This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.88 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 83,625 (3.4%) Reads that were too short: 30,903 (1.3%) Reads written (passing filters): 52,722 (2.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 8,366,201 bp (1.1%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 83625 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 187 596.3 0 187 7 801 596.3 0 801 8 60 596.3 0 60 9 957 596.3 0 957 10 1094 596.3 0 1094 11 23 596.3 0 23 12 15 596.3 0 15 13 31 596.3 0 31 14 20 596.3 0 20 15 17 596.3 0 17 16 19 596.3 0 19 17 11 596.3 0 11 18 11 596.3 0 11 19 18 596.3 0 18 20 13 596.3 0 13 21 13 596.3 0 13 22 16 596.3 0 16 23 11 596.3 0 11 24 15 596.3 0 15 25 19 596.3 0 19 26 18 596.3 0 18 27 29 596.3 0 29 28 24 596.3 0 24 29 21 596.3 0 21 30 23 596.3 0 23 31 15 596.3 0 15 32 16 596.3 0 16 33 29 596.3 0 29 34 10 596.3 0 10 35 20 596.3 0 20 36 33 596.3 0 33 37 23 596.3 0 23 38 102 596.3 0 102 39 77 596.3 0 77 40 84 596.3 0 84 41 29 596.3 0 29 42 16 596.3 0 16 43 32 596.3 0 32 44 20 596.3 0 20 45 31 596.3 0 31 46 19 596.3 0 19 47 21 596.3 0 21 48 13 596.3 0 13 49 22 596.3 0 22 50 34 596.3 0 34 51 17 596.3 0 17 52 21 596.3 0 21 53 206 596.3 0 206 54 97 596.3 0 97 55 165 596.3 0 165 56 41 596.3 0 41 57 198 596.3 0 198 58 28 596.3 0 28 59 18 596.3 0 18 60 16 596.3 0 16 61 14 596.3 0 14 62 17 596.3 0 17 63 23 596.3 0 23 64 23 596.3 0 23 65 33 596.3 0 33 66 175 596.3 0 175 67 69 596.3 0 69 68 109 596.3 0 109 69 118 596.3 0 118 70 37 596.3 0 37 71 70 596.3 0 70 72 115 596.3 0 115 73 39 596.3 0 39 74 48 596.3 0 48 75 22 596.3 0 22 76 11 596.3 0 11 77 16 596.3 0 16 78 18 596.3 0 18 79 21 596.3 0 21 80 20 596.3 0 20 81 32 596.3 0 32 82 25 596.3 0 25 83 25 596.3 0 25 84 24 596.3 0 24 85 20 596.3 0 20 86 56 596.3 0 56 87 36 596.3 0 36 88 33 596.3 0 33 89 27 596.3 0 27 90 25 596.3 0 25 91 67 596.3 0 67 92 24 596.3 0 24 93 50 596.3 0 50 94 52 596.3 0 52 95 64 596.3 0 64 96 23 596.3 0 23 97 23 596.3 0 23 98 28 596.3 0 28 99 33 596.3 0 33 100 18 596.3 0 18 101 34 596.3 0 34 102 45 596.3 0 45 103 128 596.3 0 128 104 150 596.3 0 150 105 176 596.3 0 176 106 65 596.3 0 65 107 60 596.3 0 60 108 58 596.3 0 58 109 47 596.3 0 47 110 29 596.3 0 29 111 97 596.3 0 97 112 96 596.3 0 96 113 191 596.3 0 191 114 55 596.3 0 55 115 61 596.3 0 61 116 40 596.3 0 40 117 46 596.3 0 46 118 34 596.3 0 34 119 53 596.3 0 53 120 83 596.3 0 83 121 76 596.3 0 76 122 65 596.3 0 65 123 144 596.3 0 144 124 198 596.3 0 198 125 2018 596.3 0 2018 126 1093 596.3 0 1093 127 2591 596.3 0 2591 128 87 596.3 0 87 129 40 596.3 0 40 130 71 596.3 0 71 131 77 596.3 0 77 132 98 596.3 0 98 133 115 596.3 0 115 134 102 596.3 0 102 135 103 596.3 0 103 136 60 596.3 0 60 137 82 596.3 0 82 138 66 596.3 0 66 139 93 596.3 0 93 140 128 596.3 0 128 141 75 596.3 0 75 142 88 596.3 0 88 143 91 596.3 0 91 144 207 596.3 0 207 145 520 596.3 0 520 146 379 596.3 0 379 147 227 596.3 0 227 148 124 596.3 0 124 149 166 596.3 0 166 150 367 596.3 0 367 151 303 596.3 0 303 152 502 596.3 0 502 153 163 596.3 0 163 154 318 596.3 0 318 155 611 596.3 0 611 156 1512 596.3 0 1512 157 1633 596.3 0 1633 158 606 596.3 0 606 159 738 596.3 0 738 160 5400 596.3 0 5400 161 4632 596.3 0 4632 162 5940 596.3 0 5940 163 317 596.3 0 317 164 217 596.3 0 217 165 119 596.3 0 119 166 219 596.3 0 219 167 102 596.3 0 102 168 109 596.3 0 109 169 384 596.3 0 384 170 132 596.3 0 132 171 218 596.3 0 218 172 87 596.3 0 87 173 131 596.3 0 131 174 127 596.3 0 127 175 555 596.3 0 555 176 838 596.3 0 838 177 736 596.3 0 736 178 151 596.3 0 151 179 201 596.3 0 201 180 1287 596.3 0 1287 181 2001 596.3 0 2001 182 2354 596.3 0 2354 183 882 596.3 0 882 184 80 596.3 0 80 185 116 596.3 0 116 186 128 596.3 0 128 187 119 596.3 0 119 188 87 596.3 0 87 189 76 596.3 0 76 190 69 596.3 0 69 191 90 596.3 0 90 192 55 596.3 0 55 193 50 596.3 0 50 194 65 596.3 0 65 195 63 596.3 0 63 196 49 596.3 0 49 197 84 596.3 0 84 198 92 596.3 0 92 199 92 596.3 0 92 200 111 596.3 0 111 201 70 596.3 0 70 202 69 596.3 0 69 203 74 596.3 0 74 204 65 596.3 0 65 205 84 596.3 0 84 206 64 596.3 0 64 