This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.78 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 75,601 (3.1%) Reads that were too short: 33,077 (1.4%) Reads written (passing filters): 42,524 (1.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 6,458,844 bp (0.9%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 75601 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 364 596.3 0 364 7 113 596.3 0 113 8 2398 596.3 0 2398 9 2553 596.3 0 2553 10 14 596.3 0 14 11 19 596.3 0 19 12 18 596.3 0 18 13 20 596.3 0 20 14 5 596.3 0 5 15 14 596.3 0 14 16 20 596.3 0 20 17 18 596.3 0 18 18 15 596.3 0 15 19 14 596.3 0 14 20 16 596.3 0 16 21 31 596.3 0 31 22 22 596.3 0 22 23 22 596.3 0 22 24 17 596.3 0 17 25 7 596.3 0 7 26 21 596.3 0 21 27 23 596.3 0 23 28 29 596.3 0 29 29 44 596.3 0 44 30 26 596.3 0 26 31 15 596.3 0 15 32 10 596.3 0 10 33 28 596.3 0 28 34 23 596.3 0 23 35 18 596.3 0 18 36 26 596.3 0 26 37 36 596.3 0 36 38 25 596.3 0 25 39 13 596.3 0 13 40 25 596.3 0 25 41 31 596.3 0 31 42 13 596.3 0 13 43 9 596.3 0 9 44 26 596.3 0 26 45 19 596.3 0 19 46 16 596.3 0 16 47 27 596.3 0 27 48 16 596.3 0 16 49 18 596.3 0 18 50 27 596.3 0 27 51 19 596.3 0 19 52 17 596.3 0 17 53 22 596.3 0 22 54 21 596.3 0 21 55 23 596.3 0 23 56 18 596.3 0 18 57 18 596.3 0 18 58 14 596.3 0 14 59 19 596.3 0 19 60 16 596.3 0 16 61 21 596.3 0 21 62 17 596.3 0 17 63 18 596.3 0 18 64 23 596.3 0 23 65 32 596.3 0 32 66 38 596.3 0 38 67 24 596.3 0 24 68 21 596.3 0 21 69 31 596.3 0 31 70 82 596.3 0 82 71 81 596.3 0 81 72 15 596.3 0 15 73 20 596.3 0 20 74 24 596.3 0 24 75 20 596.3 0 20 76 21 596.3 0 21 77 20 596.3 0 20 78 27 596.3 0 27 79 55 596.3 0 55 80 105 596.3 0 105 81 159 596.3 0 159 82 21 596.3 0 21 83 13 596.3 0 13 84 11 596.3 0 11 85 10 596.3 0 10 86 14 596.3 0 14 87 15 596.3 0 15 88 15 596.3 0 15 89 17 596.3 0 17 90 16 596.3 0 16 91 17 596.3 0 17 92 13 596.3 0 13 93 20 596.3 0 20 94 14 596.3 0 14 95 16 596.3 0 16 96 15 596.3 0 15 97 20 596.3 0 20 98 23 596.3 0 23 99 37 596.3 0 37 100 31 596.3 0 31 101 41 596.3 0 41 102 19 596.3 0 19 103 28 596.3 0 28 104 18 596.3 0 18 105 14 596.3 0 14 106 21 596.3 0 21 107 13 596.3 0 13 108 18 596.3 0 18 109 14 596.3 0 14 110 18 596.3 0 18 111 30 596.3 0 30 112 38 596.3 0 38 113 92 596.3 0 92 114 56 596.3 0 56 115 19 596.3 0 19 116 26 596.3 0 26 117 102 596.3 0 102 118 105 596.3 0 105 119 55 596.3 0 55 120 22 596.3 0 22 121 28 596.3 0 28 122 18 596.3 0 18 123 21 596.3 0 21 124 22 596.3 0 22 125 28 596.3 0 28 126 31 596.3 0 31 127 41 596.3 0 41 128 92 596.3 0 92 129 131 596.3 0 131 130 221 596.3 0 221 131 81 596.3 0 81 132 109 596.3 0 109 133 88 596.3 0 88 134 58 596.3 0 58 135 87 596.3 0 87 136 124 596.3 0 124 137 275 596.3 0 275 138 170 596.3 0 170 139 51 596.3 0 51 140 47 596.3 0 47 141 21 596.3 0 21 142 16 596.3 0 16 143 33 596.3 0 33 144 36 596.3 0 36 145 37 596.3 0 37 146 66 596.3 0 66 147 45 596.3 0 45 148 38 596.3 0 38 149 26 596.3 0 26 150 24 596.3 0 24 151 30 596.3 0 30 152 28 596.3 0 28 153 28 596.3 0 28 154 34 596.3 0 34 155 35 596.3 0 35 156 43 596.3 0 43 157 91 596.3 0 91 158 117 596.3 0 117 159 26 596.3 0 26 160 49 596.3 0 49 161 38 596.3 0 38 162 71 596.3 0 71 163 107 596.3 0 107 164 59 596.3 0 59 165 102 596.3 0 102 166 109 596.3 0 109 167 115 596.3 0 115 168 153 596.3 0 153 169 114 596.3 0 114 170 102 596.3 0 102 171 168 596.3 0 168 172 87 596.3 0 87 173 132 596.3 0 132 174 122 596.3 0 122 175 143 596.3 0 143 176 635 596.3 0 635 177 3510 596.3 0 3510 178 6761 596.3 0 6761 179 1438 