This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.99 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 22,068 (0.9%) Reads that were too short: 3,698 (0.2%) Reads written (passing filters): 18,370 (0.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 4,762,966 bp (0.6%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 22068 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2622 601.0 0 2622 7 146 601.0 0 146 8 5296 601.0 0 5296 9 4950 601.0 0 4950 10 11 601.0 0 11 11 4 601.0 0 4 12 7 601.0 0 7 13 13 601.0 0 13 14 15 601.0 0 15 15 13 601.0 0 13 16 17 601.0 0 17 17 14 601.0 0 14 18 19 601.0 0 19 19 13 601.0 0 13 20 8 601.0 0 8 21 12 601.0 0 12 22 24 601.0 0 24 23 11 601.0 0 11 24 12 601.0 0 12 25 9 601.0 0 9 26 14 601.0 0 14 27 22 601.0 0 22 28 13 601.0 0 13 29 25 601.0 0 25 30 14 601.0 0 14 31 11 601.0 0 11 32 10 601.0 0 10 33 15 601.0 0 15 34 19 601.0 0 19 35 15 601.0 0 15 36 12 601.0 0 12 37 22 601.0 0 22 38 27 601.0 0 27 39 15 601.0 0 15 40 20 601.0 0 20 41 26 601.0 0 26 42 16 601.0 0 16 43 20 601.0 0 20 44 14 601.0 0 14 45 15 601.0 0 15 46 18 601.0 0 18 47 21 601.0 0 21 48 201 601.0 0 201 49 74 601.0 0 74 50 231 601.0 0 231 51 7 601.0 0 7 52 15 601.0 0 15 53 15 601.0 0 15 54 10 601.0 0 10 55 17 601.0 0 17 56 16 601.0 0 16 57 12 601.0 0 12 58 10 601.0 0 10 59 16 601.0 0 16 60 11 601.0 0 11 61 14 601.0 0 14 62 16 601.0 0 16 63 7 601.0 0 7 64 14 601.0 0 14 65 14 601.0 0 14 66 6 601.0 0 6 67 18 601.0 0 18 68 11 601.0 0 11 69 26 601.0 0 26 70 24 601.0 0 24 71 10 601.0 0 10 72 6 601.0 0 6 73 19 601.0 0 19 74 13 601.0 0 13 75 11 601.0 0 11 76 11 601.0 0 11 77 10 601.0 0 10 78 3 601.0 0 3 79 9 601.0 0 9 80 22 601.0 0 22 81 16 601.0 0 16 82 34 601.0 0 34 83 32 601.0 0 32 84 20 601.0 0 20 85 39 601.0 0 39 86 14 601.0 0 14 87 18 601.0 0 18 88 14 601.0 0 14 89 13 601.0 0 13 90 17 601.0 0 17 91 9 601.0 0 9 92 23 601.0 0 23 93 16 601.0 0 16 94 19 601.0 0 19 95 16 601.0 0 16 96 30 601.0 0 30 97 37 601.0 0 37 98 28 601.0 0 28 99 20 601.0 0 20 100 13 601.0 0 13 101 8 601.0 0 8 102 12 601.0 0 12 103 9 601.0 0 9 104 18 601.0 0 18 105 19 601.0 0 19 106 12 601.0 0 12 107 17 601.0 0 17 108 14 601.0 0 14 109 23 601.0 0 23 110 13 601.0 0 13 111 9 601.0 0 9 112 9 601.0 0 9 113 11 601.0 0 11 114 26 601.0 0 26 115 32 601.0 0 32 116 20 601.0 0 20 117 30 601.0 0 30 118 124 601.0 0 124 119 69 601.0 0 69 120 28 601.0 0 28 121 13 601.0 0 13 122 32 601.0 0 32 123 16 601.0 0 16 124 18 601.0 0 18 125 13 601.0 0 13 126 17 601.0 0 17 127 31 601.0 0 31 128 48 601.0 0 48 129 11 601.0 0 11 130 19 601.0 0 19 131 7 601.0 0 7 132 24 601.0 0 24 133 17 601.0 0 17 134 15 601.0 0 15 135 17 601.0 0 17 136 41 601.0 0 41 137 30 601.0 0 30 138 31 601.0 0 31 139 17 601.0 0 17 140 22 601.0 0 22 141 18 601.0 0 18 142 31 601.0 0 31 143 22 601.0 0 22 144 21 601.0 0 21 145 24 601.0 0 24 146 104 601.0 0 104 147 63 601.0 0 63 148 174 601.0 0 174 149 32 601.0 0 32 150 17 601.0 0 17 151 31 601.0 0 31 152 35 601.0 0 35 153 119 601.0 0 119 154 17 601.0 0 17 155 14 601.0 0 14 156 26 601.0 0 26 157 13 601.0 0 13 158 18 601.0 0 18 159 10 601.0 0 10 160 14 601.0 0 14 161 14 601.0 0 14 162 17 601.0 0 17 163 19 601.0 0 19 164 15 601.0 0 15 165 16 601.0 0 16 166 11 601.0 0 11 167 22 601.0 0 22 168 15 601.0 0 15 169 14 601.0 0 14 170 19 601.0 0 19 171 19 601.0 0 19 172 13 601.0 0 13 173 15 601.0 0 15 174 15 601.0 0 15 175 16 601.0 0 16 176 16 601.0 0 16 177 21 601.0 0 21 178 20 601.0 0 20 179 19 601.0 0 19 180 21 601.0 0 21 181 