207 87 596.3 0 87 208 138 596.3 0 138 209 124 596.3 0 124 210 128 596.3 0 128 211 385 596.3 0 385 212 1373 596.3 0 1373 213 2030 596.3 0 2030 214 2479 596.3 0 2479 215 105 596.3 0 105 216 104 596.3 0 104 217 98 596.3 0 98 218 91 596.3 0 91 219 157 596.3 0 157 220 102 596.3 0 102 221 98 596.3 0 98 222 86 596.3 0 86 223 133 596.3 0 133 224 166 596.3 0 166 225 245 596.3 0 245 226 172 596.3 0 172 227 70 596.3 0 70 228 91 596.3 0 91 229 115 596.3 0 115 230 82 596.3 0 82 231 66 596.3 0 66 232 61 596.3 0 61 233 87 596.3 0 87 234 108 596.3 0 108 235 105 596.3 0 105 236 119 596.3 0 119 237 99 596.3 0 99 238 117 596.3 0 117 239 189 596.3 0 189 240 151 596.3 0 151 241 161 596.3 0 161 242 112 596.3 0 112 243 161 596.3 0 161 244 113 596.3 0 113 245 188 596.3 0 188 246 220 596.3 0 220 247 276 596.3 0 276 248 221 596.3 0 221 249 157 596.3 0 157 250 98 596.3 0 98 251 98 596.3 0 98 252 130 596.3 0 130 253 129 596.3 0 129 254 174 596.3 0 174 255 390 596.3 0 390 256 1429 596.3 0 1429 257 908 596.3 0 908 258 1401 596.3 0 1401 259 281 596.3 0 281 260 230 596.3 0 230 261 209 596.3 0 209 262 178 596.3 0 178 263 153 596.3 0 153 264 152 596.3 0 152 265 104 596.3 0 104 266 437 596.3 0 437 267 552 596.3 0 552 268 1023 596.3 0 1023 269 297 596.3 0 297 270 172 596.3 0 172 271 244 596.3 0 244 272 621 596.3 0 621 273 1103 596.3 0 1103 274 1323 596.3 0 1323 275 955 596.3 0 955 276 254 596.3 0 254 277 230 596.3 0 230 278 309 596.3 0 309 279 239 596.3 0 239 280 426 596.3 0 426 281 343 596.3 0 343 282 304 596.3 0 304 283 215 596.3 0 215 284 277 596.3 0 277 285 169 596.3 0 169 286 206 596.3 0 206 287 466 596.3 0 466 288 372 596.3 0 372 289 487 596.3 0 487 290 120 596.3 0 120 291 117 596.3 0 117 292 156 596.3 0 156 293 316 596.3 0 316 294 251 596.3 0 251 295 218 596.3 0 218 296 122 596.3 0 122 297 136 596.3 0 136 298 92 596.3 0 92 299 92 596.3 0 92 300 153 596.3 0 153 301 316 596.3 0 316 Finished in 1.71 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 52,722 Reads with adapters: 1,328 (2.5%) Reads that were too short: 210 (0.4%) Reads written (passing filters): 1,118 (2.1%) Total basepairs processed: 8,366,201 bp Total written (filtered): 292,105 bp (3.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1328 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 11 0.0 3 0 8 0 3 17 959 0.0 3 893 52 13 1 18 8 0.0 3 0 4 3 1 19 29 0.0 3 15 7 3 4 20 4 0.0 3 0 0 1 3 21 3 0.0 3 0 0 1 2 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 1 27 1 0.0 3 0 0 0 1 33 4 0.0 3 0 0 1 3 34 4 0.0 3 0 0 1 3 35 14 0.0 3 0 0 0 14 36 4 0.0 3 0 0 0 4 37 16 0.0 3 0 0 0 16 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 47 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 3 0.0 3 0 0 0 3 62 21 0.0 3 0 0 0 21 64 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 5 0.0 3 0 0 0 5 71 29 0.0 3 0 0 1 28 72 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 1 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 86 2 0.0 3 0 1 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 112 3 0.0 3 0 0 1 2 113 10 0.0 3 0 0 0 10 114 11 0.0 3 0 0 0 11 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 121 18 0.0 3 0 0 0 18 122 2 0.0 3 0 0 0 2 123 4 0.0 3 0 0 0 4 124 3 0.0 3 0 0 0 3 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 139 4 0.0 3 0 0 0 4 140 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 1 154 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 15 0.0 3 0 0 0 15 164 4 0.0 3 0 0 0 4 165 7 0.0 3 0 0 1 6 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 1 181 1 0.0 3 0 0 0 1 183 9 0.0 3 0 0 0 9 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 243 3 0.0 3 0 0 0 3 263 1 0.0 3 0 0 0 1 276 2 0.0 3 0 0 0 2 277 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,118 Reads with adapters: 49 (4.4%) Reads written (passing filters): 1,118 (100.0%) Total basepairs processed: 292,105 bp Total written (filtered): 291,955 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 49 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.9% C: 16.3% G: 34.7% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 17.5 0 47 4 1 4.4 0 1 5 1 1.1 0 1