This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.58 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 468,968 (19.1%) Reads that were too short: 430,367 (17.5%) Reads written (passing filters): 38,601 (1.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,418,072 bp (1.1%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 468968 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 161 601.0 0 161 7 8916 601.0 0 8916 8 180 601.0 0 180 9 5455 601.0 0 5455 10 82 601.0 0 82 11 59 601.0 0 59 12 67 601.0 0 67 13 63 601.0 0 63 14 83 601.0 0 83 15 73 601.0 0 73 16 73 601.0 0 73 17 62 601.0 0 62 18 66 601.0 0 66 19 50 601.0 0 50 20 57 601.0 0 57 21 45 601.0 0 45 22 46 601.0 0 46 23 42 601.0 0 42 24 57 601.0 0 57 25 70 601.0 0 70 26 50 601.0 0 50 27 70 601.0 0 70 28 82 601.0 0 82 29 52 601.0 0 52 30 57 601.0 0 57 31 103 601.0 0 103 32 61 601.0 0 61 33 65 601.0 0 65 34 51 601.0 0 51 35 42 601.0 0 42 36 77 601.0 0 77 37 72 601.0 0 72 38 144 601.0 0 144 39 54 601.0 0 54 40 57 601.0 0 57 41 43 601.0 0 43 42 46 601.0 0 46 43 58 601.0 0 58 44 55 601.0 0 55 45 77 601.0 0 77 46 55 601.0 0 55 47 52 601.0 0 52 48 46 601.0 0 46 49 51 601.0 0 51 50 45 601.0 0 45 51 44 601.0 0 44 52 53 601.0 0 53 53 54 601.0 0 54 54 65 601.0 0 65 55 40 601.0 0 40 56 54 601.0 0 54 57 57 601.0 0 57 58 46 601.0 0 46 59 63 601.0 0 63 60 84 601.0 0 84 61 33 601.0 0 33 62 36 601.0 0 36 63 51 601.0 0 51 64 47 601.0 0 47 65 39 601.0 0 39 66 56 601.0 0 56 67 63 601.0 0 63 68 57 601.0 0 57 69 168 601.0 0 168 70 88 601.0 0 88 71 70 601.0 0 70 72 64 601.0 0 64 73 106 601.0 0 106 74 59 601.0 0 59 75 59 601.0 0 59 76 154 601.0 0 154 77 62 601.0 0 62 78 46 601.0 0 46 79 64 601.0 0 64 80 62 601.0 0 62 81 52 601.0 0 52 82 72 601.0 0 72 83 98 601.0 0 98 84 64 601.0 0 64 85 67 601.0 0 67 86 81 601.0 0 81 87 59 601.0 0 59 88 58 601.0 0 58 89 63 601.0 0 63 90 50 601.0 0 50 91 52 601.0 0 52 92 54 601.0 0 54 93 56 601.0 0 56 94 55 601.0 0 55 95 77 601.0 0 77 96 69 601.0 0 69 97 89 601.0 0 89 98 81 601.0 0 81 99 84 601.0 0 84 100 87 601.0 0 87 101 66 601.0 0 66 102 58 601.0 0 58 103 75 601.0 0 75 104 67 601.0 0 67 105 73 601.0 0 73 106 85 601.0 0 85 107 101 601.0 0 101 108 97 601.0 0 97 109 68 601.0 0 68 110 74 601.0 0 74 111 106 601.0 0 106 112 79 601.0 0 79 113 55 601.0 0 55 114 88 601.0 0 88 115 97 601.0 0 97 116 77 601.0 0 77 117 73 601.0 0 73 118 969 601.0 0 969 119 108 601.0 0 108 120 268 601.0 0 268 121 105 601.0 0 105 122 84 601.0 0 84 123 102 601.0 0 102 124 99 601.0 0 99 125 116 601.0 0 116 126 95 601.0 0 95 127 71 601.0 0 71 128 68 601.0 0 68 129 75 601.0 0 75 130 74 601.0 0 74 131 97 601.0 0 97 132 102 601.0 0 102 133 106 601.0 0 106 134 93 601.0 0 93 135 84 601.0 0 84 136 80 601.0 0 80 137 95 601.0 0 95 138 93 601.0 0 93 139 107 601.0 0 107 140 117 601.0 0 117 141 137 601.0 0 137 142 112 601.0 0 112 143 78 601.0 0 78 144 54 601.0 0 54 145 84 601.0 0 84 146 93 601.0 0 93 147 101 601.0 0 101 148 80 601.0 0 80 149 75 601.0 0 75 150 60 601.0 0 60 151 51 601.0 0 51 152 51 601.0 0 51 153 79 601.0 0 79 154 126 601.0 0 126 155 108 601.0 0 108 156 117 601.0 0 117 157 189 601.0 0 189 158 411 601.0 0 411 159 5366 601.0 0 5366 160 1031 601.0 0 1031 161 1733 601.0 0 1733 162 68 601.0 0 68 163 66 601.0 0 66 164 71 601.0 0 71 165 79 601.0 0 79 166 70 601.0 0 70 167 96 601.0 0 96 168 118 601.0 0 118 169 75 601.0 0 75 170 86 601.0 0 86 171 67 601.0 0 67 172 87 601.0 0 87 173 114 601.0 0 114 174 100 601.0 0 100 175 72 601.0 0 72 176 81 601.0 0 81 177 82 601.0 0 82 178 57 601.0 0 57 179 64 601.0 0 64 180 78 601.0 0 