 8 5511 601.0 0 5511 9 4120 601.0 0 4120 10 20 601.0 0 20 11 24 601.0 0 24 12 25 601.0 0 25 13 37 601.0 0 37 14 28 601.0 0 28 15 31 601.0 0 31 16 10 601.0 0 10 17 13 601.0 0 13 18 32 601.0 0 32 19 26 601.0 0 26 20 23 601.0 0 23 21 25 601.0 0 25 22 43 601.0 0 43 23 34 601.0 0 34 24 25 601.0 0 25 25 21 601.0 0 21 26 11 601.0 0 11 27 26 601.0 0 26 28 24 601.0 0 24 29 30 601.0 0 30 30 26 601.0 0 26 31 26 601.0 0 26 32 19 601.0 0 19 33 16 601.0 0 16 34 23 601.0 0 23 35 34 601.0 0 34 36 18 601.0 0 18 37 50 601.0 0 50 38 73 601.0 0 73 39 35 601.0 0 35 40 29 601.0 0 29 41 41 601.0 0 41 42 24 601.0 0 24 43 43 601.0 0 43 44 20 601.0 0 20 45 52 601.0 0 52 46 25 601.0 0 25 47 47 601.0 0 47 48 486 601.0 0 486 49 441 601.0 0 441 50 487 601.0 0 487 51 40 601.0 0 40 52 22 601.0 0 22 53 31 601.0 0 31 54 13 601.0 0 13 55 31 601.0 0 31 56 22 601.0 0 22 57 20 601.0 0 20 58 21 601.0 0 21 59 25 601.0 0 25 60 28 601.0 0 28 61 24 601.0 0 24 62 25 601.0 0 25 63 14 601.0 0 14 64 30 601.0 0 30 65 12 601.0 0 12 66 19 601.0 0 19 67 24 601.0 0 24 68 20 601.0 0 20 69 39 601.0 0 39 70 32 601.0 0 32 71 14 601.0 0 14 72 10 601.0 0 10 73 21 601.0 0 21 74 23 601.0 0 23 75 25 601.0 0 25 76 30 601.0 0 30 77 33 601.0 0 33 78 20 601.0 0 20 79 14 601.0 0 14 80 40 601.0 0 40 81 77 601.0 0 77 82 146 601.0 0 146 83 99 601.0 0 99 84 34 601.0 0 34 85 29 601.0 0 29 86 23 601.0 0 23 87 13 601.0 0 13 88 26 601.0 0 26 89 59 601.0 0 59 90 24 601.0 0 24 91 15 601.0 0 15 92 30 601.0 0 30 93 20 601.0 0 20 94 18 601.0 0 18 95 15 601.0 0 15 96 68 601.0 0 68 97 126 601.0 0 126 98 66 601.0 0 66 99 19 601.0 0 19 100 23 601.0 0 23 101 17 601.0 0 17 102 11 601.0 0 11 103 17 601.0 0 17 104 37 601.0 0 37 105 33 601.0 0 33 106 22 601.0 0 22 107 13 601.0 0 13 108 23 601.0 0 23 109 31 601.0 0 31 110 29 601.0 0 29 111 21 601.0 0 21 112 26 601.0 0 26 113 43 601.0 0 43 114 57 601.0 0 57 115 28 601.0 0 28 116 24 601.0 0 24 117 74 601.0 0 74 118 492 601.0 0 492 119 279 601.0 0 279 120 103 601.0 0 103 121 17 601.0 0 17 122 35 601.0 0 35 123 25 601.0 0 25 124 23 601.0 0 23 125 34 601.0 0 34 126 27 601.0 0 27 127 74 601.0 0 74 128 62 601.0 0 62 129 36 601.0 0 36 130 32 601.0 0 32 131 28 601.0 0 28 132 46 601.0 0 46 133 42 601.0 0 42 134 26 601.0 0 26 135 43 601.0 0 43 136 172 601.0 0 172 137 108 601.0 0 108 138 66 601.0 0 66 139 25 601.0 0 25 140 45 601.0 0 45 141 36 601.0 0 36 142 102 601.0 0 102 143 62 601.0 0 62 144 77 601.0 0 77 145 66 601.0 0 66 146 146 601.0 0 146 147 110 601.0 0 110 148 435 601.0 0 435 149 80 601.0 0 80 150 61 601.0 0 61 151 37 601.0 0 37 152 41 601.0 0 41 153 106 601.0 0 106 154 48 601.0 0 48 155 28 601.0 0 28 156 30 601.0 0 30 157 36 601.0 0 36 158 45 601.0 0 45 159 16 601.0 0 16 160 25 601.0 0 25 161 21 601.0 0 21 162 20 601.0 0 20 163 28 601.0 0 28 164 25 601.0 0 25 165 25 601.0 0 25 166 25 601.0 0 25 167 23 601.0 0 23 168 27 601.0 0 27 169 18 601.0 0 18 170 22 601.0 0 22 171 20 601.0 0 20 172 44 601.0 0 44 173 22 601.0 0 22 174 18 601.0 0 18 175 22 601.0 0 22 176 30 601.0 0 30 177 39 601.0 0 39 178 52 601.0 0 52 179 34 601.0 0 34 180 34 601.0 0 34 181 40 601.0 0 40 182 49 601.0 0 49 183 67 601.0 0 67 184 20 601.0 0 20 185 37 601.0 0 37 186 22 601.0 0 22 187 30 601.0 0 30 188 52 601.0 0 52 189 65 601.0 0 65 190 60 601.0 0 60 191 134 601.0 0 134 192 58 601.0 0 58 193 92 601.0 0 92 194 222 601.0 0 222 195 659 601.0 0 659 196 841 601.0 0 841 197 866 601.0 0 866 198 57 601.0 0 57 199 45 601.0 0 45 200 49 601.0 0 49 201 46 601.0 0 46 202 40 601.0 0 40 203 40 601.0 0 40 204 32 601.0 0 32 205 20 601.0 0 20 206 35 601.0 0 35 207 19 601.0 0 19 208 34 601.0 0 34 209 36 601.0 0 36 210 46 601.0 0 46 211 28 601.0 0 28 212 48 601.0 0 48 213 82 601.0 0 82 214 90 601.0 0 90 215 143 601.0 0 143 216 112 