This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.12 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 98,722 (4.0%) Reads that were too short: 45,893 (1.9%) Reads written (passing filters): 52,829 (2.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 7,965,263 bp (1.1%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 98722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 596.3 0 402 7 200 596.3 0 200 8 2744 596.3 0 2744 9 2184 596.3 0 2184 10 28 596.3 0 28 11 22 596.3 0 22 12 20 596.3 0 20 13 21 596.3 0 21 14 24 596.3 0 24 15 19 596.3 0 19 16 29 596.3 0 29 17 34 596.3 0 34 18 35 596.3 0 35 19 26 596.3 0 26 20 26 596.3 0 26 21 42 596.3 0 42 22 39 596.3 0 39 23 46 596.3 0 46 24 30 596.3 0 30 25 24 596.3 0 24 26 72 596.3 0 72 27 69 596.3 0 69 28 28 596.3 0 28 29 51 596.3 0 51 30 26 596.3 0 26 31 24 596.3 0 24 32 31 596.3 0 31 33 42 596.3 0 42 34 32 596.3 0 32 35 26 596.3 0 26 36 23 596.3 0 23 37 33 596.3 0 33 38 24 596.3 0 24 39 26 596.3 0 26 40 22 596.3 0 22 41 31 596.3 0 31 42 23 596.3 0 23 43 30 596.3 0 30 44 25 596.3 0 25 45 25 596.3 0 25 46 24 596.3 0 24 47 25 596.3 0 25 48 20 596.3 0 20 49 20 596.3 0 20 50 22 596.3 0 22 51 43 596.3 0 43 52 34 596.3 0 34 53 21 596.3 0 21 54 42 596.3 0 42 55 22 596.3 0 22 56 15 596.3 0 15 57 37 596.3 0 37 58 18 596.3 0 18 59 31 596.3 0 31 60 14 596.3 0 14 61 32 596.3 0 32 62 18 596.3 0 18 63 17 596.3 0 17 64 47 596.3 0 47 65 40 596.3 0 40 66 66 596.3 0 66 67 31 596.3 0 31 68 33 596.3 0 33 69 55 596.3 0 55 70 67 596.3 0 67 71 86 596.3 0 86 72 20 596.3 0 20 73 22 596.3 0 22 74 40 596.3 0 40 75 36 596.3 0 36 76 51 596.3 0 51 77 60 596.3 0 60 78 27 596.3 0 27 79 81 596.3 0 81 80 146 596.3 0 146 81 226 596.3 0 226 82 56 596.3 0 56 83 35 596.3 0 35 84 50 596.3 0 50 85 39 596.3 0 39 86 34 596.3 0 34 87 29 596.3 0 29 88 29 596.3 0 29 89 48 596.3 0 48 90 29 596.3 0 29 91 31 596.3 0 31 92 28 596.3 0 28 93 23 596.3 0 23 94 34 596.3 0 34 95 43 596.3 0 43 96 35 596.3 0 35 97 34 596.3 0 34 98 35 596.3 0 35 99 59 596.3 0 59 100 72 596.3 0 72 101 98 596.3 0 98 102 39 596.3 0 39 103 25 596.3 0 25 104 35 596.3 0 35 105 32 596.3 0 32 106 31 596.3 0 31 107 47 596.3 0 47 108 31 596.3 0 31 109 28 596.3 0 28 110 46 596.3 0 46 111 62 596.3 0 62 112 104 596.3 0 104 113 143 596.3 0 143 114 94 596.3 0 94 115 49 596.3 0 49 116 44 596.3 0 44 117 308 596.3 0 308 118 278 596.3 0 278 119 181 596.3 0 181 120 55 596.3 0 55 121 48 596.3 0 48 122 60 596.3 0 60 123 52 596.3 0 52 124 39 596.3 0 39 125 45 596.3 0 45 126 47 596.3 0 47 127 73 596.3 0 73 128 181 596.3 0 181 129 153 596.3 0 153 130 320 596.3 0 320 131 329 596.3 0 329 132 363 596.3 0 363 133 294 596.3 0 294 134 85 596.3 0 85 135 115 596.3 0 115 136 160 596.3 0 160 137 287 596.3 0 287 138 236 596.3 0 236 139 89 