This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.58 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 56,934 (2.3%) Reads that were too short: 17,314 (0.7%) Reads written (passing filters): 39,620 (1.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 7,106,830 bp (1.0%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 56934 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 189 596.3 0 189 7 654 596.3 0 654 8 2776 596.3 0 2776 9 2397 596.3 0 2397 10 30 596.3 0 30 11 42 596.3 0 42 12 29 596.3 0 29 13 17 596.3 0 17 14 24 596.3 0 24 15 24 596.3 0 24 16 22 596.3 0 22 17 19 596.3 0 19 18 27 596.3 0 27 19 22 596.3 0 22 20 32 596.3 0 32 21 33 596.3 0 33 22 20 596.3 0 20 23 24 596.3 0 24 24 22 596.3 0 22 25 23 596.3 0 23 26 26 596.3 0 26 27 33 596.3 0 33 28 26 596.3 0 26 29 48 596.3 0 48 30 24 596.3 0 24 31 29 596.3 0 29 32 8 596.3 0 8 33 20 596.3 0 20 34 31 596.3 0 31 35 30 596.3 0 30 36 42 596.3 0 42 37 24 596.3 0 24 38 30 596.3 0 30 39 33 596.3 0 33 40 35 596.3 0 35 41 31 596.3 0 31 42 20 596.3 0 20 43 30 596.3 0 30 44 28 596.3 0 28 45 32 596.3 0 32 46 55 596.3 0 55 47 22 596.3 0 22 48 28 596.3 0 28 49 43 596.3 0 43 50 34 596.3 0 34 51 27 596.3 0 27 52 27 596.3 0 27 53 91 596.3 0 91 54 53 596.3 0 53 55 114 596.3 0 114 56 31 596.3 0 31 57 29 596.3 0 29 58 53 596.3 0 53 59 49 596.3 0 49 60 31 596.3 0 31 61 54 596.3 0 54 62 38 596.3 0 38 63 123 596.3 0 123 64 92 596.3 0 92 65 126 596.3 0 126 66 29 596.3 0 29 67 50 596.3 0 50 68 85 596.3 0 85 69 124 596.3 0 124 70 50 596.3 0 50 71 75 596.3 0 75 72 406 596.3 0 406 73 1545 596.3 0 1545 74 1796 596.3 0 1796 75 1274 596.3 0 1274 76 25 596.3 0 25 77 36 596.3 0 36 78 33 596.3 0 33 79 36 596.3 0 36 80 31 596.3 0 31 81 10 596.3 0 10 82 24 596.3 0 24 83 27 596.3 0 27 84 36 596.3 0 36 85 41 596.3 0 41 86 61 596.3 0 61 87 121 596.3 0 121 88 315 596.3 0 315 89 70 596.3 0 70 90 38 596.3 0 38 91 63 596.3 0 63 92 41 596.3 0 41 93 37 596.3 0 37 94 40 596.3 0 40 95 39 596.3 0 39 96 34 596.3 0 34 97 24 596.3 0 24 98 27 596.3 0 27 99 37 596.3 0 37 100 60 596.3 0 60 101 54 596.3 0 54 102 28 596.3 0 28 103 25 596.3 0 25 104 32 596.3 0 32 105 79 596.3 0 79 106 35 596.3 0 35 107 50 596.3 0 50 108 72 596.3 0 72 109 97 596.3 0 97 110 55 596.3 0 55 111 28 596.3 0 28 112 22 596.3 0 22 113 27 596.3 0 27 114 31 596.3 0 31 115 41 596.3 0 41 116 28 596.3 0 28 117 26 596.3 0 26 118 33 596.3 0 33 119 25 596.3 0 25 120 28 596.3 0 28 121 44 596.3 0 44 122 43 596.3 0 43 123 22 596.3 0 22 124 27 596.3 0 27 125 31 596.3 0 31 126 35 596.3 0 35 127 37 596.3 0 37 128 68 596.3 0 68 129 64 596.3 0 64 130 88 596.3 0 88 131 52 596.3 0 52 132 52 596.3 0 52 133 52 596.3 0 52 134 35 596.3 0 35 135 73 596.3 0 73 136 297 596.3 0 297 137 157 596.3 0 157 138 63 596.3 0 63 139 39 596.3 0 39 140 37 596.3 0 37 141 87 596.3 0 87 142 93 596.3 0 93 143 102 596.3 0 102 144 64 596.3 0 64 145 129 596.3 0 129 146 1133 596.3 0 1133 147 282 596.3 0 282 148 405 596.3 0 405 149 113 596.3 0 113 150 143 596.3 0 143 151 82 596.3 0 82 152 84 596.3 0 84 153 85 596.3 0 85 154 220 596.3 0 220 155 166 596.3 0 166 156 229 596.3 0 229 157 285 596.3 0 285 158 628 596.3 0 628 159 390 596.3 0 390 160 629 596.3 0 629 161 338 596.3 0 338 162 260 596.3 0 260 163 95 596.3 0 95 164 53 596.3 0 53 165 81 596.3 0 81 166 148 596.3 0 148 167 257 596.3 0 257 168 149 596.3 0 149 169 146 596.3 0 146 170 161 596.3 0 161 171 484 596.3 0 484 172 153 596.3 0 153 173 267 596.3 0 267 174 2712 596.3 0 2712 175 1730 596.3 0 1730 176 3126 596.3 0 3126 177 209 596.3 0 209 178 76 596.3 0 76 179 56 596.3 0 56 180 150 596.3 0 150 181 695 596.3 0 695 182 360 596.3 0 360 183 105 596.3 0 105 184 88 596.3 0 88 185 190 596.3 0 190 186 386 596.3 0 386 187 2144 596.3 0 2144 188 886 596.3 0 886 189 1064 596.3 0 1064 190 56 596.3 0 56 191 56 596.3 