This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.05 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 139,530 (5.7%) Reads that were too short: 36,304 (1.5%) Reads written (passing filters): 103,226 (4.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 26,987,466 bp (3.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 139530 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1638 601.0 0 1638 7 42365 601.0 0 42365 8 29094 601.0 0 29094 9 91 601.0 0 91 10 80 601.0 0 80 11 80 601.0 0 80 12 105 601.0 0 105 13 68 601.0 0 68 14 85 601.0 0 85 15 63 601.0 0 63 16 70 601.0 0 70 17 79 601.0 0 79 18 73 601.0 0 73 19 76 601.0 0 76 20 64 601.0 0 64 21 81 601.0 0 81 22 74 601.0 0 74 23 75 601.0 0 75 24 100 601.0 0 100 25 76 601.0 0 76 26 79 601.0 0 79 27 68 601.0 0 68 28 76 601.0 0 76 29 86 601.0 0 86 30 87 601.0 0 87 31 76 601.0 0 76 32 75 601.0 0 75 33 69 601.0 0 69 34 72 601.0 0 72 35 99 601.0 0 99 36 102 601.0 0 102 37 103 601.0 0 103 38 82 601.0 0 82 39 113 601.0 0 113 40 116 601.0 0 116 41 125 601.0 0 125 42 102 601.0 0 102 43 100 601.0 0 100 44 184 601.0 0 184 45 67 601.0 0 67 46 96 601.0 0 96 47 96 601.0 0 96 48 72 601.0 0 72 49 112 601.0 0 112 50 78 601.0 0 78 51 109 601.0 0 109 52 100 601.0 0 100 53 111 601.0 0 111 54 117 601.0 0 117 55 114 601.0 0 114 56 110 601.0 0 110 57 95 601.0 0 95 58 112 601.0 0 112 59 114 601.0 0 114 60 101 601.0 0 101 61 65 601.0 0 65 62 114 601.0 0 114 63 139 601.0 0 139 64 1460 601.0 0 1460 65 792 601.0 0 792 66 986 601.0 0 986 67 163 601.0 0 163 68 126 601.0 0 126 69 549 601.0 0 549 70 247 601.0 0 247 71 86 601.0 0 86 72 58 601.0 0 58 73 66 601.0 0 66 74 92 601.0 0 92 75 107 601.0 0 107 76 86 601.0 0 86 77 64 601.0 0 64 78 49 601.0 0 49 79 109 601.0 0 109 80 94 601.0 0 94 81 96 601.0 0 96 82 63 601.0 0 63 83 80 601.0 0 80 84 70 601.0 0 70 85 77 601.0 0 77 86 71 601.0 0 71 87 69 601.0 0 69 88 73 601.0 0 73 89 100 601.0 0 100 90 116 601.0 0 116 91 121 601.0 0 121 92 330 601.0 0 330 93 285 601.0 0 285 94 250 601.0 0 250 95 86 601.0 0 86 96 72 601.0 0 72 97 59 601.0 0 59 98 50 601.0 0 50 99 157 601.0 0 157 100 112 601.0 0 112 101 157 601.0 0 157 102 113 601.0 0 113 103 88 601.0 0 88 104 89 601.0 0 89 105 71 601.0 0 71 106 69 601.0 0 69 107 64 601.0 0 64 108 87 601.0 0 87 109 64 601.0 0 64 110 95 601.0 0 95 111 101 601.0 0 101 112 160 601.0 0 160 113 94 601.0 0 94 114 89 601.0 0 89 115 82 601.0 0 82 116 77 601.0 0 77 117 95 601.0 0 95 118 96 601.0 0 96 119 80 601.0 0 80 120 86 601.0 0 86 121 94 601.0 0 94 122 95 601.0 0 95 123 116 601.0 0 116 124 90 601.0 0 90 125 109 601.0 0 109 126 98 601.0 0 98 127 94 601.0 0 94 128 89 601.0 0 89 129 82 601.0 0 82 130 274 601.0 0 274 131 358 601.0 0 358 132 183 601.0 0 183 133 162 601.0 0 162 134 109 601.0 0 109 135 199 601.0 0 199 136 268 601.0 0 268 137 261 601.0 0 261 138 111 601.0 0 111 139 100 601.0 0 100 140 86 601.0 0 86 141 90 601.0 0 90 142 99 601.0 0 99 143 92 601.0 0 92 144 94 601.0 0 94 145 104 601.0 0 104 146 76 601.0 0 76 147 161 601.0 0 161 148 222 601.0 0 222 149 260 601.0 0 260 150 143 601.0 0 143 151 99 601.0 0 99 152 136 601.0 0 136 153 112 601.0 0 112 154 117 601.0 0 117 155 220 601.0 0 220 156 441 601.0 0 441 157 444 601.0 0 444 158 268 601.0 0 268 159 132 601.0 0 132 160 223 601.0 0 223 161 171 601.0 0 171 162 149 601.0 0 149 163 126 601.0 0 126 164 105 601.0 0 105 165 102 601.0 0 102 166 193 601.0 0 193 167 422 601.0 0 422 168 298 601.0 0 298 169 595 601.0 0 595 170 95 601.0 0 95 171 236 601.0 0 236 172 335 601.0 0 335 173 171 601.0 0 171 174 103 601.0 0 103 175 303 601.0 0 303 176 465 601.0 0 465 177 751 601.0 0 751 178 455 601.0 0 455 179 105 601.0 0 105 180 110 601.0 0 110 181 155 601.0 0 155 182 117 601.0 0 117 183 132 601.0 0 132 184 104 601.0 0 104 185 152 601.0 0 152 186 213 601.0 0 213 187 219 601.0 0 219 188 462 601.0 0 462 189 243 601.0 0 243 190 570 601.0 0 570 191 104 601.0 0 104 192 119 601.0 0 119 193 100 601.0 0 100 194 83 601.0 0 83 195 103 601.0 0 103 196 177 601.0 0 177 197 191 601.0 0 191 198 180 601.0 0 180 199 124 601.0 0 124 200 155 601.0 0 155 201 221 601.0 0 221 202 183 601.0 0 183 203 167 601.0 0 167 204 157 601.0 0 157 205 121 601.0 0 121 206 125 601.0 0 125 207 143 601.0 0 143 208 135 601.0 0 135 209 120 601.0 0 120 210 182 601.0 0 182 211 169 601.0 0 169 212 176 601.0 0 176 213 155 601.0 0 155 