This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.80 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 135,410 (5.5%) Reads that were too short: 98,165 (4.0%) Reads written (passing filters): 37,245 (1.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 6,453,404 bp (0.9%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 135410 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 159 601.0 0 159 7 150 601.0 0 150 8 2075 601.0 0 2075 9 1814 601.0 0 1814 10 118 601.0 0 118 11 208 601.0 0 208 12 55 601.0 0 55 13 63 601.0 0 63 14 100 601.0 0 100 15 68 601.0 0 68 16 63 601.0 0 63 17 78 601.0 0 78 18 67 601.0 0 67 19 60 601.0 0 60 20 60 601.0 0 60 21 66 601.0 0 66 22 53 601.0 0 53 23 41 601.0 0 41 24 65 601.0 0 65 25 39 601.0 0 39 26 76 601.0 0 76 27 51 601.0 0 51 28 52 601.0 0 52 29 44 601.0 0 44 30 63 601.0 0 63 31 59 601.0 0 59 32 43 601.0 0 43 33 54 601.0 0 54 34 48 601.0 0 48 35 52 601.0 0 52 36 35 601.0 0 35 37 72 601.0 0 72 38 102 601.0 0 102 39 52 601.0 0 52 40 59 601.0 0 59 41 68 601.0 0 68 42 47 601.0 0 47 43 41 601.0 0 41 44 52 601.0 0 52 45 47 601.0 0 47 46 83 601.0 0 83 47 99 601.0 0 99 48 119 601.0 0 119 49 63 601.0 0 63 50 62 601.0 0 62 51 50 601.0 0 50 52 60 601.0 0 60 53 79 601.0 0 79 54 40 601.0 0 40 55 55 601.0 0 55 56 46 601.0 0 46 57 72 601.0 0 72 58 85 601.0 0 85 59 52 601.0 0 52 60 84 601.0 0 84 61 39 601.0 0 39 62 71 601.0 0 71 63 34 601.0 0 34 64 67 601.0 0 67 65 40 601.0 0 40 66 55 601.0 0 55 67 187 601.0 0 187 68 157 601.0 0 157 69 141 601.0 0 141 70 52 601.0 0 52 71 75 601.0 0 75 72 63 601.0 0 63 73 59 601.0 0 59 74 73 601.0 0 73 75 108 601.0 0 108 76 54 601.0 0 54 77 79 601.0 0 79 78 120 601.0 0 120 79 90 601.0 0 90 80 60 601.0 0 60 81 67 601.0 0 67 82 69 601.0 0 69 83 52 601.0 0 52 84 53 601.0 0 53 85 62 601.0 0 62 86 78 601.0 0 78 87 52 601.0 0 52 88 61 601.0 0 61 89 53 601.0 0 53 90 41 601.0 0 41 91 58 601.0 0 58 92 51 601.0 0 51 93 66 601.0 0 66 94 55 601.0 0 55 95 103 601.0 0 103 96 67 601.0 0 67 97 318 601.0 0 318 98 122 601.0 0 122 99 124 601.0 0 124 100 57 601.0 0 57 101 69 601.0 0 69 102 46 601.0 0 46 103 77 601.0 0 77 104 64 601.0 0 64 105 69 601.0 0 69 106 136 601.0 0 136 107 136 601.0 0 136 108 90 601.0 0 90 109 97 601.0 0 97 110 66 601.0 0 66 111 78 601.0 0 78 112 91 601.0 0 91 113 77 601.0 0 77 114 91 601.0 0 91 115 87 601.0 0 87 116 90 601.0 0 90 117 90 601.0 0 90 118 89 601.0 0 89 119 168 601.0 0 168 120 117 601.0 0 117 121 102 601.0 0 102 122 82 601.0 0 82 123 112 601.0 0 112 124 114 601.0 0 114 125 668 601.0 0 668 126 254 601.0 0 254 127 493 601.0 0 493 128 121 601.0 0 121 129 117 601.0 0 117 130 89 601.0 0 89 131 85 601.0 0 85 132 86 601.0 0 86 133 142 601.0 0 142 134 199 601.0 0 199 135 502 601.0 0 502 136 196 601.0 0 196 137 101 601.0 0 101 138 145 601.0 0 145 139 130 601.0 0 130 140 73 601.0 0 73 141 106 601.0 0 106 142 98 601.0 0 98 143 99 601.0 0 99 144 92 601.0 0 92 145 738 601.0 0 738 146 453 601.0 0 453 147 398 601.0 0 398 148 157 601.0 0 157 149 90 601.0 0 90 150 101 601.0 0 101 151 86 601.0 0 86 152 91 601.0 0 91 153 86 601.0 0 86 154 136 601.0 0 136 155 145 601.0 0 145 156 133 601.0 0 133 157 125 601.0 0 125 158 141 601.0 0 141 159 221 601.0 0 221 160 118 601.0 0 118 161 117 601.0 0 117 162 112 601.0 0 112 163 145 601.0 0 145 164 131 601.0 0 131 165 188 601.0 0 188 166 255 601.0 0 255 167 305 601.0 0 305 168 124 601.0 0 124 169 115 601.0 0 115 170 202 601.0 0 202 171 136 601.0 0 136 172 173 601.0 0 173 173 512 601.0 0 512 174 1183 601.0 0 1183 175 2124 601.0 0 2124 176 1689 601.0 0 1689 177 109 601.0 0 109 178 122 601.0 0 122 179 136 601.0 0 136 180 211 601.0 0 211 181 595 601.0 0 595 182 552 601.0 0 552 183 606 601.0 0 606 184 282 601.0 0 282 185 263 601.0 0 263 186 2021 601.0 0 2021 187 1361 601.0 0 1361 188 1461 601.0 0 1461 189 246 601.0 0 246 190 133 601.0 0 133 191 135 601.0 0 135 192 218 601.0 0 218 193 199 601.0 0 199 194 153 601.0 0 153 195 168 601.0 0 168 196 106 601.0 0 106 197 119 601.0 0 119 198 73 601.0 0 73 199 126 601.0 0 126 200 113 601.0 0 113 201 172 601.0 0 172 202 107 601.0 0 107 203 158 601.0 0 158 204 152 601.0 0 152 205 158 601.0 0 158 206 827 601.0 0 