This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.58 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 94,466 (3.8%) Reads that were too short: 47,048 (1.9%) Reads written (passing filters): 47,418 (1.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,984,969 bp (1.5%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 94466 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 220 601.0 0 220 7 383 601.0 0 383 8 10963 601.0 0 10963 9 8042 601.0 0 8042 10 111 601.0 0 111 11 99 601.0 0 99 12 115 601.0 0 115 13 112 601.0 0 112 14 143 601.0 0 143 15 121 601.0 0 121 16 135 601.0 0 135 17 110 601.0 0 110 18 103 601.0 0 103 19 128 601.0 0 128 20 106 601.0 0 106 21 102 601.0 0 102 22 110 601.0 0 110 23 120 601.0 0 120 24 97 601.0 0 97 25 127 601.0 0 127 26 132 601.0 0 132 27 123 601.0 0 123 28 111 601.0 0 111 29 145 601.0 0 145 30 107 601.0 0 107 31 100 601.0 0 100 32 120 601.0 0 120 33 90 601.0 0 90 34 113 601.0 0 113 35 100 601.0 0 100 36 95 601.0 0 95 37 116 601.0 0 116 38 103 601.0 0 103 39 96 601.0 0 96 40 121 601.0 0 121 41 95 601.0 0 95 42 108 601.0 0 108 43 115 601.0 0 115 44 171 601.0 0 171 45 153 601.0 0 153 46 120 601.0 0 120 47 127 601.0 0 127 48 123 601.0 0 123 49 102 601.0 0 102 50 105 601.0 0 105 51 105 601.0 0 105 52 108 601.0 0 108 53 145 601.0 0 145 54 165 601.0 0 165 55 123 601.0 0 123 56 170 601.0 0 170 57 102 601.0 0 102 58 185 601.0 0 185 59 220 601.0 0 220 60 212 601.0 0 212 61 202 601.0 0 202 62 120 601.0 0 120 63 96 601.0 0 96 64 105 601.0 0 105 65 120 601.0 0 120 66 104 601.0 0 104 67 156 601.0 0 156 68 108 601.0 0 108 69 121 601.0 0 121 70 147 601.0 0 147 71 259 601.0 0 259 72 149 601.0 0 149 73 155 601.0 0 155 74 111 601.0 0 111 75 127 601.0 0 127 76 224 601.0 0 224 77 185 601.0 0 185 78 210 601.0 0 210 79 136 601.0 0 136 80 122 601.0 0 122 81 168 601.0 0 168 82 129 601.0 0 129 83 119 601.0 0 119 84 110 601.0 0 110 85 141 601.0 0 141 86 90 601.0 0 90 87 94 601.0 0 94 88 113 601.0 0 113 89 108 601.0 0 108 90 121 601.0 0 121 91 130 601.0 0 130 92 151 601.0 0 151 93 158 601.0 0 158 94 137 601.0 0 137 95 141 601.0 0 141 96 121 601.0 0 121 97 109 601.0 0 109 98 116 601.0 0 116 99 107 601.0 0 107 100 135 601.0 0 135 101 107 601.0 0 107 102 117 601.0 0 117 103 135 601.0 0 135 104 100 601.0 0 100 105 126 601.0 0 126 106 131 601.0 0 131 107 127 601.0 0 127 108 132 601.0 0 132 109 146 601.0 0 146 110 220 601.0 0 220 111 205 601.0 0 205 112 224 601.0 0 224 113 149 601.0 0 149 114 134 601.0 0 134 115 128 601.0 0 128 116 133 601.0 0 133 117 150 601.0 0 150 118 111 601.0 0 111 119 122 601.0 0 122 120 138 601.0 0 138 121 106 601.0 0 106 122 113 601.0 0 113 123 134 601.0 0 134 124 106 601.0 0 106 125 137 601.0 0 137 126 149 601.0 0 149 127 124 601.0 0 124 128 161 601.0 0 161 129 161 601.0 0 161 130 244 601.0 0 244 131 265 601.0 0 265 132 307 601.0 0 307 133 169 601.0 0 169 134 197 601.0 0 197 135 159 601.0 0 159 136 161 601.0 0 161 137 142 601.0 0 142 138 259 601.0 0 259 139 237 601.0 0 237 140 205 601.0 0 205 141 145 601.0 0 145 142 200 601.0 0 200 143 173 601.0 0 173 144 207 601.0 0 207 145 219 601.0 0 219 146 170 601.0 0 170 147 170 601.0 0 170 148 212 601.0 0 212 149 140 601.0 0 140 150 153 601.0 0 153 151 190 601.0 0 190 152 143 601.0 0 143 153 139 601.0 0 139 154 140 601.0 0 140 155 131 601.0 0 131 156 152 601.0 0 152 157 155 601.0 0 155 158 151 601.0 0 151 159 132 601.0 0 132 160 102 601.0 0 102 161 139 601.0 0 139 162 116 601.0 0 116 163 126 601.0 0 126 164 128 601.0 0 128 165 153 601.0 0 153 166 151 601.0 0 151 167 114 601.0 0 114 168 131 601.0 0 131 169 104 601.0 0 104 170 130 601.0 0 130 171 117 601.0 0 117 172 109 601.0 0 109 173 113 601.0 0 113 174 133 601.0 0 133 175 108 601.0 0 108 176 114 601.0 0 114 177 112 601.0 0 112 178 114 601.0 0 114 179 170 601.0 0 170 180 177 601.0 0 177 181 139 601.0 0 139 182 116 601.0 0 116 183 128 601.0 0 128 184 107 601.0 0 107 185 109 601.0 