This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.76 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 102,866 (4.2%) Reads that were too short: 12,106 (0.5%) Reads written (passing filters): 90,760 (3.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 25,206,278 bp (3.4%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 102866 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 917 601.0 0 917 7 32033 601.0 0 32033 8 43579 601.0 0 43579 9 78 601.0 0 78 10 41 601.0 0 41 11 49 601.0 0 49 12 44 601.0 0 44 13 48 601.0 0 48 14 60 601.0 0 60 15 42 601.0 0 42 16 53 601.0 0 53 17 51 601.0 0 51 18 46 601.0 0 46 19 54 601.0 0 54 20 39 601.0 0 39 21 35 601.0 0 35 22 56 601.0 0 56 23 45 601.0 0 45 24 51 601.0 0 51 25 64 601.0 0 64 26 45 601.0 0 45 27 46 601.0 0 46 28 63 601.0 0 63 29 66 601.0 0 66 30 54 601.0 0 54 31 66 601.0 0 66 32 107 601.0 0 107 33 41 601.0 0 41 34 36 601.0 0 36 35 86 601.0 0 86 36 53 601.0 0 53 37 68 601.0 0 68 38 40 601.0 0 40 39 66 601.0 0 66 40 52 601.0 0 52 41 60 601.0 0 60 42 62 601.0 0 62 43 91 601.0 0 91 44 185 601.0 0 185 45 39 601.0 0 39 46 56 601.0 0 56 47 78 601.0 0 78 48 49 601.0 0 49 49 64 601.0 0 64 50 58 601.0 0 58 51 42 601.0 0 42 52 53 601.0 0 53 53 51 601.0 0 51 54 64 601.0 0 64 55 48 601.0 0 48 56 32 601.0 0 32 57 50 601.0 0 50 58 40 601.0 0 40 59 47 601.0 0 47 60 42 601.0 0 42 61 41 601.0 0 41 62 83 601.0 0 83 63 88 601.0 0 88 64 594 601.0 0 594 65 211 601.0 0 211 66 466 601.0 0 466 67 157 601.0 0 157 68 63 601.0 0 63 69 594 601.0 0 594 70 67 601.0 0 67 71 40 601.0 0 40 72 39 601.0 0 39 73 49 601.0 0 49 74 40 601.0 0 40 75 46 601.0 0 46 76 35 601.0 0 35 77 45 601.0 0 45 78 26 601.0 0 26 79 44 601.0 0 44 80 58 601.0 0 58 81 59 601.0 0 59 82 35 601.0 0 35 83 41 601.0 0 41 84 53 601.0 0 53 85 46 601.0 0 46 86 53 601.0 0 53 87 39 601.0 0 39 88 44 601.0 0 44 89 38 601.0 0 38 90 72 601.0 0 72 91 63 601.0 0 63 92 207 601.0 0 207 93 157 601.0 0 157 94 179 601.0 0 179 95 52 601.0 0 52 96 37 601.0 0 37 97 42 601.0 0 42 98 38 601.0 0 38 99 42 601.0 0 42 100 31 601.0 0 31 101 32 601.0 0 32 102 47 601.0 0 47 103 47 601.0 0 47 104 49 601.0 0 49 105 40 601.0 0 40 106 35 601.0 0 35 107 38 601.0 0 38 108 47 601.0 0 47 109 59 601.0 0 59 110 39 601.0 0 39 111 71 601.0 0 71 112 49 601.0 0 49 113 34 601.0 0 34 114 45 601.0 0 45 115 49 601.0 0 49 116 39 601.0 0 39 117 69 601.0 0 69 118 57 601.0 0 57 119 43 601.0 0 43 120 66 601.0 0 66 121 60 601.0 0 60 122 53 601.0 0 53 123 48 601.0 0 48 124 53 601.0 0 53 125 54 601.0 0 54 126 57 601.0 0 57 127 45 601.0 0 45 128 57 601.0 0 57 129 48 601.0 0 48 130 81 601.0 0 81 131 73 601.0 0 73 132 66 601.0 0 66 133 64 601.0 0 64 134 60 601.0 0 60 135 55 601.0 0 55 136 78 601.0 0 78 137 100 601.0 0 100 138 63 601.0 0 63 139 46 601.0 0 46 140 50 601.0 0 50 141 55 601.0 0 55 142 55 601.0 0 55 143 34 601.0 0 34 144 61 601.0 0 61 145 39 601.0 0 39 146 41 601.0 0 41 147 59 601.0 0 59 148 100 601.0 0 100 149 90 601.0 0 90 150 91 601.0 0 91 151 60 601.0 0 60 152 59 601.0 0 59 153 95 601.0 0 95 154 52 601.0 0 52 155 80 601.0 0 80 156 155 601.0 0 155 157 123 601.0 0 123 158 87 601.0 0 87 159 69 601.0 0 69 160 103 601.0 0 103 161 69 601.0 0 69 162 57 601.0 0 57 163 64 601.0 0 64 164 59 601.0 0 59 165 45 601.0 0 45 166 77 601.0 0 77 167 188 601.0 0 188 168 98 601.0 0 98 169 168 601.0 0 168 170 52 601.0 0 52 171 101 601.0 0 101 172 128 601.0 0 128 173 82 601.0 0 82 174 54 601.0 0 54 175 141 601.0 0 141 176 142 601.0 0 142 177 146 601.0 0 146 178 78 601.0 0 78 179 50 601.0 0 50 180 61 601.0 0 61 181 63 601.0 0 63 182 59 601.0 0 59 183 65 601.0 0 65 184 45 601.0 0 45 185 63 601.0 0 63 186 78 601.0 0 78 187 54 601.0 0 54 188 141 601.0 0 141 189 93 601.0 0 93 190 253 601.0 0 253 191 89 601.0 0 89 192 55 601.0 0 55 193 67 601.0 0 67 194 48 601.0 0 48 195 70 