This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.76 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 118,429 (4.8%) Reads that were too short: 20,745 (0.8%) Reads written (passing filters): 97,684 (4.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 17,287,601 bp (2.3%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 118429 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 209 601.0 0 209 7 257 601.0 0 257 8 199 601.0 0 199 9 301 601.0 0 301 10 192 601.0 0 192 11 178 601.0 0 178 12 162 601.0 0 162 13 172 601.0 0 172 14 152 601.0 0 152 15 185 601.0 0 185 16 166 601.0 0 166 17 229 601.0 0 229 18 181 601.0 0 181 19 131 601.0 0 131 20 154 601.0 0 154 21 248 601.0 0 248 22 225 601.0 0 225 23 253 601.0 0 253 24 1690 601.0 0 1690 25 432 601.0 0 432 26 399 601.0 0 399 27 135 601.0 0 135 28 170 601.0 0 170 29 160 601.0 0 160 30 163 601.0 0 163 31 150 601.0 0 150 32 204 601.0 0 204 33 179 601.0 0 179 34 124 601.0 0 124 35 167 601.0 0 167 36 191 601.0 0 191 37 148 601.0 0 148 38 127 601.0 0 127 39 153 601.0 0 153 40 160 601.0 0 160 41 178 601.0 0 178 42 189 601.0 0 189 43 164 601.0 0 164 44 158 601.0 0 158 45 130 601.0 0 130 46 156 601.0 0 156 47 169 601.0 0 169 48 163 601.0 0 163 49 186 601.0 0 186 50 151 601.0 0 151 51 165 601.0 0 165 52 140 601.0 0 140 53 193 601.0 0 193 54 147 601.0 0 147 55 160 601.0 0 160 56 154 601.0 0 154 57 171 601.0 0 171 58 145 601.0 0 145 59 190 601.0 0 190 60 158 601.0 0 158 61 473 601.0 0 473 62 336 601.0 0 336 63 227 601.0 0 227 64 137 601.0 0 137 65 128 601.0 0 128 66 166 601.0 0 166 67 177 601.0 0 177 68 166 601.0 0 166 69 229 601.0 0 229 70 219 601.0 0 219 71 203 601.0 0 203 72 179 601.0 0 179 73 177 601.0 0 177 74 220 601.0 0 220 75 194 601.0 0 194 76 142 601.0 0 142 77 161 601.0 0 161 78 208 601.0 0 208 79 192 601.0 0 192 80 187 601.0 0 187 81 194 601.0 0 194 82 201 601.0 0 201 83 816 601.0 0 816 84 1004 601.0 0 1004 85 488 601.0 0 488 86 176 601.0 0 176 87 157 601.0 0 157 88 215 601.0 0 215 89 150 601.0 0 150 90 164 601.0 0 164 91 172 601.0 0 172 92 171 601.0 0 171 93 167 601.0 0 167 94 149 601.0 0 149 95 152 601.0 0 152 96 172 601.0 0 172 97 189 601.0 0 189 98 231 601.0 0 231 99 341 601.0 0 341 100 172 601.0 0 172 101 261 601.0 0 261 102 271 601.0 0 271 103 188 601.0 0 188 104 169 601.0 0 169 105 245 601.0 0 245 106 218 601.0 0 218 107 225 601.0 0 225 108 257 601.0 0 257 109 247 601.0 0 247 110 310 601.0 0 310 111 447 601.0 0 447 112 7775 601.0 0 7775 113 7532 601.0 0 7532 114 4315 601.0 0 4315 115 189 601.0 0 189 116 130 601.0 0 130 117 146 601.0 0 146 118 204 601.0 0 204 119 227 601.0 0 227 120 1531 601.0 0 1531 121 778 601.0 0 778 122 667 601.0 0 667 123 309 601.0 0 309 124 398 601.0 0 398 125 4282 601.0 0 4282 126 6110 601.0 0 6110 127 1840 601.0 0 1840 128 179 601.0 0 179 129 273 601.0 0 273 130 167 601.0 0 167 131 194 601.0 0 194 132 173 601.0 0 173 133 180 601.0 0 180 134 181 601.0 0 181 135 162 601.0 0 162 136 277 601.0 0 277 137 209 601.0 0 209 138 216 601.0 0 216 139 204 601.0 0 204 140 200 601.0 0 200 141 185 601.0 0 185 142 393 601.0 0 393 143 347 601.0 0 347 144 263 601.0 0 263 145 204 601.0 0 204 146 240 601.0 0 240 147 1185 601.0 0 1185 148 1283 601.0 0 1283 149 816 601.0 0 816 150 438 601.0 0 438 151 210 601.0 0 210 152 279 601.0 0 279 153 320 601.0 0 320 154 182 601.0 0 182 155 174 601.0 0 174 156 246 601.0 0 246 157 222 601.0 0 222 158 202 601.0 0 202 159 195 601.0 0 195 160 192 601.0 0 192 161 198 601.0 0 198 162 216 601.0 0 216 163 240 601.0 0 240 164 211 601.0 0 211 165 301 601.0 0 301 166 771 601.0 0 771 167 652 601.0 0 652 168 614 601.0 0 614 169 371 601.0 0 371 170 319 601.0 0 319 171 1525 601.0 0 1525 172 1415 601.0 0 1415 173 1248 601.0 0 1248 174 281 601.0 0 281 175 269 601.0 0 269 176 187 601.0 0 