This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.53 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 128,325 (5.2%) Reads that were too short: 99,840 (4.1%) Reads written (passing filters): 28,485 (1.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 5,434,159 bp (0.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 128325 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 165 601.0 0 165 7 122 601.0 0 122 8 2124 601.0 0 2124 9 2059 601.0 0 2059 10 120 601.0 0 120 11 91 601.0 0 91 12 59 601.0 0 59 13 113 601.0 0 113 14 134 601.0 0 134 15 71 601.0 0 71 16 75 601.0 0 75 17 94 601.0 0 94 18 139 601.0 0 139 19 73 601.0 0 73 20 87 601.0 0 87 21 81 601.0 0 81 22 74 601.0 0 74 23 53 601.0 0 53 24 85 601.0 0 85 25 66 601.0 0 66 26 66 601.0 0 66 27 55 601.0 0 55 28 54 601.0 0 54 29 44 601.0 0 44 30 68 601.0 0 68 31 104 601.0 0 104 32 75 601.0 0 75 33 74 601.0 0 74 34 68 601.0 0 68 35 167 601.0 0 167 36 44 601.0 0 44 37 62 601.0 0 62 38 119 601.0 0 119 39 65 601.0 0 65 40 56 601.0 0 56 41 66 601.0 0 66 42 47 601.0 0 47 43 62 601.0 0 62 44 78 601.0 0 78 45 78 601.0 0 78 46 73 601.0 0 73 47 89 601.0 0 89 48 218 601.0 0 218 49 78 601.0 0 78 50 96 601.0 0 96 51 71 601.0 0 71 52 81 601.0 0 81 53 77 601.0 0 77 54 39 601.0 0 39 55 53 601.0 0 53 56 42 601.0 0 42 57 70 601.0 0 70 58 85 601.0 0 85 59 50 601.0 0 50 60 75 601.0 0 75 61 67 601.0 0 67 62 64 601.0 0 64 63 42 601.0 0 42 64 55 601.0 0 55 65 51 601.0 0 51 66 70 601.0 0 70 67 197 601.0 0 197 68 70 601.0 0 70 69 61 601.0 0 61 70 50 601.0 0 50 71 92 601.0 0 92 72 78 601.0 0 78 73 50 601.0 0 50 74 57 601.0 0 57 75 110 601.0 0 110 76 57 601.0 0 57 77 82 601.0 0 82 78 109 601.0 0 109 79 67 601.0 0 67 80 53 601.0 0 53 81 66 601.0 0 66 82 75 601.0 0 75 83 61 601.0 0 61 84 61 601.0 0 61 85 62 601.0 0 62 86 59 601.0 0 59 87 57 601.0 0 57 88 73 601.0 0 73 89 66 601.0 0 66 90 61 601.0 0 61 91 60 601.0 0 60 92 67 601.0 0 67 93 63 601.0 0 63 94 63 601.0 0 63 95 81 601.0 0 81 96 67 601.0 0 67 97 166 601.0 0 166 98 104 601.0 0 104 99 91 601.0 0 91 100 52 601.0 0 52 101 59 601.0 0 59 102 54 601.0 0 54 103 59 601.0 0 59 104 64 601.0 0 64 105 73 601.0 0 73 106 124 601.0 0 124 107 87 601.0 0 87 108 63 601.0 0 63 109 121 601.0 0 121 110 66 601.0 0 66 111 76 601.0 0 76 112 90 601.0 0 90 113 79 601.0 0 79 114 69 601.0 0 69 115 64 601.0 0 64 116 104 601.0 0 104 117 99 601.0 0 99 118 85 601.0 0 85 119 120 601.0 0 120 120 146 601.0 0 146 121 90 601.0 0 90 122 80 601.0 0 80 123 100 601.0 0 100 124 87 601.0 0 87 125 623 601.0 0 623 126 187 601.0 0 187 127 446 601.0 0 446 128 65 601.0 0 65 129 75 601.0 0 75 130 77 601.0 0 77 131 89 601.0 0 89 132 67 601.0 0 67 133 79 601.0 0 79 134 116 601.0 0 116 135 381 601.0 0 381 136 129 601.0 0 129 137 72 601.0 0 72 138 99 601.0 0 99 139 80 601.0 0 80 140 77 601.0 0 77 141 85 601.0 0 85 142 91 601.0 0 91 143 82 601.0 0 82 144 73 601.0 0 73 145 397 601.0 0 397 146 207 601.0 0 207 147 249 601.0 0 249 148 125 601.0 0 125 149 91 601.0 0 91 150 63 601.0 0 63 151 84 601.0 0 84 152 61 601.0 0 61 153 77 601.0 0 77 154 67 601.0 0 67 155 82 601.0 0 82 156 87 601.0 0 87 157 102 601.0 0 102 158 113 601.0 0 113 159 109 601.0 0 109 160 80 601.0 0 80 161 79 601.0 0 79 162 98 601.0 0 98 163 104 601.0 0 104 164 110 601.0 0 110 165 110 601.0 0 110 166 121 601.0 0 121 167 127 601.0 0 127 168 103 601.0 0 103 169 103 601.0 0 103 170 208 601.0 0 208 171 120 601.0 0 120 172 180 601.0 0 180 173 364 601.0 0 364 174 675 601.0 0 675 175 1128 601.0 0 1128 176 738 601.0 0 738 177 95 601.0 0 95 178 86 601.0 0 86 179 123 601.0 0 123 180 136 601.0 0 136 181 428 601.0 0 428 182 384 601.0 0 384 183 429 601.0 0 429 184 205 601.0 0 205 185 113 601.0 0 113 186 684 601.0 0 684 187 703 601.0 0 703 188 872 601.0 0 872 189 168 601.0 0 168 190 84 601.0 0 84 191 106 601.0 0 106 192 135 