This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.65 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 47,099 (1.9%) Reads that were too short: 19,457 (0.8%) Reads written (passing filters): 27,642 (1.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 4,700,213 bp (0.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 47099 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 83 596.3 0 83 7 1931 596.3 0 1931 8 1598 596.3 0 1598 9 15 596.3 0 15 10 21 596.3 0 21 11 82 596.3 0 82 12 28 596.3 0 28 13 187 596.3 0 187 14 113 596.3 0 113 15 248 596.3 0 248 16 45 596.3 0 45 17 8 596.3 0 8 18 4 596.3 0 4 19 7 596.3 0 7 20 8 596.3 0 8 21 14 596.3 0 14 22 11 596.3 0 11 23 12 596.3 0 12 24 30 596.3 0 30 25 76 596.3 0 76 26 59 596.3 0 59 27 107 596.3 0 107 28 8 596.3 0 8 29 10 596.3 0 10 30 10 596.3 0 10 31 12 596.3 0 12 32 11 596.3 0 11 33 8 596.3 0 8 34 10 596.3 0 10 35 20 596.3 0 20 36 9 596.3 0 9 37 9 596.3 0 9 38 13 596.3 0 13 39 11 596.3 0 11 40 14 596.3 0 14 41 7 596.3 0 7 42 15 596.3 0 15 43 22 596.3 0 22 44 6 596.3 0 6 45 7 596.3 0 7 46 8 596.3 0 8 47 10 596.3 0 10 48 15 596.3 0 15 49 12 596.3 0 12 50 20 596.3 0 20 51 6 596.3 0 6 52 11 596.3 0 11 53 19 596.3 0 19 54 25 596.3 0 25 55 22 596.3 0 22 56 24 596.3 0 24 57 27 596.3 0 27 58 18 596.3 0 18 59 50 596.3 0 50 60 55 596.3 0 55 61 90 596.3 0 90 62 76 596.3 0 76 63 12 596.3 0 12 64 10 596.3 0 10 65 11 596.3 0 11 66 19 596.3 0 19 67 10 596.3 0 10 68 15 596.3 0 15 69 85 596.3 0 85 70 15 596.3 0 15 71 17 596.3 0 17 72 22 596.3 0 22 73 17 596.3 0 17 74 23 596.3 0 23 75 20 596.3 0 20 76 15 596.3 0 15 77 27 596.3 0 27 78 50 596.3 0 50 79 55 596.3 0 55 80 109 596.3 0 109 81 70 596.3 0 70 82 534 596.3 0 534 83 326 596.3 0 326 84 398 596.3 0 398 85 13 596.3 0 13 86 16 596.3 0 16 87 17 596.3 0 17 88 19 596.3 0 19 89 28 596.3 0 28 90 12 596.3 0 12 91 13 596.3 0 13 92 12 596.3 0 12 93 31 596.3 0 31 94 17 596.3 0 17 95 22 596.3 0 22 96 37 596.3 0 37 97 41 596.3 0 41 98 23 596.3 0 23 99 35 596.3 0 35 100 30 596.3 0 30 101 13 596.3 0 13 102 41 596.3 0 41 103 45 596.3 0 45 104 59 596.3 0 59 105 41 596.3 0 41 106 108 596.3 0 108 107 44 596.3 0 44 108 24 596.3 0 24 109 33 596.3 0 33 110 286 596.3 0 286 111 121 596.3 0 121 112 52 596.3 0 52 113 72 596.3 0 72 114 30 596.3 0 30 115 44 596.3 0 44 116 32 596.3 0 32 117 27 596.3 0 27 118 23 596.3 0 23 119 57 596.3 0 57 120 62 596.3 0 62 121 38 596.3 0 38 122 151 596.3 0 151 123 171 596.3 0 171 124 87 596.3 0 87 125 127 596.3 0 127 126 161 596.3 0 161 127 61 596.3 0 61 128 47 596.3 0 47 129 30 596.3 0 30 130 29 596.3 0 29 131 36 596.3 0 36 132 40 596.3 0 40 133 42 596.3 0 42 134 66 596.3 0 66 135 38 596.3 0 38 136 63 596.3 0 63 137 136 596.3 0 136 138 179 596.3 0 179 139 175 596.3 0 175 140 133 596.3 0 133 141 84 596.3 0 84 142 111 596.3 0 111 143 40 596.3 0 40 144 37 596.3 0 37 145 108 596.3 0 108 146 141 596.3 0 141 147 84 596.3 0 84 148 77 596.3 0 77 149 37 596.3 0 37 150 50 596.3 0 50 151 56 596.3 0 56 152 52 596.3 0 52 153 86 596.3 0 86 154 50 596.3 0 50 155 103 596.3 0 103 156 143 596.3 0 143 157 144 596.3 0 144 158 159 596.3 0 159 159 191 596.3 0 191 160 867 596.3 0 867 161 1356 596.3 0 1356 162 1586 596.3 0 1586 163 543 596.3 0 543 164 119 596.3 0 119 165 62 596.3 0 62 166 82 596.3 0 82 167 63 596.3 0 63 168 74 596.3 0 74 169 80 596.3 0 80 170 87 596.3 0 87 171 37 596.3 0 37 172 62 596.3 0 62 173 71 596.3 0 71 174 122 596.3 0 122 175 106 596.3 0 106 176 93 596.3 0 93 177 95 596.3 0 95 178 85 596.3 0 85 179 106 596.3 0 106 180 345 596.3 0 345 181 758 596.3 0 758 182 835 596.3 0 835 183 526 596.3 0 526 184 156 596.3 0 156 185 164 596.3 0 164 186 242 596.3 0 242 187 2783 596.3 0 2783 188 1072 596.3 0 1072 189 1928 596.3 0 1928 190 67 596.3 0 67 191 69 596.3 0 69 192 35 596.3 0 35 193 41 596.3 0 41 194 50 596.3 0 50 195 52 596.3 0 52 196 78 596.3 0 78 197 65 596.3 0 65 198 59 596.3 0 59 199 102 