This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.23 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 44,570 (1.8%) Reads that were too short: 17,067 (0.7%) Reads written (passing filters): 27,503 (1.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 5,615,023 bp (0.8%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 44570 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 322 601.0 0 322 7 1726 601.0 0 1726 8 2021 601.0 0 2021 9 69 601.0 0 69 10 89 601.0 0 89 11 53 601.0 0 53 12 43 601.0 0 43 13 48 601.0 0 48 14 44 601.0 0 44 15 52 601.0 0 52 16 59 601.0 0 59 17 50 601.0 0 50 18 63 601.0 0 63 19 69 601.0 0 69 20 53 601.0 0 53 21 44 601.0 0 44 22 38 601.0 0 38 23 49 601.0 0 49 24 53 601.0 0 53 25 54 601.0 0 54 26 45 601.0 0 45 27 47 601.0 0 47 28 53 601.0 0 53 29 73 601.0 0 73 30 65 601.0 0 65 31 51 601.0 0 51 32 57 601.0 0 57 33 75 601.0 0 75 34 48 601.0 0 48 35 55 601.0 0 55 36 66 601.0 0 66 37 58 601.0 0 58 38 58 601.0 0 58 39 68 601.0 0 68 40 71 601.0 0 71 41 48 601.0 0 48 42 80 601.0 0 80 43 78 601.0 0 78 44 60 601.0 0 60 45 43 601.0 0 43 46 105 601.0 0 105 47 87 601.0 0 87 48 47 601.0 0 47 49 82 601.0 0 82 50 61 601.0 0 61 51 39 601.0 0 39 52 71 601.0 0 71 53 101 601.0 0 101 54 64 601.0 0 64 55 94 601.0 0 94 56 76 601.0 0 76 57 106 601.0 0 106 58 64 601.0 0 64 59 67 601.0 0 67 60 105 601.0 0 105 61 94 601.0 0 94 62 91 601.0 0 91 63 76 601.0 0 76 64 231 601.0 0 231 65 92 601.0 0 92 66 65 601.0 0 65 67 99 601.0 0 99 68 62 601.0 0 62 69 62 601.0 0 62 70 91 601.0 0 91 71 100 601.0 0 100 72 82 601.0 0 82 73 672 601.0 0 672 74 589 601.0 0 589 75 303 601.0 0 303 76 138 601.0 0 138 77 1539 601.0 0 1539 78 631 601.0 0 631 79 375 601.0 0 375 80 67 601.0 0 67 81 60 601.0 0 60 82 62 601.0 0 62 83 45 601.0 0 45 84 56 601.0 0 56 85 69 601.0 0 69 86 52 601.0 0 52 87 51 601.0 0 51 88 64 601.0 0 64 89 78 601.0 0 78 90 49 601.0 0 49 91 58 601.0 0 58 92 81 601.0 0 81 93 67 601.0 0 67 94 66 601.0 0 66 95 64 601.0 0 64 96 74 601.0 0 74 97 71 601.0 0 71 98 69 601.0 0 69 99 71 601.0 0 71 100 77 601.0 0 77 101 84 601.0 0 84 102 36 601.0 0 36 103 85 601.0 0 85 104 70 601.0 0 70 105 81 601.0 0 81 106 115 601.0 0 115 107 313 601.0 0 313 108 341 601.0 0 341 109 82 601.0 0 82 110 63 601.0 0 63 111 48 601.0 0 48 112 120 601.0 0 120 113 81 601.0 0 81 114 69 601.0 0 69 115 79 601.0 0 79 116 76 601.0 0 76 117 66 601.0 0 66 118 81 601.0 0 81 119 56 601.0 0 56 120 73 601.0 0 73 121 75 601.0 0 75 122 52 601.0 0 52 123 64 601.0 0 64 124 66 601.0 0 66 125 87 601.0 0 87 126 76 601.0 0 76 127 108 601.0 0 108 128 520 601.0 0 520 129 304 601.0 0 304 130 243 601.0 0 243 131 90 601.0 0 90 132 78 601.0 0 78 133 124 601.0 0 124 134 175 601.0 0 175 135 211 601.0 0 211 136 434 601.0 0 434 137 147 601.0 0 147 138 72 601.0 0 72 139 112 601.0 0 112 140 91 601.0 0 91 141 63 601.0 0 63 142 142 601.0 0 142 143 92 601.0 0 92 144 76 601.0 0 76 145 132 601.0 0 132 146 1718 601.0 0 1718 147 695 601.0 0 695 148 271 601.0 0 271 149 131 601.0 0 131 150 96 601.0 0 96 151 62 601.0 0 62 152 60 601.0 0 60 153 79 601.0 0 79 154 60 601.0 0 60 155 100 601.0 0 100 156 156 601.0 0 156 157 178 601.0 0 178 158 64 601.0 0 64 159 96 601.0 0 96 160 88 601.0 0 88 161 76 601.0 0 76 162 71 601.0 0 71 163 56 601.0 0 56 164 206 601.0 0 206 165 136 601.0 0 136 166 107 601.0 0 107 167 71 601.0 0 71 168 51 601.0 0 51 169 75 601.0 0 75 170 66 601.0 0 66 171 78 601.0 0 78 172 55 601.0 0 55 173 54 601.0 0 54 174 63 601.0 0 63 175 58 601.0 0 58 176 41 601.0 0 41 177 61 601.0 0 61 178 44 601.0 0 44 179 51 