This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.16 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 31,117 (1.3%) Reads that were too short: 9,705 (0.4%) Reads written (passing filters): 21,412 (0.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 3,872,130 bp (0.5%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 31117 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 51 596.3 0 51 7 1685 596.3 0 1685 8 1987 596.3 0 1987 9 5 596.3 0 5 10 10 596.3 0 10 11 35 596.3 0 35 12 8 596.3 0 8 13 104 596.3 0 104 14 65 596.3 0 65 15 118 596.3 0 118 16 36 596.3 0 36 17 4 596.3 0 4 18 6 596.3 0 6 19 6 596.3 0 6 20 4 596.3 0 4 21 6 596.3 0 6 22 5 596.3 0 5 23 11 596.3 0 11 24 9 596.3 0 9 25 492 596.3 0 492 26 109 596.3 0 109 27 6 596.3 0 6 28 9 596.3 0 9 29 8 596.3 0 8 30 4 596.3 0 4 31 10 596.3 0 10 32 4 596.3 0 4 33 11 596.3 0 11 34 5 596.3 0 5 35 5 596.3 0 5 36 2 596.3 0 2 37 9 596.3 0 9 38 5 596.3 0 5 39 4 596.3 0 4 40 9 596.3 0 9 41 7 596.3 0 7 42 7 596.3 0 7 43 9 596.3 0 9 44 14 596.3 0 14 45 7 596.3 0 7 46 7 596.3 0 7 47 16 596.3 0 16 48 8 596.3 0 8 49 6 596.3 0 6 50 8 596.3 0 8 51 8 596.3 0 8 52 7 596.3 0 7 53 21 596.3 0 21 54 23 596.3 0 23 55 20 596.3 0 20 56 20 596.3 0 20 57 20 596.3 0 20 58 13 596.3 0 13 59 47 596.3 0 47 60 30 596.3 0 30 61 54 596.3 0 54 62 19 596.3 0 19 63 8 596.3 0 8 64 11 596.3 0 11 65 15 596.3 0 15 66 11 596.3 0 11 67 10 596.3 0 10 68 19 596.3 0 19 69 121 596.3 0 121 70 19 596.3 0 19 71 14 596.3 0 14 72 13 596.3 0 13 73 8 596.3 0 8 74 12 596.3 0 12 75 13 596.3 0 13 76 11 596.3 0 11 77 14 596.3 0 14 78 24 596.3 0 24 79 20 596.3 0 20 80 35 596.3 0 35 81 92 596.3 0 92 82 861 596.3 0 861 83 565 596.3 0 565 84 778 596.3 0 778 85 16 596.3 0 16 86 26 596.3 0 26 87 27 596.3 0 27 88 21 596.3 0 21 89 35 596.3 0 35 90 24 596.3 0 24 91 19 596.3 0 19 92 11 596.3 0 11 93 23 596.3 0 23 94 23 596.3 0 23 95 24 596.3 0 24 96 20 596.3 0 20 97 26 596.3 0 26 98 23 596.3 0 23 99 14 596.3 0 14 100 25 596.3 0 25 101 29 596.3 0 29 102 27 596.3 0 27 103 28 596.3 0 28 104 28 596.3 0 28 105 37 596.3 0 37 106 112 596.3 0 112 107 27 596.3 0 27 108 31 596.3 0 31 109 21 596.3 0 21 110 293 596.3 0 293 111 100 596.3 0 100 112 36 596.3 0 36 113 53 596.3 0 53 114 29 596.3 0 29 115 35 596.3 0 35 116 22 596.3 0 22 117 21 596.3 0 21 118 30 596.3 0 30 119 42 596.3 0 42 120 47 596.3 0 47 121 19 596.3 0 19 122 82 596.3 0 82 123 106 596.3 0 106 124 44 596.3 0 44 125 80 596.3 0 80 126 121 596.3 0 121 127 44 596.3 0 44 128 43 596.3 0 43 129 34 596.3 0 34 130 22 596.3 0 22 131 26 596.3 0 26 132 26 596.3 0 26 133 35 596.3 0 35 134 37 596.3 0 37 135 28 596.3 0 28 136 35 596.3 0 35 137 52 596.3 0 52 138 70 596.3 0 70 139 77 596.3 0 77 140 65 596.3 0 65 141 58 596.3 0 58 142 50 596.3 0 50 143 34 596.3 0 34 144 41 596.3 0 41 145 116 596.3 0 116 146 94 596.3 0 94 147 35 596.3 0 35 148 33 596.3 0 33 149 24 596.3 0 24 150 39 596.3 0 39 151 28 596.3 0 28 152 25 596.3 0 25 153 67 596.3 0 67 154 35 596.3 0 35 155 35 596.3 0 35 156 46 596.3 0 46 157 62 596.3 0 62 158 49 596.3 0 49 159 84 596.3 0 84 160 319 596.3 0 319 161 255 596.3 0 255 162 488 596.3 0 488 163 108 596.3 0 108 164 44 596.3 0 44 165 41 596.3 0 41 166 36 596.3 0 36 167 52 596.3 0 52 168 48 596.3 0 48 169 66 596.3 0 66 170 72 596.3 0 72 171 32 596.3 0 32 172 46 596.3 0 46 173 43 596.3 0 43 174 72 596.3 0 72 175 54 596.3 0 54 176 43 596.3 0 43 