This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.15 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 68,696 (2.8%) Reads that were too short: 27,815 (1.1%) Reads written (passing filters): 40,881 (1.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,188,498 bp (1.1%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 68696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 312 601.0 0 312 7 1876 601.0 0 1876 8 1651 601.0 0 1651 9 84 601.0 0 84 10 124 601.0 0 124 11 69 601.0 0 69 12 56 601.0 0 56 13 69 601.0 0 69 14 73 601.0 0 73 15 65 601.0 0 65 16 50 601.0 0 50 17 79 601.0 0 79 18 92 601.0 0 92 19 240 601.0 0 240 20 61 601.0 0 61 21 48 601.0 0 48 22 43 601.0 0 43 23 47 601.0 0 47 24 46 601.0 0 46 25 53 601.0 0 53 26 65 601.0 0 65 27 55 601.0 0 55 28 70 601.0 0 70 29 76 601.0 0 76 30 60 601.0 0 60 31 44 601.0 0 44 32 48 601.0 0 48 33 52 601.0 0 52 34 57 601.0 0 57 35 61 601.0 0 61 36 69 601.0 0 69 37 55 601.0 0 55 38 49 601.0 0 49 39 73 601.0 0 73 40 70 601.0 0 70 41 59 601.0 0 59 42 84 601.0 0 84 43 83 601.0 0 83 44 57 601.0 0 57 45 40 601.0 0 40 46 102 601.0 0 102 47 110 601.0 0 110 48 82 601.0 0 82 49 120 601.0 0 120 50 84 601.0 0 84 51 57 601.0 0 57 52 90 601.0 0 90 53 143 601.0 0 143 54 109 601.0 0 109 55 91 601.0 0 91 56 82 601.0 0 82 57 93 601.0 0 93 58 87 601.0 0 87 59 84 601.0 0 84 60 168 601.0 0 168 61 137 601.0 0 137 62 211 601.0 0 211 63 118 601.0 0 118 64 100 601.0 0 100 65 83 601.0 0 83 66 81 601.0 0 81 67 101 601.0 0 101 68 83 601.0 0 83 69 66 601.0 0 66 70 102 601.0 0 102 71 143 601.0 0 143 72 176 601.0 0 176 73 1204 601.0 0 1204 74 1080 601.0 0 1080 75 661 601.0 0 661 76 264 601.0 0 264 77 5454 601.0 0 5454 78 2228 601.0 0 2228 79 1183 601.0 0 1183 80 56 601.0 0 56 81 75 601.0 0 75 82 66 601.0 0 66 83 43 601.0 0 43 84 62 601.0 0 62 85 58 601.0 0 58 86 71 601.0 0 71 87 46 601.0 0 46 88 80 601.0 0 80 89 69 601.0 0 69 90 78 601.0 0 78 91 61 601.0 0 61 92 94 601.0 0 94 93 74 601.0 0 74 94 64 601.0 0 64 95 72 601.0 0 72 96 72 601.0 0 72 97 64 601.0 0 64 98 69 601.0 0 69 99 107 601.0 0 107 100 71 601.0 0 71 101 70 601.0 0 70 102 46 601.0 0 46 103 51 601.0 0 51 104 80 601.0 0 80 105 75 601.0 0 75 106 117 601.0 0 117 107 266 601.0 0 266 108 281 601.0 0 281 109 72 601.0 0 72 110 68 601.0 0 68 111 46 601.0 0 46 112 130 601.0 0 130 113 70 601.0 0 70 114 76 601.0 0 76 115 95 601.0 0 95 116 91 601.0 0 91 117 82 601.0 0 82 118 65 601.0 0 65 119 66 601.0 0 66 120 81 601.0 0 81 121 74 601.0 0 74 122 72 601.0 0 72 123 59 601.0 0 59 124 90 601.0 0 90 125 90 601.0 0 90 126 80 601.0 0 80 127 120 601.0 0 120 128 524 601.0 0 524 129 408 601.0 0 408 130 264 601.0 0 264 131 106 601.0 0 106 132 87 601.0 0 87 133 140 601.0 0 140 134 228 601.0 0 228 135 263 601.0 0 263 136 518 601.0 0 518 137 164 601.0 0 164 138 125 601.0 0 125 139 120 601.0 0 120 140 109 601.0 0 109 141 80 601.0 0 80 142 