This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.76 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 67,160 (2.7%) Reads that were too short: 31,551 (1.3%) Reads written (passing filters): 35,609 (1.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,798,623 bp (1.2%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 67160 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6702 601.0 0 6702 7 7506 601.0 0 7506 8 136 601.0 0 136 9 7834 601.0 0 7834 10 58 601.0 0 58 11 10 601.0 0 10 12 87 601.0 0 87 13 16 601.0 0 16 14 17 601.0 0 17 15 13 601.0 0 13 16 14 601.0 0 14 17 11 601.0 0 11 18 17 601.0 0 17 19 21 601.0 0 21 20 16 601.0 0 16 21 14 601.0 0 14 22 11 601.0 0 11 23 16 601.0 0 16 24 10 601.0 0 10 25 11 601.0 0 11 26 15 601.0 0 15 27 12 601.0 0 12 28 15 601.0 0 15 29 18 601.0 0 18 30 19 601.0 0 19 31 22 601.0 0 22 32 14 601.0 0 14 33 20 601.0 0 20 34 31 601.0 0 31 35 18 601.0 0 18 36 25 601.0 0 25 37 22 601.0 0 22 38 24 601.0 0 24 39 26 601.0 0 26 40 17 601.0 0 17 41 18 601.0 0 18 42 29 601.0 0 29 43 21 601.0 0 21 44 19 601.0 0 19 45 19 601.0 0 19 46 22 601.0 0 22 47 16 601.0 0 16 48 18 601.0 0 18 49 20 601.0 0 20 50 19 601.0 0 19 51 12 601.0 0 12 52 9 601.0 0 9 53 23 601.0 0 23 54 14 601.0 0 14 55 200 601.0 0 200 56 52 601.0 0 52 57 23 601.0 0 23 58 8 601.0 0 8 59 21 601.0 0 21 60 15 601.0 0 15 61 21 601.0 0 21 62 10 601.0 0 10 63 19 601.0 0 19 64 20 601.0 0 20 65 17 601.0 0 17 66 25 601.0 0 25 67 27 601.0 0 27 68 20 601.0 0 20 69 18 601.0 0 18 70 44 601.0 0 44 71 33 601.0 0 33 72 80 601.0 0 80 73 16 601.0 0 16 74 26 601.0 0 26 75 16 601.0 0 16 76 13 601.0 0 13 77 33 601.0 0 33 78 29 601.0 0 29 79 35 601.0 0 35 80 49 601.0 0 49 81 168 601.0 0 168 82 236 601.0 0 236 83 323 601.0 0 323 84 23 601.0 0 23 85 19 601.0 0 19 86 17 601.0 0 17 87 35 601.0 0 35 88 19 601.0 0 19 89 17 601.0 0 17 90 22 601.0 0 22 91 24 601.0 0 24 92 17 601.0 0 17 93 21 601.0 0 21 94 30 601.0 0 30 95 27 601.0 0 27 96 23 601.0 0 23 97 35 601.0 0 35 98 32 601.0 0 32 99 29 601.0 0 29 100 31 601.0 0 31 101 146 601.0 0 146 102 288 601.0 0 288 103 139 601.0 0 139 104 85 601.0 0 85 105 37 601.0 0 37 106 102 601.0 0 102 107 69 601.0 0 69 108 338 601.0 0 338 109 71 601.0 0 71 110 54 601.0 0 54 111 80 601.0 0 80 112 126 601.0 0 126 113 33 601.0 0 33 114 38 601.0 0 38 115 50 601.0 0 50 116 41 601.0 0 41 117 35 601.0 0 35 118 106 601.0 0 106 119 43 601.0 0 43 120 45 601.0 0 45 121 46 601.0 0 46 122 68 601.0 0 68 123 65 601.0 0 65 124 84 601.0 0 84 125 61 601.0 0 61 126 75 601.0 0 75 127 99 601.0 0 99 128 30 601.0 0 30 129 56 601.0 0 56 130 38 601.0 0 38 131 98 601.0 0 98 132 117 601.0 0 117 133 527 601.0 0 527 134 197 601.0 0 197 135 106 601.0 0 106 136 128 601.0 0 128 137 87 601.0 0 87 138 172 601.0 0 172 139 126 601.0 0 126 140 157 601.0 0 157 141 97 601.0 0 97 142 88 601.0 0 88 143 36 601.0 0 36 144 61 601.0 0 61 145 61 601.0 0 61 146 179 601.0 0 179 147 162 601.0 0 162 148 377 601.0 0 377 149 52 601.0 0 52 150 38 601.0 0 38 151 54 601.0 0 54 152 40 601.0 0 40 153 72 601.0 0 72 154 75 601.0 0 75 155 97 601.0 0 97 156 193 601.0 0 193 157 129 601.0 0 129 158 233 601.0 0 233 159 85 601.0 0 85 160 76 601.0 0 76 161 77 601.0 0 77 162 65 601.0 0 65 163 55 601.0 0 55 164 64 601.0 0 64 165 85 601.0 0 85 166 92 601.0 0 92 167 792 601.0 0 792 168 65 601.0 0 65 169 196 601.0 0 196 170 37 601.0 0 37 171 62 601.0 0 62 172 71 601.0 0 71 173 73 601.0 0 73 174 54 601.0 0 54 175 44 601.0 0 44 176 42 601.0 0 42 177 40 601.0 0 40 178 44 601.0 0 44 179 72 601.0 0 72 180 258 601.0 0 258 181 312 601.0 0 312 182 248 601.0 0 248 183 62 601.0 0 62 184 66 601.0 0 66 185 66 601.0 0 66 186 48 601.0 0 48 187 48 601.0 