601.0 0 112 217 37 601.0 0 37 218 73 601.0 0 73 219 54 601.0 0 54 220 51 601.0 0 51 221 55 601.0 0 55 222 53 601.0 0 53 223 29 601.0 0 29 224 57 601.0 0 57 225 41 601.0 0 41 226 37 601.0 0 37 227 21 601.0 0 21 228 28 601.0 0 28 229 54 601.0 0 54 230 79 601.0 0 79 231 59 601.0 0 59 232 53 601.0 0 53 233 75 601.0 0 75 234 78 601.0 0 78 235 97 601.0 0 97 236 86 601.0 0 86 237 62 601.0 0 62 238 62 601.0 0 62 239 116 601.0 0 116 240 153 601.0 0 153 241 105 601.0 0 105 242 72 601.0 0 72 243 534 601.0 0 534 244 312 601.0 0 312 245 228 601.0 0 228 246 82 601.0 0 82 247 61 601.0 0 61 248 67 601.0 0 67 249 158 601.0 0 158 250 148 601.0 0 148 251 166 601.0 0 166 252 225 601.0 0 225 253 136 601.0 0 136 254 59 601.0 0 59 255 49 601.0 0 49 256 75 601.0 0 75 257 83 601.0 0 83 258 64 601.0 0 64 259 86 601.0 0 86 260 143 601.0 0 143 261 439 601.0 0 439 262 197 601.0 0 197 263 122 601.0 0 122 264 74 601.0 0 74 265 77 601.0 0 77 266 57 601.0 0 57 267 43 601.0 0 43 268 73 601.0 0 73 269 165 601.0 0 165 270 180 601.0 0 180 271 95 601.0 0 95 272 84 601.0 0 84 273 51 601.0 0 51 274 46 601.0 0 46 275 53 601.0 0 53 276 53 601.0 0 53 277 51 601.0 0 51 278 83 601.0 0 83 279 53 601.0 0 53 280 96 601.0 0 96 281 67 601.0 0 67 282 117 601.0 0 117 283 60 601.0 0 60 284 125 601.0 0 125 285 120 601.0 0 120 286 88 601.0 0 88 287 187 601.0 0 187 288 155 601.0 0 155 289 249 601.0 0 249 290 167 601.0 0 167 291 147 601.0 0 147 292 122 601.0 0 122 293 104 601.0 0 104 294 94 601.0 0 94 295 110 601.0 0 110 296 147 601.0 0 147 297 379 601.0 0 379 298 213 601.0 0 213 299 182 601.0 0 182 300 141 601.0 0 141 301 37 601.0 0 37 Finished in 1.24 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 25,014 Reads with adapters: 8,239 (32.9%) Reads that were too short: 85 (0.3%) Reads written (passing filters): 8,154 (32.6%) Total basepairs processed: 5,895,363 bp Total written (filtered): 2,223,948 bp (37.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8239 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 41 0.0 3 0 36 3 2 17 5033 0.0 3 4561 381 72 19 18 21 0.0 3 1 17 3 19 66 0.0 3 11 35 12 8 20 2801 0.0 3 2492 208 46 55 21 7 0.0 3 1 4 1 1 22 12 0.0 3 0 0 5 7 23 10 0.0 3 0 0 0 10 26 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 27 0.0 3 0 0 1 26 30 1 0.0 3 0 0 0 1 31 7 0.0 3 0 0 0 7 32 4 0.0 3 0 0 0 4 34 2 0.0 3 0 0 0 2 35 11 0.0 3 0 0 0 11 38 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 5 0.0 3 0 0 0 5 45 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 53 5 0.0 3 0 0 0 5 54 8 0.0 3 0 0 0 8 55 2 0.0 3 0 0 0 2 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 4 0.0 3 0 0 0 4 62 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 1 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 6 0.0 3 0 0 0 6 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 1 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 1 2 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 1 2 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 126 3 0.0 3 0 0 1 2 132 1 0.0 3 0 0 0 1 137 4 0.0 3 0 0 0 4 138 1 0.0 3 0 0 0 1 141 4 0.0 3 0 0 0 4 142 2 0.0 3 0 0 0 2 143 11 0.0 3 0 0 1 10 144 5 0.0 3 0 0 0 5 146 6 0.0 3 0 0 1 5 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 1 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 4 0.0 3 0 0 0 4 166 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 8,154 Reads with adapters: 66 (0.8%) Reads written (passing filters): 8,154 (100.0%) Total basepairs processed: 2,223,948 bp Total written (filtered): 2,223,724 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 66 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 15.2% G: 47.0% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 42 127.4 0 42 4 22 31.9 0 22 5 2 8.0 0 2