132 601.0 0 132 143 102 601.0 0 102 144 75 601.0 0 75 145 172 601.0 0 172 146 2103 601.0 0 2103 147 926 601.0 0 926 148 348 601.0 0 348 149 259 601.0 0 259 150 138 601.0 0 138 151 75 601.0 0 75 152 75 601.0 0 75 153 77 601.0 0 77 154 68 601.0 0 68 155 145 601.0 0 145 156 173 601.0 0 173 157 199 601.0 0 199 158 107 601.0 0 107 159 232 601.0 0 232 160 205 601.0 0 205 161 178 601.0 0 178 162 86 601.0 0 86 163 73 601.0 0 73 164 381 601.0 0 381 165 235 601.0 0 235 166 178 601.0 0 178 167 110 601.0 0 110 168 100 601.0 0 100 169 70 601.0 0 70 170 105 601.0 0 105 171 80 601.0 0 80 172 58 601.0 0 58 173 65 601.0 0 65 174 64 601.0 0 64 175 72 601.0 0 72 176 52 601.0 0 52 177 72 601.0 0 72 178 62 601.0 0 62 179 102 601.0 0 102 180 93 601.0 0 93 181 105 601.0 0 105 182 75 601.0 0 75 183 75 601.0 0 75 184 398 601.0 0 398 185 403 601.0 0 403 186 410 601.0 0 410 187 144 601.0 0 144 188 115 601.0 0 115 189 61 601.0 0 61 190 55 601.0 0 55 191 98 601.0 0 98 192 107 601.0 0 107 193 110 601.0 0 110 194 108 601.0 0 108 195 828 601.0 0 828 196 641 601.0 0 641 197 308 601.0 0 308 198 133 601.0 0 133 199 140 601.0 0 140 200 120 601.0 0 120 201 157 601.0 0 157 202 77 601.0 0 77 203 101 601.0 0 101 204 75 601.0 0 75 205 118 601.0 0 118 206 173 601.0 0 173 207 75 601.0 0 75 208 95 601.0 0 95 209 78 601.0 0 78 210 88 601.0 0 88 211 108 601.0 0 108 212 126 601.0 0 126 213 128 601.0 0 128 214 132 601.0 0 132 215 213 601.0 0 213 216 283 601.0 0 283 217 301 601.0 0 301 218 130 601.0 0 130 219 185 601.0 0 185 220 124 601.0 0 124 221 103 601.0 0 103 222 64 601.0 0 64 223 103 601.0 0 103 224 85 601.0 0 85 225 195 601.0 0 195 226 192 601.0 0 192 227 130 601.0 0 130 228 81 601.0 0 81 229 82 601.0 0 82 230 120 601.0 0 120 231 93 601.0 0 93 232 91 601.0 0 91 233 111 601.0 0 111 234 167 601.0 0 167 235 253 601.0 0 253 236 1107 601.0 0 1107 237 1690 601.0 0 1690 238 2610 601.0 0 2610 239 205 601.0 0 205 240 132 601.0 0 132 241 162 601.0 0 162 242 98 601.0 0 98 243 85 601.0 0 85 244 127 601.0 0 127 245 195 601.0 0 195 246 436 601.0 0 436 247 593 601.0 0 593 248 446 601.0 0 446 249 218 601.0 0 218 250 398 601.0 0 398 251 496 601.0 0 496 252 589 601.0 0 589 253 573 601.0 0 573 254 235 601.0 0 235 255 155 601.0 0 155 256 106 601.0 0 106 257 102 601.0 0 102 258 112 601.0 0 112 259 142 601.0 0 142 260 80 601.0 0 80 261 112 601.0 0 112 262 102 601.0 0 102 263 159 601.0 0 159 264 141 601.0 0 141 265 93 601.0 0 93 266 96 601.0 0 96 267 96 601.0 0 96 268 120 601.0 0 120 269 81 601.0 0 81 270 83 601.0 0 83 271 83 601.0 0 83 272 139 601.0 0 139 273 150 601.0 0 150 274 134 601.0 0 134 275 94 601.0 0 94 276 164 601.0 0 164 277 163 601.0 0 163 278 235 601.0 0 235 279 239 601.0 0 239 280 293 601.0 0 293 281 624 601.0 0 624 282 423 601.0 0 423 283 289 601.0 0 289 284 752 601.0 0 752 285 492 601.0 0 492 286 291 601.0 0 291 287 144 601.0 0 144 288 158 601.0 0 