78 181 86 601.0 0 86 182 62 601.0 0 62 183 51 601.0 0 51 184 72 601.0 0 72 185 78 601.0 0 78 186 76 601.0 0 76 187 74 601.0 0 74 188 94 601.0 0 94 189 75 601.0 0 75 190 65 601.0 0 65 191 61 601.0 0 61 192 79 601.0 0 79 193 87 601.0 0 87 194 83 601.0 0 83 195 103 601.0 0 103 196 97 601.0 0 97 197 154 601.0 0 154 198 124 601.0 0 124 199 118 601.0 0 118 200 94 601.0 0 94 201 70 601.0 0 70 202 89 601.0 0 89 203 104 601.0 0 104 204 87 601.0 0 87 205 96 601.0 0 96 206 188 601.0 0 188 207 252 601.0 0 252 208 151 601.0 0 151 209 125 601.0 0 125 210 142 601.0 0 142 211 174 601.0 0 174 212 282 601.0 0 282 213 165 601.0 0 165 214 148 601.0 0 148 215 108 601.0 0 108 216 89 601.0 0 89 217 125 601.0 0 125 218 510 601.0 0 510 219 175 601.0 0 175 220 129 601.0 0 129 221 161 601.0 0 161 222 132 601.0 0 132 223 111 601.0 0 111 224 134 601.0 0 134 225 258 601.0 0 258 226 150 601.0 0 150 227 209 601.0 0 209 228 797 601.0 0 797 229 171 601.0 0 171 230 111 601.0 0 111 231 120 601.0 0 120 232 384 601.0 0 384 233 349 601.0 0 349 234 275 601.0 0 275 235 173 601.0 0 173 236 211 601.0 0 211 237 446 601.0 0 446 238 1062 601.0 0 1062 239 276 601.0 0 276 240 404 601.0 0 404 241 253 601.0 0 253 242 260 601.0 0 260 243 600 601.0 0 600 244 311 601.0 0 311 245 291 601.0 0 291 246 221 601.0 0 221 247 290 601.0 0 290 248 530 601.0 0 530 249 703 601.0 0 703 250 1177 601.0 0 1177 251 1828 601.0 0 1828 252 2338 601.0 0 2338 253 3975 601.0 0 3975 254 10877 601.0 0 10877 255 88118 601.0 0 88118 256 136432 601.0 0 136432 257 167556 601.0 0 167556 258 2011 601.0 0 2011 259 151 601.0 0 151 260 102 601.0 0 102 261 112 601.0 0 112 262 189 601.0 0 189 263 154 601.0 0 154 264 100 601.0 0 100 265 70 601.0 0 70 266 121 601.0 0 121 267 95 601.0 0 95 268 82 601.0 0 82 269 51 601.0 0 51 270 59 601.0 0 59 271 50 601.0 0 50 272 69 601.0 0 69 273 83 601.0 0 83 274 89 601.0 0 89 275 66 601.0 0 66 276 61 601.0 0 61 277 79 601.0 0 79 278 80 601.0 0 80 279 72 601.0 0 72 280 49 601.0 0 49 281 63 601.0 0 63 282 70 601.0 0 70 283 70 601.0 0 70 284 86 601.0 0 86 285 53 601.0 0 53 286 55 601.0 0 55 287 47 601.0 0 47 288 72 601.0 0 72 289 48 601.0 0 48 290 57 601.0 0 57 291 72 601.0 0 72 292 74 601.0 0 74 293 41 601.0 0 41 294 70 601.0 0 70 295 72 601.0 0 72 296 64 601.0 0 64 297 62 601.0 0 62 298 69 601.0 0 69 299 87 601.0 0 87 300 110 601.0 0 110 301 197 601.0 0 197 Finished in 1.69 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 38,601 Reads with adapters: 8,940 (23.2%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 8,934 (23.1%) Total basepairs processed: 8,418,072 bp Total written (filtered): 2,447,291 bp (29.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8940 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 90 0.0 3 0 87 3 20 8787 0.0 4 8640 142 5 21 39 0.0 4 18 21 23 6 0.0 4 1 0 0 5 26 1 0.0 4 1 100 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 114 1 0.0 4 1 115 1 0.0 4 1 117 1 0.0 4 1 126 1 0.0 4 0 0 0 0 1 127 4 0.0 4 0 0 0 0 4 136 1 0.0 4 0 0 0 1 159 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 8,934 Reads with adapters: 73 (0.8%) Reads written (passing filters): 8,934 (100.0%) Total basepairs processed: 2,447,291 bp Total written (filtered): 2,446,488 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 73 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 32.9% C: 2.7% G: 63.0% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 139.6 0 11 4 1 34.9 0 1 6 1 2.2 0 1 9 2 0.0 0 1 1 11 41 0.0 1 0 41 12 6 0.0 1 0 6 15 1 0.0 1 0 1 19 1 0.0 1 0 1 20 7 0.0 1 7 21 1 0.0 1 0 1 24 1 0.0 1 1