601.0 0 70 196 66 601.0 0 66 197 70 601.0 0 70 198 65 601.0 0 65 199 63 601.0 0 63 200 72 601.0 0 72 201 48 601.0 0 48 202 73 601.0 0 73 203 73 601.0 0 73 204 90 601.0 0 90 205 102 601.0 0 102 206 88 601.0 0 88 207 95 601.0 0 95 208 70 601.0 0 70 209 61 601.0 0 61 210 76 601.0 0 76 211 77 601.0 0 77 212 104 601.0 0 104 213 107 601.0 0 107 214 80 601.0 0 80 215 84 601.0 0 84 216 100 601.0 0 100 217 93 601.0 0 93 218 73 601.0 0 73 219 80 601.0 0 80 220 98 601.0 0 98 221 213 601.0 0 213 222 235 601.0 0 235 223 175 601.0 0 175 224 162 601.0 0 162 225 117 601.0 0 117 226 94 601.0 0 94 227 129 601.0 0 129 228 124 601.0 0 124 229 84 601.0 0 84 230 53 601.0 0 53 231 67 601.0 0 67 232 69 601.0 0 69 233 74 601.0 0 74 234 99 601.0 0 99 235 89 601.0 0 89 236 89 601.0 0 89 237 75 601.0 0 75 238 132 601.0 0 132 239 106 601.0 0 106 240 97 601.0 0 97 241 297 601.0 0 297 242 209 601.0 0 209 243 255 601.0 0 255 244 212 601.0 0 212 245 142 601.0 0 142 246 93 601.0 0 93 247 106 601.0 0 106 248 119 601.0 0 119 249 77 601.0 0 77 250 99 601.0 0 99 251 114 601.0 0 114 252 109 601.0 0 109 253 146 601.0 0 146 254 171 601.0 0 171 255 106 601.0 0 106 256 110 601.0 0 110 257 81 601.0 0 81 258 69 601.0 0 69 259 77 601.0 0 77 260 73 601.0 0 73 261 59 601.0 0 59 262 67 601.0 0 67 263 94 601.0 0 94 264 102 601.0 0 102 265 97 601.0 0 97 266 111 601.0 0 111 267 82 601.0 0 82 268 212 601.0 0 212 269 223 601.0 0 223 270 260 601.0 0 260 271 218 601.0 0 218 272 144 601.0 0 144 273 162 601.0 0 162 274 107 601.0 0 107 275 113 601.0 0 113 276 111 601.0 0 111 277 152 601.0 0 152 278 130 601.0 0 130 279 160 601.0 0 160 280 74 601.0 0 74 281 83 601.0 0 83 282 102 601.0 0 102 283 130 601.0 0 130 284 124 601.0 0 124 285 86 601.0 0 86 286 91 601.0 0 91 287 104 601.0 0 104 288 91 601.0 0 91 289 106 601.0 0 106 290 120 601.0 0 120 291 204 601.0 0 204 292 202 601.0 0 202 293 131 601.0 0 131 294 86 601.0 0 86 295 85 601.0 0 85 296 104 601.0 0 104 297 147 601.0 0 147 298 172 601.0 0 172 299 181 601.0 0 181 300 263 601.0 0 263 301 244 601.0 0 244 Finished in 4.65 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 90,760 Reads with adapters: 44,639 (49.2%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 44,630 (49.2%) Total basepairs processed: 25,206,278 bp Total written (filtered): 12,182,962 bp (48.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 44639 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 17 0.0 3 0 0 17 19 363 0.0 3 0 359 3 1 20 43999 0.0 4 43401 579 17 1 1 21 140 0.0 4 23 113 4 22 14 0.0 4 2 3 7 0 2 23 60 0.0 4 27 0 1 30 2 24 1 0.0 4 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 1 0 0 1 34 1 0.0 4 0 0 0 0 1 35 12 0.0 4 0 0 0 0 12 66 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 127 13 0.0 4 0 0 0 0 13 132 1 0.0 4 1 136 1 0.0 4 1 143 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 154 1 0.0 4 0 0 0 1 155 1 0.0 4 1 156 2 0.0 4 0 0 2 176 1 0.0 4 0 0 1 224 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.57 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 44,630 Reads with adapters: 3,037 (6.8%) Reads written (passing filters): 44,630 (100.0%) Total basepairs processed: 12,182,962 bp Total written (filtered): 12,138,773 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3037 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.8% C: 4.3% G: 87.7% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 697.3 0 25 4 9 174.3 0 9 5 2 43.6 0 2 6 1 10.9 0 1 9 13 0.2 0 1 12 10 102 0.0 1 3 99 11 2705 0.0 1 17 2688 12 2 0.0 1 0 2 14 2 0.0 1 2 19 74 0.0 1 54 20 20 7 0.0 1 7 21 3 0.0 1 2 1 23 24 0.0 1 20 4 24 5 0.0 1 5 25 1 0.0 1 1 90 1 0.0 1 0 1 107 1 0.0 1 1 108 1 0.0 1 1 115 11 0.0 1 9 2 116 2 0.0 1 2 123 3 0.0 1 3 151 6 0.0 1 0 6 209 35 0.0 1 13 22 210 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.