This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.78 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 215,622 (8.8%) Reads that were too short: 138,074 (5.7%) Reads written (passing filters): 77,548 (3.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 17,175,406 bp (2.3%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 215622 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 330 596.3 0 330 7 3214 596.3 0 3214 8 2643 596.3 0 2643 9 47 596.3 0 47 10 32 596.3 0 32 11 19 596.3 0 19 12 23 596.3 0 23 13 23 596.3 0 23 14 33 596.3 0 33 15 29 596.3 0 29 16 16 596.3 0 16 17 34 596.3 0 34 18 25 596.3 0 25 19 18 596.3 0 18 20 24 596.3 0 24 21 46 596.3 0 46 22 21 596.3 0 21 23 25 596.3 0 25 24 19 596.3 0 19 25 26 596.3 0 26 26 20 596.3 0 20 27 15 596.3 0 15 28 23 596.3 0 23 29 38 596.3 0 38 30 57 596.3 0 57 31 25 596.3 0 25 32 20 596.3 0 20 33 29 596.3 0 29 34 10 596.3 0 10 35 24 596.3 0 24 36 14 596.3 0 14 37 15 596.3 0 15 38 14 596.3 0 14 39 21 596.3 0 21 40 30 596.3 0 30 41 15 596.3 0 15 42 21 596.3 0 21 43 34 596.3 0 34 44 28 596.3 0 28 45 21 596.3 0 21 46 32 596.3 0 32 47 43 596.3 0 43 48 48 596.3 0 48 49 56 596.3 0 56 50 70 596.3 0 70 51 109 596.3 0 109 52 66 596.3 0 66 53 34 596.3 0 34 54 41 596.3 0 41 55 46 596.3 0 46 56 93 596.3 0 93 57 50 596.3 0 50 58 47 596.3 0 47 59 50 596.3 0 50 60 91 596.3 0 91 61 160 596.3 0 160 62 407 596.3 0 407 63 78 596.3 0 78 64 168 596.3 0 168 65 340 596.3 0 340 66 110 596.3 0 110 67 85 596.3 0 85 68 230 596.3 0 230 69 209 596.3 0 209 70 313 596.3 0 313 71 414 596.3 0 414 72 483 596.3 0 483 73 738 596.3 0 738 74 1026 596.3 0 1026 75 18891 596.3 0 18891 76 25280 596.3 0 25280 77 11243 596.3 0 11243 78 51 596.3 0 51 79 46 596.3 0 46 80 33 596.3 0 33 81 32 596.3 0 32 82 19 596.3 0 19 83 30 596.3 0 30 84 19 596.3 0 19 85 21 596.3 0 21 86 18 596.3 0 18 87 14 596.3 0 14 88 35 596.3 0 35 89 24 596.3 0 24 90 39 596.3 0 39 91 43 596.3 0 43 92 38 596.3 0 38 93 9 596.3 0 9 94 15 596.3 0 15 95 35 596.3 0 35 96 13 596.3 0 13 97 10 596.3 0 10 98 16 596.3 0 16 99 16 596.3 0 16 100 14 596.3 0 14 101 13 596.3 0 13 102 8 596.3 0 8 103 15 596.3 0 15 104 13 596.3 0 13 105 16 596.3 0 16 106 27 596.3 0 27 107 12 596.3 0 12 108 17 596.3 0 17 109 20 596.3 0 20 110 22 596.3 0 22 111 16 596.3 0 16 112 12 596.3 0 12 113 25 596.3 0 25 114 30 596.3 0 30 115 17 596.3 0 17 116 26 596.3 0 26 117 27 596.3 0 27 118 24 596.3 0 24 119 52 596.3 0 52 120 32 596.3 0 32 121 25 596.3 0 25 122 23 596.3 0 23 123 24 596.3 0 24 124 23 596.3 0 23 125 124 596.3 0 124 126 135 596.3 0 135 127 195 596.3 0 195 128 83 596.3 0 83 129 84 596.3 0 84 130 28 596.3 0 28 131 48 596.3 0 48 132 266 596.3 0 266 133 228 596.3 0 228 134 46 596.3 0 46 135 235 596.3 0 235 136 395 596.3 0 395 137 252 596.3 0 252 138 39 596.3 0 39 139 15 596.3 0 15 140 32 596.3 0 32 141 47 596.3 0 47 142 81 596.3 0 81 143 32 596.3 0 32 144 134 596.3 0 134 145 65 596.3 0 65 146 19 596.3 0 19 147 29 596.3 0 29 148 29 596.3 0 29 149 18 596.3 0 18 150 21 596.3 0 21 151 18 596.3 0 18 152 33 596.3 0 33 153 28 596.3 0 28 154 28 596.3 0 28 155 38 596.3 0 38 156 45 596.3 0 45 157 79 596.3 0 79 158 32 596.3 0 32 159 