0 56 192 64 596.3 0 64 193 41 596.3 0 41 194 66 596.3 0 66 195 84 596.3 0 84 196 67 596.3 0 67 197 52 596.3 0 52 198 43 596.3 0 43 199 49 596.3 0 49 200 61 596.3 0 61 201 65 596.3 0 65 202 94 596.3 0 94 203 94 596.3 0 94 204 145 596.3 0 145 205 152 596.3 0 152 206 147 596.3 0 147 207 74 596.3 0 74 208 56 596.3 0 56 209 71 596.3 0 71 210 46 596.3 0 46 211 40 596.3 0 40 212 42 596.3 0 42 213 60 596.3 0 60 214 69 596.3 0 69 215 78 596.3 0 78 216 71 596.3 0 71 217 78 596.3 0 78 218 71 596.3 0 71 219 67 596.3 0 67 220 87 596.3 0 87 221 68 596.3 0 68 222 66 596.3 0 66 223 87 596.3 0 87 224 73 596.3 0 73 225 73 596.3 0 73 226 60 596.3 0 60 227 214 596.3 0 214 228 138 596.3 0 138 229 310 596.3 0 310 230 70 596.3 0 70 231 103 596.3 0 103 232 128 596.3 0 128 233 112 596.3 0 112 234 86 596.3 0 86 235 80 596.3 0 80 236 121 596.3 0 121 237 168 596.3 0 168 238 149 596.3 0 149 239 204 596.3 0 204 240 116 596.3 0 116 241 96 596.3 0 96 242 86 596.3 0 86 243 80 596.3 0 80 244 99 596.3 0 99 245 66 596.3 0 66 246 95 596.3 0 95 247 117 596.3 0 117 248 259 596.3 0 259 249 304 596.3 0 304 250 217 596.3 0 217 251 416 596.3 0 416 252 299 596.3 0 299 253 443 596.3 0 443 254 161 596.3 0 161 255 148 596.3 0 148 256 105 596.3 0 105 257 74 596.3 0 74 258 76 596.3 0 76 259 127 596.3 0 127 260 281 596.3 0 281 261 539 596.3 0 539 262 633 596.3 0 633 263 455 596.3 0 455 264 254 596.3 0 254 265 77 596.3 0 77 266 68 596.3 0 68 267 73 596.3 0 73 268 166 596.3 0 166 269 214 596.3 0 214 270 162 596.3 0 162 271 122 596.3 0 122 272 195 596.3 0 195 273 142 596.3 0 142 274 148 596.3 0 148 275 86 596.3 0 86 276 79 596.3 0 79 277 151 596.3 0 151 278 216 596.3 0 216 279 278 596.3 0 278 280 188 596.3 0 188 281 412 596.3 0 412 282 403 596.3 0 403 283 343 596.3 0 343 284 143 596.3 0 143 285 293 596.3 0 293 286 225 596.3 0 225 287 127 596.3 0 127 288 112 596.3 0 112 289 105 596.3 0 105 290 214 596.3 0 214 291 329 596.3 0 329 292 310 596.3 0 310 293 252 596.3 0 252 294 180 596.3 0 180 295 290 596.3 0 290 296 178 596.3 0 178 297 327 596.3 0 327 298 316 596.3 0 316 299 340 596.3 0 340 300 374 596.3 0 374 301 278 596.3 0 278 Finished in 1.41 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 39,620 Reads with adapters: 3,456 (8.7%) Reads that were too short: 128 (0.3%) Reads written (passing filters): 3,328 (8.4%) Total basepairs processed: 7,106,830 bp Total written (filtered): 904,085 bp (12.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3456 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 37 0.0 3 0 25 2 10 17 2768 0.0 3 2586 151 24 7 18 11 0.0 3 0 10 0 1 19 418 0.0 3 383 19 13 3 20 10 0.0 3 3 1 0 6 21 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 1 29 2 0.0 3 0 0 0 2 30 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 36 12 0.0 3 0 0 0 12 37 1 0.0 3 0 0 0 1 38 3 0.0 3 0 0 0 3 39 5 0.0 3 0 0 1 4 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 0 4 46 16 0.0 3 0 0 0 16 47 1 0.0 3 0 0 0 1 49 4 0.0 3 0 0 0 4 50 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 5 0.0 3 0 0 0 5 57 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 5 0.0 3 0 0 0 5 85 6 0.0 3 0 0 0 6 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 30 0.0 3 0 0 0 30 108 3 0.0 3 0 0 0 3 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 4 0.0 3 0 0 0 4 115 2 0.0 3 0 0 1 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 1 140 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,328 Reads with adapters: 63 (1.9%) Reads written (passing filters): 3,328 (100.0%) Total basepairs processed: 904,085 bp Total written (filtered): 903,859 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 63 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.5% C: 9.5% G: 66.7% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 52.0 0 26 4 37 13.0 0 37