827 207 791 601.0 0 791 208 643 601.0 0 643 209 156 601.0 0 156 210 167 601.0 0 167 211 320 601.0 0 320 212 470 601.0 0 470 213 204 601.0 0 204 214 304 601.0 0 304 215 309 601.0 0 309 216 198 601.0 0 198 217 205 601.0 0 205 218 259 601.0 0 259 219 309 601.0 0 309 220 360 601.0 0 360 221 375 601.0 0 375 222 454 601.0 0 454 223 412 601.0 0 412 224 818 601.0 0 818 225 15585 601.0 0 15585 226 7378 601.0 0 7378 227 5762 601.0 0 5762 228 223 601.0 0 223 229 355 601.0 0 355 230 3292 601.0 0 3292 231 3339 601.0 0 3339 232 1436 601.0 0 1436 233 415 601.0 0 415 234 280 601.0 0 280 235 270 601.0 0 270 236 349 601.0 0 349 237 244 601.0 0 244 238 198 601.0 0 198 239 134 601.0 0 134 240 145 601.0 0 145 241 219 601.0 0 219 242 201 601.0 0 201 243 152 601.0 0 152 244 183 601.0 0 183 245 183 601.0 0 183 246 461 601.0 0 461 247 495 601.0 0 495 248 235 601.0 0 235 249 177 601.0 0 177 250 885 601.0 0 885 251 636 601.0 0 636 252 1038 601.0 0 1038 253 1163 601.0 0 1163 254 2277 601.0 0 2277 255 1413 601.0 0 1413 256 90 601.0 0 90 257 97 601.0 0 97 258 117 601.0 0 117 259 146 601.0 0 146 260 103 601.0 0 103 261 132 601.0 0 132 262 272 601.0 0 272 263 256 601.0 0 256 264 251 601.0 0 251 265 261 601.0 0 261 266 155 601.0 0 155 267 177 601.0 0 177 268 256 601.0 0 256 269 280 601.0 0 280 270 191 601.0 0 191 271 478 601.0 0 478 272 306 601.0 0 306 273 253 601.0 0 253 274 233 601.0 0 233 275 478 601.0 0 478 276 361 601.0 0 361 277 344 601.0 0 344 278 448 601.0 0 448 279 1042 601.0 0 1042 280 3858 601.0 0 3858 281 5398 601.0 0 5398 282 6026 601.0 0 6026 283 2282 601.0 0 2282 284 395 601.0 0 395 285 648 601.0 0 648 286 303 601.0 0 303 287 283 601.0 0 283 288 238 601.0 0 238 289 305 601.0 0 305 290 436 601.0 0 436 291 275 601.0 0 275 292 256 601.0 0 256 293 171 601.0 0 171 294 396 601.0 0 396 295 510 601.0 0 510 296 682 601.0 0 682 297 904 601.0 0 904 298 1348 601.0 0 1348 299 1455 601.0 0 1455 300 2273 601.0 0 2273 301 5717 601.0 0 5717 Finished in 1.33 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 37,245 Reads with adapters: 2,419 (6.5%) Reads that were too short: 192 (0.5%) Reads written (passing filters): 2,227 (6.0%) Total basepairs processed: 6,453,404 bp Total written (filtered): 588,874 bp (9.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2419 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 26 0.0 3 0 25 1 17 2001 0.0 3 1832 139 24 6 18 13 0.0 3 1 4 3 5 19 13 0.0 3 3 2 6 2 20 16 0.0 3 3 1 1 11 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 0 2 26 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 11 0.0 3 0 0 0 11 37 6 0.0 3 0 0 0 6 38 2 0.0 3 0 0 0 2 39 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 52 0.0 3 0 0 0 52 47 3 0.0 3 0 0 0 3 48 2 0.0 3 0 0 0 2 49 9 0.0 3 0 0 1 8 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 37 0.0 3 0 0 0 37 69 3 0.0 3 0 0 1 2 71 3 0.0 3 0 0 0 3 72 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 1 3 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 5 0.0 3 0 0 0 5 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 18 0.0 3 0 0 1 17 98 3 0.0 3 0 0 0 3 99 3 0.0 3 0 0 0 3 100 24 0.0 3 0 0 2 22 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 105 4 0.0 3 0 0 0 4 106 7 0.0 3 0 0 0 7 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 7 0.0 3 0 0 0 7 114 2 0.0 3 0 0 1 1 115 11 0.0 3 0 0 1 10 116 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 1 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 124 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 1 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 149 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 3 0.0 3 0 0 0 3 159 2 0.0 3 0 0 1 1 161 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 1 0.0 3 1 184 1 0.0 3 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 1 187 3 0.0 3 0 0 0 3 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 231 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 2,227 Reads with adapters: 1 (0.0%) Reads written (passing filters): 2,227 (100.0%) Total basepairs processed: 588,874 bp Total written (filtered): 588,871 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 34.8 0 1