0 48 188 51 601.0 0 51 189 57 601.0 0 57 190 70 601.0 0 70 191 33 601.0 0 33 192 42 601.0 0 42 193 47 601.0 0 47 194 57 601.0 0 57 195 62 601.0 0 62 196 48 601.0 0 48 197 46 601.0 0 46 198 83 601.0 0 83 199 87 601.0 0 87 200 146 601.0 0 146 201 79 601.0 0 79 202 35 601.0 0 35 203 57 601.0 0 57 204 70 601.0 0 70 205 95 601.0 0 95 206 131 601.0 0 131 207 144 601.0 0 144 208 159 601.0 0 159 209 220 601.0 0 220 210 245 601.0 0 245 211 1322 601.0 0 1322 212 3912 601.0 0 3912 213 9229 601.0 0 9229 214 2885 601.0 0 2885 215 206 601.0 0 206 216 48 601.0 0 48 217 47 601.0 0 47 218 81 601.0 0 81 219 101 601.0 0 101 220 76 601.0 0 76 221 74 601.0 0 74 222 53 601.0 0 53 223 196 601.0 0 196 224 450 601.0 0 450 225 363 601.0 0 363 226 215 601.0 0 215 227 53 601.0 0 53 228 63 601.0 0 63 229 68 601.0 0 68 230 82 601.0 0 82 231 40 601.0 0 40 232 41 601.0 0 41 233 62 601.0 0 62 234 59 601.0 0 59 235 50 601.0 0 50 236 43 601.0 0 43 237 42 601.0 0 42 238 70 601.0 0 70 239 75 601.0 0 75 240 69 601.0 0 69 241 84 601.0 0 84 242 70 601.0 0 70 243 74 601.0 0 74 244 445 601.0 0 445 245 77 601.0 0 77 246 100 601.0 0 100 247 58 601.0 0 58 248 46 601.0 0 46 249 44 601.0 0 44 250 35 601.0 0 35 251 58 601.0 0 58 252 80 601.0 0 80 253 77 601.0 0 77 254 89 601.0 0 89 255 107 601.0 0 107 256 142 601.0 0 142 257 142 601.0 0 142 258 146 601.0 0 146 259 84 601.0 0 84 260 95 601.0 0 95 261 76 601.0 0 76 262 71 601.0 0 71 263 68 601.0 0 68 264 74 601.0 0 74 265 76 601.0 0 76 266 143 601.0 0 143 267 191 601.0 0 191 268 198 601.0 0 198 269 110 601.0 0 110 270 252 601.0 0 252 271 316 601.0 0 316 272 279 601.0 0 279 273 208 601.0 0 208 274 135 601.0 0 135 275 113 601.0 0 113 276 128 601.0 0 128 277 146 601.0 0 146 278 156 601.0 0 156 279 229 601.0 0 229 280 123 601.0 0 123 281 80 601.0 0 80 282 64 601.0 0 64 283 94 601.0 0 94 284 105 601.0 0 105 285 64 601.0 0 64 286 116 601.0 0 116 287 138 601.0 0 138 288 165 601.0 0 165 289 126 601.0 0 126 290 88 601.0 0 88 291 119 601.0 0 119 292 660 601.0 0 660 293 1385 601.0 0 1385 294 799 601.0 0 799 295 242 601.0 0 242 296 80 601.0 0 80 297 76 601.0 0 76 298 85 601.0 0 85 299 107 601.0 0 107 300 100 601.0 0 100 301 482 601.0 0 482 Finished in 1.79 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 35,609 Reads with adapters: 8,195 (23.0%) Reads that were too short: 82 (0.2%) Reads written (passing filters): 8,113 (22.8%) Total basepairs processed: 8,798,623 bp Total written (filtered): 2,219,599 bp (25.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8195 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 5 4 16 110 0.0 3 0 103 3 4 17 7841 0.0 3 7088 619 112 22 18 26 0.0 3 2 17 2 5 19 39 0.0 3 7 0 16 16 20 10 0.0 3 0 0 0 10 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 33 8 0.0 3 0 0 0 8 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 55 5 0.0 3 0 0 0 5 57 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 59 8 0.0 3 0 0 0 8 60 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 3 0.0 3 0 2 0 1 65 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 71 4 0.0 3 0 0 0 4 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 93 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 5 0.0 3 0 0 0 5 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 8,113 Reads with adapters: 175 (2.2%) Reads written (passing filters): 8,113 (100.0%) Total basepairs processed: 2,219,599 bp Total written (filtered): 2,219,054 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 175 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.0% C: 12.0% G: 17.7% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 162 126.8 0 162 4 11 31.7 0 11 6 1 2.0 0 1 9 1 0.0 0 0 1