596.3 0 89 140 75 596.3 0 75 141 51 596.3 0 51 142 37 596.3 0 37 143 50 596.3 0 50 144 41 596.3 0 41 145 64 596.3 0 64 146 111 596.3 0 111 147 106 596.3 0 106 148 42 596.3 0 42 149 44 596.3 0 44 150 46 596.3 0 46 151 41 596.3 0 41 152 44 596.3 0 44 153 43 596.3 0 43 154 80 596.3 0 80 155 47 596.3 0 47 156 76 596.3 0 76 157 133 596.3 0 133 158 195 596.3 0 195 159 54 596.3 0 54 160 69 596.3 0 69 161 79 596.3 0 79 162 93 596.3 0 93 163 95 596.3 0 95 164 86 596.3 0 86 165 103 596.3 0 103 166 116 596.3 0 116 167 114 596.3 0 114 168 173 596.3 0 173 169 157 596.3 0 157 170 124 596.3 0 124 171 162 596.3 0 162 172 116 596.3 0 116 173 146 596.3 0 146 174 163 596.3 0 163 175 221 596.3 0 221 176 818 596.3 0 818 177 3864 596.3 0 3864 178 7367 596.3 0 7367 179 1603 596.3 0 1603 180 2728 596.3 0 2728 181 3281 596.3 0 3281 182 4475 596.3 0 4475 183 3420 596.3 0 3420 184 867 596.3 0 867 185 210 596.3 0 210 186 103 596.3 0 103 187 83 596.3 0 83 188 92 596.3 0 92 189 122 596.3 0 122 190 85 596.3 0 85 191 91 596.3 0 91 192 100 596.3 0 100 193 95 596.3 0 95 194 136 596.3 0 136 195 387 596.3 0 387 196 1478 596.3 0 1478 197 1884 596.3 0 1884 198 1296 596.3 0 1296 199 451 596.3 0 451 200 134 596.3 0 134 201 566 596.3 0 566 202 1353 596.3 0 1353 203 1635 596.3 0 1635 204 686 596.3 0 686 205 48 596.3 0 48 206 40 596.3 0 40 207 36 596.3 0 36 208 51 596.3 0 51 209 63 596.3 0 63 210 47 596.3 0 47 211 38 596.3 0 38 212 32 596.3 0 32 213 51 596.3 0 51 214 51 596.3 0 51 215 121 596.3 0 121 216 172 596.3 0 172 217 179 596.3 0 179 218 115 596.3 0 115 219 75 596.3 0 75 220 47 596.3 0 47 221 91 596.3 0 91 222 345 596.3 0 345 223 734 596.3 0 734 224 726 596.3 0 726 225 370 596.3 0 370 226 75 596.3 0 75 227 54 596.3 0 54 228 52 596.3 0 52 229 50 596.3 0 50 230 47 596.3 0 47 231 70 596.3 0 70 232 53 596.3 0 53 233 100 596.3 0 100 234 196 596.3 0 196 235 302 596.3 0 302 236 459 596.3 0 459 237 161 596.3 0 161 238 121 596.3 0 121 239 545 596.3 0 545 240 1116 596.3 0 1116 241 1225 596.3 0 1225 242 419 596.3 0 419 243 448 596.3 0 448 244 348 596.3 0 348 245 304 596.3 0 304 246 142 596.3 0 142 247 94 596.3 0 94 248 79 596.3 0 79 249 104 596.3 0 104 250 146 596.3 0 146 251 440 596.3 0 440 252 319 596.3 0 319 253 225 596.3 0 225 254 206 596.3 0 206 255 204 596.3 0 204 256 239 596.3 0 239 257 556 596.3 0 556 258 678 596.3 0 678 259 1682 596.3 0 1682 260 5774 596.3 0 5774 261 2457 596.3 0 2457 262 278 596.3 0 278 263 297 596.3 0 297 264 1231 596.3 0 1231 265 3813 596.3 0 3813 266 3080 596.3 0 3080 267 1816 596.3 0 1816 268 36 596.3 0 36 269 69 596.3 0 69 270 105 596.3 0 105 271 74 596.3 0 74 272 88 596.3 0 88 273 92 596.3 0 92 274 142 596.3 0 142 275 263 596.3 0 263 276 207 596.3 0 207 277 266 596.3 0 266 278 325 596.3 0 325 279 223 596.3 0 223 