24 601.0 0 24 182 36 601.0 0 36 183 64 601.0 0 64 184 13 601.0 0 13 185 10 601.0 0 10 186 7 601.0 0 7 187 16 601.0 0 16 188 19 601.0 0 19 189 30 601.0 0 30 190 17 601.0 0 17 191 98 601.0 0 98 192 41 601.0 0 41 193 41 601.0 0 41 194 39 601.0 0 39 195 231 601.0 0 231 196 308 601.0 0 308 197 302 601.0 0 302 198 21 601.0 0 21 199 13 601.0 0 13 200 15 601.0 0 15 201 16 601.0 0 16 202 11 601.0 0 11 203 17 601.0 0 17 204 14 601.0 0 14 205 10 601.0 0 10 206 9 601.0 0 9 207 13 601.0 0 13 208 15 601.0 0 15 209 23 601.0 0 23 210 20 601.0 0 20 211 18 601.0 0 18 212 9 601.0 0 9 213 15 601.0 0 15 214 15 601.0 0 15 215 36 601.0 0 36 216 37 601.0 0 37 217 12 601.0 0 12 218 58 601.0 0 58 219 20 601.0 0 20 220 11 601.0 0 11 221 28 601.0 0 28 222 11 601.0 0 11 223 11 601.0 0 11 224 15 601.0 0 15 225 21 601.0 0 21 226 19 601.0 0 19 227 13 601.0 0 13 228 18 601.0 0 18 229 20 601.0 0 20 230 22 601.0 0 22 231 19 601.0 0 19 232 21 601.0 0 21 233 18 601.0 0 18 234 29 601.0 0 29 235 37 601.0 0 37 236 19 601.0 0 19 237 21 601.0 0 21 238 24 601.0 0 24 239 29 601.0 0 29 240 35 601.0 0 35 241 33 601.0 0 33 242 16 601.0 0 16 243 121 601.0 0 121 244 78 601.0 0 78 245 62 601.0 0 62 246 32 601.0 0 32 247 17 601.0 0 17 248 28 601.0 0 28 249 38 601.0 0 38 250 56 601.0 0 56 251 193 601.0 0 193 252 30 601.0 0 30 253 19 601.0 0 19 254 22 601.0 0 22 255 19 601.0 0 19 256 22 601.0 0 22 257 39 601.0 0 39 258 22 601.0 0 22 259 41 601.0 0 41 260 49 601.0 0 49 261 111 601.0 0 111 262 52 601.0 0 52 263 38 601.0 0 38 264 14 601.0 0 14 265 15 601.0 0 15 266 20 601.0 0 20 267 16 601.0 0 16 268 14 601.0 0 14 269 44 601.0 0 44 270 37 601.0 0 37 271 50 601.0 0 50 272 25 601.0 0 25 273 18 601.0 0 18 274 16 601.0 0 16 275 29 601.0 0 29 276 19 601.0 0 19 277 19 601.0 0 19 278 25 601.0 0 25 279 30 601.0 0 30 280 23 601.0 0 23 281 27 601.0 0 27 282 35 601.0 0 35 283 28 601.0 0 28 284 44 601.0 0 44 285 65 601.0 0 65 286 45 601.0 0 45 287 230 601.0 0 230 288 108 601.0 0 108 289 146 601.0 0 146 290 58 601.0 0 58 291 42 601.0 0 42 292 43 601.0 0 43 293 41 601.0 0 41 294 36 601.0 0 36 295 34 601.0 0 34 296 47 601.0 0 47 297 102 601.0 0 102 298 74 601.0 0 74 299 66 601.0 0 66 300 48 601.0 0 48 301 34 601.0 0 34 Finished in 1.05 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 18,370 Reads with adapters: 5,449 (29.7%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 5,444 (29.6%) Total basepairs processed: 4,762,966 bp Total written (filtered): 1,476,168 bp (31.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5449 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 99 0.0 3 0 98 0 1 20 5264 0.0 4 5193 65 5 0 1 21 16 0.0 4 4 12 22 5 0.0 4 2 0 2 1 23 7 0.0 4 1 0 0 6 38 3 0.0 4 1 0 0 0 2 86 1 0.0 4 0 0 0 0 1 130 46 0.0 4 0 0 0 0 46 140 1 0.0 4 1 154 1 0.0 4 0 0 0 0 1 190 1 0.0 4 1 231 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (39 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 5,444 Reads with adapters: 650 (11.9%) Reads written (passing filters): 5,444 (100.0%) Total basepairs processed: 1,476,168 bp Total written (filtered): 1,468,399 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 650 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 3.5% G: 94.3% T: 0.8% none/other: 0.6% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 85.1 0 4 4 2 21.3 0 2 9 277 0.0 0 12 265 10 169 0.0 1 6 163 11 190 0.0 1 0 190 12 1 0.0 1 0 1 113 2 0.0 1 2 150 1 0.0 1 0 1 272 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.