13 596.3 0 13 160 14 596.3 0 14 161 55 596.3 0 55 162 76 596.3 0 76 163 85 596.3 0 85 164 41 596.3 0 41 165 30 596.3 0 30 166 67 596.3 0 67 167 110 596.3 0 110 168 172 596.3 0 172 169 80 596.3 0 80 170 22 596.3 0 22 171 24 596.3 0 24 172 27 596.3 0 27 173 29 596.3 0 29 174 26 596.3 0 26 175 28 596.3 0 28 176 31 596.3 0 31 177 33 596.3 0 33 178 28 596.3 0 28 179 31 596.3 0 31 180 58 596.3 0 58 181 74 596.3 0 74 182 131 596.3 0 131 183 80 596.3 0 80 184 85 596.3 0 85 185 56 596.3 0 56 186 164 596.3 0 164 187 175 596.3 0 175 188 266 596.3 0 266 189 108 596.3 0 108 190 61 596.3 0 61 191 64 596.3 0 64 192 82 596.3 0 82 193 262 596.3 0 262 194 396 596.3 0 396 195 343 596.3 0 343 196 176 596.3 0 176 197 393 596.3 0 393 198 480 596.3 0 480 199 473 596.3 0 473 200 188 596.3 0 188 201 149 596.3 0 149 202 91 596.3 0 91 203 188 596.3 0 188 204 178 596.3 0 178 205 406 596.3 0 406 206 70 596.3 0 70 207 60 596.3 0 60 208 79 596.3 0 79 209 56 596.3 0 56 210 87 596.3 0 87 211 158 596.3 0 158 212 79 596.3 0 79 213 157 596.3 0 157 214 211 596.3 0 211 215 163 596.3 0 163 216 143 596.3 0 143 217 232 596.3 0 232 218 386 596.3 0 386 219 289 596.3 0 289 220 231 596.3 0 231 221 218 596.3 0 218 222 131 596.3 0 131 223 283 596.3 0 283 224 532 596.3 0 532 225 523 596.3 0 523 226 278 596.3 0 278 227 123 596.3 0 123 228 148 596.3 0 148 229 132 596.3 0 132 230 116 596.3 0 116 231 157 596.3 0 157 232 200 596.3 0 200 233 331 596.3 0 331 234 555 596.3 0 555 235 760 596.3 0 760 236 983 596.3 0 983 237 1498 596.3 0 1498 238 21874 596.3 0 21874 239 6407 596.3 0 6407 240 20159 596.3 0 20159 241 368 596.3 0 368 242 263 596.3 0 263 243 359 596.3 0 359 244 385 596.3 0 385 245 448 596.3 0 448 246 556 596.3 0 556 247 905 596.3 0 905 248 6805 596.3 0 6805 249 16924 596.3 0 16924 250 19186 596.3 0 19186 251 16402 596.3 0 16402 252 281 596.3 0 281 253 190 596.3 0 190 254 153 596.3 0 153 255 160 596.3 0 160 256 150 596.3 0 150 257 131 596.3 0 131 258 55 596.3 0 55 259 57 596.3 0 57 260 108 596.3 0 108 261 114 596.3 0 114 262 133 596.3 0 133 263 114 596.3 0 114 264 84 596.3 0 84 265 83 596.3 0 83 266 101 596.3 0 101 267 60 596.3 0 60 268 145 596.3 0 145 269 138 596.3 0 138 270 125 596.3 0 125 271 157 596.3 0 157 272 186 596.3 0 186 273 129 596.3 0 129 274 78 596.3 0 78 275 82 596.3 0 82 276 73 596.3 0 73 277 152 596.3 0 152 278 138 596.3 0 138 279 355 596.3 0 355 280 534 596.3 0 534 281 363 596.3 0 363 282 1320 596.3 0 1320 283 1831 596.3 0 1831 284 2640 596.3 0 2640 285 873 596.3 0 873 286 298 596.3 0 298 287 311 596.3 0 311 288 276 596.3 0 276 289 174 596.3 0 174 290 110 596.3 0 110 291 145 596.3 0 145 292 213 596.3 0 213 293 161 596.3 0 161 294 323 596.3 0 323 295 435 596.3 0 435 296 842 596.3 0 842 297 712 596.3 0 712 298 393 596.3 0 393 299 470 596.3 0 470 300 333 596.3 0 333 301 312 596.3 0 312 Finished in 3.09 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 77,548 Reads with adapters: 5,874 (7.6%) Reads that were too short: 330 (0.4%) Reads written (passing filters): 5,544 (7.1%) Total basepairs processed: 17,175,406 bp Total written (filtered): 1,166,552 bp (6.