280 51 596.3 0 51 281 35 596.3 0 35 282 41 596.3 0 41 283 55 596.3 0 55 284 48 596.3 0 48 285 76 596.3 0 76 286 67 596.3 0 67 287 83 596.3 0 83 288 75 596.3 0 75 289 72 596.3 0 72 290 135 596.3 0 135 291 1012 596.3 0 1012 292 1843 596.3 0 1843 293 1645 596.3 0 1645 294 425 596.3 0 425 295 102 596.3 0 102 296 129 596.3 0 129 297 233 596.3 0 233 298 158 596.3 0 158 299 175 596.3 0 175 300 243 596.3 0 243 301 189 596.3 0 189 Finished in 1.67 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 52,829 Reads with adapters: 3,851 (7.3%) Reads that were too short: 713 (1.3%) Reads written (passing filters): 3,138 (5.9%) Total basepairs processed: 7,965,263 bp Total written (filtered): 836,695 bp (10.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3851 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 3 16 40 0.0 3 0 27 2 11 17 2490 0.0 3 2310 149 20 11 18 75 0.0 3 34 21 12 8 19 25 0.0 3 5 7 6 7 20 331 0.0 3 289 24 4 14 21 2 0.0 3 0 1 0 1 22 2 0.0 3 1 0 0 1 27 11 0.0 3 0 0 0 11 29 1 0.0 3 0 0 0 1 30 4 0.0 3 0 0 0 4 32 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 31 0.0 3 0 0 0 31 38 7 0.0 3 0 0 0 7 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 16 0.0 3 0 0 0 16 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 50 363 0.0 3 0 0 5 358 51 3 0.0 3 0 0 0 3 53 2 0.0 3 0 0 0 2 54 11 0.0 3 0 0 0 11 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 60 29 0.0 3 0 0 0 29 61 1 0.0 3 0 0 0 1 62 11 0.0 3 0 0 0 11 63 14 0.0 3 0 0 1 13 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 5 0.0 3 0 0 0 5 75 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 79 1 0.0 3 0 0 0 1 80 4 0.0 3 0 0 0 4 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 7 0.0 3 0 0 0 7 91 10 0.0 3 0 0 0 10 92 7 0.0 3 0 0 0 7 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 8 0.0 3 0 0 0 8 100 146 0.0 3 0 0 11 135 101 10 0.0 3 0 0 0 10 102 17 0.0 3 0 0 0 17 103 4 0.0 3 0 0 0 4 104 5 0.0 3 0 0 0 5 105 12 0.0 3 0 0 1 11 106 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 12 0.0 3 0 0 0 12 121 3 0.0 3 0 0 1 2 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 130 19 0.0 3 0 0 1 18 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 136 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 5 0.0 3 0 0 0 5 158 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 1 265 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 3,138 Reads with adapters: 32 (1.0%) Reads written (passing filters): 3,138 (100.0%) Total basepairs processed: 836,695 bp Total written (filtered): 836,596 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 32 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.6% C: 21.9% G: 31.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 30 49.0 0 30 4 1 12.3 0 1 5 1 3.1 0 1