601.0 0 135 193 123 601.0 0 123 194 107 601.0 0 107 195 99 601.0 0 99 196 93 601.0 0 93 197 71 601.0 0 71 198 69 601.0 0 69 199 83 601.0 0 83 200 82 601.0 0 82 201 122 601.0 0 122 202 106 601.0 0 106 203 140 601.0 0 140 204 84 601.0 0 84 205 91 601.0 0 91 206 231 601.0 0 231 207 367 601.0 0 367 208 195 601.0 0 195 209 133 601.0 0 133 210 139 601.0 0 139 211 324 601.0 0 324 212 517 601.0 0 517 213 157 601.0 0 157 214 287 601.0 0 287 215 334 601.0 0 334 216 188 601.0 0 188 217 162 601.0 0 162 218 262 601.0 0 262 219 231 601.0 0 231 220 242 601.0 0 242 221 243 601.0 0 243 222 318 601.0 0 318 223 305 601.0 0 305 224 679 601.0 0 679 225 18419 601.0 0 18419 226 8260 601.0 0 8260 227 6394 601.0 0 6394 228 115 601.0 0 115 229 316 601.0 0 316 230 1366 601.0 0 1366 231 1757 601.0 0 1757 232 515 601.0 0 515 233 174 601.0 0 174 234 152 601.0 0 152 235 130 601.0 0 130 236 136 601.0 0 136 237 139 601.0 0 139 238 118 601.0 0 118 239 82 601.0 0 82 240 92 601.0 0 92 241 146 601.0 0 146 242 117 601.0 0 117 243 124 601.0 0 124 244 175 601.0 0 175 245 154 601.0 0 154 246 286 601.0 0 286 247 346 601.0 0 346 248 165 601.0 0 165 249 158 601.0 0 158 250 709 601.0 0 709 251 420 601.0 0 420 252 653 601.0 0 653 253 505 601.0 0 505 254 1045 601.0 0 1045 255 530 601.0 0 530 256 79 601.0 0 79 257 99 601.0 0 99 258 131 601.0 0 131 259 128 601.0 0 128 260 75 601.0 0 75 261 88 601.0 0 88 262 118 601.0 0 118 263 136 601.0 0 136 264 177 601.0 0 177 265 477 601.0 0 477 266 201 601.0 0 201 267 193 601.0 0 193 268 207 601.0 0 207 269 196 601.0 0 196 270 163 601.0 0 163 271 342 601.0 0 342 272 330 601.0 0 330 273 224 601.0 0 224 274 335 601.0 0 335 275 944 601.0 0 944 276 459 601.0 0 459 277 158 601.0 0 158 278 207 601.0 0 207 279 355 601.0 0 355 280 1349 601.0 0 1349 281 1515 601.0 0 1515 282 1601 601.0 0 1601 283 906 601.0 0 906 284 684 601.0 0 684 285 1391 601.0 0 1391 286 681 601.0 0 681 287 512 601.0 0 512 288 390 601.0 0 390 289 441 601.0 0 441 290 735 601.0 0 735 291 531 601.0 0 531 292 449 601.0 0 449 293 370 601.0 0 370 294 506 601.0 0 506 295 731 601.0 0 731 296 1201 601.0 0 1201 297 1833 601.0 0 1833 298 2927 601.0 0 2927 299 3682 601.0 0 3682 300 5780 601.0 0 5780 301 16370 601.0 0 16370 Finished in 1.20 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 28,485 Reads with adapters: 2,093 (7.3%) Reads that were too short: 17 (0.1%) Reads written (passing filters): 2,076 (7.3%) Total basepairs processed: 5,434,159 bp Total written (filtered): 561,851 bp (10.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2093 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 0 7 18 1 0.0 3 0 0 1 19 25 0.0 3 0 25 20 2003 0.0 4 1973 29 1 21 6 0.0 4 1 5 22 5 0.0 4 4 0 0 0 1 23 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 40 1 0.0 4 1 42 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 1 84 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 22 0.0 4 0 0 0 0 22 144 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 156 1 0.0 4 1 187 1 0.0 4 0 0 0 1 188 2 0.0 4 1 0 0 1 189 1 0.0 4 0 0 1 209 1 0.0 4 0 0 1 219 1 0.0 4 0 0 0 0 1 225 1 0.0 4 1 239 2 0.0 4 0 0 0 0 2 241 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (39 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 2,076 Reads with adapters: 327 (15.8%) Reads written (passing filters): 2,076 (100.0%) Total basepairs processed: 561,851 bp Total written (filtered): 557,514 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 327 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.1% C: 1.8% G: 85.3% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 159 0.0 0 8 151 10 95 0.0 1 3 92 11 35 0.0 1 0 35 18 11 0.0 1 9 2 22 7 0.0 1 5 2 23 11 0.0 1 11 25 1 0.0 1 1 105 2 0.0 1 1 1 106 2 0.0 1 1 1 107 1 0.0 1 1 136 1 0.0 1 0 1 138 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.