0 109 186 136 601.0 0 136 187 113 601.0 0 113 188 126 601.0 0 126 189 109 601.0 0 109 190 144 601.0 0 144 191 143 601.0 0 143 192 175 601.0 0 175 193 192 601.0 0 192 194 148 601.0 0 148 195 158 601.0 0 158 196 123 601.0 0 123 197 306 601.0 0 306 198 468 601.0 0 468 199 760 601.0 0 760 200 106 601.0 0 106 201 130 601.0 0 130 202 96 601.0 0 96 203 87 601.0 0 87 204 169 601.0 0 169 205 131 601.0 0 131 206 117 601.0 0 117 207 88 601.0 0 88 208 99 601.0 0 99 209 113 601.0 0 113 210 136 601.0 0 136 211 118 601.0 0 118 212 128 601.0 0 128 213 128 601.0 0 128 214 109 601.0 0 109 215 135 601.0 0 135 216 125 601.0 0 125 217 117 601.0 0 117 218 119 601.0 0 119 219 99 601.0 0 99 220 113 601.0 0 113 221 124 601.0 0 124 222 146 601.0 0 146 223 286 601.0 0 286 224 154 601.0 0 154 225 374 601.0 0 374 226 142 601.0 0 142 227 156 601.0 0 156 228 133 601.0 0 133 229 95 601.0 0 95 230 125 601.0 0 125 231 118 601.0 0 118 232 127 601.0 0 127 233 191 601.0 0 191 234 162 601.0 0 162 235 130 601.0 0 130 236 152 601.0 0 152 237 139 601.0 0 139 238 209 601.0 0 209 239 197 601.0 0 197 240 318 601.0 0 318 241 338 601.0 0 338 242 229 601.0 0 229 243 143 601.0 0 143 244 135 601.0 0 135 245 247 601.0 0 247 246 261 601.0 0 261 247 196 601.0 0 196 248 134 601.0 0 134 249 117 601.0 0 117 250 118 601.0 0 118 251 132 601.0 0 132 252 144 601.0 0 144 253 125 601.0 0 125 254 180 601.0 0 180 255 204 601.0 0 204 256 273 601.0 0 273 257 214 601.0 0 214 258 213 601.0 0 213 259 168 601.0 0 168 260 175 601.0 0 175 261 211 601.0 0 211 262 257 601.0 0 257 263 225 601.0 0 225 264 212 601.0 0 212 265 1234 601.0 0 1234 266 1114 601.0 0 1114 267 1295 601.0 0 1295 268 310 601.0 0 310 269 110 601.0 0 110 270 105 601.0 0 105 271 166 601.0 0 166 272 250 601.0 0 250 273 255 601.0 0 255 274 182 601.0 0 182 275 146 601.0 0 146 276 124 601.0 0 124 277 153 601.0 0 153 278 172 601.0 0 172 279 196 601.0 0 196 280 204 601.0 0 204 281 223 601.0 0 223 282 197 601.0 0 197 283 176 601.0 0 176 284 239 601.0 0 239 285 245 601.0 0 245 286 294 601.0 0 294 287 254 601.0 0 254 288 343 601.0 0 343 289 268 601.0 0 268 290 268 601.0 0 268 291 392 601.0 0 392 292 425 601.0 0 425 293 428 601.0 0 428 294 396 601.0 0 396 295 390 601.0 0 390 296 440 601.0 0 440 297 1624 601.0 0 1624 298 6467 601.0 0 6467 299 7434 601.0 0 7434 300 6453 601.0 0 6453 301 3720 601.0 0 3720 Finished in 2.01 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 47,418 Reads with adapters: 10,742 (22.7%) Reads that were too short: 93 (0.2%) Reads written (passing filters): 10,649 (22.5%) Total basepairs processed: 10,984,969 bp Total written (filtered): 2,930,963 bp (26.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10742 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 9 0.0 3 0 0 4 5 16 98 0.0 3 0 76 16 6 17 10366 0.0 3 9520 688 130 28 18 51 0.0 3 3 31 7 10 19 37 0.0 3 0 0 21 16 20 29 0.0 3 0 0 1 28 26 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 35 1 0.0 3 1 39 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 4 0.0 3 0 0 1 3 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 1 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 5 0.0 3 0 0 0 5 119 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 0 4 128 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 1 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 189 41 0.0 3 0 0 0 41 194 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 1 216 1 0.0 3 0 0 1 217 4 0.0 3 0 0 1 3 221 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 10,649 Reads with adapters: 41 (0.4%) Reads written (passing filters): 10,649 (100.0%) Total basepairs processed: 2,930,963 bp Total written (filtered): 2,930,826 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 41 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.0% C: 17.1% G: 26.8% T: 17.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 31 166.4 0 31 4 6 41.6 0 6 5 4 10.4 0 4