158 289 186 601.0 0 186 290 130 601.0 0 130 291 124 601.0 0 124 292 169 601.0 0 169 293 319 601.0 0 319 294 317 601.0 0 317 295 335 601.0 0 335 296 782 601.0 0 782 297 979 601.0 0 979 298 1238 601.0 0 1238 299 667 601.0 0 667 300 442 601.0 0 442 301 605 601.0 0 605 Finished in 1.75 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 40,881 Reads with adapters: 2,655 (6.5%) Reads that were too short: 230 (0.6%) Reads written (passing filters): 2,425 (5.9%) Total basepairs processed: 8,188,498 bp Total written (filtered): 582,987 bp (7.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2655 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 0 2 16 14 0.0 3 0 9 1 4 17 1815 0.0 3 1596 156 51 12 18 10 0.0 3 3 5 1 1 19 14 0.0 3 1 1 7 5 20 8 0.0 3 0 0 0 8 21 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 27 4 0.0 3 1 0 0 3 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 57 0.0 3 0 0 1 56 42 2 0.0 3 0 0 0 2 43 12 0.0 3 0 0 0 12 44 2 0.0 3 0 0 0 2 45 65 0.0 3 0 0 0 65 46 15 0.0 3 0 0 0 15 47 3 0.0 3 0 0 0 3 48 4 0.0 3 0 0 0 4 49 8 0.0 3 0 0 0 8 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 6 0.0 3 0 0 0 6 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 12 0.0 3 0 0 2 10 90 1 0.0 3 0 0 0 1 91 4 0.0 3 0 0 0 4 92 7 0.0 3 0 0 0 7 93 3 0.0 3 0 0 0 3 94 7 0.0 3 0 0 0 7 95 3 0.0 3 0 0 0 3 96 2 0.0 3 0 0 0 2 97 9 0.0 3 0 0 0 9 98 6 0.0 3 0 0 1 5 99 5 0.0 3 0 0 1 4 100 2 0.0 3 0 0 0 2 101 4 0.0 3 0 0 0 4 102 6 0.0 3 0 0 0 6 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 107 2 0.0 3 0 0 1 1 108 7 0.0 3 0 0 0 7 109 4 0.0 3 0 0 0 4 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 220 0.0 3 0 0 1 219 115 32 0.0 3 0 0 0 32 117 26 0.0 3 0 0 1 25 118 32 0.0 3 0 0 0 32 119 12 0.0 3 0 0 0 12 120 2 0.0 3 0 0 0 2 121 5 0.0 3 0 0 0 5 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 4 0.0 3 0 0 0 4 138 1 0.0 3 0 0 0 1 140 5 0.0 3 0 0 0 5 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 151 2 0.0 3 0 0 1 1 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 1 1 154 1 0.0 3 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 16 0.0 3 0 0 0 16 166 4 0.0 3 0 0 0 4 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 7 0.0 3 0 0 0 7 175 5 0.0 3 0 0 0 5 178 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 182 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 1 1 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 214 2 0.0 3 0 0 0 2 215 10 0.0 3 0 0 0 10 216 3 0.0 3 0 0 0 3 217 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 1 234 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 2,425 Reads with adapters: 4 (0.2%) Reads written (passing filters): 2,425 (100.0%) Total basepairs processed: 582,987 bp Total written (filtered): 582,975 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 50.0% G: 25.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 37.9 0 4