187 177 239 601.0 0 239 178 230 601.0 0 230 179 181 601.0 0 181 180 225 601.0 0 225 181 275 601.0 0 275 182 364 601.0 0 364 183 2430 601.0 0 2430 184 2336 601.0 0 2336 185 4729 601.0 0 4729 186 280 601.0 0 280 187 305 601.0 0 305 188 284 601.0 0 284 189 225 601.0 0 225 190 231 601.0 0 231 191 1108 601.0 0 1108 192 1315 601.0 0 1315 193 1258 601.0 0 1258 194 434 601.0 0 434 195 225 601.0 0 225 196 213 601.0 0 213 197 179 601.0 0 179 198 175 601.0 0 175 199 355 601.0 0 355 200 304 601.0 0 304 201 289 601.0 0 289 202 373 601.0 0 373 203 198 601.0 0 198 204 156 601.0 0 156 205 174 601.0 0 174 206 149 601.0 0 149 207 195 601.0 0 195 208 184 601.0 0 184 209 179 601.0 0 179 210 167 601.0 0 167 211 151 601.0 0 151 212 147 601.0 0 147 213 167 601.0 0 167 214 147 601.0 0 147 215 222 601.0 0 222 216 261 601.0 0 261 217 300 601.0 0 300 218 208 601.0 0 208 219 208 601.0 0 208 220 176 601.0 0 176 221 129 601.0 0 129 222 163 601.0 0 163 223 120 601.0 0 120 224 151 601.0 0 151 225 209 601.0 0 209 226 391 601.0 0 391 227 303 601.0 0 303 228 358 601.0 0 358 229 308 601.0 0 308 230 580 601.0 0 580 231 157 601.0 0 157 232 165 601.0 0 165 233 141 601.0 0 141 234 144 601.0 0 144 235 157 601.0 0 157 236 130 601.0 0 130 237 185 601.0 0 185 238 158 601.0 0 158 239 143 601.0 0 143 240 150 601.0 0 150 241 143 601.0 0 143 242 143 601.0 0 143 243 175 601.0 0 175 244 165 601.0 0 165 245 208 601.0 0 208 246 184 601.0 0 184 247 240 601.0 0 240 248 148 601.0 0 148 249 144 601.0 0 144 250 122 601.0 0 122 251 158 601.0 0 158 252 177 601.0 0 177 253 149 601.0 0 149 254 155 601.0 0 155 255 178 601.0 0 178 256 175 601.0 0 175 257 175 601.0 0 175 258 134 601.0 0 134 259 133 601.0 0 133 260 174 601.0 0 174 261 150 601.0 0 150 262 154 601.0 0 154 263 146 601.0 0 146 264 178 601.0 0 178 265 148 601.0 0 148 266 188 601.0 0 188 267 1503 601.0 0 1503 268 1571 601.0 0 1571 269 309 601.0 0 309 270 153 601.0 0 153 271 155 601.0 0 155 272 144 601.0 0 144 273 115 601.0 0 115 274 122 601.0 0 122 275 199 601.0 0 199 276 147 601.0 0 147 277 440 601.0 0 440 278 204 601.0 0 204 279 154 601.0 0 154 280 157 601.0 0 157 281 150 601.0 0 150 282 134 601.0 0 134 283 175 601.0 0 175 284 139 601.0 0 139 285 122 601.0 0 122 286 138 601.0 0 138 287 146 601.0 0 146 288 137 601.0 0 137 289 143 601.0 0 143 290 141 601.0 0 141 291 138 601.0 0 138 292 140 601.0 0 140 293 162 601.0 0 162 294 147 601.0 0 147 295 161 601.0 0 161 296 128 601.0 0 128 297 140 601.0 0 140 298 209 601.0 0 209 299 217 601.0 0 217 300 129 601.0 0 129 301 128 601.0 0 128 Finished in 3.84 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 97,684 Reads with adapters: 205 (0.2%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 173 (0.2%) Total basepairs processed: 17,287,601 bp Total written (filtered): 41,609 bp (0.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 205 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 20 107 0.0 4 103 4 22 13 0.0 4 11 0 1 0 1 23 1 0.0 4 0 0 0 0 1 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 2 0.0 4 0 0 0 0 2 51 34 0.0 4 0 0 0 0 34 59 2 0.0 4 0 0 0 0 2 73 1 0.0 4 0 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 1 79 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 1 110 9 0.0 4 0 0 0 0 9 113 2 0.0 4 0 0 0 0 2 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 1 0 0 0 1 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 172 1 0.0 4 1 185 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 173 Reads with adapters: 8 (4.6%) Reads written (passing filters): 173 (100.0%) Total basepairs processed: 41,609 bp Total written (filtered): 41,553 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 37.5% C: 12.5% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 2.7 0 3 4 1 0.7 0 1 10 1 0.0 1 0 1 11 3 0.0 1 0 3