601.0 0 51 180 49 601.0 0 49 181 44 601.0 0 44 182 65 601.0 0 65 183 49 601.0 0 49 184 169 601.0 0 169 185 147 601.0 0 147 186 147 601.0 0 147 187 72 601.0 0 72 188 53 601.0 0 53 189 40 601.0 0 40 190 45 601.0 0 45 191 61 601.0 0 61 192 64 601.0 0 64 193 76 601.0 0 76 194 61 601.0 0 61 195 509 601.0 0 509 196 379 601.0 0 379 197 131 601.0 0 131 198 73 601.0 0 73 199 78 601.0 0 78 200 70 601.0 0 70 201 84 601.0 0 84 202 55 601.0 0 55 203 67 601.0 0 67 204 65 601.0 0 65 205 87 601.0 0 87 206 89 601.0 0 89 207 56 601.0 0 56 208 46 601.0 0 46 209 68 601.0 0 68 210 54 601.0 0 54 211 52 601.0 0 52 212 47 601.0 0 47 213 47 601.0 0 47 214 48 601.0 0 48 215 112 601.0 0 112 216 154 601.0 0 154 217 150 601.0 0 150 218 87 601.0 0 87 219 97 601.0 0 97 220 79 601.0 0 79 221 47 601.0 0 47 222 59 601.0 0 59 223 55 601.0 0 55 224 61 601.0 0 61 225 105 601.0 0 105 226 69 601.0 0 69 227 59 601.0 0 59 228 59 601.0 0 59 229 67 601.0 0 67 230 48 601.0 0 48 231 87 601.0 0 87 232 83 601.0 0 83 233 83 601.0 0 83 234 123 601.0 0 123 235 191 601.0 0 191 236 1375 601.0 0 1375 237 2192 601.0 0 2192 238 3556 601.0 0 3556 239 89 601.0 0 89 240 67 601.0 0 67 241 66 601.0 0 66 242 52 601.0 0 52 243 55 601.0 0 55 244 60 601.0 0 60 245 71 601.0 0 71 246 206 601.0 0 206 247 233 601.0 0 233 248 244 601.0 0 244 249 95 601.0 0 95 250 234 601.0 0 234 251 118 601.0 0 118 252 160 601.0 0 160 253 128 601.0 0 128 254 81 601.0 0 81 255 51 601.0 0 51 256 51 601.0 0 51 257 59 601.0 0 59 258 41 601.0 0 41 259 112 601.0 0 112 260 36 601.0 0 36 261 48 601.0 0 48 262 48 601.0 0 48 263 74 601.0 0 74 264 53 601.0 0 53 265 45 601.0 0 45 266 56 601.0 0 56 267 42 601.0 0 42 268 61 601.0 0 61 269 64 601.0 0 64 270 70 601.0 0 70 271 52 601.0 0 52 272 58 601.0 0 58 273 87 601.0 0 87 274 71 601.0 0 71 275 47 601.0 0 47 276 113 601.0 0 113 277 93 601.0 0 93 278 128 601.0 0 128 279 81 601.0 0 81 280 150 601.0 0 150 281 338 601.0 0 338 282 244 601.0 0 244 283 130 601.0 0 130 284 189 601.0 0 189 285 129 601.0 0 129 286 74 601.0 0 74 287 79 601.0 0 79 288 61 601.0 0 61 289 70 601.0 0 70 290 71 601.0 0 71 291 78 601.0 0 78 292 83 601.0 0 83 293 85 601.0 0 85 294 109 601.0 0 109 295 114 601.0 0 114 296 263 601.0 0 263 297 340 601.0 0 340 298 410 601.0 0 410 299 261 601.0 0 261 300 202 601.0 0 202 301 208 601.0 0 208 Finished in 1.35 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 27,503 Reads with adapters: 2,269 (8.3%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 2,256 (8.2%) Total basepairs processed: 5,615,023 bp Total written (filtered): 607,317 bp (10.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2269 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 1 19 47 0.0 3 0 47 20 1943 0.0 4 1912 27 4 21 4 0.0 4 0 4 22 1 0.0 4 0 1 23 179 0.0 4 178 1 24 1 0.0 4 0 1 56 1 0.0 4 0 0 0 0 1 57 13 0.0 4 0 0 0 0 13 61 6 0.0 4 0 0 0 0 6 62 2 0.0 4 1 0 0 0 1 96 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 127 56 0.0 4 0 0 0 0 56 128 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 169 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 2,256 Reads with adapters: 196 (8.7%) Reads written (passing filters): 2,256 (100.0%) Total basepairs processed: 607,317 bp Total written (filtered): 605,270 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 196 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 4.1% G: 92.3% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 35.2 0 3 4 2 8.8 0 2 9 4 0.0 0 1 3 10 63 0.0 1 3 60 11 124 0.0 1 0 124 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.