596.3 0 1438 180 2857 596.3 0 2857 181 3192 596.3 0 3192 182 4128 596.3 0 4128 183 3149 596.3 0 3149 184 316 596.3 0 316 185 90 596.3 0 90 186 47 596.3 0 47 187 43 596.3 0 43 188 42 596.3 0 42 189 54 596.3 0 54 190 25 596.3 0 25 191 50 596.3 0 50 192 57 596.3 0 57 193 52 596.3 0 52 194 68 596.3 0 68 195 183 596.3 0 183 196 666 596.3 0 666 197 971 596.3 0 971 198 958 596.3 0 958 199 304 596.3 0 304 200 85 596.3 0 85 201 283 596.3 0 283 202 738 596.3 0 738 203 1151 596.3 0 1151 204 294 596.3 0 294 205 18 596.3 0 18 206 21 596.3 0 21 207 32 596.3 0 32 208 21 596.3 0 21 209 32 596.3 0 32 210 29 596.3 0 29 211 21 596.3 0 21 212 27 596.3 0 27 213 35 596.3 0 35 214 32 596.3 0 32 215 76 596.3 0 76 216 115 596.3 0 115 217 90 596.3 0 90 218 74 596.3 0 74 219 31 596.3 0 31 220 24 596.3 0 24 221 48 596.3 0 48 222 166 596.3 0 166 223 386 596.3 0 386 224 398 596.3 0 398 225 263 596.3 0 263 226 58 596.3 0 58 227 29 596.3 0 29 228 25 596.3 0 25 229 41 596.3 0 41 230 38 596.3 0 38 231 37 596.3 0 37 232 44 596.3 0 44 233 44 596.3 0 44 234 166 596.3 0 166 235 335 596.3 0 335 236 461 596.3 0 461 237 174 596.3 0 174 238 56 596.3 0 56 239 420 596.3 0 420 240 708 596.3 0 708 241 935 596.3 0 935 242 199 596.3 0 199 243 183 596.3 0 183 244 111 596.3 0 111 245 224 596.3 0 224 246 105 596.3 0 105 247 69 596.3 0 69 248 61 596.3 0 61 249 90 596.3 0 90 250 116 596.3 0 116 251 383 596.3 0 383 252 212 596.3 0 212 253 108 596.3 0 108 254 120 596.3 0 120 255 162 596.3 0 162 256 138 596.3 0 138 257 366 596.3 0 366 258 317 596.3 0 317 259 1121 596.3 0 1121 260 5931 596.3 0 5931 261 1764 596.3 0 1764 262 176 596.3 0 176 263 163 596.3 0 163 264 715 596.3 0 715 265 3110 596.3 0 3110 266 2034 596.3 0 2034 267 1502 596.3 0 1502 268 24 596.3 0 24 269 30 596.3 0 30 270 50 596.3 0 50 271 44 596.3 0 44 272 33 596.3 0 33 273 25 596.3 0 25 274 47 596.3 0 47 275 73 596.3 0 73 276 78 596.3 0 78 277 70 596.3 0 70 278 92 596.3 0 92 279 47 596.3 0 47 280 24 596.3 0 24 281 40 596.3 0 40 282 28 596.3 0 28 283 25 596.3 0 25 284 33 596.3 0 33 285 38 596.3 0 38 286 52 596.3 0 52 287 68 596.3 0 68 288 59 596.3 0 59 289 51 596.3 0 51 290 84 596.3 0 84 291 738 596.3 0 738 292 1393 596.3 0 1393 293 1312 596.3 0 1312 294 368 596.3 0 368 295 92 596.3 0 92 296 95 596.3 0 95 297 229 596.3 0 229 298 109 596.3 0 109 299 111 596.3 0 111 300 236 596.3 0 236 301 106 596.3 0 106 Finished in 1.50 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 42,524 Reads with adapters: 2,709 (6.4%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 2,705 (6.4%) Total basepairs processed: 6,458,844 bp Total written (filtered): 734,553 bp (11.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2709 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 34 0.0 3 0 33 1 20 2630 0.0 4 2563 61 5 1 21 16 0.0 4 10 6 22 6 0.0 4 5 1 23 4 0.0 4 0 0 0 4 27 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 127 8 0.0 4 0 0 0 0 8 130 3 0.0 4 0 0 0 0 3 175 1 0.0 4 0 0 0 0 1 186 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 2,705 Reads with adapters: 504 (18.6%) Reads written (passing filters): 2,705 (100.0%) Total basepairs processed: 734,553 bp Total written (filtered): 729,279 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 504 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.0% G: 94.0% T: 1.8% none/other: 0.2% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 315 0.0 0 7 308 10 161 0.0 1 9 152 11 25 0.0 1 1 24 13 1 0.0 1 0 1 269 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.