214 142 601.0 0 142 215 213 601.0 0 213 216 501 601.0 0 501 217 355 601.0 0 355 218 328 601.0 0 328 219 219 601.0 0 219 220 229 601.0 0 229 221 1111 601.0 0 1111 222 1363 601.0 0 1363 223 1034 601.0 0 1034 224 1126 601.0 0 1126 225 244 601.0 0 244 226 251 601.0 0 251 227 335 601.0 0 335 228 341 601.0 0 341 229 202 601.0 0 202 230 114 601.0 0 114 231 111 601.0 0 111 232 173 601.0 0 173 233 164 601.0 0 164 234 218 601.0 0 218 235 141 601.0 0 141 236 133 601.0 0 133 237 214 601.0 0 214 238 195 601.0 0 195 239 335 601.0 0 335 240 205 601.0 0 205 241 1026 601.0 0 1026 242 972 601.0 0 972 243 1123 601.0 0 1123 244 688 601.0 0 688 245 361 601.0 0 361 246 386 601.0 0 386 247 230 601.0 0 230 248 246 601.0 0 246 249 161 601.0 0 161 250 218 601.0 0 218 251 191 601.0 0 191 252 314 601.0 0 314 253 371 601.0 0 371 254 510 601.0 0 510 255 325 601.0 0 325 256 410 601.0 0 410 257 237 601.0 0 237 258 153 601.0 0 153 259 138 601.0 0 138 260 143 601.0 0 143 261 166 601.0 0 166 262 163 601.0 0 163 263 233 601.0 0 233 264 438 601.0 0 438 265 355 601.0 0 355 266 326 601.0 0 326 267 166 601.0 0 166 268 488 601.0 0 488 269 633 601.0 0 633 270 1512 601.0 0 1512 271 926 601.0 0 926 272 568 601.0 0 568 273 418 601.0 0 418 274 272 601.0 0 272 275 263 601.0 0 263 276 269 601.0 0 269 277 218 601.0 0 218 278 198 601.0 0 198 279 206 601.0 0 206 280 153 601.0 0 153 281 190 601.0 0 190 282 344 601.0 0 344 283 405 601.0 0 405 284 408 601.0 0 408 285 287 601.0 0 287 286 202 601.0 0 202 287 324 601.0 0 324 288 164 601.0 0 164 289 225 601.0 0 225 290 434 601.0 0 434 291 931 601.0 0 931 292 620 601.0 0 620 293 361 601.0 0 361 294 243 601.0 0 243 295 210 601.0 0 210 296 210 601.0 0 210 297 230 601.0 0 230 298 283 601.0 0 283 299 710 601.0 0 710 300 814 601.0 0 814 301 705 601.0 0 705 Finished in 4.63 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 103,226 Reads with adapters: 43,171 (41.8%) Reads that were too short: 143 (0.1%) Reads written (passing filters): 43,028 (41.7%) Total basepairs processed: 26,987,466 bp Total written (filtered): 11,896,307 bp (44.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43171 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 18 0.0 3 0 0 10 8 16 314 0.0 3 0 230 62 22 17 42002 0.0 3 39496 2023 370 113 18 132 0.0 3 6 92 21 13 19 32 0.0 3 0 1 21 10 20 391 0.0 3 0 0 1 390 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 6 0.0 3 0 0 0 6 24 1 0.0 3 0 0 0 1 27 2 0.0 3 1 0 0 1 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 31 5 0.0 3 0 0 0 5 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 1 35 1 0.0 3 0 0 0 1 36 10 0.0 3 0 0 0 10 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 4 0.0 3 0 0 0 4 41 11 0.0 3 0 0 0 11 44 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 11 0.0 3 0 0 0 11 58 1 0.0 3 0 0 0 1 59 6 0.0 3 0 0 0 6 60 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 4 0.0 3 0 0 0 4 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 1 1 69 1 0.0 3 0 0 0 1 71 4 0.0 3 0 0 0 4 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 1 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 1 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 120 3 0.0 3 0 0 0 3 121 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 1 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 3 0.0 3 0 0 0 3 150 4 0.0 3 1 0 0 3 151 12 0.0 3 0 0 0 12 152 2 0.0 3 0 0 0 2 153 1 0.0 3 1 154 1 0.0 3 0 0 1 156 1 0.0 3 0 0 1 157 4 0.0 3 0 0 0 4 158 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 8 0.0 3 0 0 1 7 161 13 0.0 3 0 0 0 13 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 171 2 0.0 3 0 1 0 1 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 192 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 1 223 1 0.0 3 0 0 0 1 224 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 3 0.0 3 0 0 0 3 285 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.52 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 43,028 Reads with adapters: 92 (0.2%) Reads written (passing filters): 43,028 (100.0%) Total basepairs processed: 11,896,307 bp Total written (filtered): 11,896,025 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 92 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.1% C: 7.6% G: 72.8% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 87 672.3 0 87 4 4 168.1 0 4 5 1 42.0 0 1