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5874 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 33 0.0 3 0 25 6 2 17 3049 0.0 3 2812 198 32 7 18 15 0.0 3 4 7 2 2 19 15 0.0 3 0 0 11 4 20 10 0.0 3 1 0 0 9 22 1 0.0 3 0 0 0 1 25 7 0.0 3 0 0 0 7 26 1 0.0 3 0 0 0 1 27 3 0.0 3 0 0 0 3 28 2 0.0 3 0 0 0 2 31 11 0.0 3 0 0 1 10 32 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 7 0.0 3 0 0 0 7 47 12 0.0 3 0 0 0 12 48 6 0.0 3 0 0 0 6 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 52 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 7 0.0 3 0 0 1 6 60 12 0.0 3 0 0 1 11 61 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 5 0.0 3 0 0 0 5 65 5 0.0 3 0 0 0 5 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 76 2 0.0 3 0 0 0 2 77 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 6 0.0 3 0 0 0 6 87 2 0.0 3 0 0 1 1 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 1 2 90 5 0.0 3 0 0 1 4 91 101 0.0 3 0 0 1 100 92 39 0.0 3 0 0 0 39 93 5 0.0 3 0 0 1 4 94 11 0.0 3 0 0 0 11 95 3 0.0 3 0 0 0 3 96 256 0.0 3 0 0 2 254 97 10 0.0 3 0 0 0 10 98 998 0.0 3 0 0 16 982 99 261 0.0 3 0 0 3 258 100 12 0.0 3 0 0 0 12 101 89 0.0 3 0 0 2 87 102 24 0.0 3 0 0 0 24 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 4 0.0 3 0 0 0 4 108 4 0.0 3 0 0 0 4 109 27 0.0 3 0 0 1 26 110 1 0.0 3 0 0 0 1 111 10 0.0 3 0 0 0 10 112 6 0.0 3 0 0 2 4 113 3 0.0 3 0 0 0 3 114 4 0.0 3 0 0 0 4 115 32 0.0 3 0 0 0 32 116 218 0.0 3 0 0 1 217 117 68 0.0 3 0 0 1 67 118 6 0.0 3 0 0 0 6 119 35 0.0 3 0 0 2 33 120 18 0.0 3 0 0 1 17 121 5 0.0 3 0 0 0 5 122 15 0.0 3 0 0 1 14 123 7 0.0 3 0 0 0 7 124 52 0.0 3 0 0 0 52 125 5 0.0 3 0 0 0 5 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 7 0.0 3 0 0 0 7 145 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 149 24 0.0 3 0 0 2 22 150 6 0.0 3 0 0 1 5 151 6 0.0 3 0 0 1 5 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 166 5 0.0 3 0 0 0 5 167 10 0.0 3 0 0 0 10 168 6 0.0 3 0 0 0 6 169 1 0.0 3 0 0 0 1 170 4 0.0 3 0 0 0 4 175 2 0.0 3 0 0 0 2 176 14 0.0 3 0 0 0 14 177 8 0.0 3 0 0 0 8 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 2 0.0 3 0 0 0 2 194 4 0.0 3 0 0 0 4 195 4 0.0 3 0 0 0 4 196 7 0.0 3 0 0 0 7 197 3 0.0 3 0 0 0 3 198 2 0.0 3 0 0 0 2 199 3 0.0 3 0 0 0 3 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 4 0.0 3 0 0 0 4 204 6 0.0 3 0 0 0 6 205 1 0.0 3 0 0 1 206 6 0.0 3 0 0 0 6 207 6 0.0 3 0 0 1 5 208 3 0.0 3 0 0 0 3 209 2 0.0 3 0 0 0 2 210 4 0.0 3 0 0 0 4 211 4 0.0 3 0 0 1 3 212 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 214 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 12 0.0 3 0 0 1 11 218 23 0.0 3 0 0 0 23 219 1 0.0 3 0 0 1 220 5 0.0 3 0 0 0 5 221 3 0.0 3 0 0 0 3 222 4 0.0 3 0 0 0 4 224 1 0.0 3 0 0 0 1 226 5 0.0 3 0 0 0 5 228 2 0.0 3 0 0 0 2 229 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 5,544 Reads with adapters: 36 (0.6%) Reads written (passing filters): 5,544 (100.0%) Total basepairs processed: 1,166,552 bp Total written (filtered): 1,166,443 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 36 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 30.6% G: 19.4% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 35 86.6 0 35 4 1 21.7 0 1