This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.01 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 104,233 (4.2%) Reads that were too short: 61,498 (2.5%) Reads written (passing filters): 42,735 (1.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,246,693 bp (1.4%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 104233 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 158 601.0 0 158 7 312 601.0 0 312 8 9575 601.0 0 9575 9 10849 601.0 0 10849 10 66 601.0 0 66 11 73 601.0 0 73 12 105 601.0 0 105 13 84 601.0 0 84 14 92 601.0 0 92 15 108 601.0 0 108 16 112 601.0 0 112 17 84 601.0 0 84 18 97 601.0 0 97 19 90 601.0 0 90 20 87 601.0 0 87 21 98 601.0 0 98 22 91 601.0 0 91 23 106 601.0 0 106 24 109 601.0 0 109 25 99 601.0 0 99 26 93 601.0 0 93 27 90 601.0 0 90 28 74 601.0 0 74 29 100 601.0 0 100 30 72 601.0 0 72 31 67 601.0 0 67 32 99 601.0 0 99 33 75 601.0 0 75 34 104 601.0 0 104 35 81 601.0 0 81 36 75 601.0 0 75 37 101 601.0 0 101 38 83 601.0 0 83 39 69 601.0 0 69 40 92 601.0 0 92 41 96 601.0 0 96 42 102 601.0 0 102 43 78 601.0 0 78 44 115 601.0 0 115 45 108 601.0 0 108 46 100 601.0 0 100 47 80 601.0 0 80 48 115 601.0 0 115 49 88 601.0 0 88 50 87 601.0 0 87 51 80 601.0 0 80 52 110 601.0 0 110 53 83 601.0 0 83 54 122 601.0 0 122 55 103 601.0 0 103 56 130 601.0 0 130 57 72 601.0 0 72 58 187 601.0 0 187 59 200 601.0 0 200 60 235 601.0 0 235 61 233 601.0 0 233 62 88 601.0 0 88 63 103 601.0 0 103 64 104 601.0 0 104 65 99 601.0 0 99 66 84 601.0 0 84 67 107 601.0 0 107 68 105 601.0 0 105 69 111 601.0 0 111 70 130 601.0 0 130 71 360 601.0 0 360 72 125 601.0 0 125 73 111 601.0 0 111 74 83 601.0 0 83 75 94 601.0 0 94 76 115 601.0 0 115 77 114 601.0 0 114 78 148 601.0 0 148 79 101 601.0 0 101 80 115 601.0 0 115 81 151 601.0 0 151 82 102 601.0 0 102 83 90 601.0 0 90 84 97 601.0 0 97 85 106 601.0 0 106 86 87 601.0 0 87 87 112 601.0 0 112 88 78 601.0 0 78 89 105 601.0 0 105 90 105 601.0 0 105 91 133 601.0 0 133 92 109 601.0 0 109 93 107 601.0 0 107 94 105 601.0 0 105 95 89 601.0 0 89 96 87 601.0 0 87 97 84 601.0 0 84 98 97 601.0 0 97 99 88 601.0 0 88 100 79 601.0 0 79 101 86 601.0 0 86 102 111 601.0 0 111 103 110 601.0 0 110 104 98 601.0 0 98 105 94 601.0 0 94 106 106 601.0 0 106 107 80 601.0 0 80 108 85 601.0 0 85 109 124 601.0 0 124 110 112 601.0 0 112 111 130 601.0 0 130 112 122 601.0 0 122 113 96 601.0 0 96 114 115 601.0 0 115 115 80 601.0 0 80 116 116 601.0 0 116 117 89 601.0 0 89 118 104 601.0 0 104 119 80 601.0 0 80 120 111 601.0 0 111 121 94 601.0 0 94 122 94 601.0 0 94 123 98 601.0 0 98 124 81 601.0 0 81 125 114 601.0 0 114 126 89 601.0 0 89 127 77 601.0 0 77 128 87 601.0 0 87 129 113 601.0 0 113 130 164 601.0 0 164 131 157 601.0 0 157 132 185 601.0 0 185 133 114 601.0 0 114 134 143 601.0 0 143 135 114 601.0 0 114 136 118 601.0 0 118 137 134 601.0 0 134 138 205 601.0 0 205 139 161 601.0 0 161 140 155 601.0 0 155 141 117 601.0 0 117 142 200 601.0 0 200 143 134 601.0 0 134 144 175 601.0 0 175 145 142 601.0 0 142 146 123 601.0 0 123 147 122 601.0 0 122 148 218 601.0 0 218 149 86 601.0 0 86 150 99 601.0 0 99 151 109 601.0 0 109 152 111 601.0 0 111 153 103 601.0 0 103 154 112 601.0 0 112 155 104 601.0 0 104 156 108 601.0 0 108 157 100 601.0 0 100 158 138 601.0 0 138 159 105 601.0 0 105 160 93 601.0 0 93 161 121 601.0 0 121 162 79 601.0 0 79 163 72 601.0 0 72 164 84 601.0 0 84 165 94 601.0 0 94 166 93 601.0 0 93 167 74 601.0 0 74 168 88 601.0 0 88 169 82 601.0 0 82 170 70 601.0 0 70 171 99 601.0 0 99 172 76 601.0 0 76 173 82 601.0 0 82 174 89 601.0 0 89 175 72 601.0 0 72 176 86 601.0 0 86 177 92 601.0 0 92 178 81 601.0 0 81 179 147 601.0 0 147 180 126 