596.3 0 102 200 74 596.3 0 74 201 65 596.3 0 65 202 92 596.3 0 92 203 152 596.3 0 152 204 178 596.3 0 178 205 232 596.3 0 232 206 425 596.3 0 425 207 255 596.3 0 255 208 92 596.3 0 92 209 62 596.3 0 62 210 389 596.3 0 389 211 792 596.3 0 792 212 1062 596.3 0 1062 213 366 596.3 0 366 214 163 596.3 0 163 215 91 596.3 0 91 216 95 596.3 0 95 217 71 596.3 0 71 218 62 596.3 0 62 219 214 596.3 0 214 220 287 596.3 0 287 221 333 596.3 0 333 222 81 596.3 0 81 223 55 596.3 0 55 224 127 596.3 0 127 225 131 596.3 0 131 226 136 596.3 0 136 227 138 596.3 0 138 228 116 596.3 0 116 229 149 596.3 0 149 230 763 596.3 0 763 231 925 596.3 0 925 232 998 596.3 0 998 233 93 596.3 0 93 234 37 596.3 0 37 235 40 596.3 0 40 236 40 596.3 0 40 237 33 596.3 0 33 238 34 596.3 0 34 239 65 596.3 0 65 240 86 596.3 0 86 241 99 596.3 0 99 242 52 596.3 0 52 243 43 596.3 0 43 244 39 596.3 0 39 245 48 596.3 0 48 246 37 596.3 0 37 247 52 596.3 0 52 248 30 596.3 0 30 249 24 596.3 0 24 250 32 596.3 0 32 251 46 596.3 0 46 252 58 596.3 0 58 253 105 596.3 0 105 254 132 596.3 0 132 255 116 596.3 0 116 256 110 596.3 0 110 257 69 596.3 0 69 258 84 596.3 0 84 259 78 596.3 0 78 260 95 596.3 0 95 261 76 596.3 0 76 262 59 596.3 0 59 263 107 596.3 0 107 264 109 596.3 0 109 265 103 596.3 0 103 266 70 596.3 0 70 267 74 596.3 0 74 268 144 596.3 0 144 269 128 596.3 0 128 270 56 596.3 0 56 271 66 596.3 0 66 272 83 596.3 0 83 273 109 596.3 0 109 274 216 596.3 0 216 275 424 596.3 0 424 276 550 596.3 0 550 277 476 596.3 0 476 278 199 596.3 0 199 279 303 596.3 0 303 280 335 596.3 0 335 281 203 596.3 0 203 282 121 596.3 0 121 283 244 596.3 0 244 284 260 596.3 0 260 285 264 596.3 0 264 286 117 596.3 0 117 287 200 596.3 0 200 288 280 596.3 0 280 289 621 596.3 0 621 290 282 596.3 0 282 291 110 596.3 0 110 292 67 596.3 0 67 293 94 596.3 0 94 294 111 596.3 0 111 295 125 596.3 0 125 296 103 596.3 0 103 297 113 596.3 0 113 298 184 596.3 0 184 299 80 596.3 0 80 300 354 596.3 0 354 301 1028 596.3 0 1028 Finished in 0.95 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 27,642 Reads with adapters: 2,147 (7.8%) Reads that were too short: 132 (0.5%) Reads written (passing filters): 2,015 (7.3%) Total basepairs processed: 4,700,213 bp Total written (filtered): 541,544 bp (11.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2147 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 1 5 16 21 0.0 3 0 20 1 17 1862 0.0 3 1725 110 25 2 18 14 0.0 3 1 11 0 2 19 8 0.0 3 0 1 6 1 20 4 0.0 3 1 0 0 3 22 5 0.0 3 0 0 0 5 25 1 0.0 3 0 0 0 1 27 3 0.0 3 0 0 0 3 33 4 0.0 3 0 0 0 4 34 2 0.0 3 0 0 0 2 35 3 0.0 3 0 0 0 3 36 2 0.0 3 0 0 0 2 37 12 0.0 3 0 0 0 12 39 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 0 4 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 15 0.0 3 0 0 0 15 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 1 62 6 0.0 3 0 0 1 5 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 6 0.0 3 0 0 0 6 70 2 0.0 3 0 0 0 2 71 4 0.0 3 0 0 0 4 72 1 0.0 3 0 0 1 73 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 1 76 3 0.0 3 0 0 0 3 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 7 0.0 3 0 0 1 6 113 5 0.0 3 0 0 0 5 114 2 0.0 3 0 0 0 2 116 1 0.0 3 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 13 0.0 3 0 0 1 12 124 5 0.0 3 0 0 0 5 125 4 0.0 3 0 0 1 3 126 6 0.0 3 0 0 1 5 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 6 0.0 3 0 0 0 6 133 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 1 1 140 1 0.0 3 0 0 0 1 141 36 0.0 3 0 0 0 36 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 2,015 Reads with adapters: 30 (1.5%) Reads written (passing filters): 2,015 (100.0%) Total basepairs processed: 541,544 bp Total written (filtered): 541,454 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 30 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.7% C: 73.3% G: 16.7% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 30 31.5 0 30