177 59 596.3 0 59 178 51 596.3 0 51 179 46 596.3 0 46 180 101 596.3 0 101 181 157 596.3 0 157 182 228 596.3 0 228 183 143 596.3 0 143 184 74 596.3 0 74 185 125 596.3 0 125 186 164 596.3 0 164 187 3246 596.3 0 3246 188 1139 596.3 0 1139 189 2236 596.3 0 2236 190 79 596.3 0 79 191 43 596.3 0 43 192 37 596.3 0 37 193 39 596.3 0 39 194 24 596.3 0 24 195 40 596.3 0 40 196 36 596.3 0 36 197 42 596.3 0 42 198 40 596.3 0 40 199 55 596.3 0 55 200 38 596.3 0 38 201 53 596.3 0 53 202 40 596.3 0 40 203 51 596.3 0 51 204 63 596.3 0 63 205 77 596.3 0 77 206 123 596.3 0 123 207 96 596.3 0 96 208 51 596.3 0 51 209 34 596.3 0 34 210 103 596.3 0 103 211 200 596.3 0 200 212 326 596.3 0 326 213 164 596.3 0 164 214 64 596.3 0 64 215 36 596.3 0 36 216 47 596.3 0 47 217 33 596.3 0 33 218 50 596.3 0 50 219 128 596.3 0 128 220 178 596.3 0 178 221 232 596.3 0 232 222 46 596.3 0 46 223 45 596.3 0 45 224 87 596.3 0 87 225 61 596.3 0 61 226 57 596.3 0 57 227 91 596.3 0 91 228 65 596.3 0 65 229 68 596.3 0 68 230 313 596.3 0 313 231 379 596.3 0 379 232 393 596.3 0 393 233 78 596.3 0 78 234 21 596.3 0 21 235 22 596.3 0 22 236 26 596.3 0 26 237 34 596.3 0 34 238 32 596.3 0 32 239 48 596.3 0 48 240 58 596.3 0 58 241 72 596.3 0 72 242 40 596.3 0 40 243 41 596.3 0 41 244 27 596.3 0 27 245 29 596.3 0 29 246 29 596.3 0 29 247 28 596.3 0 28 248 25 596.3 0 25 249 16 596.3 0 16 250 25 596.3 0 25 251 30 596.3 0 30 252 47 596.3 0 47 253 57 596.3 0 57 254 96 596.3 0 96 255 65 596.3 0 65 256 46 596.3 0 46 257 42 596.3 0 42 258 44 596.3 0 44 259 48 596.3 0 48 260 49 596.3 0 49 261 49 596.3 0 49 262 35 596.3 0 35 263 59 596.3 0 59 264 58 596.3 0 58 265 87 596.3 0 87 266 44 596.3 0 44 267 39 596.3 0 39 268 62 596.3 0 62 269 60 596.3 0 60 270 31 596.3 0 31 271 54 596.3 0 54 272 46 596.3 0 46 273 61 596.3 0 61 274 121 596.3 0 121 275 294 596.3 0 294 276 356 596.3 0 356 277 379 596.3 0 379 278 127 596.3 0 127 279 155 596.3 0 155 280 188 596.3 0 188 281 112 596.3 0 112 282 60 596.3 0 60 283 106 596.3 0 106 284 86 596.3 0 86 285 99 596.3 0 99 286 44 596.3 0 44 287 91 596.3 0 91 288 144 596.3 0 144 289 370 596.3 0 370 290 207 596.3 0 207 291 83 596.3 0 83 292 69 596.3 0 69 293 68 596.3 0 68 294 71 596.3 0 71 295 77 596.3 0 77 296 46 596.3 0 46 297 151 596.3 0 151 298 167 596.3 0 167 299 55 596.3 0 55 300 223 596.3 0 223 301 195 596.3 0 195 Finished in 0.83 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 21,412 Reads with adapters: 2,062 (9.6%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 2,053 (9.6%) Total basepairs processed: 3,872,130 bp Total written (filtered): 558,672 bp (14.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2062 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 25 0.0 3 0 25 20 2001 0.0 4 1963 38 21 5 0.0 4 4 1 22 1 0.0 4 0 0 1 23 4 0.0 4 2 0 0 2 30 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 11 0.0 4 0 0 0 0 11 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 2,053 Reads with adapters: 171 (8.3%) Reads written (passing filters): 2,053 (100.0%) Total basepairs processed: 558,672 bp Total written (filtered): 556,626 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 171 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 2.3% G: 95.3% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 46 0.0 1 2 44 11 121 0.0 1 1 120 13 1 0.0 1 0 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.