601.0 0 126 181 95 601.0 0 95 182 96 601.0 0 96 183 84 601.0 0 84 184 100 601.0 0 100 185 89 601.0 0 89 186 92 601.0 0 92 187 100 601.0 0 100 188 95 601.0 0 95 189 104 601.0 0 104 190 101 601.0 0 101 191 101 601.0 0 101 192 187 601.0 0 187 193 206 601.0 0 206 194 116 601.0 0 116 195 106 601.0 0 106 196 87 601.0 0 87 197 250 601.0 0 250 198 391 601.0 0 391 199 789 601.0 0 789 200 106 601.0 0 106 201 86 601.0 0 86 202 87 601.0 0 87 203 86 601.0 0 86 204 229 601.0 0 229 205 112 601.0 0 112 206 103 601.0 0 103 207 69 601.0 0 69 208 63 601.0 0 63 209 76 601.0 0 76 210 92 601.0 0 92 211 69 601.0 0 69 212 93 601.0 0 93 213 80 601.0 0 80 214 86 601.0 0 86 215 93 601.0 0 93 216 88 601.0 0 88 217 104 601.0 0 104 218 82 601.0 0 82 219 90 601.0 0 90 220 89 601.0 0 89 221 107 601.0 0 107 222 165 601.0 0 165 223 298 601.0 0 298 224 139 601.0 0 139 225 518 601.0 0 518 226 110 601.0 0 110 227 79 601.0 0 79 228 82 601.0 0 82 229 90 601.0 0 90 230 87 601.0 0 87 231 105 601.0 0 105 232 109 601.0 0 109 233 109 601.0 0 109 234 99 601.0 0 99 235 80 601.0 0 80 236 99 601.0 0 99 237 91 601.0 0 91 238 125 601.0 0 125 239 106 601.0 0 106 240 138 601.0 0 138 241 144 601.0 0 144 242 126 601.0 0 126 243 92 601.0 0 92 244 97 601.0 0 97 245 156 601.0 0 156 246 143 601.0 0 143 247 108 601.0 0 108 248 83 601.0 0 83 249 91 601.0 0 91 250 66 601.0 0 66 251 108 601.0 0 108 252 94 601.0 0 94 253 98 601.0 0 98 254 105 601.0 0 105 255 138 601.0 0 138 256 151 601.0 0 151 257 125 601.0 0 125 258 108 601.0 0 108 259 134 601.0 0 134 260 130 601.0 0 130 261 137 601.0 0 137 262 126 601.0 0 126 263 126 601.0 0 126 264 118 601.0 0 118 265 375 601.0 0 375 266 464 601.0 0 464 267 374 601.0 0 374 268 144 601.0 0 144 269 69 601.0 0 69 270 79 601.0 0 79 271 94 601.0 0 94 272 135 601.0 0 135 273 175 601.0 0 175 274 174 601.0 0 174 275 119 601.0 0 119 276 92 601.0 0 92 277 137 601.0 0 137 278 126 601.0 0 126 279 158 601.0 0 158 280 205 601.0 0 205 281 222 601.0 0 222 282 235 601.0 0 235 283 187 601.0 0 187 284 275 601.0 0 275 285 315 601.0 0 315 286 384 601.0 0 384 287 250 601.0 0 250 288 411 601.0 0 411 289 350 601.0 0 350 290 389 601.0 0 389 291 695 601.0 0 695 292 656 601.0 0 656 293 591 601.0 0 591 294 581 601.0 0 581 295 555 601.0 0 555 296 654 601.0 0 654 297 2118 601.0 0 2118 298 10994 601.0 0 10994 299 12205 601.0 0 12205 300 11985 601.0 0 11985 301 7195 601.0 0 7195 Finished in 2.12 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 42,735 Reads with adapters: 11,477 (26.9%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 11,463 (26.8%) Total basepairs processed: 10,246,693 bp Total written (filtered): 3,116,915 bp (30.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11477 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 181 0.0 3 0 180 1 20 11214 0.0 4 11008 199 7 21 32 0.0 4 6 26 22 2 0.0 4 2 23 9 0.0 4 1 0 0 8 28 1 0.0 4 1 90 1 0.0 4 0 0 0 0 1 117 1 0.0 4 1 127 17 0.0 4 0 0 0 0 17 138 1 0.0 4 1 152 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 1 184 1 0.0 4 1 185 1 0.0 4 1 186 4 0.0 4 1 2 1 187 1 0.0 4 0 0 0 1 188 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 11,463 Reads with adapters: 2,684 (23.4%) Reads written (passing filters): 11,463 (100.0%) Total basepairs processed: 3,116,915 bp Total written (filtered): 3,091,437 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2684 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 1.9% G: 96.6% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 179.1 0 2 7 1 0.7 0 1 9 1977 0.0 0 52 1925 10 536 0.0 1 18 518 11 164 0.0 1 3 161 12 1 0.0 1 0 1 138 1 0.0 1 1 150 1 0.0 1 0 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.